adhesion proliferation and osteogenic differentiation

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Ngày tải lên: 07/08/2014, 18:21

... and ubiquitin C-terminal hydrolase (specific markers for neural and neuroendocrine tissues) were found in rat long bones at embryonic day 15, in the diaphyseal and metaphyseal perichondrium, and ... adrenergic and cholinergic in nature, and originate from paravertebral sympathetic ganglia [16] Experimental nerve deletion and immunohistochemistry analysis have shown that both myelinated and unmyelinated ... Increased TBV Increased TBV and CBV Normal Normal 25 45,51 Y2) ⁄ )ob) ⁄ ) Leptin and Y2 Increased Increased Y1) ⁄ ) Y1 Decreased TBV and CBV in relation to Y2) ⁄ ) Increased TBV and CBV ND Increased...

Ngày tải lên: 18/02/2014, 04:20

... stabilizes pH and osmolarity and supplements the cells with trace compounds and energy sources (pyruvate and glucose) The added serum contains high levels of growth factors released by cells and platelets ... The cells from the unaffected and affected sides expanded with, on average, 0.21 and 0.22 cell doublings per day, respectively After one passage (4.3 cell doublings) and weeks of culture, 106 primary ... implant has to withstand mechanical stress is that systematic redifferentiation signalling, as part of the disease condition, could be impaired within the OA joint Redifferentiation and proteoglycan...

Ngày tải lên: 09/08/2014, 06:22

... fibronectin and laminin on cell retention and rate of osteogenic expression Cell Transplant 1992, 1:23-32 24 Meyer U, Meyer T, Jones DB: Attachment kinetics, proliferation rates and vinculin ... In vitro differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10:1518-1525 17 Chaudhry GR, Yao D, Smith A, Hussain A: Osteogenic ... [11] It is well known that many functions of the cells, e.g differentiation and proliferation, rely on intact cell-cell interactions and a tight attachment to extracellular matrix components In...

Ngày tải lên: 11/08/2014, 20:21

... mixed and left to stand at room temperature for 5-10 minutes The organic and aqueous phases were separated by adding 240μl of chloroform and 240 μl of 2N KCl The mixture was then vortexed, and ... concentrations tested (10μM, 25μM, 50μM, 75μM, and 100μM), none of the six compounds inhibited DAGK and human PKC alpha (Figure 2.8 and Figure 2.10) On the other hand, and in agreement with previous findings ... (10μM, 25μM, 50μM, 75μM, and 100μM) tested 54 Chapter2 Development and Evaluation of Human SPHK Inhibitors * * Figure 2.8 Effects of DMS and compounds on DAGK activity DMS and six compounds (CP1-CP6)...

Ngày tải lên: 11/09/2015, 16:06

... BM-MSCs and AD-MSCs differentiation, and the non-specific SPHK inhibitor, DMS, was used as a control Two differentiation pathways were chosen to study, namely, osteogenic and adipogenic differentiation, ... experiments using human BM- and AD- MSCs 3.1.1.4 Stem Cells Osteogenic and Adipogenic Induction Culture Condition Normal growth Adipogenic differentiation Osteogenic differentiation Culture media ... Function of CP6 and DMS in Human BM- and AD- MSCs Differentiation Human BM- and AD- MSCs are multipotent cells that are capable of differentiating along osteogenic, chondrogenic, and adipogenic...

Ngày tải lên: 11/09/2015, 16:06

... human BM- and AD- MSCs proliferation Figure 4.6 Function of S1P and serum in human BM- and AD- MSCs proliferation Human BM -and AD- MSCs were cultured in different conditions for days, and cell ... medium); and a group that received the differentiation cocktail, named the osteogenic- induced group (cultured in osteogenic differentiation media (section 3.1.1.4 Stem Cells Osteogenic and Adipogenic ... BM- and AD- MSCs proliferation Human BM- and AD- MSCs were cultured in different culture conditions and cells proliferation were tested by PicoGreen assay Results are the average + the standard...

