an anthocyanin specific membrane transporter

... with mouse anti-human Golgin 97 (A) serum, mouse anti-human LAMP2 serum (D), mouse anti-human CD63 serum (G), mouse anti-human MPR serum (J) or mouse anti-human EEA1 serum (M) Primary antibodies ... region–transmembrane domain–RING-H2 motif protein (AtRMR1) was found to colocalize with a protein storage vacuole membrane marker and was predicted to be a receptor mediating targeting to the plant ... detected with rabbit anti-FLAG serum and, as secondary antibody, anti-rabbit Texas Red serum Cells were counterstained with mouse antibodies raised against PDI (E) and donkey anti-rabbit Alexa Fluor...

Ngày tải lên: 18/02/2014, 08:20

... kDa) or two copies each of the membrane subunit and LolD (molecular mass ¼ 138–142 kDa), although an ABC transporter is generally composed of two membrane domains and two nucleotide-binding domains ... (Figs and 4) On the other hand, various membrane apparatuses, including ABC transporters such as maltose permease [21,23], histidine permease [24] and the LolCDE complex [7], and a Sec protein translocase ... Tokuda H (2003) A mutation in the membrane subunit of an ABC transporter LolCDE complex causing outer membrane localization of lipoproteins against their inner membrane- specific signals Mol Microbiol...

Ngày tải lên: 19/02/2014, 00:20

... weak acid-inducible ABC transporter Pdr12 transports fluorescein and preservative anions from the cytosol by an energy-dependent mechanism J Bacteriol 181, 4644–4652 Hatzixanthis, K., Mollapour, ... former mutant is more sensitive to acetate at pH 4.5 [2] Another study reported that Azr1p, a plasma membrane transporter of the major facilitator superfamily, also confers acetate resistance [12] ... acetate (n) and 80 mM acetate (m) The cultures in (A) and (B) were grown in the absence of any medium tryptophan supplement, whereas the cultures in (C) and (D) were grown with a 50 mM tryptophan supplementation...

Ngày tải lên: 23/03/2014, 21:20

... Pharmacogenomics of Membrane Transporters 1 Pharmacogenomics of Membrane Transporters An Overview Qing Yan Introduction 1.1 Membrane Transporters: Essential for Normal Physiological Functions Membrane transporters ... Based on mechanisms and energetics, membrane transporters can be categorized into two broad classes: passive transporters and active transporters Passive transporters include ion channels, such ... distribution can also be influenced by membrane transporters Nucleosides and their analogs including antivirals and antineoplastics depend on specific transporters to reach their target sites Transporters...

Ngày tải lên: 11/04/2014, 09:50

... inflammatory cells, and human herpesvirus in human salivary glands J Histochem Cytochem 2004, 52:671-681 Umehara H, Tanaka M, Sawaki T, Jin ZX, Huang CR, Dong L, Kawanami T, Karasawa H, Masaki ... necrosis factor-α and granulocyte monocyte colony stimulating factor production by NKcells and other cell subsets that have been suggested to play a role in the initiation phase and pathogenesis ... administration of anti-fractalkine antibodies ameliorated experimental RA [9] With regard to diseases involving the kidneys, a viral fractalkine antagonist reduced kidney inflammation and proteinurea...

Ngày tải lên: 09/08/2014, 10:23

... prostate-associated antigens such as the secreted protease prostatespecific antigen (PSA) and the highly specific membrane antigen present in their plasma membrane, prostatespecific membrane antigen (PSMA) ... prostate cancer: a new alternatively spliced variant of prostate -specific membrane antigen Prostate 2007, 67:1791-1800 12 Lapidus RG, Tiffany CW, Isaacs JT, Slusher BS: Prostate -specific membrane antigen ... is elevated in prostate cancer cells Prostate 2000, 45:350-354 13 Anilkumar G, Rajasekaran SA, Wang S, Hankinson O, Bander NH, Rajasekaran AK: Prostate -specific membrane antigen association with...

Ngày tải lên: 10/08/2014, 10:20

... figure 1B, with at least 15 transmembrane helixes, two-halves of transmembrane helixes and a TMD0 of at least transmembrane spans AtMRP6 can be expressed in mammalian cells but not in yeast In ... of the protein exhibits only transmembrane helixes whereas transmembrane spans are usually found HMMTop_V2 (very well-known and suitable for the analysis of ABC transporters) as well as Phobius, ... resistance Plant J 2007, 50:207-218 Higgins CF: ABC transporters: from microorganisms to man Annu Rev Cell Biol 1992, 8:67-113 Van Veen HW, Konings WN: Multidrug transporters from bacteria to man: similarities...

