antitumor activity of 1 3 h glucan

Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc

Ngày tải lên : 24/03/2014, 04:21

... the exo -1, 3- b-glucanase Substrate (Gn) Km (mM ) 10 3: Vmax (mmol:min 21: mg 21) 10 3: (Vmax/Km) (min 21 : mg 21) ln (Vmax/Km) G3G G3G3G G3G3G3G G3G3G3G3G G3G3G3G3G3G 16 .5 5.0 1. 9 2.0 2 .1 0 .36 5.5 14 16 ... ) Relative activity (%) None Ca 21 Mn 21 Mg 21 Zn 21 Hg 21 PHMB EDTA 10 0 97 13 6 89 83 86 90 81 q FEBS 20 01 Exo -1, 3- b-glucanase from T viride (Eur J Biochem 268) 612 7 in hydrolysis of 1, 3- b-oligoglucosides ... 4000-fold The mode of action of the T viride exo -1, 3- b-glucanase 1 where Km and Vmax are the kinetic parameters of the exo -1, 3b-glucanase-catalyzed hydrolysis of 1, 3- b-oligoglucosides of DP 3 6 Assuming...

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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

Ngày tải lên : 19/02/2014, 06:20

... RNase A, in which the dimeric structure is stabilized through the exchange between subunits of either their N-terminal, or their C-terminal ends [33 35 ] We found that the behavior with the model ... 36 90 Aggregation Fluidity Fusion Positive EIEmax (%) alteration (%) (%) net charge (kT) BS-RNase HHP-RNase RNase AA-GG RNase AA-G RNase AA 10 0 51 71 28 12 10 0 10 3 10 9 58 10 0 10 0 10 0 0 18 10 10 ... through an automated procedure which included the following steps: (a) the model of the membrane was oriented with each layer parallel to the xy plane so that all the phosphorus atoms of each...

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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

Ngày tải lên : 20/02/2014, 02:21

... Biochemistry 42, 714 3 715 1 ´ ´ 30 Ladame, S., Bardet, M., Perie, J & Willson, M (20 01) Selective inhibition of Trypanosoma brucei glyceraldehyde -3- phosphate 31 32 33 34 35 36 37 38 39 40 41 42 43 ... dinucleotide Biochem J 12 2, 59–69 14 Segal, H. L & Boyer, P.D (19 53) The role of sulfhydryl groups in the activity of D-glyceraldehyde -3- phosphate dehydrogenase J Biol Chem 204, 265–2 81 15 Harris, J.I ... D-glyceraldehyde -3- phosphate dehydrogenase Biochem J 37 , 85 51 85 63 ´ ´ Willson, M., Lauth, N., Perie, J., Callens, M & Opperdoes, F (19 94) Inhibition of glyceraldehyde -3- phosphate dehydrogenase by phosphorylated...

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Báo cáo khoa học: Phenylalanine-independent biosynthesis of 1,3,5,8-tetrahydroxyxanthone A retrobiosynthetic NMR study with root cultures of Swertia chirata docx

Ngày tải lên : 08/03/2014, 02:21

... 60.2(4a, 1) 56.4(8a) 1. 5 6.2d 1. 5 1. 4 7.2 6.0 1. 9 1. 6 1. 2 1. 1 1. 2 1. 2 1. 3 1. 0 1. 2 1. 1 1. 1 4.6 1. 2 5.5 1. 5 1. 0d 1. 1 1. 1 1. 1 14 .3 13 JCHa 16 2(2) 16 8(4) 16 0(6), 11 (8) 16 1(7) 4.7 4 .3 4.9 4.4 4.5 13 Determined ... 8.8(6) 4a 4b 16 4 .35 99 .35 16 8.05 15 9 .36 14 5 .16 95.49 13 8 .33 12 4.68 8a 8b 11 0.48 15 4 .37 10 8. 71 102. 81 185.80 a [U- C6]Glucose Coupling constants (Hz) JCCa 73. 0(2), 62.8(8b) 73. 0 (1) , 66 .3( 3) 66.8(4,2) ... 420, 14 3 14 6 10 Lewis, J.R & Gupta, P (19 71) Biosynthesis of xanthones in Gentiana lutea J Chem Soc C, 629–6 31 11 Bennet, G.J & Lee, H. -H (19 88) The biosynthesis of mangostin: the origin of the...