Ngày tải lên: 11/09/2015, 16:06

... the osteogenic differentiation and adipogenic differentiation in human MSCs differently In human BM- and AD- MSCs osteogenic differentiation, 0.5μM CP6 and DMS showed to promote the osteogenic differentiation ... MSCs osteogenic and adipogenic differentiation switching To our knowledge, this is the first report on inhibitors of SPHK mediating human BMand AD- MSCs differentiation into osteogenic and adipogenic ... amounts (1μM DMS and 5μM CP6) showed to inhibit the osteogenic differentiation in most of the experiments Interestingly, in human BM- and AD- MSCs adipogenic differentiation, DMS and CP6 showed...

Ngày tải lên: 11/09/2015, 16:06

... Tissue and Cell 37:325-34 Chastain, S R., A K Kundu, et al (2006) "Adhesion of mesenchymal stem cells to polymer scaffolds occurs via distinct ECM ligands and controls their osteogenic differentiation. " ... two psoriasis susceptibility loci (HLA and 17q) and two novel candidate regions (16q and 20p) by genome-wide scan." Hum Mol Genet 6(8): 1349-56 Nirmalanandhan, V S., M Rao, et al (2007) "Effect ... sphingosine-1-phosphate and promotes cell growth and survival." J Cell Biol 147(3): 545-58 Olivera, A and S Spiegel (1993) "Sphingosine-1-phosphate as second messenger in cell proliferation induced by PDGF and FCS...

Ngày tải lên: 11/09/2015, 16:06

... stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human adult stem cell proliferation and differentiation More details about the research goals and strategies ... expansion and differentiation are addressed, including their advantages and their potential shortcomings 1.4.5 Current Strategies in MSCs Proliferation and Differentiation 1.4.5.1 Serum and Growth ... pathogenic processes as discussed above, recently, SPHK and S1P have been suggested to be involved in stem cell proliferation and differentiation 1.3 SPHK AND S1P REGULATION IN STEM CELL RESEARCH As was...

Ngày tải lên: 11/09/2015, 16:06

... skin, proliferation is tightly linked to adhesion to the basal lamina and Myc disrupts this adhesion; therefore, keratinocytes respond to Myc with premature differentiation and arrest of proliferation ... proliferation (Gandarillas and Watt 1997) The frequent activation of MYC genes in tumorigenesis has suggested that Myc proteins might also have an important function in normal proliferation and growth and ... of Myc in cell proliferation and their proposed functions Control of Cell Proliferation and Growth by Myc Proteins 333 2003) There is clear evidence that this effect is direct and not mediated...

Ngày tải lên: 25/10/2013, 21:20

... a-defensin-1 and a-defensin-2 induced increases in the phosphorylation of GSK3b and b-catenin activation and that inhibition of b-catenin activation prevented a-defensin-induced proliferation and collagen ... Results a-defensin-1 and a-defensin-2 increased proliferation and collagen synthesis in HFL-1 lung fibroblasts To study the effects of a-defensin-1 and a-defensin-2 on lung fibroblast proliferation, ... b-catenin, total b-catenin and collagen-I, and cell proliferation were determined As shown in Figs 7A,B and 8A,B, the incubation of lung fibroblasts with a-defensin-1 and a-defensin-2 induced increases...

Ngày tải lên: 18/02/2014, 06:20

... pg/well) and the intra- and inter-assay coefficients of variation were less than 7% and 9% respectively The progesterone detection limit was 12 pg/tube (60 pg/ well), and the intra- and inter-assay ... numbers, and RGD on subpyknotic anti-α6 different ratios follicles culturedpeptides Effect of6rates ofIgG on cellsmall of LNproliferation and Effect of anti-α6 IgG on cell numbers, proliferation and ... round cells and the proliferation rate of GC from small follicles (r = 0.87, p < 0.001) on the one hand (Fig 8a), and the pyknotic rate of GC from small follicles (r = 0.76, p < 0.05) and large...

Ngày tải lên: 05/03/2014, 17:20

... papillomas and carcinomas by dehydroepiandrosterone and 3-b-methylandrost-5-en-17-one in mice Cancer Res 45, 164–166 Schwartz A & Pashko L (1995) Cancer prevention with dehydroepiandrosterone and non-androgenic ... the inhibition of proliferation induced by DHEA is independent of its conversion to estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, ... R ˜ (2005) Dehydroepiandrosterone inhibits the proliferation of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and is androgen- and estrogen-receptor...

