analysis of embryonic stem cell derived osteogenic cultures pdf

... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... structures of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C-cells of the thyroid ... µm 20 µm Analysis the vascular and haematopoietic cell gene markers in the BMP2+ cell population Figure of Analysis of the vascular and haematopoietic cell gene markers in the BMP2+ cell population...

Ngày tải lên: 14/08/2014, 08:20

... Hescheler J, Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic research ... lists of transcripts (a) The pie chart shows the distribution of these annotations (b) The bar chart shows the number of genes in the categories adhesion, cell cycle, cell death, cell- cell signalling, ... developed remarkable mechanisms of cytoprotection and cell survival, also because of the absence of cell division [27] reports Gene Ontology enrichment analysis of the downregulated genes in α-MHC+...

Ngày tải lên: 14/08/2014, 20:22

... Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable and unlimited source of ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... Chapter 1: Introduction 1.1 Neural stem cells 1.1.1 A brief history of NSCs The field of neural stem cell (NSC) biology has its humble beginnings in the second half of the 19th century Several published...

Ngày tải lên: 10/09/2015, 09:08

... for a role in ES cell self renewal Of primary interest are genes whose expressions are restricted to the embryonic stages, as pluripotency is characteristic of embryo derived stem cells The similarity ... seeding densities of 300 and 500 cells per 56cm2 (surface area of each well of a well dish is 56cm2) When 300 cells were reseeded, the number of secondary colonies formed for cells transfected ... cells Put together, all these evidence suggest that the regulation of Lefty1 by OCT4, KLF4 and NANOG may be part of the ES cell self renewal mechanism The role of Lefty1 in regulation of ES cell...

Ngày tải lên: 11/09/2015, 16:02

... embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated A part of ... role(s) in embryonic stem cells To elucidate the biological role(s) of Lefty2, loss of function studies using shRNAs was carried out I was able to show that Lefty2 RNAi induces ES cell differentiation ... specification of ES cells is a SMAD3 dependent process On the other hand, SMAD3 plays no role in trophectodermal 158 specification of ES cells as inhibition of SMAD3 activity does not lead to ES cell...

Ngày tải lên: 11/09/2015, 16:02

... ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For transfection of ... and analysis was carried out on the fourth day of selection 4.9 Alkaline phosphatase staining Detection of alkaline phosphatase, which is indicative of the nondifferentiated state of ES cells, ... 4.14.1 Enhancement of Nodal/Activin signaling E14, TAG1 or tet-Nodal E14tg2a cells were seeded in ES cell medium at a density of 676 cells/cm2 without gelatin The next day, the seeded cells were subjected...

Ngày tải lên: 11/09/2015, 16:02

... pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells ... expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self-renewal of teratocarcinoma and embryonic stem cells Oncogene ... fails to maintain self-renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells enables feeder-free growth...

Ngày tải lên: 11/09/2015, 16:02

... 1.1 Embryonic stem cells 1.1.1 Derivation and definition of embryonic stem cells During the transition of an embryo from the morula to the blastocyst ... inefficient clonal propagation etc with stem cells derived from post implantation mouse epiblasts Human ES cells and mouse epiblast derived stem cells also share patterns of gene expression and signaling ... lines of evidence that Nodal signaling mediated by SMAD2/3 indeed plays a role in self renewal and cell fate decisions of pluripotent cell populations in vivo and also that of embryonic stem cells...

Ngày tải lên: 11/09/2015, 16:02

... Subunit of Cadherin protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone H3 methyltransferase Transcription factor Intracellular mediator of canonical ... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells and differentiated cells...

Ngày tải lên: 11/09/2015, 16:03

... Maintenance of pluripotential embryonic stem cells by stem cell selection Reprod Fertil Dev 1998, 10:527-533 53 Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonic stem cells and of ... differentiation of embryonic stem cells Curr Opin Cell Biol 1995, 7:862-869 10 Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, Ailhaud G: Differentiation of embryonic stem cells ... the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose stem cell biology and their...

Ngày tải lên: 09/08/2014, 20:22

... is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation ... methylation profile of the human embryonic stem cell line HSF1 [22] BS-seq is able to generate genome-wide DNA methylation profiles at single base resolution, much improved from previous profiling ... for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA SF is Special Fellow of the Leukemia...

