amplification method for the detection of vibrio nigripulchritudo in shrimp
Fisheries science JSFS , tập 76, số 2, 2010 3
Ngày tải lên :
19/07/2016, 19:43
... considered to take place in the intestine following active absorption of Na? and Cl- [3, 5] In the posterior part of the intestine, including the rectum, osmolality of ingested seawater is reduced ... is the proportion of individuals belonging to the ith item in the fish gut contents, bi is the selectivity index for the ith item, Ai is the number of available prey individuals belonging to the ... although the amount of this substance was smaller in the latter These findings suggest that the mechanisms of calcification induced in the CaDPD scales in vitro and the control scales in vivo...
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BÁO CÁO " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu s vannamei) " pptx
Ngày tải lên :
02/04/2014, 10:20
... Cần thơ: 80-90 Elovaara A.K 2003 Shrimp Farming Manual Practical technology for Intensive shrimp production ISBN: 0-9708605-1-X Printed in the United States of America Ferraris R.P., F.D Parado-Estepa, ... and J.M Ladja 1987 Osmotic and chloride regulation in the hemolymph of the tiger prawn Penaeus monodon during molting in various salinities Marine Biology 95:377-385 Funge-Smith S.J., A.C Taylor, ... permeabilities of gill lamina cuticle of the crayfish Astacus leptodactylus (E.) Journal of Physiology 363:377-401 Cameron J.N and P Thomas 1992 Calcitonin-like immunoreactivity in the blue crab:...
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Báo cáo nghiên cứu khoa học: " ẢNH HƯỞNG CỦA ĐỘ MẶN THẤP LÊN ĐIỀU HÒA ÁP SUẤT THẨM THẤU + + VÀ HOẠT TÍNH MEN NA /K ATPASE Ở TÔM THẺ CHÂN TRẮNG (Litopenaeu svannamei)" ppsx
Ngày tải lên :
22/07/2014, 11:21
... Cần thơ: 80-90 Elovaara A.K 2003 Shrimp Farming Manual Practical technology for Intensive shrimp production ISBN: 0-9708605-1-X Printed in the United States of America Ferraris R.P., F.D Parado-Estepa, ... and J.M Ladja 1987 Osmotic and chloride regulation in the hemolymph of the tiger prawn Penaeus monodon during molting in various salinities Marine Biology 95:377-385 Funge-Smith S.J., A.C Taylor, ... permeabilities of gill lamina cuticle of the crayfish Astacus leptodactylus (E.) Journal of Physiology 363:377-401 Cameron J.N and P Thomas 1992 Calcitonin-like immunoreactivity in the blue crab:...
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Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot
Ngày tải lên :
31/03/2014, 21:21
... U of the reporter DNA polymerase (the arrow in Fig 1) During continued addition of the polymerase, the enzyme activity increased in parallel with the activity of the control experiment in the ... explain the end of the activity period of DNA polymerases Instead of assuming a neutralization of the inhibitory activity, the DNA polymerases could bind effectors, which induce the release of ... inhibited by PMLA contained in the extracts The inhibitory activity during the cell cycle The inhibitory activity during the cell cycle was measured in the presence of 0.4 U of (added) purified DNA...
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effects of magnesiumphosphoruspotassium containing in water on molting, growth and survival rate of the white shrimp (litopenaeus vannamei) juveniles, reared in low salinity water
Ngày tải lên :
22/09/2015, 12:38
... observation of feeding activity of shrimp Salinity water was 2‰ Determination of molting intervals of shrimp: Molting of shrimp were recorded daily at 7~8:00 AM through the old shell in the net case Shrimp ... a Mean of molting interval Molting interval Fig.5: Molting intervals of white shrimp (L.vannamei) 4.2.2.2 Weight gain of white shrimp after molting After first molting, WG of shrimp in the 20-10-10 ... remains until the next molting cycle Duration of the molting cycle depends on the species and age of the shrimp Molting frequency on white shrimp decreased along with increasing the size of shrimp...
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Tài liệu Báo cáo khoa học: The phosphatase activity of the isolated H4-H5 loop of Na+/K+ ATPase resides outside its ATP binding site docx
Ngày tải lên :
19/02/2014, 16:20
... at the rear surface of the N-domain (overview) (A) Docking of pNPP to the ATP binding site of the model containing N- and P-domains [20] The amino acids interacting with the adenine ring of ATP ... MgATP in the dimer formation of a H4-H5 loop protein of Na+/K+ ATPase [55] In conclusion, neither method revealed any indication of a conformational change of the N-domain upon ligand binding in the ... ATP binding to the N-domain This process may eventually induce its bending towards the P-domain and thereby explain the amino acid labeling of the P-domain found with 8-N3-TNP-ADP [25] The same...