Ngày tải lên: 16/03/2014, 00:20

... ›Akt Survival, proliferation [67] Actin reorganization and cell migration Proliferation Cell growth, proliferation [33] Survival Mucin secretion Follicular survival and development Proliferation, ... survival, proliferation [45] ›G1-S phase transition Survival, proliferation ›G1 cyclins, flp27Kip1, flp21Cip1, proliferation Survival [46] [25] [52] ›Cyclin D1, ›Cyclin E, ›DNA synthesis, proliferation ... ›p27Kip1 ›PI3K ⁄ ›Akt ⁄ ›p70S6K Survival Anti -proliferation Anti-inflammatory effects, antiproliferation ›DNA synthesis, proliferation Survival Survival, proliferation Dopamine-induced neurological...

Ngày tải lên: 16/03/2014, 05:20

... treatments are: untreated control (A), 50 lM MIBA (B) and MIBG (C), 100 lM MIBA (D) and MIBG (E), 200 lM MIBA (F) and MIBG (G), and 500 lM MIBA (H) and MIBG (I) Magnification, 120 · Fig Sensitivity ... the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... electrophoresis in 1.7% agarose gels and visualized with SYBR Green I Data measurement and statistical analysis Radiotracer, cell number and enzyme assay data were quantified and plotted as means ± SEM...

Ngày tải lên: 17/03/2014, 09:20

... the adhesion deficit This concordance of apoptosis and adhesion suggests that one may cause the other In the proliferation studies we used cells transfected with V5–His fusion constructs and compared ... peptidases, DP8 and DP9 Table Cell proliferation A standard thymidine uptake assay was used Results are expressed as a proliferation quotient, which is the ratio of countsÆmin)1 of transfected and untransfected ... integrin-independent cell adhesion molecule DPIV reduces cell adhesion by dephosphorylating p38 MAP kinase and b1-integrin [29], so the effects of DP8 and DP9 on p38, b1-integrin and DDR1 phosphorylation...

Ngày tải lên: 23/03/2014, 11:20

... factor and the loss of cell–matrix and cell– cell contact is a characteristic feature of apoptosis [16] Focal adhesion complexes consist of integrin proteins, the tyrosine kinases Src and focal adhesion ... least 11 isoforms of PKC have been identified and can be classified into conventional (a, bI, bII and c), novel (d, e, g, and h), atypical (f and k ⁄ i) and novel ⁄ atypical (l) based upon their cofactor ... per lane) were subjected to SDS ⁄ PAGE and western blotting using specific antibodies against p130Cas (A and B), PKCd (C and D, upper panel) and actin (C and D, lower panel) as described Molecular...

Ngày tải lên: 23/03/2014, 11:20

... relationship between practical adhesion energy and the work of adhesion is the generalized fracture mechanics theory of Gent and coworkers [23-251 and contributed to by Andrews and Kinloch [26-291 This ... practical adhesion and the work of adhesion, W, Assuming the work of adhesion to be measurable, one must next ask if it can be related to practical adhesion If so, it may be a useful predictor of adhesion ... powdered solids and give us an in-depth view of the types of intermolecular forces that control adhesion of solid surfaces The chapter by Wahl and Syed Asif explores the behavior of adhesion and contact...

Ngày tải lên: 26/04/2014, 15:36

... TJ and MZ conceived the study and wrote the manuscript TJ designed and performed tissue culture, Western Blotting and Immunofluorescence MS, NMV and EZ helped with flow cytometric evaluation and ... patient, normal muscle cells and co-cultures were trypsinized by standard procedures, washed with PBS 1× and centrifuged for minutes at 1,400 rpm CD34+ cells were washed and centrifuged with PBS ... cells and DMD myoblasts a) after hour (630×); b and c) after 24 hours (200×) arrow indicate syncytium Microscope Zeiss Axiovert 200 diac and skeletal muscle or purkinje cells On the other hand,...

Ngày tải lên: 18/06/2014, 15:20