antihypertensive properties of plant protein derived peptides
Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
Ngày tải lên :
21/02/2014, 15:20
... them with sequences of cationic peptides in the venom of other arthropods EXPERIMENTAL PROCEDURES Collection of venom Venom of O carinatus was collected by electrical stimulation of the telson with ... Determination of synergism of cationic peptides with conventional antibiotics Twofold serial dilutions of amoxicillin, levofloxacin, cefuroxime and erythromycin were tested in the presence of a constant ... activity of the peptides was assayed by microspectrophotometry of liquid cultures grown in microtiter plates as described previously [33] Briefly, in one well of a 96-well microplate, 20 lL of the protein...
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Báo cáo khóa học: Structural properties of the protein SV-IV potx
Ngày tải lên :
07/03/2014, 14:20
... screen the whole protein sequence to identify the acetylated peptides Peptides were identified by their molecular mass on the basis of the known protein sequence and the endoproteinase Glu-C specificity ... mixture of the native and acetylated peptides was observed and identified by the mass increase of 42 mass units The relative level of acetylation of a peptide was estimated on the basis of the ... endoproteinase Glu-C digest of acetylated protein SV-IV Signal diagnostics of peptide structure are indicated in the figure Fig Molecular model of the peptide corresponding to the 70–90 region of...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
Ngày tải lên :
16/03/2014, 16:20
... mixture of 10 mL taken from each of the five reaction mixtures The method consists of adding various iodoacetamide and iodoacetate ratios to portions of the protein to generate a complete spectrum of ... or presence of ATP, show that ATP has little or no effect on this activity Interestingly, comparison of the genomes of archaea and bacteria showed the existence of a group of redox proteins with ... Assessment of the ATP binding properties of Hsp90 J Biol Chem 271, 10035–10041 35 Quemeneur, E., Guthapfel, R & Gueguen, P (1994) A major phosphoprotein of the endoplasmic reticulum is protein disulfide...
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gas sensing properties of metal - organics derived pt dispersed - tio2 thin film fired in nh3
Ngày tải lên :
19/03/2014, 16:48
... B 67 (2000) 270–274 Fig Change of gas sensitivity with firing temperature This paper described the effect of NH on gas sensing properties of Pt dispersed-TiO2 derived from precursor Experimental ... linking medium of Ti and Pt Then, an excess amount of water was added to hydrolyze the residual alkoxy groups of TiŽO-iPr ŽAcAc The amount of platinum salt was adjusted to the composition of wt.% ... resistance of 1H is smaller than that of 2H, the resistance of 1a is larger than that of 2a It turns out that the TiO –Pt film is a little more reduced than the TiO film, but the resistance of the...
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Synthesis and Physical Properties of Polyester Amides Derived from Lipid-Based Components
Ngày tải lên :
02/07/2014, 13:01
... Study the effects of crystallinity on the physical properties of the PEAs Prepare a PEA with an ester to amide ratio of 4:1 in order to model the trend observed in the physical properties versus ... structure resulted in a decrease of thermal stability a The increase of ester to amide ratio did not impact dramatically the thermal degradation of the PEAs b The increase of ester to amide ratio decreased ... biodegradability of PEAs with varying ratios of ester to amide groups Increase the molecular weights of the synthesized PEAs to further improve the thermal and mechanical properties (such as...
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Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
Ngày tải lên :
09/08/2014, 07:20
... the absence of infiltration of immunocompetent cells and tissue destruction (Fig 1d) Histological examination of joint sections of AMD3100-treated mice that did show clinical symptoms of arthritis ... Table Inhibition of the incidence and mean score of CIA by treatment with AMD3100 Experiment number Treatmenta Cumulative incidence (%) Start of treatmentb End of treatmentc Score of arthritis (mean ... of the pits was performed at a magnification of ×20 in 15 areas of constant size, positioned adjacent to one another and spanning the whole quartz slide In all slides, the minimum threshold of...
