amplification cloning and sequencing of the srv 2 env genes
Báo cáo y học: "Broad-range PCR, cloning and sequencing of the full 16S rRNA gene for detection of bacterial DNA in synovial fluid samples of Tunisian" pps
Ngày tải lên :
09/08/2014, 14:22
... used the broad-range PCR, cloning and sequencing the entire 16S rDNA and demonstrated the presence of a large number of bacterial DNA in the synovial tissue (ST) of patients with ReA, UA, and other ... 30 M Sexually acquired ReA 2. 4 (1 to 6) Clinical details UA (n = 9) 25 (2 to 60) 40 (22 to 59) 5:4 - RA (n = 7) 66 ( 12 to 22 8) 44 (39 to 53) 2: 5 - OA (n = 6) 14 ( 12 to 24 ) 58 (44 to 70) 5:1 - a ... PCR amplification system PCR was positive in 20 of the 27 (74.10%) patients indicating the presence of bacterial 16S rDNA within the synovial samples Of note, PCR was positive in five out of five...
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Tài liệu Báo cáo Y học: Purification, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot
Ngày tải lên :
22/02/2014, 04:20
... Sephadex G -25 M 22- hydrazide gel 525 490 20 5 28 .7 6.6 1 020 0 784 ND 1 12 0.017 6608 6419 328 0 29 44 21 .3 0.638 8.18 ND 26 .3 124 2 100 99 50 45 0. 32 13 ND 41 1947 Ó FEBS 20 02 Molecular identification of a ... varied between 0 .2 and 0.64 depending on the pH and the presence of NP-40; (d) Ó FEBS 20 02 Molecular identification of a liver ecto-ATPDase (Eur J Biochem 26 9) 23 79 Fig Expression of the chicken liver ... at 72 °C A PCR product of 1.5 kb that corresponded to the length of the coding region of the chicken ecto-ATPDase was obtained An aliquot (2 lL) of the reaction mixture was used for further...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
Ngày tải lên :
07/03/2014, 15:20
... Asp234, Ile246, Tyr248, Asn255 and Gln256 of the ricin B-chain in the second sugar binding site [28 ] The exceptions are the replacement of Trp38 fi Ser in the ML2p B-chain and Tyr2 52 fi Phe in the ... corresponding to the ricin B-chain Leu117) or Met (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu1 52; and Met234 of the ML1p and ML2p B-chains ... cuts the amplification product of ml2p, giving 66, 121 and 24 3 bp fragments, and the amlpification product of ml3p and ml3.1p, giving 50 and 365 bp fragments The 24 6 bp PstI restriction fragment, 24 3...
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Báo cáo khoa học: Cloning and expression of the first nonmammalian interleukin-11 gene in rainbow trout Oncorhynchus mykiss pdf
Ngày tải lên :
07/03/2014, 16:20
... Mouse Rat 20 8 26 1 82 204 26 178 199 21 178 199 21 178 199 21 178 199 21 178 20 .4 4.9 (9) 15.4 (28 ) 9.9 (18) 7.91 20 .5 1 9.0 (16) 13.5 (24 ) 12. 4 (22 ) 9.83 19.1 0 12. 4 (22 ) 23 .0 (41) 11.8 (21 ) 11.16 ... decidua and fetoplacental development in mice Genes Dev 12, 22 34 22 43 22 Wang T & Secombes CJ (20 03) Complete sequencing and expression of three complement components, C1r, C4 and C1 inhibitor, of the ... 11.8 (21 ) 11.16 19.4 0 11 .2 (20 ) 23 .6 ( 42) 11.8 (21 ) 10.71 19 .2 0 9.0 (16) 21 .9 (39) 11.8 (21 ) 11.16 19 .2 0 9.0 (16) 21 .3 (38) 11.8 (21 ) 11.16 Percentage of amino acid in the mature peptide Number...
