analytical aspects of high performance thin layer chromatography

... High- Performance Thin- Layer Chromatography (HPTLC) ManMohan Srivastava Editor High- Performance ThinLayer Chromatography (HPTLC) Editor ManMohan Srivastava Professor Department of Chemistry ... dramatically increased the use of thin- layer chromatography in the form of HPTLC Today, thin- layer chromatography has been successfully hyphenated with high- performance liquid chromatography (HPLC), ... 11 Analytical Aspects of High Performance Thin Layer Chromatography 179 Gunawan Indrayanto 12 Quantitative Analysis and Validation of Method...

Ngày tải lên: 06/10/2016, 09:26

... to measure the amount of RNA used for each RT-PCR reaction Figure Alignment of amino acid sequences of OnTI1, OnTI2 and OnTI3 Comparison of the cDNA amino acid sequences of OnTI1, OnTI2 and OnTI3 ... Page of 14 Figure Characteristic features of organelle markers and subcellular location of proteins of flower-abundant genes in Onc Gower Ramsey A Mitochondrial marker: the first 29 amino acids of ... petals of the A thaliana transformants These Page 10 of 14 results may be due to the absence of a transcription factor that can recognize the Oncidium binding site, highlighting the necessity of...

Ngày tải lên: 11/08/2014, 11:21

... synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosphopantothenate) In a second step, the amide bond of ... analysis of the conditional lethality of the dfp-707 mutant revealed a single point mutation within the N-terminal CoaC domain of Dfp [4] In this study, it is shown that in dfp-1 the residue Ala275 of ... binding one of the (co)substrates of CoaB, indicating that the provided models of the substrate binding sites of human CoaB not give a complete insight into the active-site architecture of CoaB The...

Ngày tải lên: 19/02/2014, 12:20

... pancreatic ducts is of considerable significance in the pathology of a number of diseases including cystic fibrosis and cancer Equally important is the fate of the cytoplasmic tail of the mucins once ... is of particular interest as we, and others, have detected multiple forms of the cytoplasmic tail of MUC1, MUC1FDTR and its derivatives [9,13] Further, the migration of the CT2-reactive forms of ... the possibility of additional cleavage events within the SEA domain that are probably subsequent to that at the G ⁄ SVVV Inspection of the predicted 3D structure of the SEA domain of MUC1 suggests...

Ngày tải lên: 19/02/2014, 18:20

... parts of the Council of Europe For this reason, a Council of Europe consultation was planned, chiefly by means of a survey, with a view to recording the views of as wide a group of children as possible ... how often they had been to see various health care professionals in the last year (1-3 times; 4-6 times; 7-10 times and more often) They were provided with various categories of health care professionals ... aware of the unsatisfactory nature of delay and of cost and that the impact of these practical barriers to child-friendly health needs further study The survey also provides a useful indication of...

Ngày tải lên: 17/03/2014, 17:20

... amount of transcription factors used in (A), with or without lg of IRF-1 or of an IRF3/7 mix and 0.75 lg of CBP; 4X, 2X and 1X correspond to decrease of the amount used in 8X by factors of 2, ... production of IFN and targeting of IRF-3 [45] or IRF-7 [46], the existence of some redundancy in the function of IRF-3 and IRF-7 might help minimize the influence of this later class of virulence ... regions and of ATF-2 for the central region of the coactivators Binding of p50 (amino acids 1–503 of p105) to GST– p300/CBP was very weak overall Binding to CBP–N averaged to 1.5% of input and...

Ngày tải lên: 23/03/2014, 13:20

... steroids and triterpenoids: gravity-flow column liquid chromatography, thin- layer chromatography, gas-liquid chromatography and high- performance liquid chromatography J Chromatogr 452, 377–398 36 Patterson, ... corresponding appearance of label in the medium Most of this label was in the form of GroPCho based on chromatography of the medium on Dowex 50 WX (data not shown) The slow turnover of PtdCho in the ... 2003 Ó FEBS 2003 nucleotides upstream of the STOP codon of the ORF of interest and on the 3¢ end, 65–70 nucleotides of the genomic region downstream of the ORF of interest Transformants were recovered...