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Immunomodulatory properties of polysaccharide protein complex from lycium barbarum l
Ngày tải lên :
11/09/2015, 16:03
... IMMUNOMODULATORY PROPERTIES OF POLYSACCHARIDEPROTEIN COMPLEX FROM LYCIUM BARBARUM L CHEN ZHISONG B.Med.; M.Med A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY ... Characterization of LBP 83 84 3.1.1 Aim of Study 84 3.1.2 Results 84 3.1.2.1 Isolation of LBP 84 3.1.2.2 Purification of LBP 85 3.1.2.3 Characterization of LBP on Carbohydrate and Protein Content ... List of Tables LIST OF TABLES Table LBP composition, structure and molecular weight 10 Table Carbohydrate and protein content and molecular weight of LBPF1-5 91 Table Test of LPS contamination of...
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Báo cáo khoa học: Kininogen-derived peptides for investigating the putative vasoactive properties of human cathepsins K and L docx
Ngày tải lên :
31/03/2014, 07:20
... Fig Hydrolysis of kininogen -derived peptides and kinins by hK1 and cathepsins B, L and K (A) Structure of kininogen -derived fluorogenic substrates The sequence surrounding the region of bradykinin ... assembly of the head of bacteriophage T4 Nature 227, 680–685 29 Chagas, J.R., Authie, E., Serveau, C., Lalmanach, G., Juliano, L & Gauthier, F (1997) A comparison of the enzymatic properties of the ... Juliano, L & Gauthier, F (1996) Investigation of the substrate specificity of cruzipain, the major cysteine proteinase of Trypanosoma cruzi, though the use of cystatin-derivated substrates and inhibitors...
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Báo cáo y học: "Salivary gland derived peptides as a new class of anti-inflammatory agents: review of preclinical pharmacology of C-terminal peptides of SMR1 protein" pptx
Ngày tải lên :
11/08/2014, 03:20
... Mathison et al.: Salivary gland derived peptides as a new class of anti-inflammatory agents: review of preclinical pharmacology of C-terminal peptides of SMR1 protein Journal of Inflammation 2010 7:49 ... Journal of Inflammation 2010, 7:49 http://www.journal-inflammation.com/content/7/1/49 Page of 11 Intravenous injection of feG 20 minutes before LPS dose-dependently reduced the length of time of disruption ... within a window of concentrations of about 0.0035 to 0.35 ng/mL, and that in model studies in rats feG dosage of 100 μg/kg was consistently found to be effective regardless of route of administration,...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
Ngày tải lên :
18/02/2014, 14:20
... characterizing proteins interacting with MCPIP will clarify the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level After identification of a PIN ... UTR of IL-6 mRNA [4,17] The presence of such a sequence may account for the degra- MCPIP protein as an RNase dation of both types of IL-1b transcripts, with and without AREs The influence of MCPIP ... analyses to identify putative proteins interacting with MCPIP (data not shown) Of the identified proteins, 73% are proteins involved in mRNA stability, but there are also proteins involved in other...
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Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf
Ngày tải lên :
18/02/2014, 16:20
... concomitant appearance of other signals in other parts of the spectrum This result could be a consequence of the paramagnetic properties of Fe(II), or it might arise from the presence of an intermediate ... behavior of Fe(II), because of the similarity of ionic radii and paramagnetic properties [31] It should produce efficient line broadening of ˚ atoms closer than A Accordingly, complete bleaching of ... Representative HSQC spectra of CyaY titrations Superimpositions of a reference [1H,15N] HSQC spectrum of CyaY (blue) with a spectrum (red) of a : mixture of CyaY ⁄ Gd3+ (A), a : mixture of CyaY ⁄ Lu3+ (B),...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
Ngày tải lên :
19/02/2014, 13:20
... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... studies of peptides to CD58 protein were carried out in order to understand peptide protein interactions by using autodock [29] In the docking of CD2 peptides to CD58 Ó FEBS 2004 Design of peptides ... topologies of the turn region mimic those of rat CD2 Structure of cyclic hexapeptides – cEL peptide The chemical shifts of amide resonances of peptide cEL were well dispersed over a region of 1.2...
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Tài liệu Báo cáo khoa học: Binding of ligands originates small perturbations on the microscopic thermodynamic properties of a multicentre redox protein pptx
Ngày tải lên :
19/02/2014, 17:20
... in favour of a specific binding of phosphate to cytochrome c3 [23] instead of a simple electrostatic effect of increased ionic strength at least up to 0.2 m concentration The region of positively ... the two proteins are located in different cellular compartments, but it provides a convenient model for studying the effect of partner binding on the thermodynamic properties of the haems of cytochrome ... ligated proteins Fig The pH dependence of the chemical shift of haem methyl resonances 21CH3I , 181CH3II , 121CH3III and 181CH3IV , of DvHc3:Pi at 297.3 K Squares correspond to stage of oxidation,...
