and intersubstrate interactions during in vitro de and remineralisation studies
Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
Ngày tải lên :
20/02/2014, 01:20
... synthesized in one round of synthesis, we performed RdRp assays in the presence of heparin in order to prevent reinitiation In a first step we determined the heparin concentration needed to prevent reinitiation ... the minusstrand RNA being at that time unavailable In the present study we investigated the involvement of sequences and ⁄ or structures in RNA synthesis directed in vitro by HCV NS5B by using ... HCV minus-strand RNA is illustrated in Fig This fragment contains two domains: domains FEBS Journal 272 (2005) 3872–3886 ª 2005 FEBS Binding and replication of 3¢-end of HCV minus RNA I and II...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
Ngày tải lên :
20/06/2014, 07:20
... steroidogenic acute regulatory protein in the rodent ovary and placenta: alternative modes of cyclic adenosine 3', 5'-monohposphate dependent and independent regulation Endocrinology 2009, 150:977-989 ... Follicle-Stimulating hormone (FSH) stimulates phosphorylation and activation of protein kunase B (PKB/Akt) and serum and glucocorticoid-induced kinase (Sgk): evidence for A kinase-independent signaling by ... reported that during the ovulation process the PKA-dependent pathway involving the ligand activation of G protein-coupled receptors by their ligands in cumulus and/ or granulosa cells induced the...
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báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt
Ngày tải lên :
12/08/2014, 03:20
... gene in lines 156, 49 and 111 In addition, T-DNA tandem repeats were identified in lines 156 and 49, and vector backbone sequences were integrated in lines 17, 156, 49 and 179 (data not shown) Despite ... low incidence of single copy T-DNA insertions in banana tagged lines, we demonstrate here that activated insertion(s) can be identified in multicopy T-DNA mutants The combination of I-PCR and ... also identified in promoter sequence 17-1 and could contribute to increased expression observed during the LT treatment In summary, we have devised and applied a real-time in vitro screening platform...
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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx
Ngày tải lên :
22/02/2014, 07:20
... an increase in DNA laddering during incubation: inhibition was 45% compared with nontreated incubation at h With 30 mM NAC, inhibition of DNA fragmentation was started at h incubation, and inhibition ... of Mn- and Cu/Zn-SOD proteins during in vitro incubation of chorion laeve tissue Western blotting analyses (A) and (B) show Mn- and Cu/Zn-SOD protein expressions, respectively Values indicated ... glucocorticoid Next, we investigated the existence and quantitative alteration of Mn-SOD and Cu/Zn-SOD proteins in chorion laeve tissue during in vitro incubation using Western blotting analysis With...
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ỨNG DỤNG KỸ THUẬT TIÊU HÓA IN VITRO ĐỂ ĐÁNH GIÁ SỰ TIÊU HÓA DƯỠNG CHẤT VÀ SẢN XUẤT RƠM DINH DƯỠNG (NUTRITIONAL RICE STRAW) LÀM THỨC ĂN CHO TRÂU BÒ pptx
Ngày tải lên :
11/03/2014, 06:21
... hóa vật chất hữu in vitro với dịch cỏ tương tự kết tiêu hóa vật chất hữu in vitro phân (Bảng 6) Nhưng tỉ lệ tiêu hóa vật chất in vitro dịch cỏ cao tỉ lệ tiêu hóa vật chất hữu in vitro phân điều ... Cần Thơ - Phương pháp in vitro với nguồn vi sinh vật từ phân phương pháp in vitro với nguồn vi sinh vật từ dịch cỏ có mối quan hệ chặt chẽ với Tỉ lệ tiêu hóa vật chất hữu in vitro dịch cỏ cao tỉ ... htm MINITAB, 2000 Minitab reference manual release 13.20 Minitab Inc Thu, N V 2005 A study of enriched-nutrient rice straw (ES) on in- vitro digestibility, and feed utilization, milk yied and composition...
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Báo cáo khoa học: Sử dụng kỹ thuật nuôi cấy in vitro để nghiên cứu sự phát triển của phát hoa dendrobium sonia potx
Ngày tải lên :
27/06/2014, 13:20
... cellulaires d’espèces diploides et triploides Thèse Doctorat Université Paris XI (Orsay), 84p., (1992) [3] King R.W and Evans L.T Gibberellins and flowering of grasses and cereals: prizing open the lid ... 1mg/l zeatin, 0,5mg/l AIA and 1mg/l GA3 With 5mg/l BA, inflorescence meristem formed vegetative buds instead of developing of inflorescence Roles of plant growth regulators on longevity of inflorescence ... N The induction and maintenance of flowering in Impatiens Development, 124:3343-3351., (1997) [5] Sobry S., Havelange A., Liners F., Cutsem P.V Immunolocalization of homogalacturonans in the...
