amino acid sequences of caseins

... sequence of the open reading frame (ORF) of their cDNAs and the corresponding aa sequences, in Table Summary of the purification of DNases I from the pancreases of three species of snake The results of ... I The use of two primers based on aa sequences Ó FEBS 2003 A mechanism from cold-blooded to warm-blooded vertebrates (Eur J Biochem 270) 311 Fig Alignment of the amino acid sequences of snake ... Comparison of the three primary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas Derivation of the amino acid sequence of ovine DNase and revision of the previously...

Ngày tải lên: 20/02/2014, 23:20

... network involves the side chains of the amino acids in positions 53 and 56 in a helix B, 186 in b strand 3A, and position 334 in b strand 5A (Fig 1; the numbering of amino acids in PAI-1 is according ... combination of amino acids in these positions resulted in an accelerated latency transition Substitution of the Lys residue in position 335 counteracted the accelerating effect of some of the substitutions ... the shutter region affect the rate of latency transition by affecting the rate of passage of the RCL through the gate region Based on the aminoacid sequence of the RCL and b strand 5A being directly...

Ngày tải lên: 22/02/2014, 07:20

... alignment of PSY1 and PSY2 protein derived from analysis with The predicted protein sequences were 412 and 438 amino acids for PSY1 and PSY2, respectively Alignment of the two protein sequences ... sequence contained residues to 91–95 of PSY2 protein, the software always detected a chloroplastic transit peptide of 86 amino acids This may be due to the presence of specific motifs beyond the first ... characterization of the tomato Psy1 and Psy2 genes and also provide the deduced complete amino acid sequences of the two enzymes, together with new insights into the transcriptional regulation of Psy1...

Ngày tải lên: 07/03/2014, 05:20

... denote the amino acid sequences derived from HIV-1 and SIVmac239, respectively The ratio of the amount of p24 to that of p55 of each virus is shown at the bottom A representative of three independent ... insight into the effects of the G-to-E single amino acid substitution at the 116th position of the CA on its three-dimensional (3-D) structure, the 3-D model of the N-terminus of the CA was constructed ... aspartic acid) A single amino acid substitution in CA rescued impaired replicative capability in human cells To determine whether the single amino acid substitution at the 116th position of the...

Ngày tải lên: 13/08/2014, 01:20

... indispensable amino acids that the fish cannot synthesize and (ii) a supply of either dispensable amino acids or sufficient amino nitrogen to enable fish to synthesize them The concept of balance amino acids ... 2013) Lacking of information on the amino acids requirement of striped catfish is therefore the main constrain on utilization of supplemental amino acids and thereby development of more sustainable ... reduced either lacking or excessing any amino acids 3.5 ADC of EAA of ingredients The ADC of essential amino acids in ingredient were shown in Table Table ADC (%) of EAA (FM-fishmeal, SB-soybean, WB-wheat...

Ngày tải lên: 17/10/2015, 08:30

... Association of Feed Control Officials, a metal amino acid chelate is lithe product resulting from the reaction of a metal ion from a soluble salt with amino acids with a mole ratio of one mole of metal ... 47 76 xx Contents EVALUATION OF THE NUTRITIONAL EFFICIENCY OF AMINO ACID CHELATES 86 Silvano Maletto and Germano Cagliero AN ASSESSMENT OF LONG TERM FEEDING OF AMINO ACID CHELATES 106 Robert B ... of a specific mineral in the feed; (b) The Roles of Amino Acid Chelates in Animal Nutrition an imbalance of another mineral or nutrient including certain vitamins, amino acids and fats, any of...

Ngày tải lên: 15/02/2014, 16:20

... [14C]-labelled amino acids or [32P]-labelled tRNA Assay method Aminoacylation of transcript tRNA with [14C]-labelled amino acid Aminoacylation of [32P]-labelled transcript tRNA Arg Arg Cav Cav Source of ... comparison of amino acid utilization by the plant arginyl-tRNA synthetases The aminoacylation level attained in the presence of L-arginine was compared to that in the presence of mM of the analogue ... be aminoacylated with arginine to a level of approximately 0.05 pmol amino acid ⁄ pmol tRNA The corresponding soybean transcript had an arginine acceptance level of approximately 0.1 pmol amino...

