analysis of lady macbeth act 1 scene 5
Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... 6G 155 K, D ( 15 6 15 7), A 158 E 66 ± 12 73 ± 6*M149K, G 155 K, D ( 15 6 15 7), A 158 E 58 ± 5 15 6 ± 13 *N 152 D, G 155 K, D ( 15 6 15 7), A 158 E 62 ± 8 76 ± 7*M149K, N 152 D, G 155 K, D ( 15 6 15 7), A 158 E 93 ± 16 14 8 ± 17 ** Significantly ... given.PAI -1 variant Activity (%) tẵ(min)Wild-type 74 13 63 ± 6M149K 83 ± 11 13 6 ± 24*N 152 D 99 ± 8 67 ± 3G 155 P, K 156 S, G 157 E 75 ± 16 50 ± 2*N 152 D, G 155 P, K 156 S, G 157 E 83 ± 12 67 ± 6G 155 K, D ( 15 6 15 7), ... 4E132A 51 ± 9 30 ± 1* R133A 90 ± 10 57 ± 2R135A 57 ± 6 26 ± 2*F136A 88 ± 12 67 ± 5 I137A 18 ± 2 < ;10 and 17 0 ± 10 *aI138A 52 ± 4 78 ± 4*N139A 7 ± 1 3 35 ± 214 *D140A 63 ± 2 17 ± 0*W141A...
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Numerical analysis of externally prestressed concrete beams part 1
... []{}dKuuKKKSymKKKKKKKKKKKKKKKKKKMQPMQPcccccccccccccccccccccc=⎪⎪⎪⎪⎭⎪⎪⎪⎪⎬⎫⎪⎪⎪⎪⎩⎪⎪⎪⎪⎨⎧⎥⎥⎥⎥⎥⎥⎥⎥⎦⎤⎢⎢⎢⎢⎢⎢⎢⎢⎣⎡=⎪⎪⎪⎪⎭⎪⎪⎪⎪⎬⎫⎪⎪⎪⎪⎩⎪⎪⎪⎪⎨⎧222 1 1 1 66 56 55 46 454 436 353 43326 252 42322 16 1 51 4 1 312 11 222 1 1 1 θνθν (3.24) Every element of matrix [Kc] can be defined as below equations: ))(( 11 11 2 11 jjkkMjNkijkjkcrrqqLEbK ... ⎥⎦⎤+⎟⎠⎞⎜⎝⎛−+−−+⎢⎢⎣⎡+⎟⎠⎞⎜⎝⎛+−⎟⎟⎠⎞⎜⎜⎝⎛+−+−−⎟⎠⎞⎜⎝⎛++=322323322332233223322322 11 66,2 312 , 16 212 12 1, 2 312 12 1 12 1 1xLxLLKxLKxLxLxLKxLxLKLxLKLKxLxLxLKLKLKNvy (3 . 15 ) ⎥⎦⎤⎢⎣⎡−=LxLxNu ,1 (3 .16 ) {}[]2 211 θθvvdTev= ... ⎭⎬⎫⎩⎨⎧+⎭⎬⎫⎩⎨⎧ΔΔ=⎭⎬⎫⎩⎨⎧ΔΔ⎥⎦⎤⎢⎣⎡2 1 2 1 )1( 2 1 )0(22 21 1 211 RRFFUUKKKK (2. 21) Solving the Eq.(2. 21) , we can obtain: {}[]{}{}[]{}()2 211 1 1 111 UKRFKU Δ−+Δ=Δ− (2.22) {}[]{}[]{}{} 211 22222RUKUKF...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... httEx1-25Q-EGFP vector,25QACB 10 004080Counts 12 0 16 0 200 10 1 102FL2-H 10 3 10 425Q+NAC72Q72Q+NAC 10 3Q 10 3Q+NAC04080Counts 12 0 16 0 200 10 0 10 1 102FL2-H 10 3 10 4 10 0 10 1 102FL2-H 10 3 10 404080Counts 12 0 ... 472 44 055 5Tel: +33 472 4326 85 E-mail: arrigo@univ-lyon1.fr(Received 16 February 2006, revised20 April 2006, accepted 12 May 2006)doi :10 .11 11/ j .17 42-4 658 .2006. 05 318 .xWe recently reported that ... NatCell Biol 2, 6 45 652 .79 Grune T, Reinheckel T & Kja D (19 96) Degradation of oxidized proteins in K562 human hematopoietic cells byproteasome. J Biol Chem 2 71, 15 50 4 15 50 9.W. J. J. Firdaus...
