anti trust and regulation of public utilities
Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx
... Scientific Fund of the Ministry of Education, Science and Culture of Japan (G.T.); the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation ... of miR-221 and miR-222 in PC12 cells, and that this induction is dependent on sustained activation of the ERK1 ⁄ pathway [20] Furthermore, the induction of miR-34a depends on the activation of ... Indeed, several examples of feedback regulation that involve miRNAs have been reported Below, we attempt to summarize our recent understanding of feedback regulation of signal cascades that involve...
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Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx
... pattern and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ... promoter region of INO1 and other genes that are part of the INO1 regulon [45] As regulation of phospholipid biosynthesis and degradation are likely to be coordinated, it was of interest to examine ... both wild-type and spo14D cells Future identification of the PtdEtn-PLD gene and analysis of its promoter region will allow examination of its transcriptional regulation by inositol and the possible...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... Purification and quantification of endocannabinoids The extraction, purification and quantification of anandamide, 2-AG and PalEtn from immature and mature dendritic cells requires a set of different ... macrophages and lymphocytes are able to produce a higher amount of anandamide and/ or 2-AG [21,23–26] IgE-dependent stimulation of RBL-2H3 cells also leads to the formation of anandamide and of its ... brain and spleen were removed and placed in nitrogen Antibodies Rabbit antihuman CB1 and CB2 polyclonal antibodies and also the corresponding blocking peptides were from Cayman The CB1 antibody...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... constituent of the Tic motor complex (see below) The present minireview provides a short description of recent advances in the understanding of the channel-, motor- and regulatory components of the ... already clearly affected [13] Antisense plants of the pea ortholog and main Arabidopsis isoform of Tic20, AtTic20-I, similarly exhibit pronounced chloroplast defects, and attic20-I knockouts were ... function (e.g in ligand-gated and voltage-gated K+ channels) [16], and thus could provide an explanation for the observed channel activity of Tic110, as well as for the binding of transit peptides...
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Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt
... reporter gene (lanes 4–6) and monoclonal antiTAFII135 antibodies Lanes and 4, no antibody added to the in vitro transcription reaction; lanes and 5, lL of 300-fold diluted antibody was added to the ... R2 TATA-box, and also as a repressor of transcription, as a deletion or a mutation of this sequence or addition of TAFII135 antibodies leads to a three- to fivefold up -regulation of transcription ... lanes and 6, lL of 60-fold diluted antibody was added to the in vitro transcription reactions All bands were quantified using the Scion Image program The upper numbers represent the intensity of...
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Báo cáo khoa học: Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase ppt
... number of phosphate containing substrates, and it has a high specificity for nucleotide monophosphates and, in particular, UMP and GMP The structure of NagD has been determined and the occurrence of ... family of NagDlike phosphatases to be characterized at the level of gene regulation Results and Discussion The araL gene in the context of the B subtilis genome and in silico analysis of AraL ... Fig Overproduction and purification of recombinant AraL-His6 (A) Analysis of the soluble (S) and insoluble (P) protein fraction (20 lg of total protein) of induced cultures of E coli Bl21(DE3)...
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Characteristics and Regulation of Exempt Institutions and the Implications of Removing the Exemptions pptx
... corporation Office of the Comptroller of the Currency Office of Thrift Supervision savings and loans Securities and Exchange Commission Summary of Deposits Department of the Treasury This is a work of ... availability and allocation of credit, the stability of the financial system and the economy, and the safe and sound operations of such institutions To determine the number of certain types of financial ... other hand, accounted for about percent of credit card loans, but they accounted for less than percent of construction and land development loans and almost none of any other type of loan Grandfathered...
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Consultative Document Strengthening Oversight and Regulation of Shadow Banking docx
... Weighted-average • Outstanding amount of function 23 • Outstanding amount of off-balance sheet exposures by instruments (compared to capital) • Outstanding amount of off-balance sheet exposures ... excessive and/ or inappropriately reflected in the funding costs of the banks and mortgage companies 2.5 Securitisation and funding of financial entities Provision of funding to related-banks and/ or ... availability and hence on the real economy by expanding their businesses and facilitating the creation of credit in boom times where risk appetite is high and credit costs and losses are low, and scaling...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... detection (Pierce) of the protected probe and RNA ladder Quantitative RT-PCR Quantitative reverse transcription-PCR (qRT-PCR) was used to determine the number of copies of mature and unspliced CB1 ... (C and D) are shown For each series of protection reactions, 10 pg of undigested probe control (P) and a no target control that consists of 400 pg of probe combined with excess yeast RNA and ... rate of transcription of the CB1 gene was decreased in the striata of R6 mice and that this decrease led to the observed decrease in steady-state levels of mature CB1 mRNA Comparison of human and...