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Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc
Ngày tải lên :
17/03/2014, 10:20
... from Hanes plots Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined by ... 3 12 271 29 9 20 6 3045 416 78 9.3 0.10 0.65 3.83 22 .1 6.4 37.5 21 6.7 100 87 96 66 Ó FEBS 20 03 Expression of quinoline 2- oxidoreductase genes (Eur J Biochem 27 0) 1573 Table Metal content, amount of ... indicated in Fig 4B The absence of Mo(V)signals reveals the g2-component of FeSII It is noted that the g-factors of the FeSI and II signals of the Qor proteins from the wild-type strain and from P putida...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
Ngày tải lên :
23/03/2014, 10:20
... S.A.) The arrows denote the size of the L schmitti CHH transcript and the CHH fragment amplified by PCR RNA ribosomal subunits 18S and 28 S are shown FEBS Journal 27 3 (20 06) 5669–5677 ª 20 06 The ... dsRNA They suggest that the complete silencing of the gene transcript could not be achieved due to dilution of dsRNA in the feeding stage of the female tick [31] On the other hand, Acosta and coworkers ... observed the amplification by RT-PCR of a defined band of 26 0 bp in the saline treated group only These results suggest the possible complete degradation of the CHH transcript because of dsRNA...
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Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc
Ngày tải lên :
23/03/2014, 21:20
... performed Northern blot analyses with human and murine RNAs (Fig 2) In human and mouse, specific signals were obtained at a size of approximately 2. 5 and 2. 3 kb, respectively The size of the murine ... indicating the presence of Nicn1 transcripts in all tissues of the developing embryos at the investigated developmental stages 524 4 B Backofen et al (Eur J Biochem 26 9) Ó FEBS 20 02 Fig Quantification of ... The promoters of the canine and murine NICN1 genes lack a TATA box-motif In both species, the sequences in the promoter regions fulfill the criteria of CpG islands (CpG obs/exp > 0.6 and GC > 50%)...
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Báo cáo Y học: Brassica napus soluble epoxide hydrolase (BNSEH1) Cloning and characterization of the recombinant enzyme expressed in Pichia pastoris docx
Ngày tải lên :
31/03/2014, 08:20
... J 9, 22 9 24 1 21 Thelen, J.J & Ohlrogge, J.B (20 02) Metabolic engineering of fatty acid biosynthesis in plants Metab Eng 4, 12 21 22 The Arabidopsis Genome Initiative (20 00) Analysis of the genome ... s, 65 °C for 20 s and 72 °C for The first PCR reaction was diluted 50-fold and lL used in the second PCR The primers used for the nested reaction used the nested universal primer and a second ... Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 22 7, 680–685 26 Bellevik, S., Summerer, S & Meijer, J (20 02) Overexpression of Arabidopsis thaliana soluble...
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Cloning and characterization of the promoter of the cancer associated gene, FAT10
Ngày tải lên :
03/10/2015, 20:57
... OF THE PROMOTER OF FAT10 GENE iii 18 2. 1 Background 19 2. 2 Materials and Methods 20 2. 2.1 2. 2 .2 2 .2. 3 2. 2.4 2. 2.5 2. 2.6 Cell lines and Transfection Reverse Transcript PCR DNA sequencing reaction ... lines 28 2. 4 37 Discussion CHAPTER III EVALUATION OF THE ROLE OF P53 IN THE REGULATION OF FAT10 AT THE TRANSCRIPT LEVEL 32 35 40 3.1 Background 41 3 .2 3 .2. 1 3 .2. 2 3 .2. 3 3 .2. 4 3 .2. 5 Materials and ... al., 20 02; Raasi et al., 1999) Chapter II Isolation and Characterization of the Promoter of the FAT10 Gene 32 2.3 .2 The responsive domain of the FAT10 promoter to TNF-α and IFN-γ resides in the...
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Cloning and expression of the plasmodium falciparum metacaspase gene PfMCA1
Ngày tải lên :
03/10/2015, 20:57
... 23 2. 1. 12 SEG analysis of PfMCA1 25 2. 2 E coli 2. 2.1 Bacterial strains and culture 25 2. 2 .2 Plasmids 26 2. 2.3 Molecular cloning 26 2. 2.4 Preparation of ... .26 2. 2.5 Transformation and screening 26 2. 2.6 DNA sequencing 27 2. 2.7 Induction of protein expression .27 2. 2.8 Isolation of bacterial protein extracts .27 2. 2.9 ... expression .22 2. 1.8 PCR amplification of yeast-optimized PfMCA1 .22 2. 1.9 Site-directed mutagenesis of PfMCA1 22 2. 1.10 Molecular cloning and screening .23 2. 1.11 DNA sequencing...
