and rhcmv specific t cell responses in peripheral blood

... 5′GTCTCTCTGGTTAGACCAG-3′, Primer C 5′-CTAGTCAAAATTTTTGGCGTACTC-3′ and primer A and sj4.7A 5′- TTGGGAGGTGGGTTGCTTTGATAGAG-3 for spliced Kb transcript For GAPDH forward 5′ CTCTGCTCCTCCTGTTCGAC 3′ and ... RREcontaining RNA Further experiments are needed to answer the mechanistic details of how Matrin and Rev cooperate in their interactions with RRE-containing RNA One intriguing finding is that Matrin ... RNAs, these transcripts contain cis-inhibitory RNA elements which restrict their export from the nucleus into the cytoplasm in the absence of Rev binding to the RRE motif The binding of Rev to the...

Ngày tải lên: 13/08/2014, 01:21

... single chain containing an extended C-terminal domain with unknown function Although type and type RIPs are equally effective inhibitors of protein synthesis in cell extracts, the absence of the ... collected diffraction data and the resulting refinement of the structure are shown in Table A thin section of the structure with its electron-density map is shown in Fig A total of 232 out of 257 ... significant homology with various RIPs Although charybdin inhibited the rabbit reticulocyte translation system, the estimated IC50 of 27 nm indicates that it is not such a strong inhibitor of protein...

Ngày tải lên: 16/03/2014, 14:20

... contributes to the new understanding of place marketing and place branding as strategic tools in place marketing, and to strategic management literature by translating marketing concepts to match ... case-study-based investigations into destination branding especially those evaluating the long-term success of such activities Opinions in the literature are emerging at the same time that branding ... a natural starting point for place marketing, as it forces a place to determine the essential contents of marketing 17 If the city decides what it wants its brand to be, then it will try to make...

Ngày tải lên: 23/03/2014, 04:21

... of the oligoglucoside substrate and there is no interaction between subsites; secondly, the subsite affinities are additive The important thing is regularity of a value for the intrinsic rate ... Growth conditions The T viride AZ36 from Petersburg Nuclear Physics Institute strain collection was grown at 30 ^ 8C for 72 h in a 10-L fermentor with constant stirring The growth medium contained ... rate constant Kint Accordingly, the substrate binding affinity becomes the sum of the affinities for glucose residues of the substrate accessible for binding; the distinction of Vmax (kcat) values...

Ngày tải lên: 24/03/2014, 04:21

... heritability of reproductive date) and a trait determining adaptation to habitat type (here habitat type is the selective environment on a given date) One difference may lie in the continuous nature ... constraints, direct environmental influences, adaptive tactics, and adaptation to particular times Disentangling this complexity, must await the demonstration that each mechanism can work on its ... likely to mate with each other than will those reproducing at different times (i.e temporal assortative mating) If some of this timing variation is heritable, the probability that two individuals...

Ngày tải lên: 28/03/2014, 16:20

... the branches on the stem The tree bole was cut at 0.30 m and at 1m intervals thereafter up to the base of the live crown (i.e the point where the main stem bifurcated), and the part of the stem ... reviewed and compared the log-transformed linear model with the simple nonlinear form and pointed out that the latter model resulted in better fit for two datasets of Betula alleghaniensis Britton and ... elastic similarity models and Niklas [17] reported that, for very old dicot trees, H µ D0.474 implying that mature trees taper so as to maintain a constant elasticity throughout the tree In this...

Ngày tải lên: 08/08/2014, 01:21

... instruments We therefore optimized the reaction conditions on the PCR machine with the fastest heating and cooling rate - the machine that performed most poorly with the standard protocol We extended ... of biotin-adapter-ligated DNA fragments by streptavidin capture Non-phosporylated biotinylated Illumina adapters were prepared by annealing 5’-ACACTCTTTCCCTACACGACGCTCTTCCGATCxT and 5’-GATCGGAAGAGC ... polymerase and short initial (30 s) and in- cycle (10 s) denaturation times was performed on three different thermocyclers at their respective default temperature ramp settings Heating and cooling rates...

Ngày tải lên: 09/08/2014, 22:23

... acids and double dots represent conserved substitution Subtypes were determined using Viral Genotyping Tool, REGA Subtyping Tool and RIP 3.0 with maximum blast identity and their CD4 count varied ... (A81) to 15 (ND5 (data not shown) This is important because in some instances hypo-glycosylated forms of envelope have been associated with better transmission and in their ability to interact with ... Competing interests The authors declare that they have no competing interests Authors' contributions UN, VS, NG and AC carried out the experiments SD, VGR, VKS, AW were responsible for providing the...