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Báo cáo khoa học: Function and regulation of ABCA1 – membrane meso-domain organization and reorganization pptx
... kinase (JAK2) and casein kinase (CK2) are involved in the regulation of ABCA1 activity and stability by apoA-I The interaction of apoA-I with ABCA1 increases the cellular cAMP content and ABCA1 phosphorylation ... Function and regulation of ABCA1 CDC42, a member of the Rho GTPase family, is reported to interact with ABCA1 and the interaction enhances apoA-I-mediated cholesterol efflux [47] Palmitoylation of ABCA1 ... 3195 Function and regulation of ABCA1 K Nagao et al ABCA1 whereas direct binding of photoactive cholesterol to ABCA1 could not be detected [74]; however, analysis of the functions of ABCA7 raised...
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Báo cáo khoa học: Signalling and regulation of collagen I synthesis by ET-1 and TGF-b1 doc
... effects of ET-1 on collagen I synthesis and breakdown clearly indicate an important role for ET-1 in the dynamic regulation of ECM and its dysregulation in disease [19] ET-1 and its isoforms ET-2 and ... expression of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and inducing that of matrix metalloproteinase-2 (MMP-2) [7,16], which in turn degrades MMP-1 cleaved collagen I [17] Regulation of ... involvement of the PC-phospholipases We used D609 to specifically inhibit PC-PLC [46], and neomycin [47–49] and N-butanol [50–52] as specific inhibitors of PLD The inhibition of PC-PLC by D609 (Fig 5B) and...
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Báo cáo khoa học: Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase pptx
... (a) Arg14 of ANP hydrogen bonds with Glu119 of ECD monomer A (Glu119A) and Asp62 of ECD monomer B (Asp62B) Arg95A and Asp62B are 1820 also hydrogen bonded, contributing to the stability of the complex; ... effects of ANP is also observed in saltdepleted rats; this occurs independent of the RAA and sympathetic systems, and is not caused by receptor downregulation [47] Although it is beyond the scope of ... natriuretic and diuretic regulation [52] Similar tubular synthesis and urinary excretion of CNP has also been reported [61] In addition to urodilatin, ANP and other natriuretic peptides of cardiac...
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Báo cáo hóa học: "Programmed cell death-1 (PD-1) at the heart of heterologous prime-boost vaccines and regulation of CD8+ T cell immunity" doc
... properties of nonreplicating vectors and biological response modifiers by direct intra-nodal administration of plasmid and peptide [19,41] We showed that the sequence and the route of administration of ... treatment of diseases such as HIV, other microbes and cancer [11,22-37] In addition, such protocols offer a more Page of 11 practical alternative for active immunotherapy of cancer and other ... diminished anti- vector antibody responses [13] known to interfere with immunity against target epitopes through the clearance and degradation of vaccine via vaccine-antibody immune complexes; and ii)...
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genetic algorithm based optimal pwm in high power synchronous machines and regulation of observed modulation error
... identification of the current harmonic model of synchronous machine and observation of modulation error In this approach, considering the equations of synchronous machine, a model consisting of input and ... (ed.) Handbook of Genetic Algorithms Van Nostrand Reinhold: New York, 1991 De Jong KA An analysis of the behavior of a class of genetic adaptive systems Ph.D dissertation, University of Michigan, ... modelling and control of electromechanical machines and machine components Chapter presents a nonlinear model for the control of a three-DOF helicopter A helicopter model and a control method of the...