Ngày tải lên: 12/08/2014, 05:20

... ions in animal cells [4,21] Conversely, mutants resistant to nickel were significantly more abundant than cadmium-resistant mutant strains More than two-thirds of the nickel-resistant mutants were ... Other transporter mutants exhibiting cadmium (but not nickel) resistance include smf2Δ, an intracellular manganese transporter [52] (see also Figure 7), and zrt3Δ, which is a transporter that mobilizes ... downregulation of plasma membrane receptors and transporters, localization and processing of vacuolar components, and removal of selected regions of the plasma membrane, coupled with ingestion...

Ngày tải lên: 14/08/2014, 08:21

... phân hủy anthocyanin nước giải khát có CO2 phối màu với anthocyanin từ xác nho Macccarone cộng nghiên cứu quang hóa anthocyanin , anthocyanin diglycosyl hóa vị trí C3 C-5 bền anthocyanin mono ... lượng Anthocyanin nồng độ SƠ2 khác 20 Hình 24 Ket hàm lượng Anthocyanin nhiệt độ khác n h au 20 I Tổng quan Anthocyanin: Giói thiệu: Các Anthocyanin thuộc nhóm chất màu tự nhiên tan nước ... chất Anthocyanin: Anthocyanin tỉnh khiết dạng tinh thể vô định hình họp chất phân cực nên tan tốt dung môi phân cực Anthocyanin hòa tan tốt nước dịch bão hòa Khi kết hợp với đường cho phân tử anthocyanin...

Ngày tải lên: 22/09/2016, 23:13

... Pelargonidin-Based Anthocyanins, J.Agric and Food Chemistry, Vol 47, No.11, 1999 [4] Fuleki, T., Francis, F.J., Quantitative Methods for Anthocyanins Determination of total anthocyanin and degradation ... 4,5 a: Lng anthocyanin, g; M: Khi lng phõn t ca anthocyanin, g/mol; l: Chiu dy cuvet, cm; K: pha loóng; V: Th tớch dch chit, l T ú tớnh c hm lng anthocyanin theo phn trm: % Anthocyanin ton phn ... 510ữ540nm hp th l yu t liờn quan mt thit n mu sc ca cỏc anthocyanin chỳng ph thuc vo pH ca dung dch, nng anthocyanin: thng pH thuc vựng acid mnh cú hp th ln, nng anthocyanin cng ln hp th cng mnh...

Ngày tải lên: 19/03/2013, 11:12

Ngày tải lên: 29/01/2014, 14:35

... antibody, after which the membrane was stripped and reprobed with an anti-actin antibody Top panel, anti-DAT immunoreactivity; molecular size markers indicated on the left Lower panel, actin immunoreactivity ... DAT DAT belongs to the family of neurotransmitter transporters that includes the norepinephrine and serotonin transporters (NET and SERT), which share a range of structural characteristics The ... peroxidase-conjugated secondary anti-goat and anti-rabbit antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) The monoclonal mouse anti-b-actin antibody (clone AC15) was purchased...

Ngày tải lên: 14/02/2014, 21:20

... ankyrin B plays an important role in the membrane- blebbing process [19], DAPK-1 and s-DAPK-1 may be able to interact with ankyrin B via their ankyrin repeats and thus promote membrane blebbing Although ... PAGE and detection by immunoblotting were done as previously described [2] The following antibodies were used: anti-HA (Covance, Princeton, NJ, USA), anti-GST and anti-Flag (Sigma), and anti-PARP ... biological significance of the s-DAPK-1- and DAPK-1-induced membrane blebbing requires further investigation Experimental procedures Cell culture and harvest, plasmids and transfection, and treatment...

Ngày tải lên: 18/02/2014, 17:20

... per lane), transferred onto nitrocellulose membrane and subjected to direct immunoblot analysis using rabbit anti-(LBP Ig) as the primary probe Lane 1, cell supernatant; lane 2, cell pellet; lanes ... nitrocellulose membrane and subjected to Western blot analysis using anti-LBP Ig, a single band at 67 kDa region was obtained (Fig 2, lane 2), suggesting LBP to be one of the membrane proteins of Leishmania ... either anti-LBP IgG or anti-laminin IgG Proteolytic digestion of L donovani membrane L donovani membrane preparations were made according to the method described previously [15] Membrane preparations...