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Isolation, cloning and characterization of the FSH beta gene promoter of the chinook salmon (oncorhynchus tshawytscha)
Ngày tải lên :
08/11/2015, 17:15
... INTRON1F -2 #20 INTRON1F-3 #21 INTRON1F-4 #22 GSP2F* #23 GSP4F* #24 INTRON2F-1 #25 INTRON2F -2 #26 GSP5F* #27 GSP6F* #28 TSS 324 * #29 INTRON2R-1 #30 INTRON2R -2 #31 STAR6* # 32 AUG2* #33 AUG600 #34 SEP2* ... plasmid preparation was air-dried and the DNA was resuspended in 300 µL deionized water 32 2.3.7 DNA sequencing of the 2. 2 kb PCR fragment The 2. 2 kb PCR fragment was then sequenced using ABI PRISMTM ... 95 65 72 72 Time 30 s 30 s 30 s 10 Hold Table 10: Cycling parameters of PCR cycle 2. 4 .2 Cloning of the 1kb intronic enhancer into the vectors Both the amplified kb fragment and the CAT6 and EGFP...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
Ngày tải lên :
14/02/2014, 19:20
... CD2-1 CD2 -2 CD2-3 CD2 1099 Inter- 600 25 0 767 150 934 IB: -v-KIND E F CD2-1 CD2-1-1 634 CD2-1 -2 667 CD2-1-3 701 CD2-1-4 CD2-1-5 735 CD2-1-6 7 02 CD2-1-7 668 CD2-1-8 635 CD2-1-6-1 7 02 CD2-1-6 -2 ... v-KIND KIND2 KIND2-1 KIND2 -2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2 -2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 v KIND v-KIND KIND2 KIND2-1 + ... CD2-1-6-3 Interaction PD Input GST CD2-1-1 CD2-1 -2 CD2-1-3 CD2-1-4 CD2-1 767 25 0 150 PD 734 Input GST CD2-1-5 CD2-1-6 CD2-1-7 CD2-1-8 CD2-1 600 734 744 755 25 0 PD Input GST CD2-1-6-1 CD2-1-6-2...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
Ngày tải lên :
19/02/2014, 12:20
... (Met1)-ONC (M23L) by the addition of AAP, as described in the Materials and methods Completion of the hydrolysis was confirmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass ... 8711–8718 21 Beaumelle, B., Taupiac, M.P., Lord, J.M & Roberts, L.M (1997) Ricin A chain can transport unfolded dihydrofolate reductase into the cytosol J Biol Chem 27 2, 22 097 22 1 02 22 Leland, P.A., ... other methods for the production and purification of the enzyme [13] The molecular mass of each purified product, as measured by MALDI-TOF MS, was 11 947.87 2 for (Met1)-ONC (M23L) and 11 838 .21 ±2...
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Báo cáo Y học: Novel fish hypothalamic neuropeptide Cloning of a cDNA encoding the precursor polypeptide and identification and localization of the mature peptide pptx
Ngày tải lên :
08/03/2014, 16:20
... in the goldfish brain The localization of its transcript in the goldfish brain was further investigated 45 °C and 2. 5 at 72 °C, and of 30 cycles for 30 s at 94 °C, 30 s at 50 °C and 2. 5 at 72 °C ... Existence of galanin in lumbosacral 23 24 25 26 27 28 29 sympathetic ganglionic neurons that project to the quail uterine oviduct Endocrinology 141, 44 02 44 12 Ukena, K & Tsutsui, K (20 01) Distribution ... detect the signal in the NT, which may be due to the low expression of goldfish LPXRFamide peptide mRNA Otherwise, the localization of immunoreactive cell bodies in the NT may suggest the presence of...
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Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf
Ngày tải lên :
08/03/2014, 23:20
... 10 10 11 11 12 12 13 13 14 14 15 15 16 16 17 17 18 18 19 19 20 20 21 21 22 22 23 23 24 24 16000b >20 08 22 3 334 1669 >25 79c 761 >21 47c 945 1 629 11 02 1063 1437 >1759c 1680 121 3 20 2 145 681 801 ... Mouse Human Size (bp) 1 2 3 4 5 6 7 8 9 10 10 11 11 12 12 13 13 14 14 15 15 16 16 17 17 18 18 19 19 20 20 21 21 22 22 23 23 24 24 25 25 >178a >110a 9500b >15 432c 129 153 134 134 165 165 156 ... 114/ 126 d 120 120 21 2 20 9 85 91 168 168 1 02 1 02 234 23 4 138 138 1 82 1 82 160 160 174 180 74 74 71 65 23 6 22 4 163 163 >411a > 323 a 5¢ end 3¢ end No Size (bp) Class ACCTGgtaagacaag CCCAGgtaagtgatg 1 2...
