angiogenesis inhibition induces cell death

... intracranial cancer cells, by either inducing cell death or preventing further proliferation; b) to explore the molecular mechanism of cell death by Ruta + Ca3(PO4)2 treatment of brain cancer cells in vitro; ... indicates that Ruta induces cell death in a dose- and duration-dependent manner in human MGR1 brain cancer cells, followed by saturation effects However, Ruta protects B-lymphoid cells and PHAstimulated ... may result in the cancer cells becoming more sensitive to cell death than normal cells (23) The Ruta and Ca3(PO4)2 combination was capable of protecting normal B-lymphoid cells against H2O2-induced...

Ngày tải lên: 22/03/2014, 17:20

... μg/ml), cells were visualized using a microscope (1000 ×, Olympus) Cell growth assay Cell proliferation was assessed by MTT assay NB4-R2 cells were plated in 96-well plates at 2.5 × 104 cells/ml ... VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in vitro NB4-R2 cells were treated with VX-680 at ... the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells were incubated with increasing doses of VX-680 (1, 2, and 10 nM) for 24 hr and 48 hr (A) Cell viability was measured...

Ngày tải lên: 18/06/2014, 19:20

... μg/ml), cells were visualized using a microscope (1000 ×, Olympus) Cell growth assay Cell proliferation was assessed by MTT assay NB4-R2 cells were plated in 96-well plates at 2.5 × 104 cells/ml ... VX-680 suppresses cell growth and induces cell apoptosis in NB4-R2 cells Next, we studied if VX-680 could suppress proliferation in NB4-R2 cells in vitro NB4-R2 cells were treated with VX-680 at ... the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells were incubated with increasing doses of VX-680 (1, 2, and 10 nM) for 24 hr and 48 hr (A) Cell viability was measured...

Ngày tải lên: 20/06/2014, 03:20

... vitro Brucella suis infection prevents the programmed cell death of human monocytic cells Infect Immun 2000, 68:342-351 Salvesen GS, Dixit VM: Caspases: intracellular signaling by proteolysis Cell ... (Fig 11) in AA100jm-infected cells Discussion Cell death is typically discussed as necrosis, apoptosis, or pyroptosis While necrosis is characterized as accidental cell death due to physical damage, ... cause host cell death with features of apoptosis [22-24] Pyroptosis is a recently described type of cell death, in which caspase is activated and inflammatory cytokines are released as cells are...

Ngày tải lên: 12/08/2014, 15:21

... and bovine seminal RNase [2] Mode of cell death induced by RNase T1/PS/HVJ Anticancer drugs induce cell death via apoptosis or necrosis Because necrotic cell death could produce severe inflammatory ... decreased the cell number slightly, the shape of the cells was indistinguishable from that of untreated cells and different from that of the cells treated with RNase T1/HVJ In the cell culture ... z-VAD-fmk significantly protected cells from RNase T1/PS/HVJ-induced cell death (Fig 5) These results suggest that RNase T1/PS/HVJ can induce apoptosis in K-562 cells The K-562 cell line is derived from...

Ngày tải lên: 23/03/2014, 12:20

... ANT1-silencing induces glioblastoma cell death A Lena et al Because of their multitask nature, ANTs are involved in several aspects of cell metabolism, as well as in cell survival ⁄ death processes, ... on ADF cell viability, indicating that the reduction in cell viability induced by ANT1 siRNA requires protein synthesis ANT1-silencing induces ADF cell apoptosis and a non-apoptotic cell death ... 2010 FEBS 2857 ANT1-silencing induces glioblastoma cell death A Lena et al A B C D E G F * H I J Fig Analysis of cell death modality in ANT1 siRNA-transfected ADF cells by transmission electron...

Ngày tải lên: 29/03/2014, 09:20

... may be sufficient to overcome the cellular death threshold and to induce apoptotic death of LNCaP cells [16,36] In PC3-cells, ionizing radiation also decreased cellular Bcl-2 levels but ErPC3 did ... pathway Because inhibition of Akt can lower the threshold for cell death induction, we next examined whether an inhibition of the Akt survival pathway by ErPC3 sensitizes the cells to the cytotoxic ... proliferation, cell death induction, or both Therefore, in a next step, we examined whether the anti-neoplastic effects of ErPC3 and ionizing radiation include induction of cell death, in particular...