Ngày tải lên: 21/02/2014, 00:20

... GTP-binding proteins such as Ras, Ran, Rab, Rap and Rho, which exhibit vibrational uncoupling of the phosphate resonances and signicant shifts of the a, b and c absorbance bands, resulting from strong ... intermediate I and Pi are proportional to the absorption changes at 1255 cm)1 (vas a-b-ATP band), 1338 cm)1 (unidentied protein side chain band) and 1078 cm)1 (inorganic phosphate band), respectively ... scans) and 6001000 s (7172; 1000 scans) The averaged interferograms were manipulated by zero lling using a factor of 2, and Fourier-transformed using Mertz phase correction and the BlackmanHarris...

Ngày tải lên: 07/03/2014, 00:20

... interact with a membrane receptor and activate an intracellular signal transduction cascade in a manner analogous to TNF-a Chromogranins/secretogranins The granin family comprises another example ... ATP-binding cassette A1 (ABCA1) transmembrane lipid transporter, which is responsible for the transfer of phospholipid from cell membranes to circulating HDL [40,41] Notably, in Tangier disease, ABCA1 activity ... regulation of proliferation and survival), Janus-activated kinase 2, signal transducer and activator of transcription (which is recognized as an oncogene implicated in many cancers) and extracellular-signal...

Ngày tải lên: 07/03/2014, 05:20

... evolutionary origin and folding, even though sequence identity between many members cannot be detected anymore Most of the transporters in ST3 transport organic and inorganic anions while a smaller ... re-entrant loops in ST3 transporters seems likely as mutagenesis experiments have shown that re-entrant loop Xa in the citrate transporters CitS and CitP and the malate transporters MleP and CimH in [st326]2HCT ... (2004) Sequence and hydropathy profile analysis of two classes of secondary transporters Mol Membr Biol, in press Bandell M, Ansanay V, Rachidi N, Dequin S & Lolkema J (1997) Membrane potential...

Ngày tải lên: 07/03/2014, 17:20

... plasma membrane ATP-binding cassette (ABC) transporter determinants of drug resistance (Pdr5p and Snq2p [46]) were streaked onto plates containing GA and RD This revealed the Dpdr5 mutant to ... RD and GA sensitivities of strains bearing deletions of the Pdr5 and Snq2 plasma membrane ABC transporters Wild-type cells (wt), Dpdr5 or Dsnq2 single mutants, and a Dpdr5,Dsnq2 double mutant, ... sensitizing yeast to Hsp90 inhibitor antibiotics (Eur J Biochem 270) 4693 Fig Analysis of cochaperone and ABC transporter mutants for Hsp90 drug sensitivity (A) RD and GA sensitivities of strains...

Ngày tải lên: 07/03/2014, 21:20

... the membrane [38] and sphingolipid is essential for retention of the protein in the plasma membrane [39] There is also a strong possibility that the lipid anchors that tether proteins to membrane ... FEBS 4695 Membrane raft structure P J Quinn and C Wolf Laboratory Drs Galya Staneva and Lin Chen are thanked for providing assistance in preparing samples The work was aided by a grant of beamtime ... domains in living cell membranes is defined as between 10 and 200 nm [32], and vesicles derived from rafts occupying areas of the parent membrane of this order would be between and 30 nm in diameter...

Ngày tải lên: 15/03/2014, 23:20

... (2002) An approach to membrane protein structure without crystals Proc Natl Acad Sci USA 99, 14037–14040 24 Li, H., Li, F., Sun, H & Qian, Z.M (2003) Membrane- inserted conformation of transmembrane ... peptide is highly hydrophobic, and insoluble in water and a range of organic solvents We have chosen TFE and SDS to solubilize the peptide and to mimic biological membranes The peptide is stable ... judging from both angular order parameters ( 1.0) for backbone / and w dihedral angles and Ramachandran space analysis from PROCHECK-NMR When the structures of DMT1-TM4 in both SDS and TFE were superimposed...

Ngày tải lên: 16/03/2014, 16:20