antitumor activity of 1 3 h glucan

... the exo -1, 3- b-glucanase Substrate (Gn) Km (mM ) 10 3: Vmax (mmol:min 21: mg 21) 10 3: (Vmax/Km) (min 21 : mg 21) ln (Vmax/Km) G3G G3G3G G3G3G3G G3G3G3G3G G3G3G3G3G3G 16 .5 5.0 1. 9 2.0 2 .1 0 .36 5.5 14 16 ... ) Relative activity (%) None Ca 21 Mn 21 Mg 21 Zn 21 Hg 21 PHMB EDTA 10 0 97 13 6 89 83 86 90 81 q FEBS 20 01 Exo -1, 3- b-glucanase from T viride (Eur J Biochem 268) 612 7 in hydrolysis of 1, 3- b-oligoglucosides ... 4000-fold The mode of action of the T viride exo -1, 3- b-glucanase 1 where Km and Vmax are the kinetic parameters of the exo -1, 3b-glucanase-catalyzed hydrolysis of 1, 3- b-oligoglucosides of DP 3 6 Assuming...

Ngày tải lên: 24/03/2014, 04:21

... RNase A, in which the dimeric structure is stabilized through the exchange between subunits of either their N-terminal, or their C-terminal ends [33 35 ] We found that the behavior with the model ... 36 90 Aggregation Fluidity Fusion Positive EIEmax (%) alteration (%) (%) net charge (kT) BS-RNase HHP-RNase RNase AA-GG RNase AA-G RNase AA 10 0 51 71 28 12 10 0 10 3 10 9 58 10 0 10 0 10 0 0 18 10 10 ... through an automated procedure which included the following steps: (a) the model of the membrane was oriented with each layer parallel to the xy plane so that all the phosphorus atoms of each...

Ngày tải lên: 19/02/2014, 06:20

... Biochemistry 42, 714 3 715 1 ´ ´ 30 Ladame, S., Bardet, M., Perie, J & Willson, M (20 01) Selective inhibition of Trypanosoma brucei glyceraldehyde -3- phosphate 31 32 33 34 35 36 37 38 39 40 41 42 43 ... dinucleotide Biochem J 12 2, 59–69 14 Segal, H. L & Boyer, P.D (19 53) The role of sulfhydryl groups in the activity of D-glyceraldehyde -3- phosphate dehydrogenase J Biol Chem 204, 265–2 81 15 Harris, J.I ... D-glyceraldehyde -3- phosphate dehydrogenase Biochem J 37 , 85 51 85 63 ´ ´ Willson, M., Lauth, N., Perie, J., Callens, M & Opperdoes, F (19 94) Inhibition of glyceraldehyde -3- phosphate dehydrogenase by phosphorylated...

Ngày tải lên: 20/02/2014, 02:21

... 60.2(4a, 1) 56.4(8a) 1. 5 6.2d 1. 5 1. 4 7.2 6.0 1. 9 1. 6 1. 2 1. 1 1. 2 1. 2 1. 3 1. 0 1. 2 1. 1 1. 1 4.6 1. 2 5.5 1. 5 1. 0d 1. 1 1. 1 1. 1 14 .3 13 JCHa 16 2(2) 16 8(4) 16 0(6), 11 (8) 16 1(7) 4.7 4 .3 4.9 4.4 4.5 13 Determined ... 8.8(6) 4a 4b 16 4 .35 99 .35 16 8.05 15 9 .36 14 5 .16 95.49 13 8 .33 12 4.68 8a 8b 11 0.48 15 4 .37 10 8. 71 102. 81 185.80 a [U- C6]Glucose Coupling constants (Hz) JCCa 73. 0(2), 62.8(8b) 73. 0 (1) , 66 .3( 3) 66.8(4,2) ... 420, 14 3 14 6 10 Lewis, J.R & Gupta, P (19 71) Biosynthesis of xanthones in Gentiana lutea J Chem Soc C, 629–6 31 11 Bennet, G.J & Lee, H. -H (19 88) The biosynthesis of mangostin: the origin of the...

