analysis of lady macbeth act 1 scene 5

... 6 G 155 K, D ( 15 6 15 7), A 158 E 66 ± 12 73 ± 6* M149K, G 155 K, D ( 15 6 15 7), A 158 E 58 ± 5 15 6 ± 13 * N 152 D, G 155 K, D ( 15 6 15 7), A 158 E 62 ± 8 76 ± 7* M149K, N 152 D, G 155 K, D ( 15 6 15 7), A 158 E 93 ± 16 14 8 ± 17 * * Significantly ... given. PAI -1 variant Activity (%) t ẵ (min) Wild-type 74 13 63 ± 6 M149K 83 ± 11 13 6 ± 24* N 152 D 99 ± 8 67 ± 3 G 155 P, K 156 S, G 157 E 75 ± 16 50 ± 2* N 152 D, G 155 P, K 156 S, G 157 E 83 ± 12 67 ± 6 G 155 K, D ( 15 6 15 7), ... 4 E132A 51 ± 9 30 ± 1* R133A 90 ± 10 57 ± 2 R135A 57 ± 6 26 ± 2* F136A 88 ± 12 67 ± 5 I137A 18 ± 2 < ;10 and 17 0 ± 10 * a I138A 52 ± 4 78 ± 4* N139A 7 ± 1 3 35 ± 214 * D140A 63 ± 2 17 ± 0* W141A...

Ngày tải lên: 20/02/2014, 11:20

Ngày tải lên: 09/08/2014, 10:22

Ngày tải lên: 11/08/2014, 05:23

Ngày tải lên: 14/08/2014, 09:20

Ngày tải lên: 24/08/2014, 11:05

... [] {} dK u u K KKSym KKK KKKK KKKKK KKKKKK M Q P M Q P c c cc ccc cccc ccccc cccccc = ⎪ ⎪ ⎪ ⎪ ⎭ ⎪ ⎪ ⎪ ⎪ ⎬ ⎫ ⎪ ⎪ ⎪ ⎪ ⎩ ⎪ ⎪ ⎪ ⎪ ⎨ ⎧ ⎥ ⎥ ⎥ ⎥ ⎥ ⎥ ⎥ ⎥ ⎦ ⎤ ⎢ ⎢ ⎢ ⎢ ⎢ ⎢ ⎢ ⎢ ⎣ ⎡ = ⎪ ⎪ ⎪ ⎪ ⎭ ⎪ ⎪ ⎪ ⎪ ⎬ ⎫ ⎪ ⎪ ⎪ ⎪ ⎩ ⎪ ⎪ ⎪ ⎪ ⎨ ⎧ 2 2 2 1 1 1 66 56 55 46 454 4 36 353 433 26 252 42322 16 1 51 4 1 312 11 2 2 2 1 1 1 θ ν θ ν (3.24) Every element of matrix [ K c ] can be defined as below equations: ))(( 11 11 2 11 jjkk M j N k i jkjk c rrqq L Eb K ... ⎥ ⎦ ⎤ + ⎟ ⎠ ⎞ ⎜ ⎝ ⎛ −+−−+ ⎢ ⎢ ⎣ ⎡ + ⎟ ⎠ ⎞ ⎜ ⎝ ⎛ +− ⎟ ⎟ ⎠ ⎞ ⎜ ⎜ ⎝ ⎛ +−+−− ⎟ ⎠ ⎞ ⎜ ⎝ ⎛ + + = 3 2 2 32 3 3 2 23 3 2 2 33 2 2 3 3 2 232 2 11 66 , 2 312 , 16 212 12 1, 2 312 12 1 12 1 1 x L x L L K x L K x L x L x L K x L x L K L x L K L K x L x L x L K L K L K N vy (3 . 15 ) ⎥ ⎦ ⎤ ⎢ ⎣ ⎡ −= L x L x N u ,1 (3 .16 ) {} [] 2 211 θθ vvd T e v = ... ⎭ ⎬ ⎫ ⎩ ⎨ ⎧ + ⎭ ⎬ ⎫ ⎩ ⎨ ⎧ Δ Δ = ⎭ ⎬ ⎫ ⎩ ⎨ ⎧ Δ Δ ⎥ ⎦ ⎤ ⎢ ⎣ ⎡ 2 1 2 1 )1( 2 1 )0( 22 21 1 211 R R F F U U KK KK (2. 21) Solving the Eq.(2. 21) , we can obtain: {} [] {}{} [] {}() 2 211 1 1 111 UKRFKU Δ−+Δ=Δ − (2.22) {} [] {} [] {}{} 211 22222 RUKUKF...

