angle sum of a triangle and quadrilateral

... antibodies were as follows: anti-porin from Calbiochem, anti-HA from Covance BabCo, anti-mouse and anti-rabbit secondary antibodies from Bio-Rad Laboratories and Amersham Pharmacia Biotech, respectively. Antibodies ... fraction is designated as the mitochondrial fraction. Immunochemical assays and antibodies Mitochondrial protein samples (between 50 and 100 lg) were separated by SDS/PAGE and BN/PAGE and trans- ferred ... essential subunit for assembly of an active mammalian mitochondrial complex I. Experimental procedures Cell lines and cell culture The isolation and preliminary biochemical and genetic characterization...

Ngày tải lên: 19/02/2014, 16:20

... was inhibited not only by unlabeled Bip-Pro itself, but also by well known sub- strates of H + ⁄ peptide cotransporters, such as Gly-Sar, Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala, d-aminolevulinic ... 5907 Synthesis and characterization of a new and radiolabeled high-affinity substrate for H + /peptide cotransporters Ilka Knu ¨ tter 1 , Bianka Hartrodt 2 ,Ge ´ za To ´ th 3 , Attila Keresztes 3 , Gabor ... JC, Fujita T, Liang R, Ganapathy V & Lei- bach FH (1998) Identification of a potential substrate binding domain in the mammalian peptide transporters PEPT1 and PEPT2 using PEPT1-PEPT2 and PEPT2- PEPT1...

Ngày tải lên: 07/03/2014, 05:20

... 5¢ RACE were: 5¢-GGGCATCACGGA AGAAATAG-3¢ for a reverse transcription and 5¢-GC TCTAGAGCATTCGTCACATCGATACC-3¢ with 5¢-AA GGAATT(dC)14 for the following amplification. The ther- mal profile was 40 ... DNA ladders were obtained from Promega. Factor Xa protease was purchased from Biolabs (Beverly, MA, USA). All other chemicals used were analytical grade. Plant material and nucleic acid isolation ... far-UV CD spectra of rPAC, rPBC, rPAB and native pulchellin. CD analyses for the rPAC sample showed a protein profile with predominance of a- helical elements [37]: two negative bands at 222 and 208...

Ngày tải lên: 07/03/2014, 16:20

... L., Velan, B., Lazar, A. , Kronman, C., Barak, D., Ariel, N., Shaerman, A. , Silman, I. & Sussman, J.L. (2000) Structures of r ecombinan t native and E202Q mutant h uman acetylcholinesterase ... plasma BChE showed a faint band at 170 kDa (nonreducible dimer) and a major broad band at 85 kDa (monomer) under r educing conditions (Fig. 2A) . The apparent molec- ular mass of the r ecombinant monomer ... Henchman,R.,Tai,K.,Shen,T.B.,Bo È rjesson, U., Philippopou- los, M. & Mccammon, J .A. (2001) A Structural and Dynamical Analysis of Water in Acetylcholinesterase (Paper presented at the American...

Ngày tải lên: 17/03/2014, 11:20

... unrelated to the Tat system [5]. TatE is a structural and functional homolog of TatA and encoded at a different locus [6]. TatA and TatB together can form a double-layered ring structure and are ... assembly pathways can also act in conjunction with translocation [17]. Moreover, natural Tat substrates that acquire a folded and often cofactor- containing state before their translocation appear ... m M dithiothreitol. Aggregated material was removed by a final centrifugation at 15 000 g, and the clear supernatant was divided into aliquots and frozen in liquid nitrogen. Cofactor tracing and membrane-targeting...

Ngày tải lên: 23/03/2014, 21:20

... reactivity of a- hydroxyhaem and verdohaem bound to haem oxygenase-1 to dioxygen and sodium dithionite Hiroshi Sakamoto 1 , Yoshiaki Omata 1 , Shunsuke Hayashi 1 , Saori Harada 1 , Graham Palmer 2 and ... dithionite was added gradually to the verdohaem complex under anaerobic conditions, decrea- ses in absorbance at 400, 535 and 690 nm took place and broad bands appeared at 431 and 795 nm (Fig. 4A, spectrum a) . ... first increased and then disappeared, indicates the formation and degradation of a trace amount of the CO-ferrous verdohaem produced from the free a- hydroxyhaem. Acidification and extraction of the product...

Ngày tải lên: 23/03/2014, 21:20

... words, occlusal traits that affect dental appearance and have an impact on participants' daily lives may not be captured by IOTN. In addition, DAI has many more measures of malocclusion affecting ... eruption, defects of cleft lip and pal- ate as well as any craniofacial anomaly, Class II and Class III buccal occlusions, and hypodontia. Only the highest scoring trait is used to assess treatment need ... for individuals who have a DHC grade of 4 or 5, or grade 3 with an AC of 6 or above. All other cases were therefore classified as having no need. For DAI, 10 occlusal traits were assessed and a score was...

Ngày tải lên: 18/06/2014, 19:20

... by Nagata et al. [27], who compared feasibility of health measurement response scales using four, five and seven categories and a visual analog scale. The level of missing data was least and ... data quality and the thor- ough testing of validity against other standards. The mod- erate response rate and that all data is self-reported, represent study limitations. An external, unrelated ... nina.osteras@medisin.uio.no; Pål Gulbrandsen - pal.gulbrandsen@ahus.no; Andrew Garratt - andrew.garratt@kunnskapssenteret.no; Jūratë Šaltytë Benth - jurate@ahus.no; Fredrik A Dahl - fredrik.dahl@ahus.no;...

Ngày tải lên: 18/06/2014, 22:20

... The eluted mAbs and mAb-FIs were concentrated and stored at -80°C. Analytic characterization of proteins The protein concentration of mAb and mAb-FI samples was determined by measuring the optical density ... presence and absence of a reducing agent (5 mM 1,4-dithiotreitol) and staining with Coomassie brilliant blue. The aggregate content of mAb and mAb-FI samples was analyzed by high-performance SEC ... using a TSK3000SWxl analytical size-exclusion col- umn (TosoHaas, Stuttgart, Germany). The integrity of the amino acid backbone of reduced mAb and mAb-FI light and heavy chains were verified by NanoElectrospray...

Ngày tải lên: 20/06/2014, 01:20

... Shah, WM: A generalization of a theorem of Paul Turan. J Ramanujan Math Soc. 1,67–72 (1996) 6. Aziz, A, Rather, NA: A refinement of a theorem of Paul Turan concerning polynomials. Math Ineq Appl. ... 2011:111 http://www.journalofinequalitiesandapplications.com/content/2011/1/111 Page 5 of 9 RESEARC H Open Access On the maximum modulus of a polynomial and its polar derivative Ahmad Zireh Correspondence: azireh@shahroodut.ac.ir Department ... polynomials not vanishing inside a circle. J Inequal Pure Appl Math. 6,1–9 (2005) Zireh Journal of Inequalities and Applications 2011, 2011:111 http://www.journalofinequalitiesandapplications.com/content/2011/1/111 Page...

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