agez the region of interest roi and modified exclusion zone values

... polymorphism and go to Step 3; otherwise, go to Step If the first base upstream of the region and the last base in the region not match, record their positions as the locations of the donor and acceptor ... the ends of the region, record the boundary positions as the locations of the donor and acceptor splice sites and go to step 8; otherwise, move the start and finish positions of the region one ... sites and go to step Otherwise, while the first base upstream of the region and the last base in the region match, perform the following consensus test: if the pattern GU AG or AU AC is found at the...

Ngày tải lên: 14/08/2014, 16:20

... including the absence of the ψ RNA packaging signal and a deletion preventing Env expression [16] HPNd is transcribed from the CMV promoter but because of the presence of the HIV-1 TAR and RRE, ... transfection of the indicated plasmids and treatment with actinomycin D for the indicated times Quantitation of the changes in accumulation of the spliced (B) viral mRNA species after the addition of actinomycin ... within exons and would increase inclusion of these exons and act to prevent accumulation of excessive levels of vif and vpr mRNAs A second possibility to explain the presence of exons and is that...

Ngày tải lên: 13/08/2014, 09:21

... overview of HIV-1 splice sites and their strength Schematic overview of HIV-1 splice sites and their strength (A) Schematic drawing of the HIV-1 pre-mRNA and the distribution of the splice sites The ... AG-dinucleotide) and the cis-acting elements contribute to the overall strength of the different HIV-1 3'ss and their response to Rev To evaluate the impact on the intrinsic strength of the 3'ss, i.e., the ... of this ESE may be sufficient to influence the strength of at least one of the 3'ss A4c, A4a and A4b (A4cabex) The result showed that in the absence of the bidirectional ESE in exon none of these...

Ngày tải lên: 13/08/2014, 09:20

... both the strength and cell type specificity of disease induction [7,8] Within the LTR the specificity has been located mainly to the enhancer region in U3, and further narrowed down to the sequences ... signify the PCR primers used to verify the stability of the introduced mutations Lower panel shows the positions and types of the introduced mutations, marked by asterisks and underlined The SA'/SD'-delineated ... by the box The boldfaced A in the sequence indicates the presumed branch point capsid gene, the mutations affect the branch point site, the pyrimidine region, the conserved splice junction AG and...

Ngày tải lên: 13/08/2014, 05:22

... within the Env coding region Characterization of the rtm ORF The splice junction between the SD6140 and SA8514 sites predicted the existence of a novel ORF in which the Nand C-terminal parts of the ... [13,23,24] The ratio between the 18- and 17-kDa isoforms depends on the context and/ or the level of the Rtm expression, the 18-kDa being the major product in transfected 293T cells whereas the 17kDa ... domains of the native protein Thus, the role of the cytoplasmic domain of TM expressed in these two similar hybrid proteins would be interesting to examine in the context of the high variation of...

Ngày tải lên: 13/08/2014, 06:20

... whether undiscovered SNPs, or even inaccuracies in the available human genome sequence, may explain some of the remaining candidates The genomic sequence of the splice site regions of GNAS and ... length of the affected as well as the downstream introns, and length of the upstream and downstream exons (data not shown) TG 3' splice sites are significantly often found in the first intron of the ... summary, of the 39 RefSeqs showed robust support for unusual TG acceptor sites (Table 1, entries in bold), and of these overlap with cases obtained from the EST screening approach while the others...

Ngày tải lên: 14/08/2014, 08:20

... authority on other departments They have all the accountability and none of the power They must convince these departments because they can never “force” their ideas Therefore, they must have ... reviews the opportunities and threats of outside factors that can affect the future of the business The results of the SWOT analysis define the main issues to be addressed in the plan The marketing ... objectives they want to achieve, their strategies to achieve them, the resources required, and the expected results The operational marketing plan is the detailed scheduling and budgeting of the actions...

Ngày tải lên: 24/10/2013, 07:20

... Creating the Home Page for Institute of Art ! Create a home page ! Create the home page for the Institute of Art The home page for the site will display the following text as the profile of the institute: ... institute: The Institute of Art assures the highest standard of presentation and content We are continually developing a range of courses to meet the professional and creative needs of the community ... telephone, and the e-mail address of the user and the course that the user is applying for When a user submits the registration form, the data is stored in a database maintained by the institute...

Ngày tải lên: 11/12/2013, 14:15

... • Financial projections and plans • The market and the customers • Risk analysis • The industry and competition • Technology analysis • The specifics of the products and/ or services 31 Storefront ... to one another for the purpose of exchanging information 21 e-Business and e-Commerce Overview • Successful e-businesses are those that recognize the needs of their target audiences and match ... Transitioning to the Web • The Internet has changed the way people communicate, conduct business and manage their daily lives • Technologies reviewed • Resources used History of the World Wide...

Ngày tải lên: 16/01/2014, 17:20

... description of page, keywords and title of page • Most search engines rank your site by sending out a spider to inspect the site • The spider reads the META tags, determines the relevance of the Web ... locations and demographic profiles • Determine the level of personalization • Personalized direct e-mail targets consumers with specific information and offers by using customer names, offering the ... at the right time and sending promotions • Response rate • Shows campaign success or failure by measuring the percentage of responses generated from the target market Promotions • Online and offline...