Ngày tải lên: 07/11/2012, 11:04

Ngày tải lên: 26/11/2013, 13:31

... httEx1-25Q-EGFP vector, 25Q AC B 10 0 04080 Counts 12 0 16 0 200 10 1 10 2 FL2-H 10 3 10 4 25Q +NAC 72Q 72Q+NAC 10 3Q 10 3Q+NAC 04080 Counts 12 0 16 0 200 10 0 10 1 10 2 FL2-H 10 3 10 4 10 0 10 1 10 2 FL2-H 10 3 10 4 04080 Counts 12 0 ... 472 44 055 5 Tel: +33 472 4326 85 E-mail: arrigo@univ-lyon1.fr (Received 16 February 2006, revised 20 April 2006, accepted 12 May 2006) doi :10 .11 11/ j .17 42-4 658 .2006. 05 318 .x We recently reported that ... Nat Cell Biol 2, 6 45 652 . 79 Grune T, Reinheckel T & Kja D (19 96) Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome. J Biol Chem 2 71, 15 50 4 15 50 9. W. J. J. Firdaus...

Ngày tải lên: 19/02/2014, 06:20

... 0. 058 ± 0.0 21 0.070 ± 0. 010 b 3 0 .13 6 ± 0.002 0 .12 4 ± 0.0 01 0 .13 7 ± 0.002 0 . 15 3 ± 0.006 0 . 15 4 ± 0.0 05 0 .14 5 ± 0.006 / 1 , ns 0.33 ± 0.08 0 .55 ± 0 .13 0.42 ± 0 .14 0 .50 ± 0 .14 0.63 ± 0.34 0 .53 ± ... HpPex5p + L1 HpPex5p + L2 hsi, ns 1. 78 ± 0.04 a 1. 40 ± 0.02 1. 31 ± 0.03 1. 43 ± 0.08 1. 31 ± 0.04 1. 25 ± 0. 05 b 1 0.0 35 ± 0.004 0.042 ± 0.008 0.0 31 ± 0.007 0.0 41 ± 0. 011 0.033 ± 0. 012 0.033 ± 0. 012 b 2 0.089 ... (/)ofHpPex5pandHpPex5pcomplexedwithL1orL2atpH7.2and pH 6.0. v 2 varied between 1. 00 and 1. 05 for all experiments. ns, nanoseconds. pH 7.2 pH 6 HpPex5p HpPex5p + L1 HpPex5p + L2 HpPex5p HpPex5p...

Ngày tải lên: 21/02/2014, 00:20

... 1. 813 1. 678 1. 6 45 1. 6 45 3 .16 0 3 .16 0 Pro12 4.2 95 2.270 1. 990 1. 990 1. 879 3.7 05 3 .5 91 CONH 7.788 CONH 7. 017 NHCH 312 3 456 a6b GalNAc 7.803 1. 9 75 4.7 75 4. 016 3. 850 3. 914 3.972 3. 7 15 3.6 91 1446 H. Mo ă ller ... of protons marked by – were not visible. NH abb cc dd Pro8 4.347 2.397 1. 983 1. 983 1. 983 3.368 3. 317 Asp9 – 4.779 2.747 2 .55 8 Thr10 8. 914 4.4 45 4.297 1. 217 Arg 11 8. 51 0 4 .50 4 1. 813 1. 678 1. 6 45 ... s of protons marked by were not visible. NH abb cc dd Pro8 4.347 2.389 1. 978 1. 978 1. 978 3.336 3.336 Asp9 4.666 2.707 2 .54 3 Thr10 8.386 4. 252 4 . 15 5 1. 134 Arg 11 8.484 4 .57 4 1. 798 1. 724 1. 629...

