adipose stem cell engineering clinical applications in plastic and reconstructive surgery

... Material Engineering Chapter 6: Applications in Medical and Biology Magnetic Material Engineering Chapter 6: Applications in Medical and Biology Magnetic Material Engineering Magnetic Cell Separation ... Material Engineering Chapter 6: Applications in Medical and Biology Magnetic Material Engineering Magnetic nanoparticles Chapter 6: Applications in Medical and Biology Magnetic Material Engineering ... / Cell Labeling Diagram of Immunomagnetically labeled Cell Chapter 6: Applications in Medical and Biology Magnetic Material Engineering Magnetic Cell Separation / Cell Labeling Chapter 6: Applications...

Ngày tải lên: 30/03/2014, 11:20

... suggested 30 Biomedical Engineering – Technical Applications in Medicine that albumin is released by osteoblast cells present in fracture healing sites and this excess albumin increases proliferation ... basis of new applications of technics, mathematics and physics into medicine, biomedical engineering has been gradually formed and it is defined as an interdisciplinary science discipline that uses ... complete at least one missing unknown quantity in the metaphoric formula in their research and thus will gain new valuable information I wish you a pleasant and inspiring reading Ing Radovan Hudak,...

Ngày tải lên: 28/06/2014, 10:20

... Akkina and Jennifer Quick for help with maintaining hES cells and culturing embryoid bodies We thank Leila Remling for isolating fetal CD34 cells We thank 19 20 21 22 23 Weissman I: Stem Cell ... trypsin/EDTA supplemented with 2% chick serum (Invitrogen, Carlsbad, CA) for 20 minutes at 37°C Cells were washed twice with PBS and filtered through a 70 uM cell strainer to obtain a single cell ... cell lines using modular lentivectors Stem cells 2005 in press Pfeifer A, Ikawa M, Dayn Y, Verma IM: Transgenesis bylentiviral vectors: lack of gene silencing in mammalian embryonicstem cells and...

Ngày tải lên: 13/08/2014, 09:21

... have interactions with both injured tissues and transplanted stem cells, which may affect their behaviors Moreover, in in vivo the blood circulation and joint motion may have some role in stem cell ... Buschmann MD Fibronectin, vitronectin, and collagen I induce chemotaxis and haptotaxis of human and rabbit mesenchymal stem cells in a standardized transmembrane assay Stem Cells Dev 2007 Jun;16(3):489-502 ... mesenchymal stem cell implantation of femoral condyle Immunohistochemistry (IHC) staining against collagen I (A); IHC against collagen II (B); IHC against collagen X (C); and Alcian blue staining against...

Ngày tải lên: 10/09/2015, 09:01

... 1.11 Cellular MRI MRI is a practical imaging tool used to track administered cells in studies for tissue engineering, cellular transplantation, and homing and migration of cells In tissue engineering, ... Hering) Oval cells that subsequently generate hepatocytes and ductular cells Pancreatic stem cells Intraislet, nestin-positive cells, oval cells, duct cells Beta cells Skletal-muscle stem cells ... basal and mucussecreting cells, bronchiolar Clara cells, alveolar type II pneumocyte Mucous and ciliated cells, type I and II pneumocytes Stem cells of the intestinal epithelium Epithelial cells...

Ngày tải lên: 10/09/2015, 09:10

... determined using 0.3% oil red O stain for 15 minutes at room temperature to stain intracellular lipids, and counterstained with hematoxylin Osteogenic differentiation was evaluated using Alizarin ... immunohistochemical staining, sections of labeled and unlabeled pellets were fixed on slides, and were pre-digested with pepsin (1 mg/ml in Tris–HCl, pH 2.0) for 30 minutes at room temperature, and incubated ... biotinylated goat anti-mouse for 30 minutes and after washing samples were incubated with streptavidin peroxidase for 45 minutes at room temperature Sections were visualized using DAB chromogen and...