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synthetic applications of 1,3 dipolar cycloaddition chemistry toward heterocycles and natural products

Ngày tải lên : 09/05/2014, 17:06

... OH OH Me Me R1O H2 N CHO ( 13 3) SiMe3 O NHR1 ( 13 4) R1 = R2 = H ( 13 5) R1 = Ac, R2 = Me iii-iv O Me OH O O H Me ( 13 1) ( 13 0) CHO Reagents: i CH2=CHSiMe3, 80 ˚C, 24 h, 90%; ii 50% aq HF, MeCN, rt, 30 ... Me N O OH HO HO HO HO O O OH OH OH OH 29 OH OH (16 0) (15 8) (15 9) Me Me N O O OH O O N R (A) HO O OH O N (16 1) Me (C) Me O OH R O N HO HO HO HO R R= (B) OH OH Reagents: i MeNHOH•HCl, NaHCO3, 80% ... (Scheme 1. 33 ) They found a matched pair of chiral sugar cycloaddition reaction partners to be O O N O 80 ˚C, 12 h C1 1H 23 84% O (1S)- (14 6) O H HO O N O H (R)- (14 5) (14 7) exo Scheme 1. 32 C1 1H2 3...

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Báo cáo toán học: " Antitumor activity of sorafenib-incorporated nanoparticles of dextran/poly(DL-lactide-co-glycolide) block copolymer" pdf

Ngày tải lên : 20/06/2014, 20:20

... (mV) (%, w/w) 40:0 - - - 46 ± 1. 12 36 .4 ± 3 .1 40:2 4.76 1. 23 25.84 63 ± 0.58 35 .5 ± 2.2 40:5 11 .11 4 .36 39 .24 13 3 ± 0.58 36 .0 ± 1. 1 40:7 14 .89 5.26 35 .33 18 1 ± 1. 15 35 .8 ± 0.9 ... a spherical shape with a size < 30 0 nm The higher the initial drug feeding, the higher is the quality of incorporated sorafenib The size of the nanoparticles was increased according to the amount ... administration to rat Int J Pharm 2 011 , 419 :33 9 -34 6 12 Zhang JY, He B, Qu W, Cui Z, Wang YB, Zhang H, Wang JC, Zhang Q: Preparation of the albumin nanoparticle system loaded with both paclitaxel and sorafenib...

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báo cáo hóa học:" Antitumor activity of sorafenib-incorporated nanoparticles of dextran/poly(DL-lactide-co-glycolide) block copolymer" pptx

Ngày tải lên : 21/06/2014, 17:20

... (mV) (%, w/w) 40:0 - - - 46 ± 1. 12 36 .4 ± 3 .1 40:2 4.76 1. 23 25.84 63 ± 0.58 35 .5 ± 2.2 40:5 11 .11 4 .36 39 .24 13 3 ± 0.58 36 .0 ± 1. 1 40:7 14 .89 5.26 35 .33 18 1 ± 1. 15 35 .8 ± 0.9 ... a spherical shape with a size < 30 0 nm The higher the initial drug feeding, the higher is the quality of incorporated sorafenib The size of the nanoparticles was increased according to the amount ... administration to rat Int J Pharm 2 011 , 419 :33 9 -34 6 12 Zhang JY, He B, Qu W, Cui Z, Wang YB, Zhang H, Wang JC, Zhang Q: Preparation of the albumin nanoparticle system loaded with both paclitaxel and sorafenib...

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báo cáo khoa học: " Antitumor activity of mixed heat shock protein/ peptide vaccine and cyclophosphamide plus interleukin-12 in mice sarcoma" pot

Ngày tải lên : 10/08/2014, 10:21

... Experimental & Clinical Cancer Research 2 011 , 30 :24 http://www.jeccr.com/content /30 /1/ 24 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 derived heat-shock protein gp96 after liver resection ... Cancer Research 2 011 , 30 :24 http://www.jeccr.com/content /30 /1/ 24 Page of Table Comparison of antitumor effects of various HSPs Untreated mHSP/p HSP70 HSP60 No of animals tested 10 10 10 10 10 Complete ... mixture of HSP/Ps which, in addition to HSP70 or Gp96, also included HSp60 and HSP 110 The antitumor effects of this mHSP/Ps vaccine were more potent than those of HSP70 or HSP60 alone and of tumor...

