ancient roles in dna replication and genomic stability lead to distinct roles in human disease
Báo cáo y học: "Genomic context analysis in Archaea suggests previously unrecognized links between DNA replication and translation" potx
Ngày tải lên :
14/08/2014, 08:21
... primase DnaG may be involved in DNA replication/ repair in Archaea since the gene encoding DnaG is adjacent to the gene encoding PolB3 in the three crenarchaeal lineages investigated and is located in ... Two main hypotheses can be put forward to explain the genomic association of genes encoding proteins involved in DNA replication and genes coding for proteins involved in translation First, replication ... Materials and methods Identification of DNA replication genes in archaeal genomes A list of 12 factors - corresponding to both monomeric and heteromultimeric proteins - likely to be involved in DNA replication...
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Báo cáo y học: " Initiation of bacteriophage T4 DNA replication and replication fork dynamics: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
Ngày tải lên :
11/08/2014, 21:21
... regions of ssDNA are coated by the single-strand binding protein gp32, UvsY acts as a mediator protein in loading UvsX onto gp32-coated ssDNA, and UvsX is the strandinvasion protein (RecA and Rad51 ... [45,48,52] This inhibitory activity of gp59 presumably acts to prevent the generation of excessive single-stranded DNA and allow coordinated and coupled leading and lagging strand synthesis Unlike ... be within the interior region of a co-infecting T4 DNA molecule, since T4 DNA is also circularly permuted In this way, the process of RDR can in principle initiate soon after an origin-initiated...
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Regulation of spindle behaviour by DNA replication and damage checkpoints in budding yeast
Ngày tải lên :
14/09/2015, 14:03
... and effectors In budding yeast, Mec1, (a phosphatidylinositol kinase like kinase, PIKK and an ortholog of human ATR) is responsible for detection of genomic insults and initiation of signalling, ... phase replication and DNA damage checkpoints, and the G2/M DNA damage checkpoint The G1 and G2 checkpoints respond to some types of localized physical damage to the DNA and are known as DNA damage ... responsible for transporting Esp1 into the nucleus (Ciosk et al., 1998) Thus, up to metaphase, Esp1 is held in check so that the Cohesin complex remains intact and continues to maintain sister-chromatid...
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DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf
Ngày tải lên :
08/03/2014, 19:20
... Chemistry Slovenia Inroduction DNA replication, the process of copying double-stranded DNA, and DNA recombination, the process of breaking DNA strand and joined to a different strand, are central ... progression, DNA replication and DNA repair mechanisms The functioning of replication proteins, recombination proteins, and checkpoint proteins is closely intertwined During DNA replication, stalled ... genome integrity is continuously challenged by DNA lesions, DNA single-strand breaks (SSBs) and double-strand breaks (DSBs) DNA damage and replication checkpoint proteins monitor genome integrity,...
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Báo cáo sinh học: "WordCluster: detecting clusters of DNA words and genomic elements" docx
Ngày tải lên :
12/08/2014, 17:20
... the intersection between the observed and the expected distance distribution The intersection can be interpreted as the point separating the intra-cluster from the inter-cluster distances In this ... [7] to any word or genomic element in the genome, at the same time associating a statistical significance to the clusters found It outperforms current methods relying on densities and sliding-window ... as failures Finally, p is the success probability, i.e the probability to find a target k-mer or genomic element within the DNA sequence Note that in the above equation we use (n-1) instead of...
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Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
Ngày tải lên :
19/02/2014, 18:20
... aAcrystallin requires this domain [66] Attachment of R116C aA-crystallin to Hsp27 and aB-crystallin increases in comparison to wild type, while binding to cC-crystallin and bB2-crystallin decreases ... unfavorable, depending on the disease As a case in point, formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies ... opaque with age and contain many inclusion bodies reactive with antibody to aB-crystallin, but not to b- and c-crystallin, suggesting an important role for aA-crystallin in maintaining lens transparency...
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Báo cáo khoa học: Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategies pot
Ngày tải lên :
06/03/2014, 09:22
... ribonucleoprotein complex normally associated with 5¢ss recognition in the normal splicing process [55] First, U1snRNP binding to an intronic splicing processing element has been found to inhibit pathological ... hindrance of the recruitment of the splicing factors to the targeted splicing competent cis-elements, thus forcing the machinery to use the natural sites Dominski and Kole [61] were the first to ... premRNA splicing In the earliest examples, AONs were aimed at activated cryptic splice sites in the b-globin and CFTR genes in order to restore normal splicing in b-thalassaemia and cystic fibrosis...