Ngày tải lên: 08/03/2014, 02:21

... OH OH Me Me R1O H2 N CHO ( 13 3) SiMe3 O NHR1 ( 13 4) R1 = R2 = H ( 13 5) R1 = Ac, R2 = Me iii-iv O Me OH O O H Me ( 13 1) ( 13 0) CHO Reagents: i CH2=CHSiMe3, 80 ˚C, 24 h, 90%; ii 50% aq HF, MeCN, rt, 30 ... Me N O OH HO HO HO HO O O OH OH OH OH 29 OH OH (16 0) (15 8) (15 9) Me Me N O O OH O O N R (A) HO O OH O N (16 1) Me (C) Me O OH R O N HO HO HO HO R R= (B) OH OH Reagents: i MeNHOH•HCl, NaHCO3, 80% ... (Scheme 1. 33 ) They found a matched pair of chiral sugar cycloaddition reaction partners to be O O N O 80 ˚C, 12 h C1 1H 23 84% O (1S)- (14 6) O H HO O N O H (R)- (14 5) (14 7) exo Scheme 1. 32 C1 1H2 3...

Ngày tải lên: 09/05/2014, 17:06

... (mV) (%, w/w) 40:0 - - - 46 ± 1. 12 36 .4 ± 3 .1 40:2 4.76 1. 23 25.84 63 ± 0.58 35 .5 ± 2.2 40:5 11 .11 4 .36 39 .24 13 3 ± 0.58 36 .0 ± 1. 1 40:7 14 .89 5.26 35 .33 18 1 ± 1. 15 35 .8 ± 0.9  ... a spherical shape with a size < 30 0 nm The higher the initial drug feeding, the higher is the quality of incorporated sorafenib The size of the nanoparticles was increased according to the amount ... administration to rat Int J Pharm 2 011 , 419 :33 9 -34 6 12 Zhang JY, He B, Qu W, Cui Z, Wang YB, Zhang H, Wang JC, Zhang Q: Preparation of the albumin nanoparticle system loaded with both paclitaxel and sorafenib...

Ngày tải lên: 20/06/2014, 20:20

... (mV) (%, w/w) 40:0 - - - 46 ± 1. 12 36 .4 ± 3 .1 40:2 4.76 1. 23 25.84 63 ± 0.58 35 .5 ± 2.2 40:5 11 .11 4 .36 39 .24 13 3 ± 0.58 36 .0 ± 1. 1 40:7 14 .89 5.26 35 .33 18 1 ± 1. 15 35 .8 ± 0.9  ... a spherical shape with a size < 30 0 nm The higher the initial drug feeding, the higher is the quality of incorporated sorafenib The size of the nanoparticles was increased according to the amount ... administration to rat Int J Pharm 2 011 , 419 :33 9 -34 6 12 Zhang JY, He B, Qu W, Cui Z, Wang YB, Zhang H, Wang JC, Zhang Q: Preparation of the albumin nanoparticle system loaded with both paclitaxel and sorafenib...

Ngày tải lên: 21/06/2014, 17:20

... Experimental & Clinical Cancer Research 2 011 , 30 :24 http://www.jeccr.com/content /30 /1/ 24 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 derived heat-shock protein gp96 after liver resection ... Cancer Research 2 011 , 30 :24 http://www.jeccr.com/content /30 /1/ 24 Page of Table Comparison of antitumor effects of various HSPs Untreated mHSP/p HSP70 HSP60 No of animals tested 10 10 10 10 10 Complete ... mixture of HSP/Ps which, in addition to HSP70 or Gp96, also included HSp60 and HSP 110 The antitumor effects of this mHSP/Ps vaccine were more potent than those of HSP70 or HSP60 alone and of tumor...

