and its effect on penaeus monodon oocyte development in vitro

... Environment Fish and Marine Service Biology Station, St Andrews 14 Darbyson E, Swain DP, Chabot D, Castonguay M (2003) Diel variation in feeding rate and prey composition of herring and mackerel in ... (1991) Carbonate deposits in marine fish intestines: a new source of biomineralization Limnol Oceanogr 36:1227–1232 12 Hickman CP Jr (1968) Ingestion, intestinal absorption, and elimination of seawater ... and Thailand [3], and in low-salinity inland saline water ranging from 0.5 [4] to 28.3 ppt [5] Around 30% of commercial shrimp farming in Thailand is conducted using low-salinity water [3], and...

Ngày tải lên: 19/07/2016, 19:43

... Osmotic and chloride regulation in the hemolymph of the tiger prawn Penaeus monodon during molting in various salinities Marine Biology 95:377-385 Funge-Smith S.J., A.C Taylor, J Whitley and J.H ... J.A Martinez-Paz and L Chim 2002 Combined effect of temperature and salinity on osmoregulation of juvenile and subadult Penaeus stylirostris Aquaculture 209:307-317 Lignon J.M 1987a Ionic permeability ... comparison of osmotic, sodium and chloride concentrations between the urine and hemolymph of Penaeus seti ferus (L.) and Penaeus stylirostris stimpson Comp Biochem Physiol 70A:525-528 Chen J.C and...

Ngày tải lên: 02/04/2014, 10:20

... Osmotic and chloride regulation in the hemolymph of the tiger prawn Penaeus monodon during molting in various salinities Marine Biology 95:377-385 Funge-Smith S.J., A.C Taylor, J Whitley and J.H ... J.A Martinez-Paz and L Chim 2002 Combined effect of temperature and salinity on osmoregulation of juvenile and subadult Penaeus stylirostris Aquaculture 209:307-317 Lignon J.M 1987a Ionic permeability ... comparison of osmotic, sodium and chloride concentrations between the urine and hemolymph of Penaeus seti ferus (L.) and Penaeus stylirostris stimpson Comp Biochem Physiol 70A:525-528 Chen J.C and...

Ngày tải lên: 22/07/2014, 11:21

... depends on both the concentration of free PMLA and the inhibition constant This parameter reflected the affinity of the polymerase and both the affinity and concentration of competing ligands for binding ... terminated by fixation in a 10-mL solution containing ice-cold 5% saturated trichoroacetic acid and 50% acetone The samples were disrupted in a Dounce homogenizer and filtered on GF/C After washing with ... polyanion In the (added) nuclear extract, such ligands were histones, and probably other DNAbinding proteins [2] To obtain an optimal response by the reporter polymerase to a varying inhibitory...

Ngày tải lên: 31/03/2014, 21:21

... in Mainland China and Taiwan Province of China and subsequently spread to the Philippines, Indonesia, Viet Nam, Thailand, Malaysia and India (RAP, 2004) In 2008, 67% of the world production of ... widely cultured in countries of Asia-Pacific The introduction of white shrimp to Asia has given rise to a bloom in farming of this species in China, Thailand, Indonesia and Viet Nam in the last decade, ... information on the mineral needs of shrimps Most of the information available for mineral requirements has been done under laboratory conditions using purified or semi-purified diets and scanty information...

Ngày tải lên: 22/09/2015, 12:38

... uorescence intensity in the presence of protein compared to the uorescence intensity in its absence [23,42] Determination of eosin binding to the fusion proteins Interaction of eosin Y with GST fusion ... binding to the (L354I777)GST fusion protein that contains both the N- and P-domains Titration in fact revealed that TNP-ATP binds to a single site only, as tting ể FEBS 2004 Mapping of ATP and ... Na+/K+ ATPase [55] In conclusion, neither method revealed any indication of a conformational change of the N-domain upon ligand binding in the investigated GST fusion protein It is quite evident...