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Báo cáo khoa học: "Induction in vitro de l’enracinement de microboutures d’Acacia tortilis subsp. raddiana par traitement transitoire à l’auxine" ppsx
Ngày tải lên :
08/08/2014, 14:21
... meilleur traitement inducteur de l’enracinement de microboutures d’A tortilis subsp raddiana in vitro combine l’ANA comme auxine (10 mg L–1) et une faible dose de cytokinine (kinétine 0,01 mg L–1) ... 28 jours de culture sur milieu d’expression Les traitements étudiés (variables indépendantes) sont d’une part les deux durées de l’induction de l’enracinement Enracinement in vitro de microboutures ... plus de racines par vitroplant pour 69 % 73 % des vitroplants enracinés Parmi les vitroplants traités l’ANA, la meilleure combinaison associe l’ANA 10 mg L–1 la Kinétine 0,01 mg L–1 (57 % de plants...
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Báo cáo khoa học: "microgreffe, une solution pour la multiplication in vitro de l’Acacia senegal (L) Willd ?" pps
Ngày tải lên :
08/08/2014, 18:21
... développons une méthode de clonage des A senegal pour l’amélioration de la production de gomme arabique, par greffage in vitro en fente terminale, d’apex ou de microboutures issus de rameaux rajcunis ... l’étude de certains aspects de la multiplication de l’Acacia senegal (L) Willd Thèse de doctorat en sciences, université de droit d’économie et des sciences d’Aix-Marseille, France (1983) Étude de ... Greffage in vitro d’apex sur des plantules décapitées de Pommier (Malus pumila Mill).CR Acad SciParis 287, 249-253 Clarke AE, Anderson RL Stone BA (1979) Form and function of arabinogalactans and...
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Báo cáo lâm nghiệp: "Internal levels of plant growth regulators during in vitro culture of wild cherry (Prunus avium L.)" doc
Ngày tải lên :
09/08/2014, 04:20
... Epstein and Lavee (1986) reported a transformation of IBA into IAA during in vitro culture of Vitis vinifera and Olea europea The chemical pathway could be a 0-oxidation of the butyric side chain, ... horticoles et culture in vitro Rev For Fr 38, 60-68 Epstein E & Lav6e S (1986) Conversion of indole-3-butyric acid to indole-3-acetic acid by cuttings of gravepine (Vitis vinifera) and olive (Olea ... culture in vitro Plant Cell Tissue Organ Culture 1, 155158 Label P., Maldiney R., Sossountzov L., Cornu D & Miginiac E (1989) Endogenous levels of abscisic acid, indole-3-acetic acid and benzyladenine...
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Báo cáo lâm nghiêp: "Multiplication végétative in vitro de quelques espèces d’ormes" doc
Ngày tải lên :
09/08/2014, 06:22
... Contamination is a limiting factor in in vitro development The best results were obtained either by sampling herbaceous shoots growing in the open air or after forcing under glass, or using the ... capacity during in vitro reproduction Maintenance of plants in vitro Results were obtained for the clones maintained at °C with hours of light (7 W/m After ) r rooting, the plants were maintained ... l’aptitude au bouturage au fur et des cycles de multiplication in vitro Ainsi pour OCBa, sans régulateur de croissance, le p 100 d’enracinement 50 jours passe de 50 100 entre et ans de culture in vitro...
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Báo cáo y học: "Characterization of vascular strain during in-vitro angioplasty with high-resolution ultrasound speckle tracking" ppt
Ngày tải lên :
13/08/2014, 16:20
... 4-cm length) was inserted into each artery The standard, noncompliant balloon was expanded manually using linearly increasing pressure while observing the pressure sensor reading during ultrasound ... for observing strain patterns during angioplasty balloon deformation in the ultrasound B-scan image and determined the regions on the vessel wall where longitudinal strain, shear strain and average ... imaging’s ability to detect histologically definable characteristics within the vessel These findings distinguished vascular collagen fiber wall unfolding from fiber deformation or strain during...
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Blastocystis investigations on host pathogen interactions using in vitro model systems
Ngày tải lên :
11/09/2015, 09:11
... animals Accumulating evidence over the last decade suggests association of Blastocystis with gastrointestinal disorders involving diarrhea, abdominal pain, flatulence and vomiting Despite new knowledge ... bowel disorder characterized by abdominal pain and changes in bowel habits Interestingly, some studies have also reported symptoms like itching (Garavelli and Scaglione 1990) and joint pain (Lee ... immune responses in order to improve survival in the gut Other isolated clinical reports described intestinal inflammation and edema in patients infected with Blastocystis (Kain et al 1987; Russo...
