advances in experimental medicine and biology

... measured using endogenous substrates was performed by incubating 50 lg of protein in 30 lL of 50 mM potassium phosphate buffer, pH 7.0, containing 50 mM KCl, protease and esterase inhibitors, and [3H]AcCoA ... coincide with a margins obtained were all classified as normal by routine protein cross-reacting with an antibody directed against a 69 clinical and histological methods Histochemical or amino-acid ... carcinoma (Table 2) Interestingly, the level of Neu5,9Ac2 in the resection margin obtained from two Stage IV patients was dramatically reduced in comparison with that observed in Stage I, II and...

Ngày tải lên: 07/03/2014, 14:20

... TSK-GEL HW75-F, and each fraction was examined by Western blotting with the anti-H-chain, the anti-L-chain, or the anti-fhx/P25 Ig (Fig 1C) Protein complexes containing H- and L-chains, and fhx/P25 ... lanes and 6) and two transgenic lines [L6 · (5 lg; Fig 5, lanes and 7) and H-chain L-chain fhx/25 H-chain : L-chain : fhx/P25 L7-4 (200 lg; Fig 5, lanes and 8)] were subjected to Western blotting ... glycoprotein [28,29], in uenza virus hemagglutinin [30,31], and IgM [32] in ER, a further role of N-linked oligosacharide chains of fhx/P25 in the process of assembly of the elementary unit in ER...

Ngày tải lên: 16/03/2014, 16:20

... pyridylaminated N-glycans from squid rhodopsin and characterization by 2D mapping Rhodopsin protein (1 mg), corresponding to 20 nmol oligosaccharides, was used as the starting material All experimental ... chain within the translocon and therefore can in uence topogenesis of membrane glycoproteins [25] Molluscan rhodopsin possesses only one N-glycosylation site, whereas frog, bovine, and human (and ... innermost GlcNAc affects rhodopsin function by a local conformational change in the polypeptide chain Examination of the N-glycan structures provides insight into the processing of sugar chains...

Ngày tải lên: 23/03/2014, 17:22

... with 0.2 m KNCO After 30 and 60 min, samples were withdrawn, diluted in buffer Hydrogen bonds involving Gly372 in factor VIIa containing mgặml)1 bovine serum albumin, and the residual amidolytic ... substrate binding cleft (the 170 loop) in the active site region and the S1 pocket, whereas the intramolecular connections, for instance between the TF-binding region and the active site and between ... Hydrogen bonds involving Gly372 in factor VIIa especially in the vicinity of the S1 pocket [11,34], in the rst part of activation loop 3, and in the area of the TF-interactive helix and the 170...

Ngày tải lên: 18/02/2014, 08:20

... polyamines (putrescine, spermidine and spermine) through conversion of l-arginine into l-ornithine [16] The latter is in turn used by ODC to produce putrescine, the precursor of spermidine and ... replication [41–43] HSV inhibited polyamine biosynthesis by inhibiting protein synthesis, whereas human cytomegalovirus infection induced spermine and spermidine expression in fibroblasts [41,44] ... Sen, Lemer Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA) or anti-(a-actin) Ig (Chemicon) in blocking solution After five 5-min washes in NaCl ⁄ Pi containing 0.05% Tween 20,...

Ngày tải lên: 07/03/2014, 21:20

... functioning of the kidney, maintaining the glomerular basement membrane architecture is crucial, which in turn depends on the proper structure and function of matrix proteins, including laminin and ... [14] In this article, we have tried to determine and explore the changes in Nlinked oligosaccharides of laminin during diabetes and the likelihood of dietary fiber and butyric acid in modulating ... first step, laminin from rat kidneys from different experimental groups was purified and purity was ascertained by SDS ⁄ PAGE using silver nitrate staining (depicted in Fig S1 for laminin from starch-fed...

