and retama monosperma l extracts in human cervical cancer cells
Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
... medium supplemented with 10% fetal bovine serum All media were supplemented with 100 unitsÆmL)1 penicillin and 100 lgÆmL)1 streptomycin, and all cells were incubated at 37 °C in 5% CO2 Cells were ... Diego, CA, USA) Cell culture and transfection The human cervical carcinoma cell line (HeLa) was obtained from the American Type Culture Collection (Rockville, MD, USA) Cells were grown in RPMI-1640 ... findings, we found that JNK plays an important role in radiation-induced apoptotic cell death in human cervical cancer cells Inhibition of JNK effectively protected cells from radiation-induced loss...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
... silver nanoparticles and silver nanocrystals are able to reduce inflammation by altering the levels of cytokines involved in the wound-healing process [37,38] Decreased levels of IL-10 and IL-6 ... National Institute of Allergies and Infectious Diseases and the National Institute of Health and Collaborators: MT-2 (from Dr Douglas Richman), H9+ cells (from Dr Robert Gallo), HeLa-CD4-LTR-β-gal cells ... [14] following vaginal or rectal administration [15], should cause minimal or no genital symptoms following long-term repeated usage [16], should act rapidly and should offer long-lasting protection...
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Báo cáo khoa học: "Cell death by the quinoxaline dioxide DCQ in human colon cancer cells is enhanced under hypoxia and is independent of p53 and p21" pdf
... carcinoma cells and Lewis Lung Carcinoma (LLC) cells [4,8] We also showed that DCQ inhibited cell proliferation and induced apoptosis in colon T-84 cancer cell lines under normoxia via the inhibition ... cell lines except in p53+/+ cells exposed to hypoxia (Figure 6A); p21 protein also increased in all cell lines except in p53+/+ cells exposed to normoxia (Figure 6A) Exposure of the p53+/+ cells ... percentages of cells in various cell cycle phases Pre-G1 cells with DNA content < 2n represent apoptotic or necrotic cells Hoechst staining Cells were plated in 6-well plates at 1.2 × 105 and treated...
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Báo cáo khoa học: " Radiosensitization by 2-benzoyl-3-phenyl-6,7-dichloroquinoxaline 1,4-dioxide under oxia and hypoxia in human colon cancer cells" pdf
... FHs74Int normal intestinal cell line After establishing effects of DCQ and IR in cancer cells, we compared DCQ efficacy in normal cells For this purpose, FHs74Int normal human intestinal cells were ... ng/ml epidermal growth factor Human colon cancer cell lines (DLD1, HT-29, HCT-116, and SW-480) were grown in RPMI 1640 containing L- Glutamine and 25 mm HEPES All Cell cycle analysis using flow ... apoptotic cells in treated samples as compared to the control samples Single Cell Gel Electrophoresis (SCGE)/comet assay DNA damage, including single strand breaks (SSB) and alkali labile sites (ALS),...
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báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP" pptx
... of directly proportional to the number of living cells In this study, HCT116 cells were seeded on 96-well plates at a density of × 103 cells/ well and allowed to adhere overnight The cells were ... and β-actin proteins β-actin was used as a cell internal protein marker The control groups included untreated cells (CTL-NT) and cells treated with 0.15 μg/ml H2O2 only DCPIP NPs significantly ... HCT116/IκB-Luc cells were seeded on 96-well plates at a density of × 104 cells/ well and allowed to adhere overnight to the plastic substrate The cells were then treated with increasing (2fold) concentrations...
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Experimentation, modeling and control of calcium dynamics in human vascular endothelial cells
... Geiger et al (Geiger et al [1992]) obtained similar results and analyzed the spatial/temporal calcium dynamics from a single VEC Helmlinger et al (Helmlinger et al [1995]) applied pulsatile and steady ... biological/physiological and engineering perspectives Proficient skills in handling cells and chip In our case, we need to integrate cell culture, ATP release measurement and Ca2+ imaging into the one system The main ... reservoir and should be properly set since a too small value will lead to inadequate gas and nutrients exchange while a too large value will cause the remodeling of endothelial cells As to the...