Ngày tải lên: 23/03/2014, 18:20

... transformations result from the inactivation or deletion of pro-apoptotic genes or the translocation of an anti-apoptotic gene downstream of highly active promoters [5,7,8] The sigma-2 receptor is ... cytometry Page of (page number not for citation purposes) Journal of Translational Medicine 2009, 7:24 Evaluation of cytotoxicity in vitro Tumor cells were harvested and seeded at a density of approximately ... caspase inhibitor [13] While the anti-tumor effects of sigma-2 ligands alone are modest, the high receptor abundance on cancers and the high affinity of the ligands for the receptor may present a unique...

Ngày tải lên: 18/06/2014, 15:20

... 5Department of Pharmacology, University of Cambridge, Cambridge, UK 6Institute of Neuropathology, University of Magdeburg, Magdeburg, Germany 7Department of Psychiatry, University of Munich, Munich, ... Pembroke College, University of Cambridge, Cambridge, UK 3Institute of Forensic Medicine, Medical University of Gdańsk, Gdańsk, Poland Department of Pharmacology, University of New South Wales, Sydney, ... sACC Figure Illustrations of QUIN-immunoreactive cells from the left sACC of a depressed suicidal patient and a control case and the locations of the analyzed regions of interest (sACC, aMCC and...

Ngày tải lên: 19/06/2014, 22:20

... of the proinflammatory cytokines TNFa and IL6 is significantly attenuated by overexpression of SOD1 With special consideration of the effect of population, the recorded lower concentrations of ... that most of the measured parameters recorded satisfactory values of sensitivity and specificity with the exception of Mg2+ and Na+ which show low specificity values Discussion Protection of the ... concentrations of plasma Ca2+ This could find a support through considering the work of Shearer et al [52] in which they observed lower Ca2+ concentrations in the hair of autistic population and that of...

Ngày tải lên: 19/06/2014, 22:20

... mineralization [1] Page of Figure qRTPCR analysis of FGF-2 regulated genes associated with mineralization; qRTPCR analysis of gene reduction of genes over a 24 hours of treatment with FGF-2 shows ... result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: ... expression of mineralizing genes Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation...

Ngày tải lên: 20/06/2014, 04:20

... mineralization [1] Page of Figure qRTPCR analysis of FGF-2 regulated genes associated with mineralization; qRTPCR analysis of gene reduction of genes over a 24 hours of treatment with FGF-2 shows ... result of a specific sequential regulation of gene expression from the early phase of osteoblast proliferation to the final steps of mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: ... expression of mineralizing genes Hughes-Fulford and Li Journal of Orthopaedic Surgery and Research 2011, 6:8 http://www.josr-online.com/content/6/1/8 Figure qRTPCR analysis of gene induction of proliferation...

Ngày tải lên: 20/06/2014, 07:20

... variations of high- frequency injection pansharpening methods have been proposed as HighPass Filtering Pansharpening and High Pass Modulation As we have already mentioned, the main idea of the high- pass ... integration of Landsat Multispectral Scanner (MSS) and Seasat synthetic aperture radar (SAR) images based on the modulation of the intensity of each pixel of the MSS channels with the value of the ... the launch of the French SPOT satellite system in February of 1986, the civilian remote sensing sector was provided with the capability of applying high- resolution MS imagery to a range of land...

Ngày tải lên: 20/06/2014, 22:20

... quantities of mineralized NH4 -N were higher – than those of NO3 -N in this paper (Table 4), which agreed with the studies of Zhou et al (2001), who indicated that the main source of inorganic ... quantity of CO2-C released in time (days), C0 (CO2-C mg·kg–1 soil) – quantity of soil potential mineralizable C, k (day–1) – constant of C mineralization rate, C0k – potential rate of initial ... significant values of ANOVA analysis (univariate analysis of GLM program) showed that on all days of incubation both the soil moisture and elevation significantly affected the rates of soil C mineralization...