Ngày tải lên: 21/02/2014, 15:20

... Septem- ber 20 05, accepted 3 October 20 05) doi :10 .11 11/ j .17 42-4 658 .20 05. 050 00.x Bermuda grass pollen (BGP) contains a very complex mixture of allergens, but only a few have been characterized. One of the ... glycoaller- gens. J Biol Chem 2 75, 11 4 51 11 458 . 30 Kurosaka A, Yano A, Itoh N, Kuroda Y, Nakagawa T & Kawasaki T (19 91) The structure of a neural specific carbohydrate epitope of horseradish peroxidase ... Tam MH, Peng HJ, Tsai JJ & Han SH (19 95) Characterization of the isoforms of the group I allergen of Cynodon dactylon. J Allergy Clin Immunol 95, 12 06 12 14. 8 Smith PM, Sulphilglu C, Griffith...

Ngày tải lên: 07/03/2014, 12:20

... 0. 05 0.69 ± 0.046 1. 09 1. 51 0 ± 0 .10 1 1. 87 ± 0. 05 1. 24 ± 0.089 0.34 W373K 2 .10 ± 0. 21 0.63 ± 0.02 0.30 ± 0.032 0.48 1. 736 ± 0 .12 5 0.22 ± 0. 01 0 .13 ± 0. 011 0.04 F461L 0. 65 ± 0. 05 49.27 ± 1. 15 75. 80 ... 42.80 ± 0 .56 62.94 ± 2.89 10 0.00 0 .14 8 ± 0. 013 53 .60 ± 1. 09 362 .16 ± 32 .59 10 0.00 F193A 0.0 45 ± 0.00 35 0.22 ± 0.003 4.89 ± 0.39 7.77 0 .12 0 ± 0. 012 1. 29 ± 0.04 10 . 75 ± 1. 13 2.97 F200K 3 .50 ± 0.22 ... Institute of Biophysics AS CR, v.v.i., Kra ´ lovopolska ´ 13 5, CZ- 612 65 Brno, Czech Republic Fax: +420 5 41 51 7 18 4 Tel: +420 5 41 211 293 E-mail: brzoboha@ibp.cz (Received 11 July 2008, revised 17 September...

Ngày tải lên: 16/03/2014, 04:20

... Sequence Residue numbers S6K1 FDIDL 5 9 a S6K2 FDLDL 5 9 a 4E-BP1 FEMDI 11 4 11 8 4E-BP2 FEMDI 11 6 12 0 4E-BP3 FEMDI 86–90 HIF1a FVMVL 99 10 3 PRAS40 FVMDE 12 9 13 3 PKCd FVMEF 4 25 429 PKCe FVMEY 484–488 STAT3 ... 17 amino acids [compare the signal for full-length 4E-BP1 (1 11 7) with that for the 18 11 7’ variant] and sections of the C-terminal half of 4E-BP1 [compare, for exam- ple, the 4E-BP1 (98 11 7) ... with 10 0 nm insulin for 25 min. Cells were lysed in 400 lL of extraction buffer containing 50 mm b-glycerophosphate (pH 7 .5) , 1 mm EGTA, 1 mm EDTA, 1% (v ⁄ v) Triton X -10 0, 1 mm Na 3 VO 4 , 0 .1% ...

Ngày tải lên: 16/03/2014, 06:20

... interaction of immobilized bET -1 with full-length ETB, ETB 13 1 and ETB 93 . (A) Interaction of ETB between 10 and 250 n M. (B) Interaction of ETB 13 1 between 200 and 16 00 n M. (C) Interaction of ... soluble expression of GPCRs [ 15 ,22]. In the presence of 1% Brij78 and 1. 5% Brij58, completely soluble ETB cHx with final yields of c. 3 mg proteinặ(mL RM) )1 was produced. With Brij 35 (0 .1% ) and digitonin ... Different distribution of endothelin receptor subtypes in pulmonary tissues revealed by the novel selective ligands BQ -12 3 and [Ala 1, 3 ,11 , 15 ]ET -1. Biochem Biophys Res Commun 18 2, 14 4 15 0. 40 Saeki T,...