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Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf
... 0. 058 ± 0.0 21 0.070 ± 0. 010 b30 .13 6 ± 0.002 0 .12 4 ± 0.0 01 0 .13 7 ± 0.002 0 . 15 3 ± 0.006 0 . 15 4 ± 0.0 05 0 .14 5 ± 0.006/ 1 , ns 0.33 ± 0.08 0 .55 ± 0 .13 0.42 ± 0 .14 0 .50 ± 0 .14 0.63 ± 0.34 0 .53 ± ... HpPex5p + L1 HpPex5p + L2hsi, ns 1. 78 ± 0.04a 1. 40 ± 0.02 1. 31 ± 0.03 1. 43 ± 0.08 1. 31 ± 0.04 1. 25 ± 0. 05 b 1 0.0 35 ± 0.004 0.042 ± 0.008 0.0 31 ± 0.007 0.0 41 ± 0. 011 0.033 ± 0. 012 0.033 ± 0. 012 b20.089 ... (/)ofHpPex5pandHpPex5pcomplexedwithL1orL2atpH7.2andpH 6.0. v2varied between 1. 00 and 1. 05 for all experiments. ns, nanoseconds.pH 7.2 pH 6HpPex5p HpPex5p + L1 HpPex5p + L2 HpPex5p HpPex5p...
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Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... 1. 813 1. 678 1. 6 45 1. 6 45 3 .16 0 3 .16 0Pro12 4.2 95 2.270 1. 990 1. 990 1. 879 3.7 05 3 .5 91 CONH 7.788CONH 7. 017 NHCH 312 3 456 a6bGalNAc 7.803 1. 9 75 4.7 75 4. 016 3. 850 3. 914 3.972 3. 7 15 3.6 91 1446 H. Moăller ... of protons marked by – were not visible.NH abb cc ddÂPro8 4.347 2.397 1. 983 1. 983 1. 983 3.368 3. 317 Asp9 – 4.779 2.747 2 .55 8Thr10 8. 914 4.4 45 4.297 1. 217 Arg 11 8. 51 0 4 .50 4 1. 813 1. 678 1. 6 45 ... s of protonsmarked by were not visible.NH abb cc ddÂPro8 4.347 2.389 1. 978 1. 978 1. 978 3.336 3.336Asp9 4.666 2.707 2 .54 3Thr10 8.386 4. 252 4 . 15 5 1. 134Arg 11 8.484 4 .57 4 1. 798 1. 724 1. 629...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... Septem-ber 20 05, accepted 3 October 20 05) doi :10 .11 11/ j .17 42-4 658 .20 05. 050 00.xBermuda grass pollen (BGP) contains a very complex mixture of allergens,but only a few have been characterized. One of the ... glycoaller-gens. J Biol Chem 2 75, 11 4 51 11 458 .30 Kurosaka A, Yano A, Itoh N, Kuroda Y, Nakagawa T& Kawasaki T (19 91) The structure of a neural specificcarbohydrate epitope of horseradish peroxidase ... Tam MH, Peng HJ, Tsai JJ & HanSH (19 95) Characterization of the isoforms of the groupI allergen of Cynodon dactylon. J Allergy Clin Immunol 95, 12 06 12 14.8 Smith PM, Sulphilglu C, Griffith...