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Tài liệu Báo cáo Y học: Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin doc
Ngày tải lên :
22/02/2014, 04:20
... consequence of the presence of the same kind of spectrum for 4N complexes of GGH, where the a carbon of the His residue is the sole source of chirality [10] However, while positions of the component ... studies of the Ni(II) complex of HSA [6] Due to different protonation patterns, the stability constants of particular complexes of model peptides cannot be compared directly There are two ways of ... spectra of albumin complexes clearly indicates the inuence of the whole protein, which can only be transferred via the limitation of conformational freedom of the complex moiety The CD spectra of...
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Báo cáo khoa học: Ion-binding properties of Calnuc, Ca2+ versus Mg2+ – Calnuc adopts additional and unusual Ca2+-binding sites upon interaction with G-protein pdf
Ngày tải lên :
07/03/2014, 00:20
... reported the affinities (Kd of 47 and 40 lm) of Ca2+ for peptides comprising the EF-hands of Calnuc [42], there are no reports of the affinity of the metal ion for the protein as a whole We show ... the ultimate proof of interactions between two proteins in solution, and provide a direct route to the complete thermodynamic characterization of protein interactions Protein protein interactions ... purification of Calnuc The gel also shows the purity of G -protein a-subunit (B) Fluorescence-based activation assay of the G -protein a-subunit Table S1 Comparison of EF-hand sequence of Calnuc with...
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
Ngày tải lên :
07/03/2014, 11:20
... is similar to that of the family 19 chitinase from barley, the best studied of the plant chitinases [9,12,13,27,28] and essentially identical to that of the catalytic domain of ChiC from S griseus ... hypothesis is not confirmed by the present analysis of enzymatic properties of ChiG, or by our analysis of the ChiG and ChiC structures Despite the deletion of the B-loop, the two putative catalytic residues ... the exploitation of chitinous substrates as a source of nutrition Production of multiple enzymes with varying properties (endo-action or exo-action, processive or not, presence of additional substrate-binding...
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Báo cáo khóa học: Some properties of human small heat shock protein Hsp20 (HspB6) potx
Ngày tải lên :
07/03/2014, 15:20
... creation of the so-called 3D mutant of Hsp27 with replacements of Ser15, Ser78 and Ser82 by Asp This type of mutation mimics phosphorylation of Hsp27 by mitogen activated protein kinaseactivated protein ... The protein composition of the fractions obtained in the course of size exclusion chromatography was analyzed by means of SDS gel electrophoresis [33] The effect of pH on the oligomeric state of ... from L of the E coli culture When the S16D mutant of Hsp20 was expressed in E coli, most of the protein was insoluble in the lysis buffer and this buffer extracted less than 20% of the protein...
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Báo cáo khoa học: Adhesion properties of adhesion-regulating molecule 1 protein on endothelial cells pptx
Ngày tải lên :
07/03/2014, 17:20
... modifications of cytosolic proteins [25–26] which not change the molecular mass of proteins as much as complex glycans Interestingly, the human homologous protein of ARM-1 has a molecular mass of 110 ... lymphocytes, the expression of which is up-regulated in metastatic cancer cells This protein does not belong to any of the known families of cell adhesion molecules Homologous proteins are FEBS Journal ... adhesion-regulating properties of ARM-1 ARM-1 is a secreted and cell-associated protein As ARM-1 protein has a putative signal peptide at the N-terminus, we investigated whether it was a secreted protein...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... II (PSII) of C reinhardtii [16] We now report the expression of His- and Strep-tagged plant PSI Results A series of cassettes containing tagged variants of the full-length precursor of Arabidopsis ... from pools of plants expressing similar levels of PSI-G, and analysed by immunoblotting, using antibodies to PSI-G and PSI-F, as an indicator of the relative content of PSI in the samples Plants ... loop-tagged versions of PSI-G This may suggest that the loop of PSI-G is important for stable integration of the subunit into the PSI complex A protein band smaller that the tagged versions of PSI-G is...
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