antibodies antinuclear anti dna and anticentromere

... distribution of anti- NPM and aCL antibodies in these SLE sera (Table 1) indicates that these autoantibodies were associated (χ2 = 9.2; P = 0.002) In contrast, ANA, anti- DNA and anti- β2GPI antibodies ... by the presence of anti- nucleolar antibodies, had anti- NPM and aCL antibodies and SLE However, our results, showing that anti- NPM antibodies are constantly detected in WB mice and frequently observed ... NZB and NZW mice also did not develop anti- NPM antibodies In contrast, anti- NPM antibodies were present in 60% of 20 sera from MRLlpr/lpr mice more than three months old Anti- NPM and aCL antibodies...

Ngày tải lên: 09/08/2014, 07:20

... suggested that anti- double-stranded DNA (anti- dsDNA) antibodies were the main autoantibodies involved in the pathogenesis of LN It has been argued that high-avidity anti- dsDNA antibodies are particularly ... per week, and high-avidity anti- dsDNA antibodies decreased by 0.8% per week Anti- α-actinin antibodies decreased by 0.0015 per week, which (given the mean baseline AR for anti- α-actinin antibodies ... correlation between anti- nucleosome and high-avidity anti- dsDNA levels (r = 0.6, P = 0.0001) but no correlation between anti- nucleosome and anti- α-actinin or high-avidity anti- dsDNA and anti- α-actinin...

Ngày tải lên: 09/08/2014, 14:22

... all the feces DNA/ RNA isolation and HIV1 detection work and drafted the manuscript LC, MD and CS performed urine DNA/ RNA isolation and HIV-1 detection, the molecular genetic studies and help to ... ethanol and air dried The DNA was re-hydrated in nuclease free water and stored at -20°C for subsequent PCR work Nested PCR and RT-PCR Nested PCR and RT-PCR were performed on isolated RNA /DNA samples ... on the presence of HIV-1 in urine The presence of anti- HIV-1 antibodies has been reported in urine by ELISA and Western blot [15,16] and HIV-1 DNA has been detected in urine pellets from HIV-1...

Ngày tải lên: 10/08/2014, 05:21

... patients with no nephritis [12] and that pathogenic human anti- dsDNA antibodies bound strongly to α-actinin [12,13] Moreover, an increase in serum anti- α-actinin antibodies in lupus patients was ... 2.5-fold increase in the prevalence of LN [14] Finally, levels of anti- α-actinin antibodies correlated with those of anti- DNA antibodies and were significantly higher in patients with renal flares ... lupus In their prospective study, Manson and colleagues [1] directly compared the titers of anti- α-actinin, high-avidity anti- dsDNA, and anti- nucleosome antibodies in 16 patients with new onset...

Ngày tải lên: 12/08/2014, 11:22

... Anti Flood and Malware I Flood Mail II.Hiểm Họa Malware III Cách Phòng Chống Phần I Flood Email 1) Flood ... ngưỡng cho phép vào Blacklist 11 Phần III Cách Phòng Chống 2) Chống Malware: - Thiết lập trình Antivirus – Malware cho Client : VD : NOD32 ESET SMART SECURITY 12 Phần III Cách Phòng Chống 2) ... file không rõ nguồn gốc thực thi + Không click vào link không rõ nguồn gốc + Dùng chương trình Antivirus để quét online offline trước thực thi file 13 Phần III Cách Phòng Chống 2) Chống Malware:...

Ngày tải lên: 14/09/2012, 08:40

... induction of anti- DNA antibodies B19 induced apoptosis generates nucleosomes and apoptotic bodies containing NS1 modified DNA Anergized anti- DNA B cells take up NS1 modified nucleosomal DNA through ... suggested by the finding of NS1 -DNA attachment concerns the development of autoantibodies after B19 infection B19 infection often results in the production of anti- DNA autoantibodies (3, 4) A viral ... apoptosis ATM principally binds to free DNA ends or DNA strand breaks (43), while ATR recognizes single-stranded regions of DNA common to multiple types of DNA lesions and that are often caused by collapsed...