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NUÔI CẤY VÀ TẠO RỄ CÂY HOÀN NGỌC (Pseuderanthemum Bracteatum) TRONG ĐIỀU KIỆN IN-VITRO ĐỂ THU NHẬN BETULIN- HỢP CHẤT THỨ CẤP
Ngày tải lên :
11/01/2016, 13:52
... Thân in vitro Giai đoạn Phương pháp thu Cây hái mẫu Cây hái mẫu Cây hái mẫu Cây in vitro 12 tuần Cây in vitro HPLC-UV 12 tuần Cây in vitro 12 tuần Cây in vitro 12 tuần tuần Cây in vitro tái sinh ... in vivo Cây hái mẫu in vivo Cây hái mẫu in vivo Cây in vitro 12 tuần Cây in vitro 12 tuần Cây in vitro 12 tuần Cây in vitro 12 tuần Phương pháp Hàm lượng Nhận xét (µg/g) 0,85 Hàm lượng betulin ... từ in vitro hoàn ngọc 44 Hình 14 : Rễ biệt hóa từ mô sẹo thân non in vitro 47 Hình 15: Cây in vitro tái sinh từ mô sẹo sau tuần nuôi cấy 49 vi Hình 16: Cây in vitro tái sinh...
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Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
Ngày tải lên :
07/03/2014, 02:20
... between gastrins and iron homeostasis was first provided in a search for gastrinbinding proteins in porcine gastric mucosa [14] An interaction between Gamide and transferrin was identified by covalent ... polypeptide chain (consisting of 680–690 amino acids) has been determined in both diferric [3] and iron-free [apo-transferrin (ApoTf)] forms [4] The chain is folded into two lobes, the N-lobe and ... interaction with transferrin, is obviously essential to a full understanding of the interaction The independent involvement of iron [17] and nonamidated gastrins such as Ggly [10] in the development of...
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báo cáo hóa học: " Gliovascular and cytokine interactions modulate brain endothelial barrier in vitro" docx
Ngày tải lên :
19/06/2014, 22:20
... timing, and the cytokine involved Several studies have tried to determine mechanisms through which astrocytes modulate endothelial barrier using contact-dependent and independent co-culture models ... surrounding the insert (in contact-dependent or contact-independent systems) We observed an increase in mouse brain endothelial barrier with IFN-g over the other cytokines or controls at all time points ... (HCMEC-D3) and human astrocyte contact dependent co-culture barrier Control Under control conditions contact dependent HCMECD3/HFA co-cultures’ (incubated in 10% EBM2 in the apical side and 10% DMEM in...
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Báo cáo lâm nghiệp: "Polyamines and ethylene during of Prunus avium L. in vitro rooting" pps
Ngày tải lên :
09/08/2014, 04:20
... outlined above suggest that polyamines may be involved in the rooting process, probably in the stages of active cell division In fact, increases in intracellular PUT and SPD levels preceded root ... medium) incorporation into ethylene Ten shoots were placed in small flasks equipped with a side-arm, into which filter paper soaked in KOH was I inserted, and a center well containing either 0.1 M ... 12-15 d and was thus used in all subsequent experiments Endogenous polyamine content during the rooting phase was characterized by a peak in PUT levels on d 9-11 in all shoot portions Spermidine...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... Reaching a genetic and molecular understanding of skeletal development Dev Cell 2002, 2:389-406 48 Hartmann C, Tabin CJ: Dual roles of Wnt signaling during chondrogenesis in the chicken limb Development ... with 100 μL of binding buffer, fixed overnight in PBS-buffered 4% paraformaldehyde, dehydrated, infiltrated with isoamylacetate (Merck, Hohenbrunn, Germany), embedded in paraffin, and sectioned ... minutes, resuspended in complete DMEM (containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin), and plated at to × 107 nucleated cells per 75 cm2 flask (Falcon, Beckton Dickinson Labware,...
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Báo cáo y học: "Analysis of host- and strain-dependent cell death responses during infectious salmon anemia virus infection in vitro" doc
Ngày tải lên :
12/08/2014, 04:21
... PBS and trypsin-EDTA (Invitrogen, Carlsbad, CA, USA) was added The trypsinized cells and media containing detached cells were pooled and pelleted by centrifugation The staining with Annexin V-FITC ... characterized by cell shrinking, rounding and detachment from the substratum, was evident from day The detachment of cells increased during the course of infection, as observed in ASK cells by DIC ... to induce apoptosis in vivo and in vitro [15-18] Upon activation of the apoptosis machinery, cells undergo distinct morphological and biochemical changes, which include DNA fragmentation and...
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