adoption of therapeutic stem cell technologies by large pharmaceutical companies

... this kind of information 2.1.5 Disclosure of information of public companies under Vietnamese Accounting Standards The following are two provisions of information disclosure of public companies ... enterprises, or there are results of a separate issuing tranche by the company;  There is a decision of the general meeting of shareholders or of the board of management on dividends to be paid; ...  There is a change to any key personnel of the company (a member of the board of management, board of controllers, of the general directorate or board of directors, or chief accountant) 26 ...

Ngày tải lên: 13/03/2014, 14:20

... characteristics of stem cells: (A), Symmetric cell division leads to self-renewal of stem cells; (B), Asymmetric cell division leads to replacement of the stem cell and production of a sister cell, exemplified ... that modified stem cells could be used in cell therapy for Parkinson’s disease 42 Therapeutic Uses of Stem Cells But, as with other proposed stem- cell therapies, an autologous source of cells that ... discovery of methods to control the proliferation and differentiation of many kinds of stem cells, and technologies are already being developed for replacing the nuclei of stem cells with those of prospective...

Ngày tải lên: 11/06/2014, 12:52

... Chemomodulation of phenotype of human DCs by antineoplastic chemotherapeutic agents in low noncytotoxic concentraChemomodulation of phenotype of human DCs by antineoplastic chemotherapeutic agents ... concentrations of eight chemotherapeutic agents for human DCs Cells were treated with a range of concentrations of cytotoxic agents (0–100 nM) and the levels of apoptosis were assessed by Annexin ... activation of mitochondrial dehydrogenases in DCs, which makes the analysis of dose-dependent cell viability Chemomodulation of DC phenotype by low noncytotoxic concentrations of chemotherapeutic...

Ngày tải lên: 18/06/2014, 15:20

... on cell cycle synchronization of the tumor cell population [3] Theoretically, such synchronization is achieved when sublethal DNA damage is applied to the tumor cells, by means of activation of ... depletion of the G1 phase population of cells with functional p53 but accumulation of both G1 and G2/M phase populations of cells with defective p53 Moreover, histone acetylation was followed by significant ... of irradiated cells, irrespective of p53 status This observation is in accordance with the concept of increased probability of tumor cell death when the chromatin structure is modified Each cell...

Ngày tải lên: 09/08/2014, 10:21

... apoptotic cell death in eGFP-FMRP transfected cells compared with eGFP transfected cells was prevented by Akt inhibitor VIII as determined by the quantification of the number of PI positive cell (Figure ... level of FMRP was determined by Western blot (D) After LY treatment, cell viability was measured by MTT assay, inhibition of Akt phosphorylation further decreased cell viability (E) The level of ... indicate that induction of FMRP has a protective role in the cell and delays the onset of apoptosis by etoposide on HeLa cells Also activation of PI3K-Akt-Bcl-xL within cells harboring eGFP-FMRP...

Ngày tải lên: 10/08/2014, 05:21

... metastasis by malignant tumor cells consists of three major steps: the receptormediated adhesion of tumor cells to the extracellular matrix, the degradation of the extracellular matrix by the proteinase ... dampen the effect of activation-induced apoptosis of T cells Following stimulation with PHA for days, the rate of apoptosis of T cells was 23.37 ± 2.71% When PHA-stimulated T cells were cocultured ... endothelial cells at the single -cell level, suggesting these cells have the properties of hemangioblasts Page of 10 The main purpose of our article was to examine the immune characteristics of Flk1...

Ngày tải lên: 10/08/2014, 10:21

... trials, administered "megadose" of donor hematopoietic stem cells by combining G-CSF mobilized peripheral blood with bone marrow stem cells, both depleted of T cells, following TBI-based conditioning ... NK cell/ KIR ligand mismatching Lysis of leukemia cells by natural killer (NK) cells is mediated in part by mismatching of the killer immunoglobulin-like receptor (KIR) ligand between the NK cell ... "Megadose" stem cells have been proved to facilitate stable engraftment despite such an extensive Tcell-depleted stem cell graft by several groups for haploidentical HSCT[21,22], the rate of rejection...

Ngày tải lên: 10/08/2014, 22:20

... application of the method produces a set of 4,449 cell and tissue markers, including 45 out of 71 known stem cell markers (69%) Analysis of the markers that segregate six types of stem cells (hematopoietic, ... differentiated sets of stem cells We chose six pairs of stem cell samples and their differentiated derivatives from our dataset (two embryonic stem cell lines, hematopoietic stem cells [HSCs], osteoblasts, ... function that endows cells with the property of 'stemness', in the same way that there appears to be no single stemness gene for all stem cells [60] This set of 426 markers of stem cell differentiation...

Ngày tải lên: 14/08/2014, 08:20

... Specification of the pronephric anlage Onset of nephrogenesis Onset of cellular differentiation (b) 29/30 (35 hpf) 35/36 (50 hpf) 40 (66 hpf) Maturation and terminal differentiation Acquisition of excretory ... expression of most slc genes was evident by this stage, which preceded the onset of pronephric functions by about hours Furthermore, the stage 35/36 nephron retains a simple structure, lacking areas of ... organization Models of the segmental nephrons of the Xenopus pronephric and Models of the segmental organization of the Xenopus pronephric and mammalian metanephric nephrons The color coding of analogous...