Ngày tải lên: 10/09/2015, 09:10

... Alizarin red staining showed that MSCs labeled with SPIO did not affect osteogenic differentiation capacity of cells (figure 3.10)   Figure 3-10 Alizarin Red staining Alizarin Red staining of ... Immunohistochemical staining after high density pellet culturing showed that the distribution of collagen type II in extracellular matrix of cells was same between unlabeled and 25 and 50 µg/mL SPIO labeling, ... exhibited no difference in developing fat vacuoles (Figure 3.9) 64     Figure 3-9 Oil red O staining Oil red O staining of the MSCs after 21 days adipogenic differentiation induction; Unlabeled...

Ngày tải lên: 10/09/2015, 09:11

... labeled cells migrated to different locations over time including cartilage defect, injection site, surgery scar, and supra-patellar fat and the cartilage defect site It is likely that the healing ... process and the secretion of proinflammation factors associated with remodeling and repair of the injury provided the stimuli for MSC migration MRI revealed that the injected cells were in defect ... site, para-patellar fat and injection site of the knee joint (figure 3.15 and 3.16) 71     Figure 3-15 MR imaging of the mini-pig's femoral condyle MR imaging of the mini-pig’s femoral condyle...

Ngày tải lên: 10/09/2015, 09:11

Ngày tải lên: 10/09/2015, 09:11

... migration and localization into different site of the inflammation was observed in the knee joint over time A majority of the cells moved to the surgery sites such as surgery scar, para-patellar fat and ... cells can be a promising tool for real time evaluation and tracking of MSCs, providing data for a better understanding of the migration and localization of the injected cells 76   ... and injection site, confirming the report of Jing et al (206, 207) In addition, our results showed that we could detect labeled cells in vivo up to weeks, thus the SPIO labeling method of the cells...

Ngày tải lên: 10/09/2015, 09:11

... Ibsp Chemokine (C-X-C motif) ligand 12 Interleukin 1alpha Interleukin 1beta Interleukin Tumor necrosis factor Transforming growth factor beta Transforming growth factor beta Transforming growth ... the homing and integration of the cells to the injured tissue Although there are speculations that other injured tissues express ligands and chemotactic factors that encourage homing of cells ... confluency (around 2x106 cells in a T175 culture flask), then, they detached by using TrypLE (Gibco BRL, Grand Island, NY, US) and sub-cultured into two new flasks Cells were expanded and sub-cultured...

Ngày tải lên: 10/09/2015, 09:11

... crucial in making the clinical diagnosis The majority of diagnoses are made through visual inspection This atlas is a collection of clinical photographs depicting clinical findings which warrant a plastic ... Clinical Diagnosis in Plastic Surgery Ron Hazani Mohamed Amir Mrad David Tauber • Jason Ulm Alan Yan • Michael J Yaremchuk Editors Clinical Diagnosis in Plastic Surgery Editors ... Pokhrel, MD Division of Plastic Surgery, Department of Surgery, Kleinert-Kutz Hand Surgery, Louisville, KY, USA Tahira I Prendergast, MD Division of Plastic and Reconstructive Surgery, Medical University...

Ngày tải lên: 07/07/2016, 18:56

... times between starting at the point x0 y0 and landing in the treacle The function ‘stats[random,normald](1)’ creates a random standard normal deviate (b) Plot a histogram showing the distribution ... random number The seed is set using the command ‘randomize(integer)’ before invoking ‘rand()’ Maple also provides another U generator This is ‘stats[random,uniform] (1)’ This is based upon ‘rand’ ... in Figure 2.2 Finally, each lattice can be constructed from a reduced basis consisting of vectors e1 and e2 which define the smallest lattice cell In Figure 2.1 this is long and thin, while in...

