action potential in brain cells
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... of
pro-inflammatory cytokines from gastric epithelial
cells resulting in the increased generation of ROS in
gastric epithelial cells [24]. It has been shown that Hp
infection can increase ... Medicine.
Reagents
F12 culture medium (Hangzhou Jino Biology
Co., Ltd. China), fetal bovine serum (FBS), ampicillin,
penicillin and streptomycin (Shanghai
Bio-engineering Co., Ltd. China), ... smear and biochemical reactions, and
the washed with 5 ml of brain heart infusion liquid
medium. The eluate was incubated with brain heart
infusion liquid medium containing 10% FBS and Hp
selector....
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
Ngày tải lên :
31/10/2012, 15:12
... was examined using
a fluorescent microscope. Fluorescence was mainly
observed in cytosol areas in transfected cells.
FITC-labeled Bag-1 Morpho/AS was transfected into
almost all JAR cells by ...
overexpressed in cytotrophoblasts, and a decrease in
the expression of Bcl-2 and increase of apoptotic cells
are seen in preeclampsia. Thus antiapoptotic proteins
can play an important role in preventing ... shown in Fig. 3, 12hr exposure to
hypoxia caused almost no increase in the phosphory-
lated JNKs protein level in the cell line.
Twenty-four-hour exposure to hypoxia resulted in a
Int. J....
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
Ngày tải lên :
31/10/2012, 15:37
... Kinne RKH. Effects of doxorubicin, mitomycin C,
and ethanol on Hep-G2 cells in vitro. J Cancer Res Clin Oncol
1999;125:1-8.
26. Higashi K, Hoshino M, Nomura T, et al. Interaction of protein ... microgram quantities of protein utilizing the
principle of protein-dye binding. Anal Biochem 1976;72:248-54.
24. Pelech S, Sutter C, Zhang H. Kinetworks protein kinase
multiblot analysis. Methods ... ethanol induces
phosphorylation of protein kinases
Table 1 shows the effect of 1 mM ethanol on
protein kinases phosphorylation in HepG2 cells after
10 min exposure time compared to untreated cells. ...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
Ngày tải lên :
31/10/2012, 16:49
...
Elevated c-Jun expression in acute myeloid leukemias inhibits
C/EBPalpha DNA binding via leucine zipper domain
interaction. Oncogene. 2003;22(30):4760-4.
17. Staber PB, Linkesch W, Zauner D, Beham-Schmid ... acts as an adaptor protein providing substrate
specificity for SCF (Skp–Cullin–F-box) ubiquitin
ligases which are involved in tagging proteins for
degradation in the proteasome.
hCDC4 has been ... obtained at the time of their initial
diagnosis after informed consent. For control, 51 PB
samples were obtained from voluntary healthy
individuals after informed consent. Mononuclear cells...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
Ngày tải lên :
03/11/2012, 11:17
...
involved in claudin-1 expression and localization.
Indeed, a study shows that increasing β-catenin in-
creases the expression of claudin-1 in colorectal cancer
cells, and that claudin-1 expression ... claudin-1.
Nuclear claudin-1 does not increase melanoma
cell motility.
We have previously shown that increasing the
levels of CLDN-1 increases the invasion of melanoma
cells (4). To determine ... claudin-1 in the cytoplasm, with some nuclear expression of claudin-1. B)
Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin-1 in as little as 15 minutes,...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
Ngày tải lên :
03/11/2012, 11:44
... ace-
tonitrile in water (B). The peptide was eluted with a
successive linear gradient of 10% B to 80% B in 30 min
at a flow rate of 23 ml/min. The fractions corre-
sponding to the purified protein were ... cytoplasmic gene
product is a serine/threonine-protein kinase N1
which is a member of the protein kinase C superfam-
ily (http://amigo.geneontology.org/cgi-bin/amigo/
gp-details.cgi?gp=UniProtKB:Q16512). ... Software and are
described in the following part according the defini-
tions of the Illumina’s (ILL) data as well as of the Il-
lumina’s annotations (table 2).
Int. J. Med. Sci. 2009, 6
...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
Ngày tải lên :
14/02/2014, 22:20
... carcinoma cells were grown in
DMEM containing 10% heat-inactivated fetal bovine
serum, 2 mm glutamine, 100 UặmL
)1
penicillin, and
100 ngặmL
)1
streptomycin, at 37 C in air with 5% CO
2
.
Cells ... Pharmigen, San Diego, CA, USA) binding and
PI staining were performed according to [44]. Following
incubation with 1.0 lgặmL
)1
annexin V and 4.0 lgặmL
)1
PI
for 20 min, cells were analyzed by flow ... (SFAs) led to increased expression of proin-
flammatory cytokines, inhibition of insulin signaling,
induction of endoplasmic reticulum (ER) stress, and
promotion of cell death, mainly by apoptosis...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
Ngày tải lên :
16/02/2014, 09:20
... the length of linkers. In the linker con-
taining seven amino acids, a Pro-Pro sequence that is
not contained in other linkers is incorporated. This less
flexible structure may inhibit fluorophores ... linked to
cerulean or citrine were introduced into CHO-K1 cells.
