active supervision and review are provided for all personnel

... NAGases are not essential for normal growth on non-chitinous carbon sources Dnag2 strains C2332 and A523 and Dnag1Dnag2 strains 713 and 1921 were randomly chosen and used together with the WT and ... subsequent experiments for thorough characterization of their phenotypes Dnag2-I and Dnag2-II are Dnag2 strains C2332 and A523, and nag1Dnag2-I and nag1Dnag2-II are Dnag1Dnag2 strains 713 and 1921, respectively ... normal growth and sporulation of the knockout strains, and (C) minimal medium with chitobiose Dnag2-I and Dnag2-II are Dnag2 strains C2332 and A523, and nag1Dnag2-I and nag1Dnag2II are Dnag1Dnag2...

Ngày tải lên: 23/03/2014, 05:22

... Manager owns leadership skills are able to motivate work groups to achieve all the goals for that group and thus improve the overall quality of workplace experience for all employees Motivation Motivation ... schedule, organize and follow your own plan • • Organizational skills create structure and eliminate chaos Essentially, they help you focus on what Organizational skills create structure and eliminate ... can become complacent and sometimes bored when they the same job day in and day out, so managers have to be motivators Dealing with changes Managers need to prepare yourself for the necessary knowledge...

Ngày tải lên: 08/07/2014, 10:33

... glufosinate and used for further analyses Microscopy For observation of chloroplast size, tips from expanding leaves were cut and fixed with 3.5% glutaraldehyde in water for h at room temperature and ... proteins, ptCpn60α and ptCpn60β, both of which are expressed in all tissues [31,38] Despite the difference, our results showed that both ptCpn60α and ptCpn60β are required for plastid division ... indicated that there are two types of cyanobacterial chaperonin 60 proteins, GroEL-1 and GroEL-2, and that only one group, GroEL-1, gave rise to ptCpn60α and ptCpn60β in land plants and green algae...

Ngày tải lên: 12/08/2014, 03:20

... flexibility and social knowledge and experience of managers The objective of this skill is to enhance mutual understanding and respect on the basis of identification and recognition of the values and ... Ensure the safety and peace of colleagues • Create favorable conditions for joint work • Evoke and establish collective spirit • Convey understanding, experience • Wise in the handling of documents ... lead, motivate, inspire, and encourage them… • Good managerial skills are all about planning and utilising time, resources and man power to get the work done A leader is a visionary, who must...

Ngày tải lên: 26/12/2014, 08:36

... aggregation Thus, in proteins, only two states are inactive (they are native states) In all other cases they are active, as manifest in the capacity for native aggregation The proposed mechanism ... aggregation) to form new structures that have signaling and functional significance for the active cell Native aggregation is determined by the same forces and interactions that are involved in ... hydrophobic binding centers for dyes are absent; and intracellular water is a poor solvent for them For these reasons, the dye molecules penetrating into the cell are eventually pushed out into the...

Ngày tải lên: 13/08/2014, 16:20

... from all hospitals that contribute data to HCUP For each hospital, 10 percent of normal newborns and 80 percent of all other neonatal and pediatric stays are randomly selected Weights are provided ... intervals for the 2000, 2003 and 2006 estimates are extremely narrow and the difference in rates are statistically significant at the level of alpha = 0.05, the differences in rates are small and may ... weighted estimates derived from HCUP database for numerator data, and information from the US Census 2000, and population estimates for 2003 and 2006 for the denominators The database offers the...

Ngày tải lên: 13/08/2014, 18:22

... recommendations are based on a consensus that all communications services, including interactive, information, and entertainment services, will eventually be provided over broadband At the same ... ethnicity, and disabilities) A small percentage of USF funds should be used for outreach to make more educators, librarians, and rural health care providers aware of the programs and for evaluation ... across all platforms, and can make content available for citizen engagement and reuse Digital Media and Young Audiences The Internet and other new digital technologies hold great promise for youth,...