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Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt
Ngày tải lên :
17/03/2014, 03:20
... taken for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose (50 mM) to determine ... aggregation of lens proteins and enzymes These protective capabilities have been demonstrated with other, in vitro systems, including prevention of aggregation of insulin B chain following reduction of ... was incorporated inside the ghost cells Ghosts resealed in the presence of acrystallin showed the extra two a-crystallin subunit bands at around 20 kDa confirming the efficient sealing of the protein...
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Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
Ngày tải lên :
24/03/2014, 00:21
... methodology in discriminating conformational changes Effects of pressure on intrinsic ¯uorescence The emission maximum (kmax) and intensity of intrinsic ¯uorescence of Na+/K+-ATPase under increasing ... dissociation, conformational change of protein, and/or the destruction of integral structure (lipid bilayer and protein) The issue of clarifying the precise nature of the interrelationship between these ... wavelength for FITC (470 nm) Pressure-induced changes in BF/F are shown in Fig The pressure dependence of BF/F for the Na+/K+ATPase in the presence of Na+ or K+ were in parallel, resulting in a concave...
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Na+, k+ ATPase in single skeletal muscle fibres and the effects of ageing, training and inactivity
Ngày tải lên :
04/12/2015, 13:59
... of these isoforms include global knockout models, which are not successful in helping determine functions of each isoform, as a knockout of either of the three α isoforms leads to the death of ... However, the effects of a longitudinal training program on NKA isoform abundance in human are unknown Therefore Chapter investigated whether 12 weeks of high-intensity interval training (HIT) in aged ... muscle has been effective in detecting general adaptability of the [3H]ouabain binding site content and NKA isoforms following training, inactivity induced by injury and ageing (Leivseth & Reikeras,...
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Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx
Ngày tải lên :
07/03/2014, 16:20
... depress the increase in the ouabain-insensitive activity due to increasing the KCl concentration On the other hand, increasing the Na+ concentration had no effect on the ouabain-insensitivity of pNPPase ... oligomycin In the absence of oligomycin, increasing the NaCl concentration decreased pNPPase activity (Fig 2A) In the presence of oligomycin, increasing the NaCl concentration obviously increased the ... This antibiotic binds in the N-terminal domain of the a subunit [18], whereas ATP binds in the domain containing Lys501 of the a subunit [27] NaE1–oligomycin is an arrested form, whereas NaE1ATP...
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Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
Ngày tải lên :
16/03/2014, 13:20
... phosphorylation of MCM4, together with the presence of geminin, which inhibits the ability of CDT1 to load MCM proteins onto chromatin, may help prevent re-replication of DNA in the G2 and M phase During interphase, ... prevent binding of MCM complex to chromatin [12] In contrast, a recent finding suggests that an intermediate level of phosphorylation of MCM4 is required for chromatin binding during interphase ... cell cycle in a site-specific manner Cyclin-dependent protein kinase is involved in the phosphorylation of MCM4 To determine which kinase is involved in the phosphorylation of MCM4 in phases G2...
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Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
Ngày tải lên :
23/03/2014, 06:20
... are introduced, at least in some of the promoters Furthermore, although we observed distinct gaps for MLL1 (as well as its interacting proteins) in immunofluorescence staining experiments in the ... Notably, in our studies, we also found the presence of these distinct gaps for MLL1 and interacting proteins in mitotic cells in a population of asynchronous cells (data not shown) These results indicate ... were localized in the less intense DAPI-stained regions in the nucleus (representing less condensed euchromatin), leaving gaps in the more intense DAPI-stained regions (representing more condensed...
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Báo cáo khoa học: No evidence for a role in signal-transduction of Na+/K+-ATPase interaction with putative endogenous ouabain potx
Ngày tải lên :
23/03/2014, 17:21
... ouabain or OLF Ouabain-sensitive and -insensitive a-isoforms of Na+/K+-ATPase in rats All isoforms of the catalytic a-peptide of Na+/K+-ATPase have similar, high affinity for ouabain [17] with Kd in ... were interpreted as independent of a small increase in [Na+]i and also occasionally independent of the subsequent increase in [Ca2+]i [10], whereas in other publications, activation of the Ras/MAPK ... mixture of ouabain-insensitive and ouabain-sensitive isoforms, of which the latter may have a sublocalization critical for the observations looking at ouabain as a signal-transducer Experiments pointing...
