amniotic and placental mesenchymal stem cell isolation and culture

Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction" ppt

Ngày tải lên : 18/06/2014, 19:20

... marrow-derived mesenchymal stem cells (BMDMSCs), hematopoietic stem/ progenitor cells, and cells of embryonic origins, the ethical issue regarding the source and safety of allo- and xeno-grafting ... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3) Note ... groups and 3, and significantly lower in group than in group at 24 h and 72 h after IR injury On the other hand, this protein expression was markedly lower in group than in groups and 3, and notably...

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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Ngày tải lên : 10/08/2014, 05:21

... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues using ... primary cell cultures of hMSCs with HPV 16 E6E7 and developed the KP cells [6], which were then overexpressed with hTERT Several single -cell derived clones were isolated and 3A6, 1C5 and 3G11...

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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)

Ngày tải lên : 09/09/2015, 11:21

... endothelial cells and parenchymal cells after APAP overdose before cell injury [29] Both hydrogen peroxide and peroxynitrite decomposition products, OH and NO2 radicals can initiate LPO and lipid and ... Lai, W Wong, Y Y Dan, S K Lim and H K Ho, Mesenchymal stem cell- derived exosomes promote hepatic regeneration in drug-induced liver injury models, Stem Cell Research and Therapy, 5(3):76 (2014) ... and culture conditions 47 2.3.2 In vitro cytotoxicity test of exosomes and Vitamin E analogs (-TP and T3 isomers) .48 2.3.3 In vitro cellular uptake of Vitamin E analogs (-TP and...

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NGHIÊN CỨU SỬ DỤNG TẾ BÀO GỐC TRUNG MÔ (MESENCHYMAL STEM CELL - MSC) TRONG ĐIỀU TRỊ UNG THƯ ĐẠI - TRỰC TRÀNG TRÊN CHUỘT (SPRAGUE DAWLEY) ppt

Ngày tải lên : 20/03/2014, 09:21

... Therapeutic potential of mesenchymal stem cells producing interferonalpha in a mouse melanoma lung metastasis model, Stem Cells 26, (2008), 2332-8 Satija, N K et al., Mesenchymal stem cell- based therapy: ... cell- based therapy: a new paradigm in regenerative medicine, J Cell Mol Med 13, 2009 STEM CELL RESEARCH USING MESENCHYMAL (MESENCHYMAL STEM CELL - MSC) IN THE TREATMENT OF CANCER AGENCY - RECTUM RATS ... that the mesenchymal stem cells injected into mice at the dose of 107 cells/ml can inhibit the proliferation of cancer cells The number at tumors in mice injected with mysenchymal stem cells at...

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Báo cáo y học: "Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cell" ppsx

Ngày tải lên : 10/08/2014, 10:20

... Yarmush ML: Mesenchymal stem cells: mechanisms of immunomodulation and homing Cell Transplant 2010, 19:667-679 Lee PH, Kim JW, Bang OY, Ahn YH, Joo IS, Huh K: Autologous mesenchymal stem cell therapy ... human mesenchymal stem cells generate osteosarcoma-like lesions in the lung Stem Cells 2007, 25:1586-1594 doi:10.1186/1423-0127-18-54 Cite this article as: Chang et al.: Mesenchymal stem cell ... Umbilical Cord Blood-Derived Mesenchymal Stem Cell Transplantation in Experimental Lupus Nephritis Cell Transplant 2011, 20:245-257 32 Parekkadan B, Milwid JM: Mesenchymal stem cells as therapeutics...

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báo cáo khoa học: "The research on the immuno-modulatory defect of Mesenchymal Stem Cell from Chronic Myeloid Leukemia patients" pdf

Ngày tải lên : 10/08/2014, 10:21

... Besides HSCs, there also existed another kind of stem cells called MSCs (Mesenchymal Stem Cells), they could differentiated into stroma cells and acted as the “niche” in the micro-environment[24] ... Army Cell preparations and culture Isolation and culture of bone marrow-derived CML hemangioblasts were performed as described previously with some modifications[19,21] Briefly, mononuclear cells ... by Annexin-V and PI double staining and analyzed by flow cytometry Apoptosis of T cells was analyzed in T cells alone (Ts), normalMSC cocultured with activated T cells (MSC + Ts), and CML patient-derived...