Ngày tải lên: 07/08/2014, 10:21

... occurred there in the same layers within the next IR images of heat field and sap flow of lime 207 Figure Frontal IR-images of the heat field around the linear heater in stem of Tilia_1 for periods ... the outer xylem layers but a little higher in inner xylem layers apparently due to redistribution of flow in xylem It is interesting to compare this situation with the branch cut highly above (around ... be explained as a consequence of redistribution of tensile forces The followed increases of flow could be explained by a decreasing of conducting pathways from side of cutting, drained by the same...

Ngày tải lên: 08/08/2014, 01:22

... studied the effect of a moderate soil drought on the rate of CO assimilation and the amount of soluble and insoluble carbohydrates in leaves of 4-year-old saplings of Q petraea The aim of these experiments ... maintenance of high photochemical efficiency of photosystem II and occurrence of non-uniform CO assimilation Tree Physiol 13, 107-117 Epron D, Dreyer E, Aussenac G (1993) Compared tolerance of photosynthesis ... tolerate long periods of drought The ability of plants to tolerate water deficits has been frequently attributed to their capacity for osmotic adjustment through accumulation of organic compounds...

Ngày tải lên: 08/08/2014, 18:21

... for higher plants This type of data can be useful in planning molecular procedures for DNA polymorphism analysis or the study of genetic architecture of genomes by RFLP and PFGE (choice of rarely ... basis of genome size relative to a standard plant of known ploidy MATERIALS AND METHODS The plant material was leaves of in vitro cloned plantlets of Q petraea (Matt) Liebl, Q pubescens Willd and ... (Provence) Three to four leaves of a single in vitro grown 2-month-old plantlet were chopped with a razor blade together with a leaf fragment of Petunia in mL of Marie’s nuclear isolation buffer...

Ngày tải lên: 08/08/2014, 18:21

... and 1993 Two groups of 10 plants each, chosen from of the species, were droughted for periods of about week at a time during July and August 1992, in a polytunnel Measurements of acoustic emission ... seedlings of the species using the techniques of acoustic emission and hydraulic conductivity It is important to know whether drought susceptibility is inherent as the seedling stage of growth ... using the method of Sperry et al (1988a) Each leaf was cut from its stem under degassed water and its petiole excised from the base of the leaf lamina before cutting to a length of 20 mm All operations...

Ngày tải lên: 08/08/2014, 19:21

... abies INTRODUCTION of heterogeneity in and function of spruce stands within landscapes and along chronosequences and new analytic capabilities to separate the complex effects of multiple factors ... stands of Pinus banksiana were observed in northern Canada [63] Decreases in canopy transpiration of 35 % with aging of Norway spruce were reported by Schubert (in [37]) in a comparison of 40- ... chronosequence of Picea abies stands growing in relatively close proximity within a forested catchment of the Fichtelgebirge, Germany Our purpose was to determine whether regulation of the transpiration...

Ngày tải lên: 09/08/2014, 04:20

... Carvalho is Professor of Economics, Institute of Economics, Federal University of Rio de Janeiro, Brazil Maurizio Caserta is Professor of Macroeconomics at the Faculty of Economics, University of Catania, ... Professor at Queen Mary and Westfield, UK Meghnad Desai is Professor of Economics and Director of the Centre for Study of Global Governance at the London School of Economics Sheila Dow is Professor ... Department at the University of Ottawa Colin Rogers is Associate Professor and Dean of the School of Economics at the University of Adelaide, Australia Malcolm Sawyer is Professor of Economics and Leeds...

Ngày tải lên: 09/08/2014, 19:22