and or activity of intestinal bacter

... Construction of HspH and HspF derivatives for structure-function studies In order to study the principles of a-Hsp assembly and the relation between complex formation and chaperone activity, we ... Biology of Heat Shock Proteins and Molecular Chaperones (Morimoto, R.I., ` Tissieres, A & Georgopoulos, C., eds), pp 335–373, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York Ehrnsperger, ... sufficient for dimer formation This argues that more residues towards the N-terminus are involved in dimerization Importance of the N-terminal region Additional portions of the N- and C-terminal...

Ngày tải lên: 31/03/2014, 21:21

... plasmid pQI-TorD [19], which incorporates a C-terminal hexahistidine tag onto the protein For constitutive expression of torD, plasmids pSU-TorD [5] and pUP-TorD [5], derivatives of pSUPROM and pUNIPROM ... these factors are often referred to as ‘clocks’, ‘switches ⁄ adaptors’ and ‘sensors’, respectively [31] The turnover number of TorD for GTP of 3.9 min)1 is within the range expected for classical ... coli TorD homodimer has GTPase activity D Guymer et al tion coefficients of 30 940 MÆcm)1 66 350 MÆcm)1 for FMalE-TorAhis Experimental procedures for TorDhis and Plasmids Overproduction of TorDhis...

Ngày tải lên: 16/02/2014, 09:20

... key residues that are important for CD2–CD58 interaction This correlates with the higher inhibitory activity of the cEL peptide and the very low inhibitory activity of the cYT peptide compared ... conformation of backbone had an interproton error of less ˚ than 0.2 A compared to upper and lower boundaries of distances from ROE/NOE data and (b) the conformation had / angles within 30° of ... is also an important residue in the interface of hCD2–hCD58 Comparison of hCD2 and rat CD2 3D structures indicated that Tyr86 and Glu36 side chains overlap in hCD2 and rat CD2 and oriented in the...

Ngày tải lên: 19/02/2014, 13:20

... specific 5¢ -and 3¢ primers selected on the basis of the published sequences for tomato b-fructofuranosidase (GenBank accession no D11350 and S70040) Primers for the short isoform of b-fructofuranosidase ... truncated isoform Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant expression in E coli For protein expression in E coli, only the cDNA coding for the mature ... reactivity to nLyc e and CCD (C,D) Patient 2, showing no IgE reactivity to nLyc e and CCD Standard deviations are shown for each measurement horseradish peroxidase: horseradish peroxidase, dhorseradish...

Ngày tải lên: 21/02/2014, 00:20

... to the column and eluted with a linear gradient of solvent A (chloroform/ methanol : 4, v/v) and solvent B (chloroform/methanol/ water : : 2.5, v/v/v) starting with 0% solvent B for 30 min, then ... suspension by the method of Bligh and Dyer [18], and the organic layer was concentrated to dryness on a rotary evaporator Lipids (0.2 gÆg)1 dried cells) were redissolved in chloroform/methanol : (v/v) ... CHO reporter system In order to investigate the potential involvement of TLR2 and TLR4 in the recognition and signal transduction of the glycolipids, we analyzed the stimulatory activity of MfGl-II...

Ngày tải lên: 21/02/2014, 00:20

... cofactor is likely to affect the kinetics of catalysis of either the reductive or oxidative half-reaction, or both Such an effect could be either due to altered redox power of the cofactor and ... for atomic coordinates and structure factors are deposited with the Research Collaboratory for Structural Bioinformatics Protein Data Bank 784 FEBS Journal 276 (2009) 776792 ê 2008 The Authors ... plasmids of variants L537G and L537W as templates for the PCR reactions, the forward primers P2OE542K-for and P2OE542R-for, and the reverse primers Double-G-rev (5Â-CATGAATT GCGGGCCGGAGCCG-3Â) and...

Ngày tải lên: 07/03/2014, 03:20

... discussions The authors acknowledge the beamline staff at ID14-EH3 for technical assistance, and the ESRF and the Norwegian Research Council (project Sygor) for financial support This work was funded ... Heidelberg, for provi¨ ding us with vector pETM11, Gustav Vaaje-Kolstad for help with the chitin degradation experiments and helpful discussions, and May Bente Brurberg and Svein Horn for helpful ... TOPO vectors for insertion in an expression vector, using BsmBI and KpnI for cloning into NcoI–KpnI-digested pETM11 vector (Gunter Stier, ¨ EMBL, Heidelberg, Germany) The pETM11 vector contains...