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A Compilation of the Messages and Papers of the Presidents Section 2 pot
Ngày tải lên :
17/03/2014, 02:20
... for the accommodation of Congress and of the President and of the public offices of the Government have made a report of the state of the buildings designed for those purposes in the city of Washington, ... receive the posts and to run the line of partition between our territory and that of the King of Spain A Compilation of the Messages and Papers of the Presidents 22 Attempts to alienate the affections ... Congress a report of the Secretary of State, with a copy of an act of the legislature of the State of Kentucky consenting to the ratification of the amendment of the Constitution of the United States...
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Compilation of the Messages and Papers of the Presidents Section 2 pptx
Ngày tải lên :
17/03/2014, 02:20
... township, and saving and excepting the lands ceded under the act of September 1, 1888 (25 Stat., 4 52) , for the purposes of a townsite, will on the 17th day of July, 19 02, at and after the hour of 12 ... miles of the boundary line of the town of Pocatello, Idaho and saving and excepting the lands ceded under the act of September 1, 1888 (25 Stat, 4 52) , for the purposes of a townsite, will on the ... sons of the North and the sons of the South in the times that tried men's souls The men who in the last three years have done so well in the East and the West Indies and on the mainland of Asia...
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Báo cáo khoa học: Bovine tryptases cDNA cloning, tissue specific expression and characterization of the lung isoform ppt
Ngày tải lên :
17/03/2014, 09:20
... for BLCT [11, 32] MH+ theoretical Peptide 26 93.1 23 24.7 1947.3 1903.1 1576.0 1490.7 14 42. 5 1366 .2 1344.5 1330.8 127 2.4 126 3.7 121 7.5 1174.1 10 72. 2 1071 .2 1064.3 909.1 680.4 26 93.7 23 24.1 1947.9 ... 14 42. 1 1365.7 1344.4 1330.4 127 1.9 126 3.1 121 7.0 1174.5 10 72. 0 1071.0 1064.5 909 .2 680.9 1 62 187 41–61 20 2 22 0 62 76 23 0 24 5 113– 126 167–180 88–100 188 20 1 150–161 77–87 88–99 139–149 150–160 127 –137 ... Clin Invest 99, 26 91 27 00 19 Steinhoff, M., Vergnolle, N., Young, S.H., Tognetto, M., Amadesi, S., Ennes, H.S., Trevisani, M., Hollenberg, M.D., Ó FEBS 20 03 20 21 22 23 24 25 26 27 28 29 30 Tissue-specific...
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Báo cáo Y học: Trehalose-phosphate synthase of Mycobacterium tuberculosis Cloning, expression and properties of the recombinant enzyme pdf
Ngày tải lên :
17/03/2014, 10:20
... emerged in the region suggesting a molecular weight of 22 0 kDa (Fig 3) These data suggest that the active TPS probably exists as a tetramer Properties of the recombinant TPS The activity of the purified ... on the activity of the TPS was determined as shown in Figs and Fig shows the effect of increasing concentrations of either UDP– glucose or GDP–glucose in the presence of saturating levels of ... development of hyphae and did decrease the infectivity of the organism, but it was not lethal Thus, the rate of growth of the mutant at 30 °C was indistinguishable from the growth rate of the wild-type,...
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The History and Antiquities of the Doric Race, Vol. 2 of 2 pdf
Ngày tải lên :
17/03/2014, 23:20
... Persian, after the expulsion of the tyrants, restored it in their cities as the desired form of government. (22 ) In Athens Cleisthenes had deprived the union of the houses (the last support of the aristocracy) ... among any other people to attain the same end, and had their basis in the same notions On the other hand, it was the pride of the Spartans, that their laws had proceeded from the oracle of the Pythian ... derived from the possession of land, also carried on the extensive commerce of these two states; otherwise the wealth of the merchant would soon have exceeded that of the landowner In the Doric...
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