Ngày tải lên: 22/03/2014, 17:20

... D and Birnbaum L S TCDD-induced altered expression of growth factors may have a role in producing cleft palate and enhancing the incidence of clefts after coadministration of retinoic acid and ... 10% neutral buffered formalin For histological examination, the sections of craniofacial tissues were processed, embedded in paraffin and stained with hematoxylin and eosin (H&E) Data analysis The ... Laboratories, Inc., Andover, MA, USA, and its purity was 98 % TCDD was initially dissolved in a small volume of acetone and subsequently adjusted to a working concentration in olive oil Animals Female and...

Ngày tải lên: 07/08/2014, 14:23

... typing exit, and turn all the devices off Then remove and store the cables and adapter 4-4 CCNA 3: Switching Basics and Intermediate Routing v 3.0 - Lab 7.2.6 Copyright  2003, Cisco Systems, Inc ... command Switch_A#show spanning-tree brief b On Switch_B type show spanning-tree brief at the privileged EXEC mode prompt as follows: Switch_B#show spanning-tree brief Examine the command output and ... brief 3-4 CCNA 3: Switching Basics and Intermediate Routing v 3.0 - Lab 7.2.6 Copyright  2003, Cisco Systems, Inc c What changes have taken place in the command output? On Switch_A? ...

Ngày tải lên: 11/12/2013, 14:15

... cooperate in modulating PAI-2 mRNA stability, REMSA analyses were performed to determine the role of the ARE II and ARE IV sites in the binding of proteins to the ‘extended ARE’ Binding of cytoplasmic ... WI, USA) generating pTEasy-Globin and pTEasy-GAPDH For in vitro transcription, 500 ng of SpeI linearized pTEasy-Globin and SacII linearized pTEasy-GAPDH were incubated for h in the presence of ... analysis (10 lg of total RNA) using the intron spanning 32P-labelled globin and GAPDH probes described above The b-globin and GAPDH mRNA band intensities were visualized and quantified with a Phosphorimager...

Ngày tải lên: 16/02/2014, 09:20

... active domain homologues, in the bisphosphatase domain than in the kinase domain Conservation in the bisphosphatase domain is in the range 26–40% compared with just 13–28% in the kinase domain The ... Tb2, Lm2 and Tc2–1 PFK-2 and FBPase-2 domains in the C-terminal halves of the proteins are indicated as in Fig In the N-terminal halves, the ankyrin motifs with a primary structure having high ... for ankyrin repeat structures including the designed ankyrin protein included in the alignment The pairwise sequence identity between Lm1, Tb1 and Tc1–1 in this region is between 60 and 65%,...

Ngày tải lên: 16/03/2014, 22:20

... — Starting with the Command Line Changing How Import Works — Bringing in More Packages 112 113 115 118 118 xvii www.it-ebooks.info Contents Modules and Packages 120 Bringing Everything into the ... — Strings What is a String? Why the Quotes? Why Three Types of Quotes? Using the print() Function Understanding Different Quotes Putting Two Strings Together Joining Strings with the Print() ... Python 2.6 and Python 3.1 James Payne Wiley Publishing, Inc www.it-ebooks.info Beginning Python®: Using Python 2.6 and Python 3.1 Published by Wiley Publishing, Inc 10475 Crosspoint Boulevard Indianapolis,...

Ngày tải lên: 24/03/2014, 01:21

... metalloproteinases (MMPs) constitute a large family of proteolytic enzymes containing a metal in their organic structure playing key roles in degradation of proteins in extracellular matrix and in tissue ... C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells: implications for clinical and subclinical atherosclerosis J Am Coll Cardiol 2006, 47:1369-78 Orbe J, Fernandez ... dissection, aortic aneurysm and myocardial ischemia Atherosclerosis is the main common pathologic finding in these disorders Clinical studies have investigated the role of MMP-1 in this pathologic process...