Ngày tải lên: 25/10/2012, 11:18

... following antibodies (BD Biosciences, San Jose, CA): murine anti- human antibodies, FITC-conjugated anti- CD14, PE-conjugated anti- CD16 (3G8; PharMingen), PerCP-conjugated anti- HLA-DR, and isotype ... clades A, E, and B; and demonstrated equivalent amplification of the target gene Separation of PBMC Subsets and HIV DNA Analaysis Because HIV DNA is present in both lymphocytes and monocytes, ... 72°C/2 We used non-HIV-1 infected genomic DNA for a negative control and DNA from three HIV-1 infected cell lines (8E5, OM10.1, and ACH-2; NIH AIDS Research and Reference Reagent Program, NIH, Bethesda,...

Ngày tải lên: 31/10/2012, 15:34

... G-quadruplex DNA (A) EMSA was performed with EWS (lanes and 4) and 32P-labeled ETS-1 (lanes and 4) or ssDNA L (lanes and 2) (B) EMSA was performed with EWS (lanes 2, and 6) and 32P-labeled Htelo (lanes and ... RGG3 (lanes and 4) and 32P-labeled Htelo (lanes and 2) or rHtelo (lanes and 4) (B) EMSA was performed with RGG3 (lanes and 4) and 32P-labeled rHtelo (lanes and 2) or mut rHtelo (lanes and 4) (C, ... and 4), dsHtelo (lanes and 6) or ssDNA S (lanes and 2) The structures of DNAs used as probes are indicated above each lane The DNA protein complexes were resolved by 6% PAGE and visualized by autoradiography...

Ngày tải lên: 15/02/2014, 01:20

... intensity was  45% of its initial abundance DNA strand break analysis The ability of Cu(II)-Ami to induce strand breaks in plasmid DNA in the absence and presence of H2O2 was tested with the use ... antibiotic or its copper(II) complex over tRNAPhe (lanes and 9, Fig 6) The cleavage intensities increased with the concentration of Ami and Cu(II)-Ami gradually, up to their Ó FEBS 2002 DNA and ... tRNAPhe (lanes 20 and 21, Fig 6) In this case, three major cleavages were observed, at Y37, A36 and D17 It has to be noted, however, that the cleavage at D17 and in the D and anticodon loops occurred...

Ngày tải lên: 21/02/2014, 01:21

... Therefore, systemic and extensive investigations are still required to assign universally appropriate parameter sets of the temperature-dependent thermodynamics for the DNA/ DNA and RNA /DNA oligonucleotide ... the present study, we determined the temperatureindependent and temperature-dependent thermodynamic parameters of 24 DNA/ DNA and 41 RNA /DNA oligonucleotide duplexes The heat capacity changes were ... AND METHODS Material preparations DNA and RNA oligonucleotides were synthesized on a solid support using the standard phosphoramidite method with an Applied Biosystems Model 391 synthesizer and...

Ngày tải lên: 22/02/2014, 07:20

... mandarin (Fortunella japonica) is used to prepare traditional remedies for treatment of respiratory and digestive diseases, but its anti- obesity effect is not studied yet In this report the anti- obesity ... fed with standard diet and drinking water ad libitum + Lot 2: Obese mice treated with ethanol fraction and drinking water ad libitum + Lot 3: Obese mice treated with chloroform fraction and drinking ... fraction and drinking water ad libitum + Lot 5: Obese mice treated with metformin (500mg/kg) and drinking water ad libitum + Lot 6: Obese mice fed normal diet and drink water (no treatment) and drinking...

Ngày tải lên: 05/03/2014, 11:21

... traditional remedies for treatment of in and infectious diseases and digestive, but its anti- obesity effect is not studied yet [7] In this report the antiobesity effect of Docynia indica (W.) ... Science, Natural Sciences and Technology 27 (2011) 125-133 Introduction Obesity can be considered as an abnormal state of lipid metabolism and increasing in developed and even developing countries ... National Institute of Hygiene and Epidemiology (NIHE) were housed in a temperature (25 ± 20C) and humidity (80% ± 10 %) under a 12h light/12 h dark cycle Diets and tap water were provided ad...

Ngày tải lên: 05/03/2014, 11:21

... relative strand orientations, being respectively: (a) anti anti anti anti or syn•syn•syn•syn, (b) syn anti anti anti or anti syn• syn•syn, (c) syn•syn anti anti and (d) syn anti syn• anti The hydrogen-bond ... one strand and 5¢-syn-syn -anti- 3¢ and 5¢-syn -anti- anti-3¢ for the other strand of the dimer; glycosidic conformations of guanines around G-tetrads are syn anti syn anti; there are two wide and two ... along G-tracts are 5¢-syn -anti- anti-3¢ and 5¢-syn-syn -anti- 3¢; there are two syn anti anti anti G-tetrads and one anti syn•syn•syn G-tetrad; there are one narrow, one wide and two medium grooves...