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Báo cáo Y học: Na+/K+-ATPase as a signal transducer Zijian Xie and Amir Askari docx
Ngày tải lên :
24/03/2014, 00:21
... energy transducing ion pump whose partial inhibition by ouabain initiates the increase in [Ca2+]i, and the other the signal transducing pool of the enzyme which, through protein–protein interactions, ... Ouabain interaction with /Ca the other pool alters protein–protein interactions to activate the indicated signaling pathways The events placed in the grey box have been shown to be independent of ... Significantly, in vascular smooth muscle cells, the proximal events of ouabain-induced signaling also involve the activation of Src and the transactivation of the EGF receptor [35] Ouabain signaling distinct...
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Báo cáo y học: " Distinguishing between linear and exponential cell growth during the division cycle: Single-cell studies, cell-culture studies, and the object of cell-cycle research" ppsx
Ngày tải lên :
13/08/2014, 23:20
... family gatherings When measuring time occurs the grandchildren stand against the board, a line is drawn at the top of their head, and the date of the line is noted The board on which some of these ... order as required by the method Further, the labeling is performed prior to any binding to the membrane, so there is no perturbation of the cells The use of the membrane-elution method has been discussed ... sudden increases in the pattern of biosynthesis of the cell Thus, a cell that grows adding 0.1 unit of mass at each time interval would this continuously for one cell cycle At the instant of division...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
Ngày tải lên :
16/02/2014, 09:20
... Discussion In the present work, the cytotoxic properties of the minor structural proteins (VP2 and VP3) of the MPyV were studied in the absence of other virus components as well as during the late ... late phase of virus infection The role of the minor structural proteins in the replication cycle still remains obscure Our previous analysis of MPyV mutated in the minor structural proteins VP2 ... apoptosis induced during MPyV infection To test whether the minor proteins function as inducers of apoptosis also during infection, we prepared the MPyV genome mutated in ATG codons of both VP2...
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Tài liệu Báo cáo khoa học: Bioimaging of the unbalanced expression of microRNA9 and microRNA9* during the neuronal differentiation of P19 cells doc
Ngày tải lên :
18/02/2014, 17:20
... strands of the piRNA hairpin are involved in formation of the piRNA-interacting complex (piRC) and function in the transcriptional gene silencing of retrotransposons and genetic elements in germline ... Control Control for for for for for for for for expression of pri-miR9-1 expression of pri-miR9-1 expression of pri-miR9-2 expression of pri-miR9-2 expression of pri-miR9-3 expression of pri-miR9-3 ... for in vivo imaging of gene expression of endogenous pri-miR9 during neurogenesis To image in vivo the endogenous expression of the pri-miR9 in small animals, 2.5 · 106 of P19 cells bearing the...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
Ngày tải lên :
21/02/2014, 00:20
... Fig The in uence of Mg2+ on the kinetic properties of APase from E coli The in uence of Mg2+ on the kinetic properties of APase from E coli in the presence of a Zn2+ to dimer ratio of : in 2A2M1P ... reduces the enzymatic activity indicating that binding of the last Zn2+ ion, probably to the second M3 site, cannot supplement the role of magnesium in the kinetic mechanism In the presence of Mg2+, ... for Mg2+ also Mg2+ binds to the low affinity subunit enhancing the conformational change in path C, and enabling a conformational change in path A, thereby increasing the rate of both cycles In...
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Tài liệu Báo cáo khoa học: Unfolding and aggregation during the thermal denaturation of streptokinase pptx
Ngày tải lên :
21/02/2014, 03:20
... number of two-state transitions In these fittings the heat capacity change, DCp, for the unfolding of each domain was fixed by using the values obtained from the analysis of the Cp curves of the corresponding ... using the spectra obtained for solutions in the absence of dye or protein RESULTS Thermal unfolding of SK under equilibrium The thermal denaturation of intact SK and a set of SK fragments including ... to the thermograms, which may bias the resulting number of unfolding transitions We have shown here that the use of changes in the heat capacity of unfolding determined independently for the...
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Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
Ngày tải lên :
22/02/2014, 04:20
... acid cycle, and the glyoxylate cycle by examining the individual phylogenies of the 21 subunits comprising the 14 enzymes of these pathways as they occur in eukaryotes, speci®cally in higher plants ... result in proper targeting of the protein to the mitochondrion may underly the origin of these highly diverse compartment-speci®c isoforms ACKNOWLEDGEMENTS We thank Marianne Limpert for help in preparing ... our aim was not to perform an exhaustive analysis but to obtain an overview of the patterns of similarity for the enzymes of these pathways in plants and the relationships of their differentially...
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