Ngày tải lên: 15/02/2014, 19:20

... previous month and landing performance and reflects the fact that both recent and cumulative training contribute to improved performance - 18 Table 3.1 Career Training and F-14 Landing Performance ... the overall readiness of the force because junior personnel not receive the type and quantity of training that they need and are sometimes even forced to become trainers before they are ready ... Experience 13 Table 3.1 Career Training and F-14 Landing Performance 18 Table 3.2 Training in Previous Month and F-14 Landing Performance 18 Table 3.3 Career Training Hours and Bombing Error 19...

Ngày tải lên: 17/02/2014, 22:20

... A3-crystallin) and one basic (bB2crystallin) subunit as representatives of all b-crystallins for probing the calcium-binding properties We have selected bB2-crystallin because it is the major crystallin ... crystallin among all b-crystallins and its 3D structure is known [27] bA1- and bA3-crystallins are identical in sequence except for N-terminal extension of 17 amino acids in bA3-crystallin Moreover, ... FEBS 4139 All lens b-crystallins are calcium-binding proteins M K Jobby and Y Sharma Table Binding constants and the enthalpy change of calciumand terbium-binding to bB2- and bA3-crystallins K,...

Ngày tải lên: 18/02/2014, 16:20

... D, H and Q, residue Y70 to W, residue Q81 to N and residue R105 to K and H The kinetic properties of the active site mutants are summarized in Table All mutants were characterized with dThd and ... approximately sixfold and kcat decreased approximately fivefold compared with the wild-type Thus, for Y70W, the changes in Km with these analogues were less pronounced than for dThd and dCyd Overall, changing ... of residues 1–11 and 210–230 The loop connecting a9 and b5 (residues 195–200) was flexible and poor density was observed The electron density for the mutant residue and the ligand was well defined...

Ngày tải lên: 19/02/2014, 02:20

... desensitization [20–23] and patterns of expression [24], this represents a question of potentially immense importance The fact that TPa and TPb are differentially expressed and are under the transcriptional ... receptor gene expression tion and cells lysed and prepared for luciferase assays as described above HEK293 and HEL cell supernatants were assayed for both firefly and renilla luciferase activity ... autoradiography, as outlined (C and D) Western blot analysis of Oct-1 (C) and Oct-2 (D) expression in whole cell protein (60 lgÆ lane)1) prepared from HEL (C and D; lane 1) and HEK293 (C and D; lane 2) cells...

Ngày tải lên: 19/02/2014, 16:20

... generating and redistributing the benefits of the city for all We are aware of the challenges of this particular aspiration for social justice Some call it wishful thinking or an illusion We call it ... accessible to all, environmentallyfriendly and economy-focused.”2 IVM emphasizes “movement and mobility in the city, a right and a pleasure,” explaining that “the means for people, goods, and information ... role for the freedom, independence and quality of life The committee states that they are “aware that the right to mobility is universal to all human beings, and is essential for the effective and...

Ngày tải lên: 25/02/2014, 01:20

... Jeannine, my grandparents, and the rest of my family for their faith and prayers for this project and their understanding when I sometimes nodded off on a couch at family gatherings For every blessing ... Thank you for taking part in this experiment Andy and Dave Design Accessible Web Sites Thirty-Six Keys to Creating Content for All Audiences and Platform Jeremy J Sydik The Pragmatic ... editor, reviewer, cover designer, and first audience for this project More importantly, you are the mother of my son, my girlfriend, my best friend and my wife The things I here and elsewhere are...

Ngày tải lên: 05/03/2014, 11:21

... can be found at http://www.federalreserve.gov/reportforms/formsreview/FRY14Q 20111216 f.pdf and http://www.federalreserve.gov/reportforms/formsreview/FRY14A 20120118 f.pdf.[endoffootnote19.] characteristics, ... 2011.[endoffootnote20.] information) or early January (for forms containing BHC-derived estimates) BHCs were required to submit detailed loan and securities portfolio information for all material portfolios, ... the ALLL are calculated as projected loan losses for the quarter plus or minus the amount needed for the ALLL to be at an appropriate level at the end of the quarter The Federal Reserve's forward-looking...