Ngày tải lên: 06/03/2014, 09:22

... tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000) The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis ... temporally and spatially separate DNA unwinding by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and ... interaction between Net1 and Sir2 on one hand and between Net1 and Fob1 on the other, and loads Sir2 near the Ter sites This process, as noted above, causes silencing of rDNA and prevents unwanted...

Ngày tải lên: 06/03/2014, 23:20

... NaH2PO4, and KI were obtained either from Fluka (Buchs, Switzerland) or from Riedel-deHaen (Seelze GmbH, Germany) dNTP, Pwo DNA polyă merase, HindIII and EcoRI endonucleases, and T4 DNA ligase ... articial ICAP DNA possesses a symmetrical sequence, which binds with high afnity to the CRP HTH motifs, and the lac and gal DNA sequences represent the CRP-binding sites from class I and class II ... emission and has a maximum at 338 nm 1105 CRP conformational changes induced by DNA and cAMP M Tworzydo et al Table Fluorescence quenching parameters for CRPW85A, CRPW85A(cAMP)2 and CRPW85ADNA complexes...

Ngày tải lên: 07/03/2014, 16:20

... Polarized strand, 5’->3’ Base inside, sugar outside DNA and its Building Antiparallel strands DNA Pairs A always pairs with T, and G with C, A-T two hydrogen bonds, G-C three hydrogen bonds DNA Double ... template to form complimentary pairs with a new DNA strand • DNA is in nucleus in eucaryotes 1953 Watson and Crick determined the structure of DNA DNA and its Building Nucleotides: Guanine (G), Adenine ... strands holding together by hydrogen bonds between A-T (2 bonds) and G-C (3 bonds) • DNA duplication occurs using one strand of parental DNA as template to form complimentary pairs with a new DNA...

Ngày tải lên: 13/03/2014, 16:39

... tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB (Kaplan, 2000) The translocation of DnaB on double-stranded DNA (dsDNA) requires ATP hydrolysis ... temporally and spatially separate DNA unwinding by DnaB helicase from its ATP-dependent locomotion on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and ... interaction between Net1 and Sir2 on one hand and between Net1 and Fob1 on the other, and loads Sir2 near the Ter sites This process, as noted above, causes silencing of rDNA and prevents unwanted...

Ngày tải lên: 17/03/2014, 21:20

... Strategy to isolate human neutralizing antibodies large antibody repertoires From these libraries, specific antibodies can be selected by linking phenotype (binding affinity) ... Fig Specificity of phage -antibodies 3F and C1 (A) Cross-reactivity: scFv 3F (hatched boxes) and scFv C1 (empty boxes) ELISA was used to determine binding to a variety of antigens Cn2, Cll1, Cll2, ... changes (Thr152Ile and Ser197Gly) occurred at frameworks and 3, respectively, and the third (Tyr164Phe), occurred at CDR1 (Table 2) Antibody 6009F was expressed in Escherichia coli and the presence...

Ngày tải lên: 23/03/2014, 13:20

... interactions between mtDNA repair and degradation pathways ROS induce both single-strand and double-strand breaks in mtDNA, as well as abasic (AP) sites and base damage Both base damage and AP sites are ... protection by antioxidant defenses and DNA repair are inadequate Lack of histones in mitochondria and susceptibility of mtDNA to oxidative stress Histone proteins are reported to protect DNA from ... easily from histones to DNA, leading to DNA damage [80] In addition, damage induced by Cu2+ ⁄ H2O2 is enhanced in nucleosomal DNA compared with naked DNA [37], and some DNA peptide interactions...

Ngày tải lên: 29/03/2014, 22:21

... amount of enzyme required to digest p.g of a standard DNA in h under optimal temperature and buffer conditions The standard DNA used is normally h DNA Hence, for EcoRI (for example), there are ... [Boston, MA]) for use m random primed labeling of DNA These kits are simple and efficient This protocol 1s for purrfied DNA In most cases, the DNA can be used without purifying the DNA after preparative ... E., and Sauer, G (1983) The simultaneous extraction of highmolecular-weight DNA and of RNA from sohd tumours Anal Biochem 134, 288-294 25 Mirkes, P E (1985) Simultaneous banding of rat embryo DNA, ...

Ngày tải lên: 10/04/2014, 11:03