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Solid phase synthesis and biological evaluation of 1, 3, 5 triazine derivatives

Ngày tải lên : 14/09/2015, 08:25

... 7 .1 Hz, CO2CH2CH3, 2H) , 2.95 (s, NHCH3, 3H) , 1. 32 (t, J = 7 .1 Hz, CH2CH3, 3H, ); 13 C NMR (CDCl3, 75 MHz):  17 3. 9, 16 2.2, 13 5.9, 12 8.9, 12 8 .3, 12 7.2, 61. 0, 35 .0, 29.7, 14 .2; HRMS (ESI, C1 2H1 6N2O2S ... (m, NCH2CH2Ar, 2H) ; 13 C NMR (CDCl3, 75 MHz):  16 9 .3, 15 2 .1, 15 0 .1, 13 8.0, 13 4.6, 12 9.4, 12 8.9, 12 8.8, 12 8.4, 12 8 .1, 12 6.5, 43. 7, 36 .8, 33 .7, 31 . 1; 13 C DEPT 135 NMR (CDCl3, 75 MHz):  12 9.4, 12 8.9, ... 15 2 .1, 15 0.2, 13 9.4, 13 4.4, 13 4 .3, 12 9.8, 12 9 .3, 12 9.2, 12 8.8, 12 8 .3, 12 8.0, 12 4.7, 36 .9, 31 . 2, 21. 2; HRMS (ESI, C1 8H1 7N3O2S +Na) calcd: 36 2.0 934 , found: 36 2.0 933 32 6-(Benzylthio) -1- methyl -3- phenylethyl -1, 3, 5-triazine-2,4-( 1H, 3H) -dione...

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ỨNG DỤNG MÔ HÌNH GÂY NHIỄM THỰC NGHIỆM CHUẨN ĐÁNH GIÁ ẢNH HƢỞNG CỦA β-1,3/1,6-GLUCAN VÀ VITAMIN C LÊN ĐỘ MẪN CẢM ĐỐI VỚI VIRUS GÂY HỘI CHỨNG ĐỐM TRẮNG (White spot syndrome virus-WSSV) CỦA TÔM SÚ (Penaeus monodon)

Ngày tải lên : 23/04/2013, 15:11

... chất 17 3. 3 Phƣơng pháp nghiên cứu 19 3. 3 .1 Phƣơng pháp pha loãng dịch huyền phù virus 19 3. 3.2 Phƣơng pháp gây nhiễm WSSV tôm sú 19 3. 3 .3 Phƣơng pháp thu mẫu cho ... C 18 41 53 Đối chứng 18 31 38 Nghiệm thức β -1, 3 /1, 6glucan Kết theo dõi thực nghiệm cho thấy, tôm nghiệm thức đối chứng có biểu bất thƣờng xuất sớm sau gây nhiễm WSSV so với nghiệm thức β -1, 3 /1, 6 -glucan ... trúc h a h c -1, 3 /1, 6 -glucan H nh 2.5 Cấu trúc h a h c vitamin C 12 H nh 3 .1 Gây nhiễm WSSV cho tôm cách tiêm 19 Sơ đồ 3 .1 Quy trình tổng quát chẩn đoán IHC 20 H nh 3. 2 H nh...

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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

Ngày tải lên : 14/02/2014, 22:20

... 31 kcat (s )1) 0.56 2.0 2.2 4.6 41 41 69 65 59 40 ± ± ± ± 0.27 0.4 0 .3 1. 2 11 ± ± ± ± ± ± ± Acc Km (mM) 25 12 19 78 12 33 17 13 ± ± ± ± ± ± Acc kcat ⁄ Km (s )1 mM )1) 65 10 0. 53 3.4 2 .1 3. 8 4.5 6.7 ... Approach (Chaplin MF & Kennedy JF eds), pp 1 36 IRL Press, Oxford 33 Schmidt FH (19 61) Die enzymatische Bestimmung von Glucose und Fructose nebeneinander Klin Wochenschr 39 , 12 44 12 47 34 Colson ... mM potassium phosphate (pH 6.8) copy (Fig S1) The spectra confirm that both of the glucans were a- (1 fi 3) -linked glucose polymers The release of the sugars flattened off after 10 0 min, indicating...