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... 0. 05 0.69 ± 0.046 1. 09 1. 51 0 ± 0 .10 1 1. 87 ± 0. 05 1. 24 ± 0.089 0.34W373K 2 .10 ± 0. 21 0.63 ± 0.02 0.30 ± 0.032 0.48 1. 736 ± 0 .12 5 0.22 ± 0. 01 0 .13 ± 0. 011 0.04F461L 0. 65 ± 0. 05 49.27 ± 1. 15 75. 80 ... 42.80 ± 0 .56 62.94 ± 2.89 10 0.00 0 .14 8 ± 0. 013 53 .60 ± 1. 09 362 .16 ± 32 .59 10 0.00F193A 0.0 45 ± 0.00 35 0.22 ± 0.003 4.89 ± 0.39 7.77 0 .12 0 ± 0. 012 1. 29 ± 0.04 10 . 75 ± 1. 13 2.97F200K 3 .50 ± 0.22 ... Institute of Biophysics ASCR, v.v.i., Kra´lovopolska´ 13 5, CZ- 612 65 Brno, Czech RepublicFax: +420 5 41 51 7 18 4Tel: +420 5 41 211 293E-mail: brzoboha@ibp.cz(Received 11 July 2008, revised 17 September...
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Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
... SequenceResiduenumbersS6K1 FDIDL 5 9aS6K2 FDLDL 5 9a4E-BP1 FEMDI 11 4 11 84E-BP2 FEMDI 11 6 12 04E-BP3 FEMDI 86–90HIF1a FVMVL 99 10 3PRAS40 FVMDE 12 9 13 3PKCd FVMEF 4 25 429PKCe FVMEY 484–488STAT3 ... 17 aminoacids [compare the signal for full-length 4E-BP1 (1 11 7) with that for the 18 11 7’ variant] and sections of the C-terminal half of 4E-BP1 [compare, for exam-ple, the 4E-BP1 (98 11 7) ... with 10 0 nm insulinfor 25 min. Cells were lysed in 400 lL of extraction buffercontaining 50 mm b-glycerophosphate (pH 7 .5) , 1 mmEGTA, 1 mm EDTA, 1% (v ⁄ v) Triton X -10 0, 1 mmNa3VO4, 0 .1% ...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... interaction of immobilizedbET -1 with full-length ETB, ETB 13 1and ETB93. (A) Interaction of ETB between 10 and 250 nM. (B) Interaction of ETB 13 1between200 and 16 00 nM. (C) Interaction of ... solubleexpression of GPCRs [ 15 ,22]. In the presence of 1% Brij78 and 1. 5% Brij58, completely soluble ETBcHxwith final yields of c. 3 mg proteinặ(mL RM) )1 wasproduced. With Brij 35 (0 .1% ) and digitonin ... Different distribution of endothelin receptor subtypes in pulmonary tissuesrevealed by the novel selective ligands BQ -12 3 and[Ala 1, 3 ,11 , 15 ]ET -1. Biochem Biophys Res Commun 18 2, 14 4 15 0.40 Saeki T,...