Ngày tải lên: 09/08/2014, 09:20

... silibinin-induced cell death in human glioma cells Role of calpain and protein kinase C (PKC) activation in ROS generation and cell death induced by silibinin The silibinin-induced cell death was associated ... effectively prevented the silibinin-induced cell death (Figure 5D) These data suggest that calpain activation induces AIF-dependent cell death in silibinin-treated cells This is the first report showing ... downstream of calpain in the silibinin-induced cell death Similar results are reported in human U-937 leukemia cells in which the flavonoid wogonin induces cell arrest through PKCδ activation [18]...

Ngày tải lên: 10/08/2014, 10:21

... cytosolic cell extracts of × 104 cells were performed as described [39] Microscopy To study morphological alterations of the cell lines, microscopic analysis were performed Therefore, × 104 cells/well ... time D): × 10 ence (A, or different × 104, and to 104 cells/well (C,UC cells (A, celland their induction forapoptotic nuclei (C,in the pres-6 cells or B) of of tetracycline (Tet) in the UHCV and ... HCV-proteins in the UHCV and UC cells (A, B) and their induction of apoptotic nuclei (C, D): × 106 cells (A, B) or × 104, × 104, and × 104 cells/well (C, D) of each cell line were cultured for the...

Ngày tải lên: 12/08/2014, 04:21

... liver cells, including hepatocytes [76,77], stellate cells [78], sinusoids endothelial cells [79], kupffer cells, billary epithelial cells [80], as well as immune cells such as NK cell, NKT cells ... DsRNA triggers cell death in HCC cells that express TLR3 52 3.6 DsRNA induced tumour cell death is mediated by TLR3 56 3.7 Activated NK cells increases with dsRNA stimulation 60 3.8 NK cells have ... remaining cells are endothelial cells, stellate cells, Kupffer cells and lymphocytes The immune cell comprises mainly phagocytic cells such as neutrophils and macrophages, lymphocytic cells such...

Ngày tải lên: 13/10/2015, 16:50

... cause cell death during the period tested (24 h posttransfection; Fig 4) These results indicate that transient expression of the minor structural proteins in permissive cells induces cell death; ... VP2–EGFP and VP3–EGFP with cellular substructures Cells expressing EGFP only were used as a control EM pictures of cells at early timepoints post-transfection, but before cell death, were obtained ... and ⁄ or with other intracellular membranes causing their damage Cell death induced by VP2 and VP3 is dependent on the activation of caspases To dissect whether the cell death induced by VP2 or...

Ngày tải lên: 16/02/2014, 09:20

... and added to the cells After 24, 48 and 72 h, CM were collected and the cells were processed further (total RNA and ⁄ or cell lysates) Cell survival ⁄ proliferation assay The cells were seeded ... and cell lysates (CM and L, respectively) were collected and TSP1 was detected by western blotting Note the low TSP1 secretion and higher intracellular levels in RCC cells (F) HNK and P769 cells ... and RCC cell lines differed in their ability to induce angiogenesis Conditioned media (CM) from P769 and other RCC cell lines were potently angiogenic in rat and mouse corneal assay for angiogenesis...

Ngày tải lên: 07/03/2014, 05:20

... apoptotic cell death induced by ionizing radiation in human cervical cancer cells, we treated HeLa cells with 10 Gy radiation, and analyzed induction of apoptotic cell death by fluorescence activated cell ... cell death M.-J Kim et al Fig Ionizing radiation induces expression of Fas and activation of caspases in human cervical cancer cells (A) Ionizing radiation-induced apoptotic cell death HeLa cells ... apoptotic cell death in human cervical cancer cells Inhibition of JNK effectively protected cells from radiation-induced loss of mitochondrial membrane potential and apoptotic cell death FEBS...