Ngày tải lên: 18/02/2014, 13:20

... Materials and methods As a first step, all available sequences of hcHATs and hcHAT-like proteins were collected (Table 1) using the amino acid sequences of human 4F2hc (GenBank accession number M21904) ... above-mentioned hcHAT sequences from nematodes, these proteins from Caenorhabditis elegans [34] have been named as amino acid transporter glycoproteins (ATG) Of the two groups, ATG1 Origin of rBAT and ... possible evolutionary events leading to separation of the heavy-chain subunit of these amino acid transporters from the enzymes involved in the metabolism of starch and related saccharides Results and...

Ngày tải lên: 18/02/2014, 13:20

... proline, aspartic acid, serine or alanine Simple and double FNR mutants of amino acids C266 and L268 were obtained and characterized It was observed that alteration of the amino acid volume decreases ... efficiencies of wild-type and mutant FNRs plotted as a function of different amino acid physicochemical properties The catalytic efficiencies of wild-type and mutant FNRs from Table (percentage of the ... nicotinamide of NADP+ is precisely tuned by selecting amino acids that face the other side of the tyrosine phenol ring The specific volumes of the above-mentioned residues condition the arrangement of Y308...

Ngày tải lên: 18/02/2014, 17:20

... of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues It is quite rare for such a variety of ... structure of conomarphin was similar to those of other conotoxin cDNAs The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 residues, a proregion of ... physiological role of specific Pro hydroxylation in conotoxins is still elusive 1982 The epimerized d-Phe13 is another striking feature of conomarphin The l -amino acid to d -amino acid epimerization...

Ngày tải lên: 18/02/2014, 17:20

... for individual amino acid contents are of the same order of magnitude as those of the total amino acid contents for glutamine (C), glutamic acid (D), asparagine (E), aspartic acid (F), alanine ... 21.90 1.93 (dark) The standard errors for the individual amino acid contents are of the same order of magnitude as those of the total amino acid contents Phloem exudates and xylem sap were collected ... and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were found to be the main nitrogen compounds in the phloem exudates, amounting to 51% of the total amino acids...

Ngày tải lên: 18/02/2014, 18:20

... the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, the amino acid cysteine (Fig 3) However, it cannot ... conditional lethality of the dfp-707 mutant is that one amino acid residue of the N-terminal PPC decarboxylase (CoaC) domain of Dfp is exchanged [4] For further characterization of the dfp-1 mutation, ... 4¢-phosphopantothenic acid, CTP and cysteine The synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosphopantothenate)...

Ngày tải lên: 19/02/2014, 12:20

... by PCR with primers designed on the basis of sequences of PP3591 from P putida KT2440 The nucleotide sequence of the cloned gene (1026 bp; 341 amino acids; molecular mass, 35 972.8 Da) matched ... respectively) with those of the PP3591 gene of P putida KT2440 Results and Discussion Purication and subunit structure of NMAADH Identication of a gene encoding N-methyl-Lamino acid dehydrogenase (NMAADH) ... carrying pDPKA with a yield of 23% The N-terminal aminoacid sequence of the puried enzyme was identical with FEBS Journal 272 (2005) 11171123 ê 2005 FEBS N-Methyl-L -amino acid dehydrogenase from...

Ngày tải lên: 19/02/2014, 16:20

... and the role of key acidic amino acid residues in staphylococcal nuclease unfolding FEBS Journal 272, 3967–3974 Chen HM, Markin VS & Tsong TY (1992) pH induced folding ⁄ unfolding of staphylococcal ... the sum of fractional multiples of the reference spectra for each structural type Figure 1A shows the CD spectra of W140A, E142O and K133A There are no significant differences from that of the ... the secondary structure of the protein disintegrated Using the peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests a decrease...