Ngày tải lên: 19/02/2014, 16:20

... aggregation of lens proteins and enzymes These protective capabilities have been demonstrated with other, in vitro systems, including prevention of aggregation of insulin B chain following reduction ... system to maintain membrane integrity [19] The lysed cell suspension was divided equally into two tubes and to one tube a-crystallin (1 mgÆmL)1) was added and incorporated on resealing For controls, ... for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose (50 mM) to determine...

Ngày tải lên: 17/03/2014, 03:20

... tight association of the diprotomer [28], pressure effects on these two reactions hold promise for use in methodology in discriminating conformational changes Effects of pressure on intrinsic ¯uorescence ... digestion also suggests that there are few tritium atoms in the contact surface of a and b subunits All things considered, tritium incorporation mainly occurs in the interface of protein and lipid ... hydrocarbon region [43,45,46] These changes would be expected to alter the environment of transmembrane proteins in the contact surfaces of proteins and water, and/ or protein and lipid bilayer: an increase...

Ngày tải lên: 24/03/2014, 00:21

... accumulation of cAMP Monensin Increases Na+ concentration Insulin/IGF-1 Increases Na+ affinity of NKA Possible translocation role Veratridine Increases Na+ influx per action potential Excitation Increases ... Corticosteroids have long-term effects on NKA regulation inducing both mRNA and protein expression (Therien & Blostein, 2000) Dexamethasone infusion in rats for 7-14 days increased NKA content in SOL, EDL, ... Calcium ion K+ Potassium ion [K+] Potassium ion concentration [K+]a Arterial potassium ion concentration [K+]I Interstitial potassium ion concentration Na+ Sodium ion [Na+] Sodium ion concentration...

Ngày tải lên: 04/12/2015, 13:59

... activity due to increasing the KCl concentration On the other hand, increasing the Na+ concentration had no effect on the ouabain-insensitivity of pNPPase activity The concentration giving halfmaximal ... activity and ion-binding pNPPase activity and ion binding were measured in reaction mixtures containing 0.1 mm RbCl, 0.5 mm pNPP and 0–10 mm NaCl (Fig 4) In this experiment, the MgCl2 concentration ... radioactivities of reaction mixtures with native and acid-denatured enzymes Determination of protein and oligomycin concentration Protein and oligomycin concentrations were determined as described elsewhere...

Ngày tải lên: 07/03/2014, 16:20

... separated into Triton-soluble (S) and Triton-insoluble fractions The insoluble fraction was further separated into DNaseI-soluble (S3) and DNaseI-insoluble (P¢) fractions Proteins in these fractions ... MCM proteins on licensed chromatin in the G1 phase was detached from chromatin Phosphorylation of MCM4 and other factors may be involved in stronger binding of MCM4 protein to chromatin in the ... dehydration, the sections were embedded in Epoxy resin (Epon 812, TAAB, Aldermaston, England) Thin sections were obtained by cutting on a LeicaUltracut UCT ultramicrotome and collected on grids Sections...

Ngày tải lên: 16/03/2014, 13:20

... trimethylation marks were localized in the less intense DAPI-stained regions in the nucleus (representing less condensed euchromatin), leaving gaps in the more intense DAPI-stained regions (representing ... acetylation and anti-H3S28 phosphorylation sera Cyclin B and E expression and H3S28 phosphorylation were used as markers for cell synchronization Coomassie stain for histone was used as loading control ... shown) These results indicate that MLL1 and its interacting proteins dissociate from mitotic chromatins, spread into the cytoplasm and coordinate in a similar fashion during the cell cycle FEBS...

Ngày tải lên: 23/03/2014, 06:20

... elicited by the interaction of a minor fraction of ouabainaltered Na+/K+-ATPase with neighbouring proteins (Src, growth factor receptors and Ras), i.e by protein–protein interaction In myocytes, ... signal-transduction were interpreted as independent of a small increase in [Na+]i and also occasionally independent of the subsequent increase in [Ca2+]i [10], whereas in other publications, activation of ... ouabain but not the significant content of ouabain-sensitive a2 in rat cardiac muscle [23–25] The ratio between the a1-containing insensitive and a2 /a3-containing sensitive Na+/K+-ATPase in rat...