Ngày tải lên: 14/08/2014, 08:21

... pluripotent stem cell hPST human pluripotent stem cell test IC50 the half maximal inhibitory concentration ID50 50% of inhibition of differentiation concentration iPSC induced pluripotent stem cell ... epithelial cells lose their cell polarity and cell- cell adhesion and become migratory and invasive mesenchymal cells It is important for early cell differentiation and collective cell migration ... indicated by white dotted lines in (a) Each intensity profile in (c-d) is an average of 16 intensity profiles obtained from colonies (e) Percentage of T+ colony area in different colony geometries of...

Ngày tải lên: 09/09/2015, 08:18

... perturbation of normal stem cell behavior can lead to cancer have been key areas of research in the field of stem cell biology Asymmetric stem cell division is a common strategy where different cell ... germline stem cell and its surrounding cap cells (in ovary) or hub cells (in testes) is a good example of how stem cells utilizing this mechanism establishes polarity and maintains its stem cell ... between the maintenance Introduction of stem cells and the supply of fully differentiated cells is achieved by regulating the number and the mode of stem cell division, which can be either symmetric...

Ngày tải lên: 11/09/2015, 14:32

... cells Put together, all these evidence suggest that the regulation of Lefty1 by OCT4, KLF4 and NANOG may be part of the ES cell self renewal mechanism The role of Lefty1 in regulation of ES cell ... seeding densities of 300 and 500 cells per 56cm2 (surface area of each well of a well dish is 56cm2) When 300 cells were reseeded, the number of secondary colonies formed for cells transfected ... attenuation of Oct4 promoter activity by 18% while shRNA3 caused a more dramatic reduction of 52% (Figure 5) The phenotypic effect of Lefty2 RNAi on ES cells was then investigated By the third day of...

Ngày tải lên: 11/09/2015, 16:02

... development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated A part of this thesis ... Conflicting roles of Nodal signaling in both the maintenance of ES cell self renewal and induction of ES cell differentiation have both previously been reported A key objective of this thesis is ... specification of ES cells is a SMAD3 dependent process On the other hand, SMAD3 plays no role in trophectodermal 158 specification of ES cells as inhibition of SMAD3 activity does not lead to ES cell...

Ngày tải lên: 11/09/2015, 16:02

... ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For transfection of ... day of selection 4.9 Alkaline phosphatase staining Detection of alkaline phosphatase, which is indicative of the nondifferentiated state of ES cells, was carried out using a commercial ES Cell ... 4.14.1 Enhancement of Nodal/Activin signaling E14, TAG1 or tet-Nodal E14tg2a cells were seeded in ES cell medium at a density of 676 cells/cm2 without gelatin The next day, the seeded cells were subjected...

Ngày tải lên: 11/09/2015, 16:02

... pluripotency of human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells ... expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self-renewal of teratocarcinoma and embryonic stem cells Oncogene ... fails to maintain self-renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and Benvenisty, N (2006) Overexpression of NANOG in human ES cells enables feeder-free growth...

Ngày tải lên: 11/09/2015, 16:02

... key regulators of ES cell fate These factors are at the top of the hierarchy of the ES cell regulatory network and they keep ES cells undifferentiated by either the activation of target genes ... lines of evidence that Nodal signaling mediated by SMAD2/3 indeed plays a role in self renewal and cell fate decisions of pluripotent cell populations in vivo and also that of embryonic stem cells ... layer of fibroblast in the presence of serum An indication that fibroblasts act by secreting a signal that inhibits ES cell differentiation was substantiated by the ability of Buffalo rat liver cell...

Ngày tải lên: 11/09/2015, 16:02

... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells and differentiated cells ... Subunit of Cadherin protein complex Intracellular mediator of canonical Wnt signaling Non-SET domain protein Histone H3 methyltransferase Transcription factor Intracellular mediator of canonical...

Ngày tải lên: 11/09/2015, 16:03

... cancer stem- cell concept, tumours are not merely simple monoclonal expansions of transformed cells, but complex tissues where 12 abnormal growth is driven by a minority of cancer stem cells (Boman ... progression of cells from G1 into S phase in the cell cycle Increased expression of p27 in mammalian cells 20 induces a G1 block of the cell cycle (Hengst et al., 1996) The high expression levels of p27 ... A simplified model of malignant transformation based on the cancer stem cell concept This model describes the transformation of a tumour cell from a normal cell as proposed by Polyak and Han (Polyak...

Ngày tải lên: 03/10/2015, 20:57

... mediated by NSC-derived neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex of E16 Sprague-Dawley ... ventricles of ischemic rats with and without NGF administration or neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic ... of bone marrow stem cells in ischemic brain Acta Neurochir Suppl 2006, 99:123-124 Li J, Sun CR, Zhang H, Tsang KS, Li JH, Zhang SD, An YH: Cotransplantation of neural stem cells and Schwann Cells...

Ngày tải lên: 18/06/2014, 16:20

... transplantation of hMSCs via tail vein could ameliorate the deterioration of motor function in SCA2 transgenic mice Rescue of Purkinje Cells by Transplanted hMSCs Loss of PCs had been noted by immunohistochemical ... onset of motor function loss by better Page of engraftment of hMSCs in brain tissues and rescuing cerebellar PCs from cell death, possibly through release of neurotrophic factors or direct cell ... Effects of Umbilical Cord Blood-Derived Mesenchymal Stem Cell Transplantation in Experimental Lupus Nephritis Cell Transplant 2011, 20:245-257 32 Parekkadan B, Milwid JM: Mesenchymal stem cells as therapeutics...

Ngày tải lên: 10/08/2014, 10:20