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EXPRESSION ANALYSIS AND FUNCTIONAL STUDY OF HS3ST3B1 IN HUMAN PROSTATE CANCER
... out in normal prostate epithelial cell line RWPE-1 Downregulating HS3ST3B1 has promoted cellular migration, invasion and proliferation as well as inhibited cellular adhesion via an upregulation ... prostate cancer cell lines and tissues The expression level of HS3ST3B1, a gene involved in heparan sulphate biosynthesis, was verified in prostate cancer cell lines LNCaP and PC-3 Silencing of this ... ducts and acini that are polygonal in contour Distinctive intraluminal ridges form the corrugations observed in the walls of the central zone (McNeal, 1998) Importantly, a layer of basal cells separates...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt
... protein homolog Ribosomal L1 domain containing Hepatocellular carcinoma-associated protein HCA10 Chromosome 20 ORF 30 Splicing factor proline ⁄ glutamine rich (polypyrimidine tract-binding protein ... Tca8113 ⁄ PYM cells and the extracellular pH was much lower in the same incubation conditions Generally, solid tumors maintain a high intracellular pH (pHi) but a low extracellular pH (pHe) Adaptation ... experimental well to that of a blank well, and the IC50 was also calculated Hoechst stain Cells in exponential growth were cultured with fresh medium in a six-well plate in which the coverslips had...
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báo cáo khoa học: " Expression of MICA, MICB and NKG2D in human leukemic myelomonocytic and cervical cancer cells" potx
... MICA and MICB, as well as NKG2D, we evaluated the possible expression of these proteins in two human epithelial cervical cancer cell lines, CALO and INBL, using polyclonal antibodies against ... strong cytoplasmic staining in both cell lines (Original magnification × 40) Figure Cervical cancer cell lines secrete MICA and MICB Cells (5 × 103) were cultured in 48-well plates for days, the ... phenylmethanesulfonyl fluoride, 1-μg/ml leupeptin, 1-μg/ml pepstatin, 1-μg/ml aprotinin and 0.1% Triton X-100) and resolved by SDSPAGE (10% acylamide) To obtain total cell lysates, 107 cells were...
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Báo cáo y học: " Candidate polyanion microbicides inhibit HIV-1 infection and dissemination pathways in human cervical explants" docx
... Goldberg CL, Wallenstein S, Profy AT, Klotman ME, Herold BC: PRO 2000 gel inhibits HIV and herpes simplex virus infection following vaginal application: a double-blind placebo-controlled trial J Infect ... MD) and Vero cells were grown in complete RPMI [RPMI 1640 medium supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 μg/ml streptomycin and mM L- glutamine]) The adherent cell line ... effect of polyanionic compounds against HSV-2 infection of epithelial cells ME180 cells (seeded at 1.5 × 104 cells/ well and cultured overnight), were exposed to HSV-2 (~5 × 104 Pfu/well) in the presence...
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Báo cáo khoa học: MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells potx
... is now well-established that miRNAs regulate a plethora of crucial cellular functions, including cell growth, differentiation and apoptosis, which are commonly altered in cancer cells In the present ... Nevertheless, the molecular signalling pathways modulated by miRNAs, which play a role in colorectal cancer, as well as cancer in general, are only partially characterized miRNA-143 and miRNA-145 ... epithelial crypts [27] and found mainly in the cytoplasm, where it was more intense in the ascending more differentiated pre-apoptotic glandular cells than in the immature, proliferating cells at...
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Báo cáo khoa học: Modulation of IMPDH2, survivin, topoisomerase I and vimentin increases sensitivity to methotrexate in HT29 human colon cancer cells docx
... washed in NaCl ⁄ Pi and resuspended in annexin-binding buffer Cells were collected, washed in NaCl ⁄ Pi and resuspended in binding buffer containing a final concentration of 0.5 lgÆmL)1 annexin V-FITC ... normalization using the APRT mRNA as the internal control Total extracts and Western blots Whole extracts were obtained from HT29 or HT29-R cells following the method of Kraus et al [58] Cells ... would involve a multitargeted antifolate Indeed, a novel inhibitor, pemetrexed, now in phase II trials, inhibits at least four enzymes involved in folate metabolism and purine and pyrimidine...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... expressed in all cell lines Following IL-1 treatment, COX-2 expression was markedly induced in WiDr cells, whereas the expression levels of cPLA2a, COX-1, cPGES and mPGES in each cell line were unaltered ... analyses revealed that, unlike mouse mast cells, human mast cells developed in the presence of SCF and IL-6 from cord blood cells [38] expressed only sPLA2-IID, but not the other sPLA2s including ... WiDr cells COX-1 was highly expressed in HT29 and WiDr cells and weakly expressed in KM2 0L2 cells COX-2 was detected only in WiDr cells The two terminal PGE2-biosynthetic enzymes, cPGES and mPGES,...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
... activity, and thereby stimulate cell spreading and motility in neuroblastoma cells [34] It is possible that different small G proteins are involved in LPA-induced cell motility in different cell types ... in turn recruits other proteins, including paxillin, p130Cas, vinculin and talin in focal adhesions These protein complexes anchor the actin cytoskeleton and provide structural integrity to cells ... focal adhesions of focal adhesion kinase and paxillin in endothelial cells J Biol Chem 272, 15442–15451 15 Ridley, A.J & Hall, A (1992) The small GTP-binding protein rho regulates the assembly...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
... stimulate proliferation of the seed cells In summary, the results demonstrate the effectiveness of TGF-β1 and IGF-1 in stimulating cell proliferation and maintaining morphology of human NP cells ... adding TGF(10 μg/ L) in the medium resulted in a total recovery of cell morphology to that of normal cells cultured in standard medium and a 96.5% increase of cell proliferation over the control ... in cell morphology and proliferation in samples cultured within medium containing TGF-β1 This is well supported also by previous studies in the literature Joon et al[9] cultured human IVD cells...