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Tài liệu Báo cáo khoa học: Expression of an a-1,3-glucanase during mycoparasitic interaction of Trichoderma asperellum docx

Ngày tải lên : 19/02/2014, 16:20

... (19 72) Morphogenesis in Aspergillus nidulans The signiﬁcance of a alpha -1, 3- glucan of the cell wall and alpha -1, 3- glucanase for cleistothecium development Biochim Biophys Acta 2 73, 17 4 18 7 Schoffelmeer ... mutan (a -1, 3- and a -1, 6 -glucan) , nigeran (a -1, 3- and a -1, 4 -glucan) , soluble starch (a -1, 4- and a -1, 6 -glucan) , pustulan (b -1, 6 -glucan) , laminarin (b -1, 3- glucan) , carboxymethilcelullose (b -1, 4 -glucan) ... a -1, 3- glucanase from T asperellum puriﬁcation of a high number of proteins with this activity To date, there have been described four proteins with a -1, 3- glucanase activity in T harzianum [18 ,24–26]...

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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

Ngày tải lên : 19/02/2014, 16:20

... Prm3 -19 79 - 13 94 -33 08 -10 6 -50 pGL3Basic 10 12 E2 +786 +1 +1 Luc +1 Luc -404 +1 Luc -32 0 +1 Luc -15 4 Luc -15 4 +1 Luc +1 Luc -32 0 Prm3 -19 79 - 13 94 -975 +1 Luc -404 Prm2 E1b -33 08 - 13 94 +1 Luc ... A Prm1 E1 -5895 -8500 Prm2 E1b Prm3 -19 79 - 13 94 -33 08 E2 Prm1 +786 +1 +1 Luc - 13 94 -10 6 -50 +786 +1 Luc -404 +1 Luc -32 0 +1 Luc -15 4 E2 +1 +1 Luc -975 +1 Luc -32 0 Prm3 -19 79 - 13 94 +1 Luc +1 Luc ... AP -1 (-27) +1 - 13 94 -15 4 +1 Luc -14 0 +1 Luc -11 9 -10 6 +1 Luc +1 Luc Luciferase Activity (RLU) B Oct1 ( -12 3) AP -1 Oct1 /2 (-27) ( -10 5) +1 - 13 94 +1 Luc -404 +1 Luc -404 +1 Luc +1 Luc -32 0 +1 Luc...

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Báo cáo khoa học: Anti-HIV-1 activity of 3-deaza-adenosine analogs Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners pot

Ngày tải lên : 08/03/2014, 08:20

... 0.24 0. 83 3.9 28.0 30 .1 50.5 ± ± ± ± ± ± ± ± 0.002 0.008 0.04 0 .15 0.7 4 .1 3. 0 7 .3 Ó FEBS 20 03 Anti-HIV -1 activity of 3- deaza-adenosine analogs (Eur J Biochem 270) 3 511 hydrolase with a Ki of 0.007 ... the phosphorylation of either [ 3H] DZNep or [ 3H] DZAri Therefore, part of the mode of action of these analogs might be similar to that of AZT, which is converted by cellular kinases to AZT triphosphate, ... USA 83, 33 02 33 06 43 Smith, J.D & Ledoux, D.N (19 90) Eﬀect of the methylation inhibitors 3- deazaadenosine and 3- deazaaristeromycin on phosphatidylcholine formation in Tetrahymena Biochim Biophys...

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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt

Ngày tải lên : 15/03/2014, 23:20

... P 212 1 21 a = 60 .33 b = 65 .39 c = 96.49 a = b = c = 90° 0.052 (0. 21) 13 .8 (3. 3) 98.9 (95 .3) 3. 3 (2.6) 30 432 0 . 13 5 0 .16 8 0. 014 1. 35 0.09 3 211 38 . 23 2.00 (2 .11 –2.00) P 212 1 21 a = 58.57 b = 64. 63 ... (0. 43) 17 .4 (3. 7) 10 0.0 (10 0.0) 5.4 (5 .3) 25 035 0 .15 4 0.200 0.020 1. 46 0 .11 32 33 334 21 24 30 8 21 22 19 .85 1. 80 (1. 85 1. 80) P 212 1 21 a = 59.95 b = 65 .32 c = 96.54 a = b = c = 90° 0. 039 (0 .16 ) 14 .2 ... 14 .2 (4.7) 99 .3 (94 .1) 3. 2 (2.4) 35 5 63 0 . 13 6 0 .16 3 0. 012 1. 25 0.08 3 214 24 289 17 19 24 75 38 .19 1. 70 (1. 79 1. 70) P 212 1 21 a = 58.72 B = 64.40 C = 94.87 a = b = c = 90° 0.074 (0. 63) 14 .1 (2.2) 99.8...