Ngày tải lên: 07/03/2014, 11:20

... tool for exploring the viral determinants important in virus–cell interactions, and to find permissive cell lines for further molecular and cell biological studies of this peculiar human virus For ... when cloned as tandem repeats [29] and CAV requires either tandem-repeat cloning or excision of the viral genome from the plasmid before transfection [30] The rescue and replication of the viral ... USA) and 17.5 U of enzyme mix (‘Expand High Fidelity PCR System’; Roche) The PCR comprised annealing at 55 °C for 30 s and extension at 72 °C for for the first ten cycles, with addition of sÆcycle)1...

Ngày tải lên: 16/03/2014, 05:20

... protein kinase (DMPK) isoforms and truncation mutants Structural domain organization of DMPK splice isoforms A, C, E and F, and N-terminal and C-terminal truncation mutants of DMPK E used in this ... for homo- and heteromultimerization of DMPK isoforms and that multimerization has a function in substrate binding, phosphorylation and subcellular targeting properties of individual DMPK isoforms ... use of Pfu polymerase and specific primers EcoRI and XhoI sites were incorporated in the forward and reverse primers, respectively (underlined, see below) DNA fragments were cut with EcoRI and...

Ngày tải lên: 16/03/2014, 13:20

... Fig Autoradiogram of BphP Autoradiogram after [32P]ATP[cP] labeling, SDS ⁄ PAGE and electroblotting (A) Autoradiogram of the apo and holo forms of BphP (B) Stability of the autophosphorylation ... Japan) and Lagarias (UC Davis, Davis, CA, USA) for the gift of chromophores We thank Maria Sowa and Thorben Dammeyer for technical assistance This work was supported by the EmmyNoether-Program of ... after incubation of purified apo-BphP and holo-BphP (Pr and Pfr form) with [32P]ATP[cP] Both forms of BphP displayed autophosphorylation activity (Fig 6A) Although the Pfrenriched form shows slightly...

Ngày tải lên: 16/03/2014, 18:20

... nature of the end groups and some other residues of citropin 1.1 is not as important as it is for antibiotic or anticancer activity For example, compare the data for changes in Gly1, Phe3, Ser11 and ... Gram-negative organisms for Gly14 and Gly15 The conclusions from this study are that (a) modification of either of the terminal residues reduces the activity, and (b) the activity against Gram-positive organisms ... anticancer and antibacterial activity of membrane active peptides, suggests that the anticancer activity is also due to penetration and disruption of the membranes of the cancer cells The selectivity of...

Ngày tải lên: 17/03/2014, 09:20

... from ventricles of 1–2 day-old Sprague– Dawley rats and cultured as described earlier [16] Experiments conformed with the Guide for the Care and Use of Laboratory Animals (NIH) and were approved ... P/ACE-SYSTEM MDQ software (Beckman Coulter, Fullerton, CA, USA), and were normalized to the area of the internal standard e-fluoresceinyl lysine in order to eliminate irregularities of injection and buffer ... uptake for both I and II No differentiation between the permeation behavior of I and II appears possible on the basis of the CLSM data, except that a lower rate of re-export became apparent for the...

Ngày tải lên: 23/03/2014, 13:20

... secondary structure of these two ligands of ODN receptors should provide useful information for the design of potent, stable and selective agonists and antagonists of these receptors As ODN exerts ... chemistry for increasing the receptor affinity and selectivity of peptide ligands [13,14,27] The Ala-scan of OP has revealed that the side chain of each residue is required for the full activity of the ... spectrum, supported the existence of a well-defined structure and were used to drive a set of 27 distance and four dihedral angle constraints for the trans conformer and 31 distance and six dihedral...