Ngày tải lên: 09/08/2014, 23:20

... totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) are routinely derived from the inner cell mass of blastocysts and represent pluripotential embryonic ... mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10(910):1518-1525 Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse ... in mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation...

Ngày tải lên: 12/08/2014, 01:22

... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors ... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... Endothelial cells derived from human embryonic stem cells Proc Natl Acad Sci USA 2002, 99:4391-4396 Choi K: The hemangioblast: a common progenitor of hematopoietic and endothelial cells J Hematother Stem...

Ngày tải lên: 14/08/2014, 08:20

... mediated by NSC -derived neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex of E16 Sprague-Dawley ... of the differentiation of forelimb and foot faults (DOFFF) derived from ischemic control mice over four weeks compared to the sham-operated normal control rats The DOFFF of the three groups of ... ventricles of ischemic rats with and without NGF administration or neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic...

Ngày tải lên: 18/06/2014, 16:20

... CD: Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency Stem Cells 2005, 23:663-680 35 Munne S, Howles CM, Wells D: The role of preimplantation ... Page of 18 onic stem (mES) cell clones capable of the inducible overexpression of each one of 32 selected genes, 29 murine orthologs of HSA21 genes and HSA21 coding sequences, under the control of ... overexpression of some of the HSA21 genes is due to a high basal expression level of these genes Dosage sensitivity of HSA21 genes in mES cells We further investigated the cause of the lack of a strong...

Ngày tải lên: 09/08/2014, 20:22

... Derivation of DCs from hES cells Derivation of DCs from hES cells: Undifferentiated hES cells were cocultured with S17 mouse stromal cells to derive cystic bodies Later, purified CD34+ cells derived ... derivation of macrophagres from hES-CD34+ cells whereas studies of Galic et al [20] derived functional T cells from hES-CD34+ cells in vivo using humanized mice Whether the hES derived T cells support ... assay The T cell stimulatory capacity of DCs derived from hES cells CD34+ progenitor cells was assessed by co-incubating graded numbers of CD1a+ cells previously sorted on the basis of CD1a immunomagnetic...

Ngày tải lên: 10/08/2014, 05:20

... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell ... obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos ... Cosponsor the "Stem Cell Research Enhancement Act" [letter to Members of the Congress of the United States] January 19, 2005 Fact Sheet -Embryonic Stem Cell Research U.S Department of Health & Human...

Ngày tải lên: 13/08/2014, 23:20

... to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/ progenitor cells Stem Cells 2006, 24:889-895 NIA array analysis [http://lgsun.grc.nia.nih.gov/ANOVA/ ... al cells Mol Cell Biol 2006, 26:3514-3526 Ohnishi S, Yasuda T, Kitamura S, Nagaya N: Effect of hypoxia on gene expression of bone marrow -derived mesenchymal stem cells and mononuclear cells Stem ... Background Multiple types of adult stem cell exist, of which the hematopoietic stem cell (HSC), which gives rise to cells of all hematopoietic lineages for the life of an animal, is the best...

Ngày tải lên: 14/08/2014, 08:20

... application of the method produces a set of 4,449 cell and tissue markers, including 45 out of 71 known stem cell markers (69%) Analysis of the markers that segregate six types of stem cells (hematopoietic, ... differentiated sets of stem cells We chose six pairs of stem cell samples and their differentiated derivatives from our dataset (two embryonic stem cell lines, hematopoietic stem cells [HSCs], osteoblasts, ... function that endows cells with the property of 'stemness', in the same way that there appears to be no single stemness gene for all stem cells [60] This set of 426 markers of stem cell differentiation...

Ngày tải lên: 14/08/2014, 08:20

... range of threshold values Embryonic stem (ES) cells are derived from the inner cell mass of the embryo and possess the property of pluripotency, which is the ability to develop into any cell lineage ... circuitry in human embryonic stem cells Cell 2005, 122:947-956 Kim J, Chu J, Shen X, Wang J, Orkin SH: An extended transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132:1049-1061 ... which a stem cell maintains its identity, and how it can be directed to form different cell types Our work will allow for a more detailed examination of the components of this expanded stem cell...

Ngày tải lên: 14/08/2014, 20:22