As shown in Figs 1A and S1A, transiently expressed
GAPDH–citrine (chimeric protein with N-terminal
GAPDH and C-terminal citrine) ... relative ori-
entation of two fluorophores, being independent of the
strength of protein–protein interactions. In the case of
very weak interactions as described in this article,
rapid association and...
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Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf
Ngày tải lên :
18/02/2014, 11:20
... thought by providing
just such an interdisciplinary forum, in which research
results, including novel concepts such as environmen-
tally induced increases in mutation rates in bacteria
and a ... gene regulons
and regulatory pathways involved in freezing tolerance
in Arabidopsis. Freezing tolerance increases in many
plants in response to low, non-freezing temperature, a
process known as ... exposure
depending on the time of day of the exposure [51].
Cold induction of CBF2 involves multiple cis-acting
regulatory elements, one of which binds members of
the calmodulin-binding transcription...
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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
Ngày tải lên :
18/02/2014, 11:20
... determine the viability of the cells, trypsinized cells were
suspended in serum-containing medium. In order to remove
serum, cells were washed three times with serum-free med-
ium prior to seeding ... osteosarcoma cells in our study where
the cells appeared more rounded in shape. In human
skin fibroblasts there is an increase in proMMP-2 acti-
vation that is independent of the lattice contraction
[23]. ... MMPs are secreted as inactive proenzymes and
latency is maintained by an interaction between a
cystein residue in the prodomain and Zn
2+
in the
active site of the catalytic domain. Two major types...
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Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc
Ngày tải lên :
18/02/2014, 12:20
... specific peptide-
binding domain found in poly(A)-binding protein and a
HECT ubiquitin ligase. EMBO J 23, 272–281.
9 Craig A (1998) Interaction of polyadenylate-binding
protein with the elF4G ... fractions. In
contrast, almost 90–100% of the b-actin mRNA was
present in the polysomal fractions. There was a signifi-
cant reduction in the translation of the b-actin mRNA
in heat-shocked cells, ... some
circumstances [32,33]. A small fraction of eIF4G has
been previously shown to be present in the nuclear
fraction, and to bind the cap-binding complex involved
in splicing [34]. It is believed that...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
Ngày tải lên :
18/02/2014, 18:20
... to an increase in ICAM-1 expression in Schwann
cells of sciatic nerves
Aiguo Shen
1,
*, Junling Yang
2,
*, Yangyang Gu
3
, Dan Zhou
4
, Linlin Sun
2
, Yongwei Qin
2
,
Jianping Chen
2
, Ping Wang
2
, ... Schwann
cells) and CD31 (a marker of endothelial cells) . In pre-
vious studies, ICAM-1–integrin interactions mediated
adhesion of leukocytes to the vascular endothelium,
revealing a key role in migration ... of cytokine
induced expression of intercellular adhesion molecule 1
(ICAM-1) in an intestinal epithelial cell line, a process
blocked by proteasome inhibitors. Gut 42, 779–787.
23 Lakshminarayanan...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
Ngày tải lên :
19/02/2014, 00:20
... protein-induced
T. U. Wagner BMP signaling in stem cells
FEBS Journal 274 (2007) 29682976 ê 2007 The Author Journal compilation ª 2007 FEBS 2975
MINIREVIEW
Bone morphogenetic protein signaling in ... neuroepithelial
cells into astrocytes, by binding and hyper activating
the promoter of glial fibrillary acidic protein (GFAP).
In parallel, BMP signals lead to enhanced expression
of ID proteins, which in ... triggering.
There is another example of stemness decisions by
Nodal versus BMP signaling emerging from human
embryonic stem cell research. Testing human ES -cells
BMP signaling in stem cells T....
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Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf
Ngày tải lên :
19/02/2014, 12:20
... in Dulbeccos modied Eagles medium
containing 10% foetal bovine serum, 100 UặmL
)1
penicillin,
1.0 mgặmL
)1
streptomycin and 2 m
ML
-Glu. For treating
BxPC3 cells and clones with drugs affecting ... (under-
lined), and a 27-base sequence corresponding to nucleotide
334–360 in the coding region of the gene. PCR reactions
were incubated as follows: 94 °C for 3.5 min followed by 25
cycles of 1.5 min ... by staining with ethidium bromide. (B)
b1,3Gal-T activity in BxPC3 cells (j)orinT5ASclone(h)was
determined with GlcNAc as acceptor using different amounts of cell
homogenates for a fixed incubation...
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