Ngày tải lên: 24/03/2014, 04:21

... abrogated tetramer formation It appears that the higher mobility of the glycine-rich loop is incompatible with binding of NADPH and ⁄ or delivery of a hydride to the flavin cofacor and hence K109 plays ... coefficient of enzyme-bound FMN, 0.2% SDS was used to release the cofactor UV ⁄ visible absorbance spectra were recorded before and after denaturation of the enzyme with a photometer (model specord 205) ... residue 109 Orange to red colours and a wider tube indicate regions with higher B-factors, whereas shades of blue and a narrower tube indicate regions with lower B-factors The FMN cofactor is shown...

Ngày tải lên: 30/03/2014, 01:20

... patterns of structure or charge found in human SP-B or SP-C, appear to mimic some of the structural and functional properties of the native proteins and thus may offer a useful basis for the design of ... pathogens and toxins, and modulate the function of respiratory inflammatory cells [1–4] The alteration or deficiency of this system leads to respiratory distress The main phospholipid constituent of pulmonary ... al [16] and Gustafsson et al [17], who studied the secondary structure of relatively high concentrations of KL4 incorporated in monolayers or bilayers in the absence of Ca2+ Cai and co-workers...

Ngày tải lên: 30/03/2014, 11:20

... activity of the LlA and EcAIII proteins With some of the substrates, no enzymatic activity could be detected, indicating lack or possibly very low level of the corresponding activity Enzyme activity ... a Laboratory Manual, Cold Spring Harbor Laboratory Press, New York 56 Liu, Y., Guan, C & Aronson, N.N (1998) Site-directed mutagenesis of essential resiudes involved in the mechanism of bacterial ... NaCl and 0.5% (w/v) glucose for large-volume cultures When required, either ampicillin or chloramphenicol was added at a concentration of 100 or 25 lgÆmL)1, respectively Cloning of LlA and EcAIII...

Ngày tải lên: 30/03/2014, 15:20

... nature of the interactions required for the stimulatory and inhibitory effects of protein B Either the two types of effect result from binding at different sites on the hydroxylase, or the conformational ... degradation offers a mechanism by which their activity, and hence the activity of the whole enzyme complex, could be controlled It also offers an explanation for the persistence of the regulatory components ... analysis revealed the presence of two major molecular ions, of 16 300 Da (corresponding to the mass of the intact G13Q mutant) and 14 700 Da (corresponding to the mass of a truncate beginning at...

Ngày tải lên: 31/03/2014, 09:20

... shift of more than 10 nm indicates that the tryptophan residue of D16W-D24)37 GGN4 anchors into the hydrophobic core of the SDS micelle, as shown in the case of tryptamine, more efficiently or more ... 2002 Structure activity relationships of GGN4 analogues (Eur J Biochem 269) 4373 REFERENCES Fig Fluorescence quenching and blue shift Fluorescence emission spectra of NATA (a and b) and D16W-D24)37 ... excitation and emission bandwidth, a 0.5-s response time, and a scan speed of 40 nmÆs)1 All samples contained lM peptide or the same concentration of NATA for control experiments, in water or a 10-mM...

Ngày tải lên: 31/03/2014, 09:20

... interests The authors CL, ND, PB, LG, RS, and AD declare that they are employees of Centocor, Inc which provided supported for this work Authors' contributions CL generated reagents and performed the ... to better understand the mechanism of action of HRSV neutralizing antibodies and the impact of resistance to these antibodies upon the fusion activity of the F protein A summary of these results ... a critical residue for the binding of ch101F Mutation of K433 to D, L, N, Q, R, or T abolished ch101F binding as previously reported [20], but, with the exception of K433D and K433R, modest to...

Ngày tải lên: 18/06/2014, 18:20

... cross-correlation analyses using Equation (1) to compare averaged electromyographic waveforms and kinematic profiles of control subjects with the profiles of each spinal cord injury subject with and ... with spinal cord injury walking with (MA) and without (WO) manual assistance and control (C) subEMG profiles for EMG profiles for subjects with spinal cord injury walking with (MA) and without ... with spinal cord injury walking with (MA) and without (WO) manual assistance and control (C) subEMG profiles for EMG profiles for subjects with spinal cord injury walking with (MA) and without...

Ngày tải lên: 19/06/2014, 10:20