Ngày tải lên: 10/08/2014, 10:21

... 7 .1 Hz, CO2CH2CH3, 2H) , 2.95 (s, NHCH3, 3H) , 1. 32 (t, J = 7 .1 Hz, CH2CH3, 3H, ); 13 C NMR (CDCl3, 75 MHz):  17 3. 9, 16 2.2, 13 5.9, 12 8.9, 12 8 .3, 12 7.2, 61. 0, 35 .0, 29.7, 14 .2; HRMS (ESI, C1 2H1 6N2O2S ... (m, NCH2CH2Ar, 2H) ; 13 C NMR (CDCl3, 75 MHz):  16 9 .3, 15 2 .1, 15 0 .1, 13 8.0, 13 4.6, 12 9.4, 12 8.9, 12 8.8, 12 8.4, 12 8 .1, 12 6.5, 43. 7, 36 .8, 33 .7, 31 . 1; 13 C DEPT 135 NMR (CDCl3, 75 MHz):  12 9.4, 12 8.9, ... 15 2 .1, 15 0.2, 13 9.4, 13 4.4, 13 4 .3, 12 9.8, 12 9 .3, 12 9.2, 12 8.8, 12 8 .3, 12 8.0, 12 4.7, 36 .9, 31 . 2, 21. 2; HRMS (ESI, C1 8H1 7N3O2S +Na) calcd: 36 2.0 934 , found: 36 2.0 933 32 6-(Benzylthio) -1- methyl -3- phenylethyl -1, 3, 5-triazine-2,4-( 1H, 3H) -dione...

Ngày tải lên: 14/09/2015, 08:25

... chất 17 3. 3 Phƣơng pháp nghiên cứu 19 3. 3 .1 Phƣơng pháp pha loãng dịch huyền phù virus 19 3. 3.2 Phƣơng pháp gây nhiễm WSSV tôm sú 19 3. 3 .3 Phƣơng pháp thu mẫu cho ... C 18 41 53 Đối chứng 18 31 38 Nghiệm thức β -1, 3 /1, 6glucan Kết theo dõi thực nghiệm cho thấy, tôm nghiệm thức đối chứng có biểu bất thƣờng xuất sớm sau gây nhiễm WSSV so với nghiệm thức β -1, 3 /1, 6 -glucan ... trúc h a h c -1, 3 /1, 6 -glucan H nh 2.5 Cấu trúc h a h c vitamin C 12 H nh 3 .1 Gây nhiễm WSSV cho tôm cách tiêm 19 Sơ đồ 3 .1 Quy trình tổng quát chẩn đoán IHC 20 H nh 3. 2 H nh...

Ngày tải lên: 23/04/2013, 15:11

... 31 kcat (s )1) 0.56 2.0 2.2 4.6 41 41 69 65 59 40 ± ± ± ± 0.27 0.4 0 .3 1. 2 11 ± ± ± ± ± ± ± Acc Km (mM) 25 12 19 78 12 33 17 13 ± ± ± ± ± ± Acc kcat ⁄ Km (s )1 mM )1) 65 10 0. 53 3.4 2 .1 3. 8 4.5 6.7 ... Approach (Chaplin MF & Kennedy JF eds), pp 1 36 IRL Press, Oxford 33 Schmidt FH (19 61) Die enzymatische Bestimmung von Glucose und Fructose nebeneinander Klin Wochenschr 39 , 12 44 12 47 34 Colson ... mM potassium phosphate (pH 6.8) copy (Fig S1) The spectra confirm that both of the glucans were a- (1 fi 3) -linked glucose polymers The release of the sugars flattened off after 10 0 min, indicating...

Ngày tải lên: 14/02/2014, 22:20

... (19 72) Morphogenesis in Aspergillus nidulans The significance of a alpha -1, 3- glucan of the cell wall and alpha -1, 3- glucanase for cleistothecium development Biochim Biophys Acta 2 73, 17 4 18 7 Schoffelmeer ... mutan (a -1, 3- and a -1, 6 -glucan) , nigeran (a -1, 3- and a -1, 4 -glucan) , soluble starch (a -1, 4- and a -1, 6 -glucan) , pustulan (b -1, 6 -glucan) , laminarin (b -1, 3- glucan) , carboxymethilcelullose (b -1, 4 -glucan) ... a -1, 3- glucanase from T asperellum purification of a high number of proteins with this activity To date, there have been described four proteins with a -1, 3- glucanase activity in T harzianum [18 ,24–26]...