Ngày tải lên: 23/03/2014, 17:21

... distinct from ouabain’s effect on [Na+]i and [K+]i is also indicated by the intriguing recent demonstration of ouabain-induced slow calcium oscillations and associated NF-jB activation in renal epithelial ... within the plasma membrane with two distinct functions: one being the classical pool of the enzyme as an energy transducing ion pump whose partial inhibition by ouabain initiates the increase in ... clearly indicated that the same nontoxic concentrations of ouabain that cause partial inhibition of Na+/K+-ATPase and an increase in cardiac contractility, also stimulate myocyte growth and protein...

Ngày tải lên: 24/03/2014, 00:21

... the connecting line and looking at only the data for the varied exponential line shows that one cannot eliminate exponential growth by a straight line on rectangular coordinates (d) Comparison ... of exponential and linear growth Comparison of experimental measurements of exponential and linear growth (a) Plotting of exponential and linear growth over one doubling shows that the lines are ... Thus, in the first interval 0.1 units would be added, in the second 0.1 units again, and so on The difference between exponential and linear growth is that with exponential growth the absolute increase...

Ngày tải lên: 13/08/2014, 23:20

... that during infection, the contribution of the minor proteins to cell destruction via apoptosis is only moderate, suggesting that toxicity of the minor proteins is controlled during the infectious ... followed by incubation of cells with rabbit anti-GFP IgG diluted in NaCl ⁄ Pi containing 0.5% BSA and 0.1% fish gelatine (pH 7.4) After washing in NaCl ⁄ Pi containing 0.1% BSA, sections were incubated ... contains one molecule of either VP2 or VP3 [1] VP1 protein is responsible for the interaction of MPyV virions with the ganglioside GD1a and GT1b receptors [2] Its N-terminus contains basic amino...

Ngày tải lên: 16/02/2014, 09:20

... generation to glial cell generation, and to be related to the expression of granuphilin ⁄ slp4 in insulin-producing cells [28] Moreover, miR9 and other neuronal miRs including miR125b and miR128, ... differentiation, and neuronal diseases In addition, the in vitro and in vivo imaging systems will undoubtedly provide information about the molecular patterns and mechanisms of miR biogenesis in various ... are then incorporated into the RNAinduced silencing complex and bound to the 3¢-UTR of its target mRNA to induce either mRNA degradation or translational inhibition [2,14,15] Interestingly, unlike...

Ngày tải lên: 18/02/2014, 17:20

... enhancing the conformational change in path C, and enabling a conformational change in path A, thereby increasing the rate of both cycles In reaction path A, binding of Mg2+ to subunit induces a conformational ... depending on the Zn2+ concentration, thus, indicating that Zn2+ does not in uence the equilibrium concentration of the subunits Deviation from linearity in the dependence of the Mg2+ ion concentration ... [19] Curves and kinetic constants, describing competitive inhibition, were obtained from respective data by applying the corresponding equation for competitive inhibition, using the kinetic parameters...

Ngày tải lên: 21/02/2014, 00:20

... represented in continuous line, second heating in dashed lines, third heating in dotted lines and fourth heating in dashed-dotted lines equilibrium at low concentration are the native, N, and the ... deconvolution methods The results presented here on the thermal denaturation of SK and its domains help to clarify inconsistencies existing in previous reports concerning the number of cooperative folding ... domain B Nevertheless, interdomain contacts may be conditioned by the complex formation with microplasmin in the crystal structure An alternative explanation could be that some interactions internal...

Ngày tải lên: 21/02/2014, 03:20

... transcription factors [89], proteins involved with DNA replication [90], ribosomal proteins [91], and the like In contrast, eukaryotic proteins involved in basic metabolic functions, in particular ... mitochondria and is attached to the inner membrane, where it is a component of complex II, which contains a cytochrome b, an anchor protein, and several additional subunits in the inner mitochondrial ... compartmentalized in chloroplasts and mitochondria and thought to have once been encoded in the respective organellar DNA, one can gain insights into the evolutionary dynamics of (a) pathway evolution, (b)...

Ngày tải lên: 22/02/2014, 04:20