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Báo cáo lâm nghiệp:"Growth and biomass partitioning of Fagus sylvatica L. and Quercus robur L. seedlings in response to shading and small changes in the R/FR-ratio of radiation" pot
... of blue/ultraviolet-A light and light absorbed by phytochrome in controlling growth of pine (Pinus sylvestris L. ) seedlings, Planta 180 (1990) 212–216 [17] Gansert D., Sprick W., Storage and ... [20, 41] In addition silviculture in general 170 Ch Ammer will benefit from an improved understanding of the interactions between microclimate including light quality on tree ecophysiology as ... tree species) and variable Z2 (testing differences between shade treatments and control) Oak seedlings were taller under shade than in controls, while beech was taller in control In both cases...
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Báo cáo khoa học: "The conformation change of Bcl-2 is involved in arsenic trioxide-induced apoptosis and inhibition of proliferation in SGC7901 human gastric cancer cells" doc
... treatment Cells treated with different concentrations of arsenic trioxide (0 μmol /L, μmol /L, 10 μmol /L, 15 μmol /L, 20 μmol /L, 25 μmol /L and 30 μmol /L) in serum-free SGC7901 human gastric cancer cells ... SGC7901 cells; (b-f) 15 μmol /L arsenic trioxide-treated SGC7901 cells The cells were stained using DAPI staining B Untreated SGC7901 cells stained by anti-Bcl-2 N terminus antibody C SGC7901 cell stained ... Analysis SGC7901 cells were treated with different concentrations of arsenic trioxide (0 μmol /L, μmol /L, 10 μmol /L, 15 μmol /L, 20 μmol /L, 25 μmol /L and 30 μmol /L) for 24 hours The early and late...
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Báo cáo khoa học: "Real time PCR analyses of expression of E-cadherin, alpha-, beta- and gamma-catenin in human breast cancer for predicting clinical outcome" pps
... Following extensive washings, sections were incubated for 30 in the secondary biotinylated antibody (Multilink Swine anti-goat/ mouse/rabbit immunoglobulin; Dako Inc.) Following washings, Avidin ... sensitive and any contaminating cells (lymphocytes, stromal tissue) will lead to readings that belong to non cancer cells To overcome this problem, it is essential to isolate pure populations of ... cell-cell adhesion molecules E-cadherin, α-, β- and γ-catenin in human breast cancer by quantitative real time polymerase chain reaction The mRNA levels of these markers were related to clinicopathological...
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Báo cáo khoa học: "Neoangiogenesis in early cervical cancer: Correlation between color Doppler findings and risk factors. A prospective observational study" pot
... Transvaginal color Doppler ultrasound in the assessment of invasive cervical carcinoma Gynecol Oncol 1996, 60:26-29 Alcazar JL, Jurado M: Transvaginal color Doppler for predicting pathological response ... Figure cancer with color Doppler ultrasound Transvaginalabundant vascularization showing a cervical Transvaginal color Doppler ultrasound showing a cervical cancer with abundant vascularization ... Chen CL, Wang JF, Chang CF, Hsieh FJ: Clinical significance of intratumoral blood flow in cervical carcinoma assessed by color Doppler ultrasound Cancer 1995, 75:2518-2522 Tepper R, Zalel Y, Altaras...
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Báo cáo khoa học: "Costunolide causes mitotic arrest and enhances radiosensitivity in human hepatocellular carcinoma cells" potx
... coverslip coated with 50 mg/mL of Poly -L- Lysine Cells were incubated at 37 °C to allow attachment and spreading For immunofluorescence staining, the cells were fixed with 3% formaldehyde for 10 minutes ... of duplicated chromatid pair aligned in the center of dividing cells At least 200 cells per field in a minimum of five randomly selected fields were counted on three slides for each sample Detection ... dried and cells were fixed with 4% paraformaldehyde in PBS (pH 7.4) and mounted in Vectashield mounting medium with 1.5 Ag/mL 4V, 6-diamidino-2phenylindole (Vector Laboratories, Inc., Burlingame,...
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