Ngày tải lên: 16/01/2014, 17:20

... Other Web retailers integrate other models such as the auction model or price-comparison model Retailing on the Web (cont.) • E-tailers often offer lower prices to generate brand awareness and ... wholesaler, marks up the price and then offers the products to consumers • Online retailers can communicate easily with suppliers, shipping companies and customers • Most Web retailers use the shopping-cart ... questions • Health Insurance Portability and Accountability Act (HIPAA) • Requires medical offices and other healthcare related agencies to perform many of their administrative tasks online • Examples:...

Ngày tải lên: 16/01/2014, 17:20

... walk in the park The birds were singing and the kids were all back at school. If the code may be found in the book’s code archive, the name of the file will appear at the top of the program ... is dedicated to making the world a better place through the technical editing of SitePoint books, kits, and articles He is also a busy father of five, enjoys coffee, and often neglects his blog ... 978-0-9804552-1-2 Printed and bound in the United States of America v About the Authors Cristian Darie is a software engineer with experience in a wide range of modern technologies, and the author of numerous...

Ngày tải lên: 14/02/2014, 10:20

... (Vol of 3), by John Morley 13 This arrangement of the sciences and the Law of the Three States are together explanatory of the course of human thought and knowledge They are thus the double key of ... considerations of utility and a balance of expediencies Many of these proposals are of the highest interest, and many of them are actually available; but there does not seem to be one of them of an available ... separates the collective facts of society and history from the individual phenomena of biology; then he withdraws these collective facts from the region of external volition, and places them in the region...

Ngày tải lên: 18/02/2014, 12:20

... metal at the active site The presence of iron is therefore a prerequisite for its effect and suggests that the absence of reactivity with the MnSOD mutants is due to the lack of activity in these ... involving other residues and solvent molecules An unexpected result was the extremely high thermostability of Mn[G77Q] and the very low thermostability of Fe[A141Q] (Fig 5) Each of these mutants ... and E coli SODs with the intention of altering their metal specificities In the former cambialistic enzyme, mutations Q70G/A142Q reduced the iron-supported activity of the enzyme and altered the...

Ngày tải lên: 21/02/2014, 01:21

... dependence of the accuracy values and of the fraction of total residues with a given accuracy on the reliability index [34] of the prediction are shown in Fig It appears that 70% of the exposed ... that on average the probability of correct assignment is independent of the class type In contrast, the Q index (the number of the true positives over the number of all positives in the class) is ... in the interaction with DnaK [41], and one of the residues of the motif is also predicted by neural networks As a whole, the predicted residues indicate the expected and probable regions of interaction,...

Ngày tải lên: 21/02/2014, 15:20

... ring of the androstenedione led to a modification of the conformation of this ring We optimized the position of the ligand and the orientation of the hydrophobic group of V370 (modification of dihedral ... carried out considering the orientation of C(1), C(2) and C(19) above the heme [41] and the position of the ligand towards D309 and T310 The threedimensional structure of androstedione was obtained ... complex to the C(19) methyl of testosterone; and (d) the role of D309 and of E302 in aromatization of androgens and nor-androgens, respectively Thus, N130, located at the opposite face of D476,...

Ngày tải lên: 21/02/2014, 15:20

... features of the Mahayana are the worship of Bodhisattvas and idealist philosophy These are obviously parallel to the worship of Siva and Vishnu, and to the rise of the Vedanta Now the worship of these ... after 450 On the other hand portions of the sutra about Amợda's Paradise, of the Prajủõ-põramitõ, and of the Avatamsaka were translated about 150 A.D and translations of the Lotus and Lalita-vistara ... East[118] and was the fundamental thesis of Bodhidharma, the founder of the Zen school But the practical character of the Chinese and Japanese has led them to attach more importance to the moral and...

Ngày tải lên: 07/03/2014, 03:20

... world, the soul and God (the three terms being practically the same) have two modes of existence: one of repose and bliss, the other of struggle and trouble Of these the first mode is the better and ... the world, He is the Lord of the world and He is myself." Here the last words destroy the apparent determinism of the first part of the sentence And similarly the Chandogya Upanishad says, "They ... are further from the teaching of Gotama than the Papacy, the Inquisition, and the religion of the German Emperor, from the teaching of Christ A religion is the expression of the thought of a particular...

Ngày tải lên: 07/03/2014, 03:20

... Binding to the UAS C-2 region (in which the mutations had been placed), was impaired both at the 3¢ end of the central regions R22 and R23, whereas the region R21 and the 5¢ part of R22 were ... assembled as described in the main text Relevant restriction sites and the direction of transcription for the hbpR and the luxAB genes are indicated (A) (B) Detail of the region of the calmodulin-binding ... sequences of the palindromes [19,21], but the exact contribution of nucleotides in the binding site contacted by the proteins is not known The current hypothesis of hexameric EBR oligomers on their...

Ngày tải lên: 07/03/2014, 17:20