Ngày tải lên: 16/03/2014, 10:20

... that like the Trp residue of the 0.00 0. 05 0 .10 0 . 15 1. 0 1. 1 1. 2 1. 3 [acrylamide] M F 0 /F Fig. 3. Quenching of Trp fluorescence of Ag-NPA -1 by acrylamide. A value of 1. 3 M )1 was calculated for the ... the theoretical curve with e ẳ 65% . 0.00 0. 25 0 .50 0. 75 1. 00 0.0 2 .5 5.0 7 .5 10 .0 oleate [àM] F % Fig. 5. Binding of oleic acid, followed by the enhancement of the Trp fluorescence (DF). The ... nm (e M,280 9 .5 Ã 10 3 M )1 ặcm )1 for Ag-NPA -1) and the dye absorbances at 337 nm for IAEDANS (e M,337 6 Ã 10 3 M )1 ặcm )1 ) and at 472 nm for IANBD (e M,472 23 Ã 10 3 M )1 ặcm )1 ). The covalent...

Ngày tải lên: 16/03/2014, 18:20

... extract 85. 3 3696 0.023 1 100 Ammonium sulfate ppt 87.4 51 6 0 .17 7 10 2 Phenyl Sepharose CL-4B 71. 9 76 0. 95 41 84 BioScale DEAE10 36 .5 17 .4 2 .10 91 43 Table 3. Activity of Qor in crude extracts of ... potentials of its redox-active centers, and first EPR characterization. Biochim. Biophys. Acta 15 44, 15 1 16 5. 14 . Hettrich, D., Peschke, B., Tshisuaka, B. & Lingens, F. (19 91) The molybdopterin cofactors ... (Uặmg )1 ) Purication (-fold) Yield (%) Crude extract 312 30 45 0 .10 1 100 Ammonium sulfate ppt 2 71 416 0. 65 6.4 87 Phenyl Sepharose CL-4B 299 78 3.83 37 .5 96 BioScale DEAE10 206 9.3 22 .1 216 .7 66 15 72...

Ngày tải lên: 17/03/2014, 10:20

Ngày tải lên: 21/03/2014, 22:01

... at Lady Macbeth, he constant washing of her hands, sleepwalking and other behaviour like this. All done out of fear, and like her husband fear of being caught. The final piece of proof I offer ... common enemy of man, To make them kings, the seeds of Banquo kings! [Act III, S I, L 72- 75] Here Macbeth realizes that if something is not done to Banquo, then his sons will become King. Macbeth ... The final piece of proof I offer is Macbeth& apos;s actions, they were all due to fear, not only of being caught but of the witches' prophecies, he was scared of them coming true and tried to...

Ngày tải lên: 21/03/2014, 22:08

... −0.0004 25 Value 14 .7 054 14 . 617 6 14 . 619 9 14 .6 756 14 .6632 Error 0.0034 −0.0026 −0.0024 0.0 014 0.00 05 11 0 50 Value 14 . 619 2 14 . 658 3 14 .6734 14 .6662 14 . 656 6 14 . 655 3 Error −0.00 25 0.0002 0.0 012 0.0007 ... 0.000000 0 .16 6667 0.666667 0 .16 6667 n = 5 0. 013 333 0. 213 334 0 .54 6666 0. 213 334 0. 013 333 n = 6 0.003 316 0.0 811 93 0. 4 15 492 0. 4 15 492 0.0 811 93 0.003 316 n = 7 0.000802 0.026 810 0.233 813 0.47 7 15 0 0.233 813 0.026 810 ... 0.026 810 0.000802 [W ]w1w2w3w4w5w6w7 n=2 1. 000000 1. 000000 n=3 1. 7320 51 0.000000 1. 7320 51 n=4 −3.46 410 2 1. 7320 51 0.000000 1. 7320 51 n =5 −2.738608 1. 369304 0.000000 1. 369304 2.738608 n=6 −3 .18 9031...

Ngày tải lên: 23/03/2014, 12:20