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Contrastive analysis on english and vietnames proverbs referring to parts of the human body
Ngày tải lên :
12/12/2013, 00:03
... 3.2 Metonymy in the proverbs referring to parts of the human body 3.3 Metaphor in proverbs referring to parts of the human body 3.4 Personification in proverbs referring to parts of the human ... by the development of technology and information and the exchanges are greatly increasing lead to the demand for learning and teaching of foreign languages and interpretation Contrastive Analysis ... meanings of English proverbs referring to parts of the human body and their Vietnamese equivalents Meaning In the language there are two basic kinds of meaning and they are direct meaning and indirect...
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Báo cáo y học: "Use of soluble MHC class II/peptide multimers to detect antigen-specific T cells in human disease." pps
Ngày tải lên :
09/08/2014, 03:24
... several human MHC class II/peptide staining reagents using transfected Drosophila melanogaster (Schneider, S2) cells [10,12,13] To foster correct HLA-DR (or DQ) αchain and β-chain pairing and protein ... divisions that multimer-staining cells had undergone in culture was estimated using 5-carboxyfluorescein diacetate succinimidyl ester (CFSE) Using multimer and CFSE staining in parallel, Novak et ... combination In studies analyzing antigenspecific T-cell hybridomas, the intensity of binding was shown to be dependent on two main factors: the number of TCRs expressed on the cell surface, and...
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Vai Trò của Thú Nuôi Tiêu Khiển trong Bệnh của Người - Role of Pets in Human Disease
Ngày tải lên :
20/07/2015, 23:42
... thịt ho Hộp tro đựng phân mèo: • Người ta bị nhiễm trùng Toxoplasma nuốt phải ký sinh trùng sau tiếp xúc với phân mèo bị nhiễm trùng Toxoplasmosis phụ nữ có thai gây vấn đề nghiêm trọng cho thai ... biết thêm chi tiết, đọc HealthLinkBC Files sau đây: • #07 Bệnh Dại • #10 Nhiễm Trùng Giardia • #17 Nhiễm Trùng Salmonella • #43 Bệnh Toxoplasma • #48 Nhiễm Trùng Cryptosporidium • #58 Nhiễm Trùng ... Trùng Bao Tử Ruột Muốn biết thêm đề tài HealthLinkBC vào www.HealthLinkBC.ca/healthfiles đến phòng y tế công cộng địa phương quý vị Bấm vào www.HealthLinkBC.ca gọi số 8-1-1 để biết chi tiết dịch...
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Trinucleotide (CAG) repeat polymorphism of the androgen receptor gene in human disease
Ngày tải lên :
11/09/2015, 09:05
... androgen action leading to PCOS in females and high levels of PSA in males Conversely, long CAG tracts were associated with low androgen receptor activity leading to male infertility XXII MAIN ... machinery, coactivators, or other transactivators The domain C named DNA- binding domain (DBD) contains two zinc fingers, which are responsible for DNA recognition and dimerization The domain D ... receptor GTF General transcription factors Gln Glutamine GnRH Gonadotropin-releasing hormone HAT Histone acetyltransferase HD Huntington disease HRE Hormone response elements INSR Insulin receptor...
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Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
Ngày tải lên :
17/03/2014, 03:20
... SV40 and JCV DNA replication using the monopolymerase system The SV40 system contains SV40 TAg and DNA with the SV40 replication origin (A); the JCV system contains JCV TAg and DNA with the JCV replication ... plasmid DNA Nucleosomes may interfere with the initiation of replication and evidence exists that the binding of transcription factors to sites in the regulatory regions adjacent to the replication ... factor such as nuclear factor I (NF-I) required to facilitate initiation of replication in vivo but not in vitro, perhaps by alleviating nucleosome repression or by assisting in origin unwinding...
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Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx
Ngày tải lên :
23/03/2014, 15:20
... either increasing or diminishing their affinity for DNA [42] This ability, to modulate the DNA- binding properties of proteins, was analyzed in relation to the conformational effects of polyamines ... of genomic DNA treated with DNase I Single polyamines or NAPs were allowed to interact with genomic DNA before exposure to phosphodiesterasic endonuclease Assuming that the tested compounds, interacting ... The DNA kb ladder marker was in lane m Fig Electrophoresis of genomic DNA preincubated with single polyamines and then exposed to DNase I (A) Electrophoretic migration, at 37 °C, of genomic DNA...