Ngày tải lên: 19/02/2014, 16:20

... Prm3 -19 79 - 13 94 -33 08 -10 6 -50 pGL3Basic 10 12 E2 +786 +1 +1 Luc +1 Luc -404 +1 Luc -32 0 +1 Luc -15 4 Luc -15 4 +1 Luc +1 Luc -32 0 Prm3 -19 79 - 13 94 -975 +1 Luc -404 Prm2 E1b -33 08 - 13 94 +1 Luc ... A Prm1 E1 -5895 -8500 Prm2 E1b Prm3 -19 79 - 13 94 -33 08 E2 Prm1 +786 +1 +1 Luc - 13 94 -10 6 -50 +786 +1 Luc -404 +1 Luc -32 0 +1 Luc -15 4 E2 +1 +1 Luc -975 +1 Luc -32 0 Prm3 -19 79 - 13 94 +1 Luc +1 Luc ... AP -1 (-27) +1 - 13 94 -15 4 +1 Luc -14 0 +1 Luc -11 9 -10 6 +1 Luc +1 Luc Luciferase Activity (RLU) B Oct1 ( -12 3) AP -1 Oct1 /2 (-27) ( -10 5) +1 - 13 94 +1 Luc -404 +1 Luc -404 +1 Luc +1 Luc -32 0 +1 Luc...

Ngày tải lên: 19/02/2014, 16:20

... 0.24 0. 83 3.9 28.0 30 .1 50.5 ± ± ± ± ± ± ± ± 0.002 0.008 0.04 0 .15 0.7 4 .1 3. 0 7 .3 Ó FEBS 20 03 Anti-HIV -1 activity of 3- deaza-adenosine analogs (Eur J Biochem 270) 3 511 hydrolase with a Ki of 0.007 ... the phosphorylation of either [ 3H] DZNep or [ 3H] DZAri Therefore, part of the mode of action of these analogs might be similar to that of AZT, which is converted by cellular kinases to AZT triphosphate, ... USA 83, 33 02 33 06 43 Smith, J.D & Ledoux, D.N (19 90) Effect of the methylation inhibitors 3- deazaadenosine and 3- deazaaristeromycin on phosphatidylcholine formation in Tetrahymena Biochim Biophys...

Ngày tải lên: 08/03/2014, 08:20

... P 212 1 21 a = 60 .33 b = 65 .39 c = 96.49 a = b = c = 90° 0.052 (0. 21) 13 .8 (3. 3) 98.9 (95 .3) 3. 3 (2.6) 30 432 0 . 13 5 0 .16 8 0. 014 1. 35 0.09 3 211 38 . 23 2.00 (2 .11 –2.00) P 212 1 21 a = 58.57 b = 64. 63 ... (0. 43) 17 .4 (3. 7) 10 0.0 (10 0.0) 5.4 (5 .3) 25 035 0 .15 4 0.200 0.020 1. 46 0 .11 32 33 334 21 24 30 8 21 22 19 .85 1. 80 (1. 85 1. 80) P 212 1 21 a = 59.95 b = 65 .32 c = 96.54 a = b = c = 90° 0. 039 (0 .16 ) 14 .2 ... 14 .2 (4.7) 99 .3 (94 .1) 3. 2 (2.4) 35 5 63 0 . 13 6 0 .16 3 0. 012 1. 25 0.08 3 214 24 289 17 19 24 75 38 .19 1. 70 (1. 79 1. 70) P 212 1 21 a = 58.72 B = 64.40 C = 94.87 a = b = c = 90° 0.074 (0. 63) 14 .1 (2.2) 99.8...