Ngày tải lên: 10/08/2014, 05:21

... cells and not to normal cells including normal CD34+ cells Cytochemistry studies showed that after surface binding, these two peptides were internalized within minutes Intracellular localization ... AML cells HP-A2 is internalized after binding to AML cells AML cells were incubated with biotinylated-peptide HP-A2 (1 μM) at various time points The bound peptides were initially detected at the ... concomitant cell cycle arrest Studies with other differentiating agents show accumulation of cells during Table 5: Clinical characteristics of AML patients at the time the bone marrow was obtained and...

Ngày tải lên: 10/08/2014, 22:20

... (AATTGGTCAATTCCTAAAACACCATG, AAATTATGGGTTTTAAGGGCTAGAGTTC) and PGB04 (AACAAATTTACTCATTTA CCCGTGA, CCCATCAAAATCCATGCCCAAG, TTCCAAGTTCTTGTGGGAGGAG, GACTGATTTTCTCTCCACCAAGCAAG) Sequence analysis Repetitive DNA was identified ... PG29 gDNA The primer sequences (shown in 5'-3' orientation) are CTTTCAAGCCCAATACCCAAAGGCACTG and GGGAATGGCAATCACTGCATTGGTATAG for CYP720B4; and GGAGAATTAGTGAGTCATGTCGATG and CTCTGTCTGATTGGTGGAACAGGC ... 5'-UTR of the 3CAR gene on PGB02 contains a unique and conserved repeated sequence of 44 bp (TCAGGTTCTGCCATTGCCTTTTTAGTTCATTATCTTGAGCTGCC) which is located four times (with no more than two nucleotide...

Ngày tải lên: 12/08/2014, 03:21

... T- KWT-43 X65259 X75669 TGTCCTGGTTATCGCTGGACGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTG 420 -T T -A KWT-43 X65259 X75669 CTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGCCCGCTTGTCCT ... remaining 13 strains were identified as mixed genotype A and D strains by the other two genotyping methods The data showed that the KWT-43 X65259 X75669 CCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTGTGT ... CTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTATCAAGGTATGTTGCCCGCTTGTCCT 480 T -A T- G -T - KWT-43 X65259 X75669 CTAATTCCAGGATCTTCAACAACCAGCACGGGACCATGCAGAACCTGCACGACTCCTGCT...

Ngày tải lên: 12/08/2014, 04:20

... implementing a strategy that linked the rapid test to pre-emptive isolation and cohorting of MRSA patients Competing interests JS and PF are the developers and patent holders of the rapid MRSA test ... times (TATs) of the conventional method by optimizing laboratory organization and selecting faster methods Median TATs (time interval between sample delivery to the laboratory and report of the ... pre-emptive isolation and introduction of the qMRSA test to the surgical ICU; initiation of qMRSA testing and pre-emptive isolation in the medical ICU) was the primary independent variable The monthly number...

Ngày tải lên: 12/08/2014, 23:21

... window to span single digit concentrations within the identified range Cell uptake studies Uptake studies were conducted by treating cells at a density of 106 cells/cm2 in a 35 mm glass-bottom ... 50% ethanol (2x) and PBS pH 7.2 at °C Immediately after washing, the subcellularly-fractionated lysate containing mg/mL in net protein was added and the resin was shaken for h at °C One resin was ... at pH 7.2 containing 10 mM MgCl2 for h at °C The resin was harvested and washed three times with equal volumes of in PBS at pH 7.2 containing 10 mM MgCl2 The protein concentration on each resin...

Ngày tải lên: 10/09/2015, 15:48

... 278.0 HO O I 316 Appendix OTs 3-2 C22H19IO7S2 586.4 TsO O I FT-IR Data 317 OBn 3-3 C13H12O 184.2 318 Appendix TsO O OTs 3-24 C57H48O15S4 1101.2 OBn OTs O OTs FT-IR Data 319 3-5 C29H24O7 484.5 ... 544.4 TsO I FT-IR Data 329 TsO OTs OBn 5-19 C53H44O13S4 1017.2 OTs OTs 330 Appendix MeO O O 3-40 C19H14O5 322.3 O OMe FT-IR Data 331 HO O O 5-23 C17H10O5 294.3 O OH 332 Appendix TsO OTs 3-56 C30H34O7S2Si ... C30H34O7S2Si 598.8 O TES FT-IR Data 333 HO OH 3-57 C16H22O3Si 290.4 O TES 334 Appendix HO O 3-35 C16H22O3Si 290.4 O TES FT-IR Data 335 HO O 3-34 C10H7IO3 302.1 O I 336 Appendix FT-IR Data 337 HO O 5-28...