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Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc
Ngày tải lên :
31/03/2014, 07:20
... efficient binding to the ycf3 promoter as revealed by its almost complete inactivity as a competitor Mutational studies reveal common and distinct DNA- binding properties of SASIG proteins To analyse ... the recombinant SASIG proteins confer promoter-specific binding and transcription initiation on the RNA polymerase, thus providing clues as to their functional roles as sigma factors [19] In the ... HTH-motif [20] in region 4.2 is involved in binding of the )35 promoter element [7] As shown in Fig 7A, two conserved amino acids, E13 and R16, can be identified in all three SASIG proteins within the...
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Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
Ngày tải lên :
11/08/2014, 21:21
... helicase loading protein, the gp61 primase, and the gp32 single stranded DNA binding protein A.) the gp32 single-stranded DNA binding protein binds to regions of displaced DNA near the replication ... backbone to increase stability of the interaction Cooperative binding of ssb proteins assists in unwinding the DNA duplex at replication forks, recombination intermediates, and origins of replication ... holoenzyme have leading and lagging strand synthesis coordinated with a single clamp loader coupled to two DNA polymerases through the τ subunit and to singlestranded DNA binding protein through the...
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Aggregate and spatial distributions of DNA palindromes and their applications to replication origins prediction in some viral genomes
Ngày tải lên :
15/09/2015, 21:52
... provides a binding site for DNA- binding proteins which are often dimeric in structure Such double binding markedly increases the strength and specificity of the binding interaction (Creighton, 1993, ... believed to facilitate the unwinding, in replication origin domains of the genome (Lin et al., 2003) Ribonucleic acid (RNA) is primarily made up of four different bases: adenine, guanine, cytosine, and ... important role in managing, organizing, analyzing and interpreting the rapidly accumulating DNA data Computer algorithms can be used to compare and extract sequence features of interest while...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... excision repair and mismatch repair are down-regulated both in vitro and in vivo Moreover, Hp induces genomic instability in nuclear CA repeats in mice and in mtDNA of AGS cells and chronic gastritis ... Vacuolating cytotoxin in Helicobacter pylori water-soluble proteins upregulates chemokine expression in human eosinophils via Ca2+ influx, mitochondrial reactive oxygen intermediates, and NF-κB ... directly to mtDNA resulting in abnormality in ATP production Therefore, the amount of ATP was further determined aiming to indirectly detect the cell viability and the mitochondrial activity and function...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf
Ngày tải lên :
16/02/2014, 14:20
... ⁄ LEDGF-interacting domain linked to DNA- binding domains (AT-hook and MT domain) becomes disconnected from the PHD domains, the FYRN domain, the transactivating domain, the FYRC domain and the ... apoptotic signaling via competitive binding to death receptors [45] As mentioned above, a second explanation is the inhibitory effect of mostly all GSK3 inhibitors against certain CDKs, including ... Therefore, CDK9 inhibitors may be a promising tool with which to gain insight into the molecular mechanisms of MLLmediated leukemia Moreover, many CDK inhibitors are cross-reactive against glycogen...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
Ngày tải lên :
19/02/2014, 16:20
... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thrombin, dexamethasone, angiotensin II and prostaglandins stimulate ... barbed-end actin and determine the level and distribution of F-actin [34] Therefore, it is tempting to speculate that interactions between monomeric G-actin and actin-binding proteins are a potential ... for 30 and then fixed, permeabilized and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
Ngày tải lên :
07/03/2014, 00:20
... d-fructose, d-galactose, d-sucrose, d-raffinose, d-stachyose, glycine, l-alanine, l-proline, l-lysine, l-serine, b-alanine, taurine, sarcosine, dimethylglycine, glycine betaine, trimethylamine N-oxide, ... can distinctly classify osmolytes into three different classes (a) Class I includes polyhydric alcohols (sorbitol, glycerol, xylitol, adonitol, mannitol) and amino acids and derivatives (glycine, ... (glycine, alanine, proline, serine, lysine, b-alanine and taurine) that have no significant effects on both DGD° and kcat (b) Class II represents methylamines (sarco- Functional stability and activity...
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