Ngày tải lên: 10/08/2014, 10:20

... 24 post-infection, and some cells were seen at a later time point to detach into the cell medium The floating and clumping CPE morphology started to form after days post-infection The infectivity ... Significant increases (p ≤ 0.05) in cytokines between virus-infected and mockinfected cell cultures are given in Table [Bars equal standard deviation from the mean of triplicates] growth factor, is involved ... production levels comparing between DENV-infected and mock-infected HPMEC-ST1.6R endothelial cells Time (Hrs) Post-infection DENV-1 -infected DENV-2 -infected DENV-3 -infected DENV-4 -infected 0.542504...

Ngày tải lên: 12/08/2014, 04:21

... dẫn học sinh đọc tác t©m hån cđa nhµ th¬ b¹n mÊt phẩm, gọi học sinh đọc tác III Đọc- hiểu : phẩm 1.Hai câu đầu :Cảm xúc tác giả: - “BácDương …thôi rồi”:tác giả bàng hoàng nghe tin Học sinh tìm ... thiếu vắng qn đội qui -> đứng lên đánh giặc hi sinh oanh liệt Hình tượng nghĩa sĩ thể tinh thần tự giác cao độ, anh dũng vơ song làm cho kẻ địch kinh hồn, bạt vía Ai v·n: - §©y lµ tiÕng khãc lín ... theo motif có sẵn kinh nghiệm cá nhân - Ít dựa vào kinh nghiệm cá nhân nên người kể chuyện thường người biết trước, biết hết 5.Dặn dò: Soạn Luyện tập tượng tách từ 6.Rút kinh nghiệm- bổ sung:...

Ngày tải lên: 10/06/2013, 01:25

... name’s Minh d.How old is he ? He is twenty e.Where does Thuy live ? She lives in a house ( near a lake ) f.What’s there , near the house ? There is a river , a hotel , a park , a lake and a ... There is a rice paddy near the house III Home work : -Learn the new words and structure -Reread the text and write the As in your notebooks -Prepare for A 4-5  ... paddy near her house Complete the sentences : IV Completing : V Further practice : 1.Talk about Thuy’s house : This is Thuy’s house It is in the country Near her house , there is a lake , a ………...

Ngày tải lên: 22/06/2013, 01:25

... Câu2: (4đ) Kinh tế: -Phát triển chậm ,tụt xuống thứ giới sản xất công nghiệp - Một số ngành công nghiệp đời tăng trởng nhanh : đIện khí, hoá chất - Các công ty độc quyền đời chi phối kinh tế Pháp ... c, Thứ d, thứ 2, Vì nói Mỹ xứ sở ông vua công nghiệp a, Mỹ có công nghiệp phát triển b, Mỹ có kinh tế phát triển c, Mỹ có công nghiệp phát triển mạnh mẽ công ty độc quyền khổng lồ hình thành ... bảo vệ quyền lợi cho B, Đẩy mạnh sách hàng đầu giới cầm quyền Anh Câu (1 điểm): Hãy nối thông tin cột A cho phù hợp với cột B A B a Anh 1, Chủ nghĩa đế quốc thực dân b Pháp 2, Chủ nghĩa đế quốc...

Ngày tải lên: 30/06/2013, 01:28

... kinh tế , pháp luật trò, pháp luật đạo đức theo quan điểm triết học Mác-Lênin Chế độ kinh tế sở pháp luật; trò biểu tập trung kinh tế , , đường lối trò giai cấp cầm quyền trước hết thể sách kinh ... quan hệ kinh tế có nội dung pháp luật Pháp luật luôn phản ánh trình độ phát triển kinh tế, cao thấp không khác với trình độ phát triển kinh tế Ví dụ: kinh tế thò trường, quan hệ chủ thể kinh tế ... hệ pháp luật với kinh tế thể chỗ, sinh từ điều kiện, tiền đề kinh tế pháp luật không phản ánh cách thụ động mà có tác động trở lại phát triển kinh tế Pháp luật tác động đến kinh tế theo hướng...

Ngày tải lên: 09/07/2013, 01:25

Ngày tải lên: 26/07/2013, 01:25

Ngày tải lên: 04/09/2013, 12:10