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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... antibodieswere as follows: anti-porin from Calbiochem, anti-HA fromCovance BabCo, anti-mouse and anti-rabbit secondaryantibodies from Bio-Rad Laboratories and AmershamPharmacia Biotech, respectively. Antibodies ... fraction is designated as themitochondrial fraction.Immunochemical assays and antibodiesMitochondrial protein samples (between 50 and 100 lg)were separated by SDS/PAGE and BN/PAGE and trans-ferred ... essentialsubunit for assembly of an active mammalian mitochondrialcomplex I.Experimental proceduresCell lines and cell cultureThe isolation and preliminary biochemical and geneticcharacterization...
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Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... was inhibited not only byunlabeled Bip-Pro itself, but also by well known sub-strates of H+⁄ peptide cotransporters, such as Gly-Sar,Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,d-aminolevulinic ... 5907Synthesis and characterization of a new and radiolabeledhigh-affinity substrate for H+/peptide cotransportersIlka Knu¨tter1, Bianka Hartrodt2,Ge´za To´th3, Attila Keresztes3, Gabor ... JC, Fujita T, Liang R, Ganapathy V & Lei-bach FH (1998) Identification of a potential substratebinding domain in the mammalian peptide transportersPEPT1 and PEPT2 using PEPT1-PEPT2 and PEPT2-PEPT1...
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Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt
... 5¢ RACE were: 5¢-GGGCATCACGGAAGAAATAG-3¢ for a reverse transcription and 5¢-GCTCTAGAGCATTCGTCACATCGATACC-3¢ with 5¢-AAGGAATT(dC)14 for the following amplification. The ther-mal profile was 40 ... DNA ladders were obtained from Promega.Factor Xa protease was purchased from Biolabs (Beverly,MA, USA). All other chemicals used were analytical grade.Plant material and nucleic acid isolation ... far-UV CD spectra of rPAC, rPBC, rPAB and native pulchellin. CD analyses for the rPACsample showed a protein profile with predominance of a- helical elements [37]: two negative bands at 222 and 208...
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Báo cáo khoa học: Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase pdf
... L., Velan, B., Lazar, A. , Kronman, C., Barak, D., Ariel, N., Shaerman, A. ,Silman, I. & Sussman, J.L. (2000) Structures of r ecombinan tnative and E202Q mutant h uman acetylcholinesterase ... plasmaBChE showed a faint band at 170 kDa (nonreducibledimer) and a major broad band at 85 kDa (monomer)under r educing conditions (Fig. 2A) . The apparent molec-ular mass of the r ecombinant monomer ... Henchman,R.,Tai,K.,Shen,T.B.,BoÈrjesson, U., Philippopou-los, M. & Mccammon, J .A. (2001) A Structural and DynamicalAnalysis of Water in Acetylcholinesterase (Paper presented at theAmerican...
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Báo cáo khoa học: Membrane targeting of a folded and cofactor-containing protein potx
... unrelated tothe Tat system [5]. TatE is a structural and functionalhomolog of TatA and encoded at a different locus [6]. TatA and TatB together can form a double-layered ring structure and are ... assembly pathways can also act inconjunction with translocation [17]. Moreover, naturalTat substrates that acquire a folded and often cofactor-containing state before their translocation appear ... mMdithiothreitol. Aggregatedmaterial was removed by a final centrifugation at 15 000 g, and the clear supernatant was divided into aliquots and frozen in liquid nitrogen.Cofactor tracing and membrane-targeting...
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Báo cáo Y học: The reactivity of a-hydroxyhaem and verdohaem bound to haem oxygenase-1 to dioxygen and sodium dithionite pdf
... reactivity of a- hydroxyhaem and verdohaem boundto haem oxygenase-1 to dioxygen and sodium dithioniteHiroshi Sakamoto1, Yoshiaki Omata1, Shunsuke Hayashi1, Saori Harada1, Graham Palmer2 and ... dithionite was added gradually tothe verdohaem complex under anaerobic conditions, decrea-ses in absorbance at 400, 535 and 690 nm took place and broad bands appeared at 431 and 795 nm (Fig. 4A, spectrum a) . ... first increased and then disappeared, indicates the formation and degradation of a trace amount of the CO-ferrous verdohaem produced fromthe free a- hydroxyhaem. Acidification and extraction of theproduct...
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báo cáo hóa học: " Comparison of the discriminative ability of a generic and a condition-specific OHRQoL measure in adolescents with and without normative need for orthodontic treatment" potx
... words,occlusal traits that affect dental appearance and have animpact on participants' daily lives may not be captured byIOTN. In addition, DAI has many more measures of malocclusion affecting ... eruption, defects of cleft lip and pal-ate as well as any craniofacial anomaly, Class II and ClassIII buccal occlusions, and hypodontia. Only the highestscoring trait is used to assess treatment need ... forindividuals who have a DHC grade of 4 or 5, or grade 3with an AC of 6 or above. All other cases were thereforeclassified as having no need. For DAI, 10 occlusal traitswere assessed and a score was...
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báo cáo hóa học: " A randomised comparison of a four- and a five-point scale version of the Norwegian Function Assessment Scale" doc
... byNagata et al. [27], who compared feasibility of healthmeasurement response scales using four, five and sevencategories and a visual analog scale. The level of missingdata was least and ... data quality and the thor-ough testing of validity against other standards. The mod-erate response rate and that all data is self-reported,represent study limitations. An external, unrelated ... nina.osteras@medisin.uio.no; Pål Gulbrandsen - pal.gulbrandsen@ahus.no; Andrew Garratt - andrew.garratt@kunnskapssenteret.no; Jūratë Šaltytë Benth - jurate@ahus.no; Fredrik A Dahl - fredrik.dahl@ahus.no;...
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Báo cáo hóa học: " Closing two doors of viral entry: Intramolecular combination of a coreceptor- and fusion inhibitor of HIV-1" docx
... Theeluted mAbs and mAb-FIs were concentrated and stored at-80°C.Analytic characterization of proteinsThe protein concentration of mAb and mAb-FI sampleswas determined by measuring the optical density ... presence and absence of a reducing agent (5 mM 1,4-dithiotreitol) and staining withCoomassie brilliant blue. The aggregate content of mAb and mAb-FI samples was analyzed by high-performanceSEC ... using a TSK3000SWxl analytical size-exclusion col-umn (TosoHaas, Stuttgart, Germany). The integrity of theamino acid backbone of reduced mAb and mAb-FI light and heavy chains were verified by NanoElectrospray...
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Báo cáo hóa học: " On the maximum modulus of a polynomial and its polar derivative" potx
... Shah, WM: A generalization of a theorem of Paul Turan. J Ramanujan Math Soc. 1,67–72 (1996)6. Aziz, A, Rather, NA: A refinement of a theorem of Paul Turan concerning polynomials. Math Ineq Appl. ... 2011:111http://www.journalofinequalitiesandapplications.com/content/2011/1/111Page 5 of 9RESEARC H Open AccessOn the maximum modulus of a polynomial and its polar derivativeAhmad ZirehCorrespondence:azireh@shahroodut.ac.irDepartment ... polynomials not vanishing inside a circle. J Inequal Pure ApplMath. 6,1–9 (2005)Zireh Journal of Inequalities and Applications 2011, 2011:111http://www.journalofinequalitiesandapplications.com/content/2011/1/111Page...
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