Ngày tải lên: 06/03/2014, 09:20

... property that has also been observed recently for wildtype and mutant forms of aB-crystallin [60,61] The E190A and E204A mutants performed poorly compared with the wild-type protein in both assays, ... Fig 1) are tallied Only the totals are given for murine sHsps Residues that promote disorder are in bold and those that promote order are in italic [32] Residues are denoted as charged (+ or )), ... The IXI motifs are shown in italics and the known flexible regions of various sHsps, as determined by NMR spectroscopy [26,31,76], are in bold Residues used in the tally for Table are underlined...

Ngày tải lên: 07/03/2014, 04:20

... genes normally coding for eEF2 These cells are viable due to the presence of an URA3-plasmid, pYES2.1, containing the gene coding for wild-type eEF2 (Table 1) The transformed GB1 cells were allowed ... strain For cloning of the yeast eEF2 gene, total yeast DNA was prepared form strain YDR385w as described by Hoffman and Winston [47] The gene for eEF2 was amplified by PCR using primers eEF2F and ... vector pYES2.1 and the resulting plasmid was transformed into strain YOR133w carrying only one of the two genomic alleles for eEF2 The transformed cells were plated onto SC-Ura and a positive...

Ngày tải lên: 07/03/2014, 05:20

... Lys59 (from the N-terminus) is inactive and probably misfolded Materials and methods Expression plasmids All of the GCPII variants used in this study are schematically depicted in Fig Fig Schematic ... fold are cycled via the calnexin-calreticulinglucosidase I and II system within the ER lumen, providing space and time for the unfolded/partially folded proteins to acquire the correct 3D conformation ... cells The expression and carboxypeptidase activities of the individual constructs were analyzed both in cell lysates and conditioned media, and the results are summarized in Fig and Table 2, respectively...

Ngày tải lên: 07/03/2014, 15:20

... pathway, and further, Ybr004cp-depletion gives rise to cell wall and morphological defects characteristic of GPI assembly mutants We therefore propose that Ybr004cp is an excellent candidate for GPI-MT-II ... ethanolamine and mm choline, and metabolic labeling was performed with 20 lCi [14C]ethanolamine for  23 h at 25 °C For radiolabeling of double mutant strains, cells were grown in SGlyYE medium for days ... strain for growth and biochemical defects characteristic of a GPI anchoring deficiency Strains defective in GPI anchoring are typically hypersensitive to the fluorescent dye Calcofluor white (CFW) and...

Ngày tải lên: 07/03/2014, 16:20

... solfataricus The side chains of the putative catalytic residues are shown Ser171 and Lys96, shared by the two triads are in red catalytic triad and forms an angle of about 107° with its plane (Fig 5A) Homology ... thiomidate and acyl-enzyme intermediates, and tetrahedral ( 109.5°) water-bonded intermediate In contrast, serine and thiol protease and amidase are confined to interacting with planar substrates and ... Bstz17I PCR amplification was performed at 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 30 s with 2.5 U of Taq polymerase, in · reaction buffer, MgCl2 1.5 mm for 24 cycles; dNTP were used each...

Ngày tải lên: 07/03/2014, 21:20

... templates TATAWtUTRmut and TATAmutUTRmut) We found that both TBP (Fig 2A, compare lanes and 5) and the R2 TATA-box (Fig 2A, compare lanes and 3) were absolutely required for transcription from ... pairs and Py-Im targets CÆG base pairs and the Py-b-alanine pair recognizes both AÆT and TÆA base pairs [38] The boxed sequence represents the atypical R2 TATA-box Black and white circles, Im and ... composed of two subunits, proteins R1 and R2 Both subunits are required for enzyme activity and it could be reasonable to assume that the expression of the R1 and R2 mRNAs should be coordinated...

Ngày tải lên: 08/03/2014, 02:20