Ngày tải lên: 20/02/2014, 01:20

... Tyr Table Amino- acid content of the total proteins of selected mesophiles and hyperthermophiles Calculations were performed using all the open reading frames described in the genomic sequences ... One unit of isomerase activity is defined as the amount of enzyme that produces mmol of product per under the assay conditions Activation of apo-BLXI by metals To examine the effect of bivalent ... the presence of Co2þ or Mn2þ are interesting With a similar inactivation mechanism, Ea of inactivation of T thermosulfurigenes XI is only 490 kJ·mol21 in the presence of Table Effect of metals on...

Ngày tải lên: 22/02/2014, 07:20

... concentration of amino acids In addition, yeast cells possess an amino acid sensing system, localized at the plasma membrane, that transduces information regarding the presence of extracellular amino acids ... whereby a subset of genes is coordinately induced by starvation of the cell for one single amino acid [11] In mammals, our knowledge of the regulation of gene expression by amino acid availability ... ratio of the distribution of the amino acid- regulated genes* versus the distribution of the genes spotted onto the microarray** *Percentage of the representation of one GO term among all the amino...

Ngày tải lên: 07/03/2014, 03:20

... features of the dissociated cofactor were similar to those of FAD The shifting of absorption maxima of the enzyme-bound avin was due to its microenvironment The RP-HPLC proles of the cofactor ... reaction mechanism of Crotalus adamanteus L -amino acid oxidase J Biol Chem 242, 12591264 Porter DJT & Bright HJ (1980) Interpretation of the pH dependence of avin reduction in the L -amino acid oxidase ... that the presence of 0.1% SDS did not affect the spectrum of FAD Identication of cofactor The absorption spectra between 300 and 600 nm of holo-LAAO from the active fractions of Sephadex G100...

Ngày tải lên: 07/03/2014, 05:20

... Uptake of L-[3H]proline (100 lM) was measured at pH 6.5 for 10 in the presence of unlabeled amino acids and derivatives at a fixed concentration of 10 mM and are given as percentage inhibition of ... acid L-Norvaline L-Norleucine Elongation of the backbone b-Alanine c-Aminobutyric acid d-Aminopentanoic acid e-Aminohexanoic acid O-Methyl substitution O-Methyl-glycine O-Methyl-alanine N-Methylated ... the absence of inhibiton Data are means ± SEM (n ¼ 8–10) ND, not determined Elongation of the side chain glycine L-Alanine L-a-Aminobutyric acid L-Norvaline L-Norleucine Elongation of the backbone...

Ngày tải lên: 07/03/2014, 16:20

... Local stability and key acidic amino acids in staphylococcal nuclease by the interactions of E75 with H121 and K97 It is believed that a small number of key amino acid segments play major roles ... Chen et al Local stability and key acidic amino acids in staphylococcal nuclease Fig Steady-state fluorescent spectra of wild-type and mutants of SNase Spectra of nine proteins (WT, E73G, E75G, ... processed by the addition of 2.5 protons and both E75 and E129 are the targets of protonation It is of interest to determine which other amino acids these glutamic acid residues at positions...

Ngày tải lên: 07/03/2014, 21:20

... three-dimensional structures of a -amino acids and those of b -amino acids and posed the following questions Which structures of b -amino acids overlap the canonical structures of a -amino acids? Subsequently, ... b -amino acid [19], whereas the introduction of two adjacent b -amino acids induced a 10-membered hydrogen-bonded turn (C10) analogous to the b-turn made of a -amino acids [20] This propensity of b -amino ... overlap any conformation of a -amino acids In order to visualize on the potential energy surfaces the conformers of b -amino acids that fit with canonical conformations of a -amino acids, two (/ ) w) Ramachandran...

Ngày tải lên: 08/03/2014, 08:20