Ngày tải lên: 07/03/2014, 15:20

... molecular cloning techniques for obtaining and characterizing purified animal proteins involved in cancer and aspects of cell biology The proteins thus characterized include human cathepsins (Wen ... Victor and Sloane, 2007)] Cathepsins are classified mechanistically into groups which include serine (cathepsins A and G), aspartic (cathepsins D and E), and cysteine cathepsins (cathepsins B, ... immunoassay that demands binding of only one antibody molecule E coli colonies containing recombinant plasmids are lysed in situ, and proteins in the lysate are immobilized by binding directly to...

Ngày tải lên: 24/03/2014, 02:21

... visualizing results, improving basic submodels, finding new, interdisciplinary applications, exploring new concepts, interpreting observations in a more generalized form, or even pushing the ... complicated and interdisciplinary tasks, tomorrow’s engineers are facing (see NAS “The Engineers of 2020” at http:// national-academics.org) Also, an increasing number of engineering firms recruit ... other sciences and disciplines of engineering The subject of fluid mechanics will be highly relevant in domains such as chemical, metallurgical, biological and ecological engineering This series...

Ngày tải lên: 02/04/2014, 14:53

... experience and knowledge in evaluating and using any information, methods, compounds, or experiments described herein In using such information or methods they should be mindful of their own safety and ... biotech and process industry Jianquan Luo, MEng, Institute of Process Engineering, Chinese Academy of Sciences Mr Luo is an experienced engineer specializing in membrane applications in bioseparation ... Biopharmaceuticals and Regenerative Medicine Yinhua Wan, DPhil, is a Professor of Biochemical Engineering at Institute of Process Engineering, Chinese Academy of Sciences He has published more than 80 papers in...

Ngày tải lên: 02/04/2014, 15:04

... different cells (D) These cells, grown from the neural retina, show staining common to brain neural stem cells (DCX, red) and staining found only in neural stem cells derived from the retina (recoverin, ... vimentin (green), and nestin (blue) staining in a neurosphere demonstrate the intimate commingling of the cells in a sphere as well as expression of multiple markers both in the same cells and in ... stem cell in a fly embryo, showing the opposite localizations for ACD determinants in the stem cell and differentiating cell The Miranda protein, stained red, marks the basal complex that determines...

Ngày tải lên: 11/06/2014, 12:52

... ring cell carcinomas Cancer Res 1993, 53:3078-84 29 Weidmann E, Elder EM, Trucco M, Lotze MT, Whiteside TL: Usage of T -cell receptor V beta chain genes in fresh and cultured tumor-infiltrating ... carcinoma patients, unaffected colonic mucosa, carcinoma tissue) one has to keep in mind the differences in sample sizes and applied tests The highly significant difference in CD between blood and ... origin, we found a negative correlation between HAC/PBGD and CD (Figure 3) as well as HAC/PBGD and n(F) (not shown) However, separate analysis of the samples originating from blood and originating...

Ngày tải lên: 18/06/2014, 16:20

... Printed in Croatia A free online edition of this book is available at www.intechopen.com Additional hard copies can be obtained from orders@intechweb.org Lasers – Applications in Science and Industry, ... Applications in Science and Industry temperature of the ZnSe crystals increases In the case of pulse lasers, an increases in the transient temperature ΔT in materials can be estimated according ... structures in nano-scale, and thin films Others allowed better understanding of laser-matter interaction when sample is subjected to intense laser pulses of various time duration, shape in space and...

Ngày tải lên: 18/06/2014, 22:20

... hot spots in the beams 28 Lasers – Applications in Science and Industry Depositing high LIDT coatings at Sandia’s large optics coating operation Coating large optics goes hand in hand with large ... this chapter Regarding the polishing and preparing of optics for coating, we have demonstrated in the case of an AR coating that using one combination of polishing compound and wash preparation ... preparation of large optics for coating, including the polishing and washing and cleaning of the substrate surfaces, in ways that insure the highest LIDTs of coatings on those surfaces We turn next...

Ngày tải lên: 18/06/2014, 22:20