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Báo cáo khoa học: A (1ﬁ3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of ﬁbrinogen-like protein and epidermal growth factor gene expression pot

Ngày tải lên : 16/03/2014, 16:20

... (Q14 31 4 ) as an example, together with the techylectins from the horseshoe crab, Tachypleus tridentatus [20] (AB024 737 .1 and AB024 738 .1) , and the angiopoietins from mammals, e.g humans (O1 512 3) , ... and 3) share highest similarity to the domains present in the human EGF (P 011 33 ), the bovine fibrillin (P9 8 13 3) and the Podocoryne carnea (Cnidaria) fibrillin (AAA 9 13 36 ) More closely related to the ... Tenascins: the tenascin precursors from chicken (TENA_CHICK; P10 039 ), human (TENA_HUMAN; P248 21 – and – TENAl_HUMAN; dJ 114 1O19 .1) , fish Danio rerio (TENAC_DARE; CAA 614 89 .1) , and pig (TENA_PIG; Q2 911 6 –...

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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot

Ngày tải lên : 22/03/2014, 16:20

... K-49 (10 0, 33 , 11 , 3. 3, 1. 1 and 0 .33 lM); lanes 914 , plus RT and RDS 16 34 (10 0, 33 , 11 , 3. 3, 1. 1 and 0 .33 lM) (C) LineweaverBurk plot of the inhibition of the HIV -1 RNase H activity by K-49 Reactions ... Asn1 03 Tyr1 81 VAL108A VAL106A LEU 234 A ASN103A TYR188A Tyr1 81 D H O O O K126 Asp 110 VAL108A O O O TYR188A VAL108A O Asp 110 O O O VAL106A Asp186 TYR188A Trp229 Asp186 PHE227A NNRTIbp Asp185 K126 ... Asp185 Tyr188 TYR 31 8 A TRP229A Trp229 LEU 234 A LEU100A Tyr1 81 LEU100A Cys1 81 I VAL108A Asp 110 PHE227A Asp186 Asp185 O O K126 O O O TYR188A Tyr1 81 O LEU 234 A VAL106A LEU100A Trp229 TYR 31 8 A Asn103...

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Báo cáo khoa học: Transcriptional activity and Sp 1⁄3 transcription factor binding to the P1 promoter sequences of the human AbH-J-J locus docx

Ngày tải lên : 23/03/2014, 07:20

... et al 12 13 14 15 16 17 12 fold molar excess of competitor 12 25 12 25 Sp1 /3 Sp3 Sp1 /3 * Sp3 * 23 24 25 26 er r Sp 1m e 31 32 33 34 35 fold 25 50 25 molar excess of competitor 50 25 50 10 0 10 0 ... Units A 10 3 P1 promoter Q PCR MEF2A ChIP Input 10 2 Sp1 ChIP Sp3 ChIP TF 10 1 Nonimmune serum ChIP 16 18 20 22 24 26 28 30 32 34 36 38 Cycle number Fold increase over negative control PCR C Sp1 ChIP ... we cloned a 3 .1 kb partially digested Relative luciferase activity A + 81 -16 83 -12 89 -12 04 -10 17 - 834 -6 61 - 634 - 512 -38 9 -240 -16 0 B 50 15 0 10 0 200 A B C D E F G H I L M 250 275 HepG2 Relative...

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Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf

Ngày tải lên : 29/03/2014, 23:20

... 0.2)0 .3 lm of cytoplasmic Ca2+ [11 ,12 ] Therefore, the mode of regulation by external Ca2+ suggests that BI -1 shows Ca2+ signaling different from that of the conventional InsP3 receptor, even though ... M (19 99) Encoding of Ca2+ signals by differential expression of IP3 receptor subtypes EMBO J 18 , 13 03 13 08 12 Bezprozvanny I, Watras J & Ehrlich BE (19 91) Bell-shaped calcium-response curve of ... 32 3, 10 1 11 3 Kawai-Yamada M, Ohori Y & Uchimiya H (2004) Dissection of Arabidopsis Bax inhibitor -1 suppressing Bax-, hydrogen peroxide-, and salicylic acid-induced cell death Plant Cell 16 , 21 32 ...

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