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Báo cáo y học: "Expression and regulation of CCL18 in synovial fluid neutrophils of patients with rheumatoid arthritis" potx
... upregulation of CCL3, CCL18, CXCL8 and CXCL10 mRNAs and the downregulation of CCL21 mRNA Blockade of CCL18 expression by anti- TNF-α antibodies identifies CCL18 as an additional target for anti- TNF-α ... in Figure show a highly upregulated expression of CCL3 and CCL18 mRNAs, a downregulation of CCL21 mRNA (Figure 1a) and an upregulation of CXCL8 and CXCL10 mRNAs (Figure 1b) in SF PMN from each ... CXCL8 and CXCL10 and downregulation of CCL21 mRNA Materials and methods Patients SF samples were taken from knees of nine patients with active RA, for treatment and diagnostic purposes Aliquots of...
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Báo cáo y học: "Expression and regulation of neurotrophins in the nondegenerate and degenerate human intervertebral disc" pdf
... the primary antibody After washing, sections were incubated with biotinylated donkey anti- goat and goat anti- mouse secondary antibodies (Santa Cruz, CA, USA) at dilutions of 1/300 and 1/ 400, ... morphology and the structure of the extracellular matrix Up to a total of 400 cells were counted in each region of the IVD, and the number of positively stained cells was expressed as a percentage of ... for NGF and BDNF was identified in the native chondrocyte-like cells of the NP and IAF tissue and in the fibroblast-like cells of the OAF (Figures 1a to 1f and 2a to 2f) The cell bodies of pyramidal...
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Báo cáo y học: "Composition and regulation of maternal and zygotic transcriptomes reflects species-specific reproductive mode" doc
... transcript origin of synthesis (a) Genetic manipulation of D melanogaster enables quantification of the maternal and zygotic components of mRNA abundance, allowing analysis of five gene classes ... ranking of IGR length between chicken and human, and another for its fold change between mouse and human Comparison of fold changes of all-maternal one-to-one orthologs versus the set of all one-to-one ... strict-zygotic, P < 10-4 and P < 10-3; the genome-wide set of all core and metazoan genes, P < 10-21 and P < 10-72) Similarity in regulatory architecture of maternal and zygotic genes across...
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Báo cáo y học: "Alternative way to test the efficacy of swine FMD vaccines: measurement of pigs median infected dose (PID50) and regulation of live virus challenge dose" docx
... http://www.virologyj.com/content/7/1/215 Page of Table The results of the sera titers of 14 dpv and 28 dpv of vaccinated pigs by LPB-ELISA and protective effect in swine challenged with OH/99 No of pig 10 11 12 13 14 ... ml of FMDV OH/99 suckling mice passaged strain at the ear-rootneck area after 28 dpv and FMD symptoms were monitored for 10 days The results of the antibody response titrations of 14 dpv and ... such as vaccine’s antigen content, animal’s individual status, virus challenge dose and route [13-15] The LPB-ELISA assay was recognized as one of the standard methods to detect antibody The kit...
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Báo cáo y học: " Immunohistochemical detection and regulation of a5 nicotinic acetylcholine receptor (nAChR) subunits by FoxA2 during mouse lung organogenesis" pps
... Page of 11 Immunobotting and ELISAs were used to determine the specificity of the a5 antibody and it was determined to be effective with tissues embedded in paraffin [18] A rabbit polyclonal antibody ... FoxA2, a general marker of primitive respiratory and airway epithelium in the proximal and distal lung (Figure 2A, B) Co-expression of a5 and FoxA2 was also detected in proximal and distal pulmonary ... PN4 and PN10, and a return to pronounced expression at PN20 a5 expression was observed in differentiating ATI and ATII cells and proximal Clara and ciliated cells at specific time points of organ...
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Báo cáo y học: " Induction and regulation of matrix metalloproteinase-12in human airway smooth muscle cells" ppt
... were collected and analysed by the complementary computer software (Corbett Research, Australia) Relative quantitations of gene expression were calculated using standard curves and normalized ... approximately hour and destained until the gelatinolytic bands were clearly seen Gelatinolytic bands at 45kDa represent the active form of MMP-12 Relevant band intensities were quantified by scanning ... 6:148 Figure of 1β Activity MMP-12 secreted by ASMC and stimulation by ILActivity of MMP-12 secreted by ASMC and stimulation by IL1β (A) Gelatin zymography detection of activity of MMPs released...
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