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Báo cáo y học: " Defining human mesenchymal stem cell efficacy in vivo" ppsx

Ngày tải lên : 11/08/2014, 03:20

... pericytes? Cell Stem Cell 2008, 3:229-230 da Silva ML, Caplan AI, Nardi NB: In search of the in vivo identity of mesenchymal stem cells Stem Cells 2008, 26:2287-2299 Caplan AI, Bruder SP: Mesenchymal stem ... perivascular origin for mesenchymal stem cells in multiple human organs Cell Stem Cell 2008, 3:301-313 Delorme B, Chateauvieux S, Charbord P: The concept of mesenchymal stem cells Regen Med 2006, ... HM: Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture- expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy...

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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx

Ngày tải lên : 13/08/2014, 01:20

... multipotent mesenchymal stem cells from the bone marrow of rhesus macaques Stem Cells Dev 2005, 14:440-451 doi:10.1186/1742-4690-8-3 Cite this article as: Nazari-Shafti et al.: Mesenchymal stem cell ... responsible to cell cloning and cultures EF, TN, and CS conducted differentiations UR and CB were responsible for HIV-1 infection studies AC and CD were responsible for tissue collections RI, DM, and EA ... and their 95% confidence intervals were calculated R-computing software was used to plot the graphs Abbreviations MSCs: mesenchymal stem cells; ASCs: adipose tissue derived mesenchymal stem cells;...

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Dynamic mechanical stimulation for mesenchymal stem cell chondrogenesis in an elastomeric scaffold

Ngày tải lên : 09/09/2015, 08:12

... Microscopy (SEM)…… 40 3.3 Cell culture and Chondrogenic Differentiation……………………………….…40 3.3.1 MSC Isolation and Culture ……………………………………….…….40 3.3.2 Primary Chondrocyte Isolation and Culture ……………………….… ... chondro-progenitor mesenchymal cells into precartilage condensations (Fig 5) Cellular condensation is dependent upon signals initiated by cell cell and cell matrix interactions and is associated ... avoid hypertrophy and cell dedifferentiation, but donor site morbidity and fibrocartilage formation are still inevitable drawbacks Thus, mesenchymal stem cell (MSC) as an alternative cell source is...

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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 10

Ngày tải lên : 10/09/2015, 09:01

... the stem cells and/ or attract the endogenous or exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... MD Fibronectin, vitronectin, and collagen I induce chemotaxis and haptotaxis of human and rabbit mesenchymal stem cells in a standardized transmembrane assay Stem Cells Dev 2007 Jun;16(3):489-502 ... clinical trial stage, and showed promising results till now (281) Previous studies showed that BM MSCs and synovium derived mesenchymal stem cells (SMSCs) are promising stem cell sources for cartilage...

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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1

Ngày tải lên : 10/09/2015, 09:10

... Hering) Oval cells that subsequently generate hepatocytes and ductular cells Pancreatic stem cells Intraislet, nestin-positive cells, oval cells, duct cells Beta cells Skletal-muscle stem cells or ... during harvest, stem cells are classified into two groups of embryonic stem cells (ESC) and adult stem cells (70) (figure 1.7 adopted from (71)) Figure 1-7 Stem cell classification and differentiation ... basal and mucussecreting cells, bronchiolar Clara cells, alveolar type II pneumocyte Mucous and ciliated cells, type I and II pneumocytes Stem cells of the intestinal epithelium Epithelial cells...

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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 2

Ngày tải lên : 10/09/2015, 09:10

... Figure 3-3 Flow cytometry analysis of the stem cells surface markers Harvested cells were positive for CD29, CD44, and CD90, and negative for CD14, CD31, CD45, and CD34 59 3.4.2 Prussian blue ... concentration in the culture media would increase the intracellular iron content of MSCs 61 Average iron content per cell (pg /cell) 200 150 100 50 Average iron content per cell (pg /cell) 0µg/ ml 25µg/ ... closed and rested for one week MSCs (107 cells) labeled with 50µg/ml ferucarbotran (10pg /cell) were seeded in ml hyaluronan and used for intra-articular injection of the experimental knee and ml...

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