Ngày tải lên: 15/03/2014, 23:20

... (Q14 31 4 ) as an example, together with the techylectins from the horseshoe crab, Tachypleus tridentatus [20] (AB024 737 .1 and AB024 738 .1) , and the angiopoietins from mammals, e.g humans (O1 512 3) , ... and 3) share highest similarity to the domains present in the human EGF (P 011 33 ), the bovine fibrillin (P9 8 13 3) and the Podocoryne carnea (Cnidaria) fibrillin (AAA 9 13 36 ) More closely related to the ... Tenascins: the tenascin precursors from chicken (TENA_CHICK; P10 039 ), human (TENA_HUMAN; P248 21 – and – TENAl_HUMAN; dJ 114 1O19 .1) , fish Danio rerio (TENAC_DARE; CAA 614 89 .1) , and pig (TENA_PIG; Q2 911 6 –...

Ngày tải lên: 16/03/2014, 16:20

... K-49 (10 0, 33 , 11 , 3. 3, 1. 1 and 0 .33 lM); lanes 914 , plus RT and RDS 16 34 (10 0, 33 , 11 , 3. 3, 1. 1 and 0 .33 lM) (C) LineweaverBurk plot of the inhibition of the HIV -1 RNase H activity by K-49 Reactions ... Asn1 03 Tyr1 81 VAL108A VAL106A LEU 234 A ASN103A TYR188A Tyr1 81 D H O O O K126 Asp 110 VAL108A O O O TYR188A VAL108A O Asp 110 O O O VAL106A Asp186 TYR188A Trp229 Asp186 PHE227A NNRTIbp Asp185 K126 ... Asp185 Tyr188 TYR 31 8 A TRP229A Trp229 LEU 234 A LEU100A Tyr1 81 LEU100A Cys1 81 I VAL108A Asp 110 PHE227A Asp186 Asp185 O O K126 O O O TYR188A Tyr1 81 O LEU 234 A VAL106A LEU100A Trp229 TYR 31 8 A Asn103...

Ngày tải lên: 22/03/2014, 16:20

... et al 12 13 14 15 16 17 12 fold molar excess of competitor 12 25 12 25 Sp1 /3 Sp3 Sp1 /3 * Sp3 * 23 24 25 26 er r Sp 1m e 31 32 33 34 35 fold 25 50 25 molar excess of competitor 50 25 50 10 0 10 0 ... Units A 10 3 P1 promoter Q PCR MEF2A ChIP Input 10 2 Sp1 ChIP Sp3 ChIP TF 10 1 Nonimmune serum ChIP 16 18 20 22 24 26 28 30 32 34 36 38 Cycle number Fold increase over negative control PCR C Sp1 ChIP ... we cloned a 3 .1 kb partially digested Relative luciferase activity A + 81 -16 83 -12 89 -12 04 -10 17 - 834 -6 61 - 634 - 512 -38 9 -240 -16 0 B 50 15 0 10 0 200 A B C D E F G H I L M 250 275 HepG2 Relative...

Ngày tải lên: 23/03/2014, 07:20

... 0.2)0 .3 lm of cytoplasmic Ca2+ [11 ,12 ] Therefore, the mode of regulation by external Ca2+ suggests that BI -1 shows Ca2+ signaling different from that of the conventional InsP3 receptor, even though ... M (19 99) Encoding of Ca2+ signals by differential expression of IP3 receptor subtypes EMBO J 18 , 13 03 13 08 12 Bezprozvanny I, Watras J & Ehrlich BE (19 91) Bell-shaped calcium-response curve of ... 32 3, 10 1 11 3 Kawai-Yamada M, Ohori Y & Uchimiya H (2004) Dissection of Arabidopsis Bax inhibitor -1 suppressing Bax-, hydrogen peroxide-, and salicylic acid-induced cell death Plant Cell 16 , 21 32 ...

Ngày tải lên: 29/03/2014, 23:20