Ngày tải lên: 10/09/2015, 15:49

... Appendix Low-Resolution-Mass Data 3-2 C22H19IO7S2 586.4 TsO O OTs I 347 OBn 3-3 C13H12O 184.2 348 Appendix TsO O OTs 3-24 C57H48O15S4 1101.2 OBn OTs O OTs Low-Resolution-Mass Data 349 HO O OH 3-4 ... O O O O 362 Appendix Low-Resolution-Mass Data OTs 5-18 C20H17IO6S2 544.4 TsO I 363 TsO OTs OBn 5-19 C53H44O13S4 1017.2 OTs OTs 364 Appendix Low-Resolution-Mass Data MeO O 3-40 C19H14O5 322.3 O ... 366 Appendix TsO OTs 3-56 C30H34O7S2Si 598.8 O TES Low-Resolution-Mass Data 367 HO OH 3-57 C16H22O3Si 290.4 O TES 368 Appendix Low-Resolution-Mass Data HO O 3-35 C16H22O3Si 290.4 O TES 369 HO O...

Ngày tải lên: 10/09/2015, 15:50

... High-Resolution-Mass Data 381 O TsO I OTs 3-2 C22H19IO7S2 585.9617 382 Appendix OBn 3-3 C13H12O 184.0888 TsO O OTs 3-24 C57H48O15S4 1100.1876 OBn OTs O OTs High-Resolution-Mass Data 383 HO O ... C44H32O8 688.2097 O O High-Resolution-Mass Data 399 TsO I OTs 5-18 C20H17IO6S2 543.9511 400 Appendix TsO OTs OTs OTs OBn 5-19 C53H44O13S4 1016.1665 High-Resolution-Mass Data 401 MeO OMe O O O 3-40 C19H14O5 ... C16H22O3Si 290.1338 TES High-Resolution-Mass Data 407 O HO TES O 3-35 C16H22O3Si 290.1338 408 Appendix O HO I O 3-34 C10H7IO3 301.9440 High-Resolution-Mass Data 409 H O O O TsO OTs H 3-61 C36H28O10S2...

Ngày tải lên: 10/09/2015, 15:50

... • Affinity-Guided Isolation, Structure Elucidation and Total Synthesis of Laetirobin A and Its Analogue Synthesis for Therapeutic Development Distance Teaching University, Hagen, Germany 2005 ... results PROFESSIONAL EXPERIENCE Novartis Institute for Tropical Diseases, Singapore 2011 - present Research Investigator – Chemistry Development of anti-malaria therapeutics Boehringer Ingelheim, ... M J Discovery of Laetirobin as a Late-Stage Mitotic Blocker that Induces Programmed Cell Death (poster presentation) th 57 Natural Products Gordon Research Conference, Tilton, NH, USA, 2008 Lear...

Ngày tải lên: 10/09/2015, 15:50

... GTGGCGAATACTGGCGAGACT CCCCATTCTTTTTCACCGTCG ACGATGTGGTTTATTCTGGA CTTCACGTGACCATACATAT GCGCTGTCGAGTTCTATCGAGC CAACGGTGACTTTATCGCCATTCC CCGCAGCCAA GCGATCCCCA Expected size Reference 614 bp Fagan et al ... motility test and agglutination test with O157 and flagellar H7 antiserum (Difco, USA) For motility test, overnight cultured colonies were inoculated into motility test medium (Difco, USA) and incubated ... different detection methods and to define the molecular characteristics of the isolates using multiplex PCR and RAPD The conventional culture method showed the lowest detection rate It might be attributable...

Ngày tải lên: 07/08/2014, 18:21

... phage vector containing the bovine gene [12] and which serves as a reference for the establishment of the Texas nomenclature [14] For RB1, heterologous primers: RB1F: CTTGTGTGATTAACTTATTTAGAG and ... proposed together with their diagrammatic representations In the following years, some confusion in the bovine nomenclature led Popescu et al [14] to dene the Texas standard nomenclature during the third ... M Gautier et al several banding techniques have been applied to cattle chromosomes, resulting in different systems of cytogenetic nomenclature The rst international nomenclature of the bovine...

Ngày tải lên: 09/08/2014, 18:21

... TCTCATCTCGATCCCCAAAG; PEAMT-For5'TTGCCCTTGAG CGTTCTATT, Rev5'TACCTCCTGGCTTCAACCAT; P5 CSFor5'GATGTTTTTGCTGCCATTGA, Rev5' GC TAATC CC AACCTCAGCAC; DnaJ-For5'GGAATACAGGAGGGG GA CAT, Rev5'CCTTTTGGGAGAACCAAACA; ... strength Hoagland's solution containing 5.75 g NaCl was poured into the treatment pots, raising their final NaCl treatment concentration (in 250 ml treatment volume) to 425 mM It was determined ... BADH-For5' TGGAAAATTGCTCCAGCTCT, Rev5'CTGGACCTAATCCC GTCAAA; Actin-For5'AAACCACAAGCCCCTAAACC, Rev5 'TTGCATCACTCAGCACCTTC The PCR reaction conditions were also set as per the instruction manual in the...

Ngày tải lên: 12/08/2014, 03:20