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Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... that like the Trp residue of the0.00 0. 05 0 .10 0 . 15 1. 0 1. 1 1. 2 1. 3[acrylamide] MF0/FFig. 3. Quenching of Trp fluorescence of Ag-NPA -1 by acrylamide.A value of 1. 3M )1 was calculated for the ... the theoretical curve with e ẳ 65% .0.00 0. 25 0 .50 0. 75 1. 000.02 .5 5.07 .5 10 .0oleate [àM]F %Fig. 5. Binding of oleic acid, followed by the enhancement of theTrp fluorescence (DF). The ... nm (eM,2809 .5 Ã 10 3M )1 ặcm )1 for Ag-NPA -1) and the dye absorbances at 337 nm forIAEDANS (eM,3376 Ã 10 3M )1 ặcm )1 ) and at 472 nm forIANBD (eM,47223 Ã 10 3M )1 ặcm )1 ). The covalent...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
... extract 85. 3 3696 0.023 1 100Ammonium sulfate ppt 87.4 51 6 0 .17 7 10 2Phenyl Sepharose CL-4B 71. 9 76 0. 95 41 84BioScale DEAE10 36 .5 17 .4 2 .10 91 43Table 3. Activity of Qor in crude extracts of ... potentials of its redox-activecenters, and first EPR characterization. Biochim. Biophys. Acta 15 44, 15 1 16 5. 14 . Hettrich, D., Peschke, B., Tshisuaka, B. & Lingens, F. (19 91) Themolybdopterin cofactors ... (Uặmg )1 ) Purication (-fold) Yield (%)Crude extract 312 30 45 0 .10 1 100Ammonium sulfate ppt 2 71 416 0. 65 6.4 87Phenyl Sepharose CL-4B 299 78 3.83 37 .5 96BioScale DEAE10 206 9.3 22 .1 216 .7 66 15 72...
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macbeth analysis of fear
... at Lady Macbeth, he constantwashing of her hands, sleepwalking and other behaviour likethis. All done out of fear, and like her husband fear of being caught. Thefinal piece of proof I offer ... common enemy of man, To make themkings, the seeds of Banquo kings! [Act III, S I, L 72- 75] Here Macbeth realizes that if something is not done to Banquo,then his sons will become King. Macbeth ... Thefinal piece of proof I offer is Macbeth& apos;s actions, they were alldue to fear, not only of being caught but of the witches' prophecies, hewas scared of them coming true and tried to...
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ADVANCES IN QUANTITETIVE ANALYSIS OF FINANCE AND ACCOUNTING Volume 1 pdf
... −0.0004 25 Value 14 .7 054 14 . 617 6 14 . 619 9 14 .6 756 14 .6632Error 0.0034 −0.0026 −0.0024 0.0 014 0.00 05 11 0 50 Value 14 . 619 2 14 . 658 3 14 .6734 14 .6662 14 . 656 6 14 . 655 3Error −0.00 25 0.0002 0.0 012 0.0007 ... 0.000000 0 .16 6667 0.666667 0 .16 6667n = 5 0. 013 333 0. 213 334 0 .54 6666 0. 213 334 0. 013 333n = 6 0.003 316 0.0 811 93 0. 4 15 492 0. 4 15 492 0.0 811 93 0.003 316 n = 7 0.000802 0.026 810 0.233 813 0.47 7 15 0 0.233 813 0.026 810 ... 0.026 810 0.000802[W ]w1w2w3w4w5w6w7n=2 1. 000000 1. 000000n=3 1. 7320 51 0.000000 1. 7320 51 n=4 −3.46 410 2 1. 7320 51 0.000000 1. 7320 51 n =5 −2.738608 1. 369304 0.000000 1. 369304 2.738608n=6 −3 .18 9031...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... VRL -1/ 1061F: 5 -ACTCGGTGCTGGAGATCATC-3Â and VRL -1/ 1897R: 5 -TGAGAAGGACGTAGGCCAAC-3Â to obtain fragment C with836 bp; rVRL -11 618 F: 5 -TTCCTGCTGGTCTACCTGGT-3Â and rVRL -12 251 R: 5 -CTTCCTCTGAGGCACTGTTC-3Â ... toAcc. No. AF12 911 3 in overlapping PCR fragments afterreverse transcription of F -11 total RNA (fragment A: 34 56 8 bp ẳ 53 4 bp, fragment B: 54 912 05 bp ẳ 656 bp,fragment C: 10 611 897 bp ẳ 836 bp ... mouse VRL -1 mRNA (Acc. No. NM_ 011 706). Graphi-cal alignment of the open reading frames of rat VRL -1 (Acc.No. AF12 911 3) and mouse VRL -1 (Acc. No. NM_ 011 706)shows that rat and mouse VRL -1 share...
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- analysis of macbeth act 1 scene 5
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