... study of the literature of the Celts as a means of bridging the ‘‘impassable gulf ’’has for its very interested motive the incorporation of the Irish within the political pale of the United Kingdom.Unsurprisingly, ... for the elimination of difference, whether by trans-forming the Irish or by simply excluding them from the English nation,Arnold presents cultural variety within the United Kingdom, and withinEnglishness ... in the present rather than an ‘‘impassable gulf’’ that separates and divides. In race, in language, in literature, too, ‘‘science exercises the reconciling, the uniting in uence’’ in uncovering...
... found either in wild-type strains and in mutant strains harboring mutations in the genes encoding enzymes of the biosynthesis and/or the transfer of the O-specific polysaccharide [12,13]. The core ... [40–42]. The structure of the inner core region of the LOS fromP. stutzeri OX1, as determined in this study, was found toagree closely with the general structure described above. Itincludes the characteristic ... Y.A.,Zaăhringer, U. & Pier, G. (2003) Elucidation of the structure of the lipopolysaccharide core and the linkage between the core and the O-antigen in Pseudomonas aeruginosa immunotype 5 using...
... 4.2-kbfragment does not contain the gene encoding the deaminasereported here. Inthe cloned 5.2-kb fragment from P. pseudo-alcaligenes JS45, there are no genes involved in the 2-aminophenol-metabolic ... analysis, and the determination of released ammonia. The coupled enzymeassay revealed the mechanism of the deamination reactionand the subsequent metabolism, including the deaminationstep. The product ... enzyme The activity of the enzyme present inthe crude extract ofstrain 10d that used 2 -amino-5-carboxymuconic 6-semi-aldehyde as substrate was measured by monitoring the increase inthe absorbance...
... ubiquitinated inthe presence of the inhibitorof proteasome function. Furthermore, the extent ofubiquitination of TNSALP (1559delT) was furtheraugmented inthe cells expressing ubiquitin, stronglydemonstrating ... Accordingto the recommended nomenclature, the adenine of the initiator ATG codon inthe cDNA of TNSALP isdenoted as nucleotide +1 instead of the first nucleotideof the cDNA clone originally ... became evi-dent inthe medium (Fig. 4B, lane 4), suggesting that the 80 kDa form was processed to the 90 kDa form in the Golgi apparatus before being released into the medium. In support of this,...
... to the a1subunit of PDH1, though it did contain one ADP-binding motif. This suggests the ADP-binding motif in the a1 subunit mediates the observed ATP binding,and FMN is likely situated at the ... that the ADP-binding motif in the b1 subunit mediates FAD binding. On the otherhand, FMN was not detected bound to either subunit(Fig. 5B,C). We also extracted the flavin compoundsfrom the native ... dye-DHs in hyperthermo-philic archaea, including dye-linked d-proline dehy-drogenase [2] and dye-linked l-proline dehydrogenaseKeywordsATP-containing dehydrogenase; dye-linkedL-proline dehydrogenase;...
... the amount of enzyme that unwinds 30% of the DNA helicase substrate at 37 °Cin30min(1 %in one min). For examining the effect of DNA-interactingcompounds on DNA unwinding activity of PDH120, the compounds ... all these inhibitors prior todilution inthe unwinding reaction. Under these condi-tions, there was no inhibition of the unwinding reaction(data not shown). This further confirmed that the inhibition ... were not inhibited by actinomycinC1[22]. Actinomycin C1, a polypeptide containing the properties of an antibiotic, intercalates into dsDNA andthereby inhibits nucleic acid synthesis [23]....
... elongate the poly(A) tail[6,7]. Then, the poly(A) tail binds to the poly(A)-bind-ing protein (PABP), which brings in eIF4G, thusallowing the positioning of the 40s ribosomal subuniton the 5Â-end ... arginine ⁄ glycine-rich(RGG) motifs that are characteristic of RNA-bindingproteins, and an alanine-rich carboxy-terminal sequencethat could be involved in protein–protein interactions.Interestingly, ... is involved in cytoplasmicpolyadenylation in Xenopus oocytes. In this study, a new XGld2-interactingprotein was identified: Xenopus RNA-binding motif protein 9 (XRbm9).This RNA-binding protein...
... 100 100Minus pantoate 2Minus ATP 46 50Minus ATP, minus pantoate 3Minus ATP, minus Pi45Minus ATP, minus ADP 3Eqn (1)Complete system 20Minus pantoate 1Minus ATP NDMinus ATP, minus pantoate ... are able to synthesize panto-thenate de novo, whereas animals rely on pantothenate in their diet.Pantothenate synthetase (PS) catalyzes the last step in the biosynthesis of pantothenic acid, ... pantothenate from the same precursors asbacterial PS but has clearly distinct kinetic properties. The most striking difference is the apparent inhibitionof MM2281 by pantothenate, raising the...
... making their way to the southern shore of Spain during the 1990s. On the other hand, the stories told about these people also depend on the social and political context of the present for their ... that inthe second half of the 1960s, during the comparatively liberal interlude which started in 1966—with the promulgation of the new “Ley de Prensa”—and finished in 1971 the year in which the ... inthe Vth century AD, forming an independent kingdom that lasted until the VIIIth century AD and changing the name of Hispania to Spania inthe process. Thus, each of these terms originally...
... which was indeed predicted. The other maingroup, obtained 97% of the time in 20 740 exploredtrees, contained the DIDO PHD sequences clusteredwith the PHF and the CGBP proteins. As seen in the tree, ... DIO. The GCBP proteins contain a PHD domain, fol-lowed by a DNA-binding domain (the zf-CXXC) and the newly described dPHD region (supplementaryFig. S1). This family is involved in DNA binding ... to maintain transcription. The PHF3 protein was recovered in initial analysesand also contains a TFS2M domain showing the samearchitecture as the DIDO1 long isoform, although lack-ing the dPHD....
... guanine nucleotide releasing protein ND – Rat IGF binding protein 5 ND –Rat FGF receptor activating protein 1 ND –Rat PKC delta-binding protein ND –Rat cellular retinoyl-binding protein ND ... other MACS family proteins. The present studyindicates that this novel protein may play important roles in processing odorants in a zone-specific manner, or the zonalpatterning of the OE during ... MACS family proteins. The recombinant plasmidcarrying either the rat o-macs or the murine MACS1cDNA was transfected into HEK293T cells. The recom-binant proteins contained the c-Myc and His6...