Ngày tải lên: 07/03/2014, 05:20

... effector of cell death Cell Death Diff 6, 516–524 Mateo, V., Lagneaux, L., Bron, D., Biron, G., Armant, M., Delespesse, G & Sarfati, M (1999) CD47 ligation induces caspase-independent cell death in ... autophagic cell death upon treatment with tamoxifen More recent work showed that in apoptotic cell death induced by tyrphostin A25 in the human colon cancer cell line HT29/HI1, early stages of the death ... autophagic cell death is a separate form of programmed cell death that is distinctly different from apoptosis In keeping with this interpretation, intensive irradiation led to up to 30% cell death...

Ngày tải lên: 07/03/2014, 15:20

... Mechanisms of steroidtriggered programmed cell death in Drosophila Semin Cell Dev Biol 16, 237–243 Fahrbach SE, Nambu JR & Schwartz LM (2005) Programmed cell death in insect neuromuscular systems ... an insect steroid hormone in steroid-induced programmed cell death Mol Cell Endocrinol 263, 18–28 Kakei M, Iwami M & Sakurai S (2005) Death commitment in the anterior silk gland of the silkworm, ... toxic factor for cells, NADPH oxidase (NOX) in mammalian cells generates reactive oxygen species, including hydrogen peroxide, that play an important role in the regulation of cell proliferation...

Ngày tải lên: 15/03/2014, 00:20

... of cells in each phase of the cell cycle as evaluated by flow cytometry Percentage of cells in the phases of the cellcycle G1 C33A CASKI HeLa DHEA induces apoptotic and necrotic death G2 ⁄ M Cell ... has been associated with an arrest of the cell cycle and cell death in BV-2 cells, a murine microglial cell line, in hepatoma cell lines and in HepG2 cells [25,54,55] Our results showed that pharmacological ... cells such as T lymphocytes, isolated neurons and endothelial cells, or malignant cell lines such as human hepatoblastoma cells (HepG2), colon adenocarcinoma cells (HT-29) and breast cancer cells...

Ngày tải lên: 16/03/2014, 00:20

... promote cell death Tumour cell death arising from such drug treatments requires BIM to varying degrees; in many cases BIM acts in concert with other BOPs, such as BAD Despite this, cell death in ... BIM-dependent cell death in tumour cells Here we review recent advances in understanding BIM regulation and analyze the results of studies which suggest that BIM is an important mediator of tumour cell death ... promoting aberrant cell survival in tumours that harbour mutations in oncogenes which control these pathways and (b) the role of BIM in tumour cell death arising from targeted inhibition of such...

Ngày tải lên: 16/03/2014, 00:20

... for cell death propagation ⁄ manifestation, whereas OPDA would counteract the establishment of the cell death phenotype (see below) Wagner et al [30] and Kim et al [31] demonstrated that cell death ... stages preceding or correlating with cell death Implication of JA in cell death regulation Plant hormones such as ethylene, SA and JA play important roles in cell death regulation This is illustrated ... Reinbothe et al JA and cell death Fig Central role of JA in cell death regulation in plants Animals respond to many external factors with a plethora of different cell death pathways of which...

Ngày tải lên: 16/03/2014, 02:20

... analysis showing the intracellular distribution of CD47 molecule in zymosan-treated cells (central panel) and OPZ-treated cells (right panel) (E) DIC (Nomarski) micrographs showing cell aggregates in ... proteins, nor cell death On this basis, we can also hypothesize that, in some immunopathological instances, the increased number of platelets could be due to a defective death of these cells (although ... percentages of annexin V-positive cells (bottom right quadrant) or annexin V ⁄ trypan blue double positive cells (upper quadrant) Note the very low percentage of cells that are positive for trypan...

Ngày tải lên: 16/03/2014, 04:20

... cell density We thus estimated the number of falsepositive colonies recovered on this medium by mixing various proportions of the V148T chitosanase-expressing cells and chitosanase-negative cells ... [22,23] The extent of growth inhibition is dependent on chitosan concentration, average molecular mass, the pH of the medium and salt composition We noticed that growth inhibition could be suppressed ... oligosaccharides that can be transported inside the cell to be used as C and N source, chitosanase could also play the role of a stress enzyme, protecting the microbial cells against chitosan This possibility...

Ngày tải lên: 16/03/2014, 04:20