action potential in brain cells
Báo cáo y học: "New role for Agrin in T cells and its potential importance in immune system regulation" ppsx
Ngày tải lên: 12/08/2014, 12:20
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... of pro-inflammatory cytokines from gastric epithelial cells resulting in the increased generation of ROS in gastric epithelial cells [24]. It has been shown that Hp infection can increase ... Medicine. Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd. China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd. China), ... smear and biochemical reactions, and the washed with 5 ml of brain heart infusion liquid medium. The eluate was incubated with brain heart infusion liquid medium containing 10% FBS and Hp selector....
Ngày tải lên: 25/10/2012, 11:18
Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... was examined using a fluorescent microscope. Fluorescence was mainly observed in cytosol areas in transfected cells. FITC-labeled Bag-1 Morpho/AS was transfected into almost all JAR cells by ... overexpressed in cytotrophoblasts, and a decrease in the expression of Bcl-2 and increase of apoptotic cells are seen in preeclampsia. Thus antiapoptotic proteins can play an important role in preventing ... shown in Fig. 3, 12hr exposure to hypoxia caused almost no increase in the phosphory- lated JNKs protein level in the cell line. Twenty-four-hour exposure to hypoxia resulted in a Int. J....
Ngày tải lên: 31/10/2012, 15:12
Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
... Kinne RKH. Effects of doxorubicin, mitomycin C, and ethanol on Hep-G2 cells in vitro. J Cancer Res Clin Oncol 1999;125:1-8. 26. Higashi K, Hoshino M, Nomura T, et al. Interaction of protein ... microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54. 24. Pelech S, Sutter C, Zhang H. Kinetworks protein kinase multiblot analysis. Methods ... ethanol induces phosphorylation of protein kinases Table 1 shows the effect of 1 mM ethanol on protein kinases phosphorylation in HepG2 cells after 10 min exposure time compared to untreated cells. ...
Ngày tải lên: 31/10/2012, 15:37
Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... Elevated c-Jun expression in acute myeloid leukemias inhibits C/EBPalpha DNA binding via leucine zipper domain interaction. Oncogene. 2003;22(30):4760-4. 17. Staber PB, Linkesch W, Zauner D, Beham-Schmid ... acts as an adaptor protein providing substrate specificity for SCF (Skp–Cullin–F-box) ubiquitin ligases which are involved in tagging proteins for degradation in the proteasome. hCDC4 has been ... obtained at the time of their initial diagnosis after informed consent. For control, 51 PB samples were obtained from voluntary healthy individuals after informed consent. Mononuclear cells...
Ngày tải lên: 31/10/2012, 16:49
Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... involved in claudin-1 expression and localization. Indeed, a study shows that increasing β-catenin in- creases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 expression ... claudin-1. Nuclear claudin-1 does not increase melanoma cell motility. We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4). To determine ... claudin-1 in the cytoplasm, with some nuclear expression of claudin-1. B) Treatment of these cells with PKA inhibitor results in nuclear translocation of claudin-1 in as little as 15 minutes,...
Ngày tải lên: 03/11/2012, 11:17
Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... ace- tonitrile in water (B). The peptide was eluted with a successive linear gradient of 10% B to 80% B in 30 min at a flow rate of 23 ml/min. The fractions corre- sponding to the purified protein were ... cytoplasmic gene product is a serine/threonine-protein kinase N1 which is a member of the protein kinase C superfam- ily (http://amigo.geneontology.org/cgi-bin/amigo/ gp-details.cgi?gp=UniProtKB:Q16512). ... Software and are described in the following part according the defini- tions of the Illumina’s (ILL) data as well as of the Il- lumina’s annotations (table 2). Int. J. Med. Sci. 2009, 6 ...
Ngày tải lên: 03/11/2012, 11:44
Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... carcinoma cells were grown in DMEM containing 10% heat-inactivated fetal bovine serum, 2 mm glutamine, 100 UặmL )1 penicillin, and 100 ngặmL )1 streptomycin, at 37 C in air with 5% CO 2 . Cells ... Pharmigen, San Diego, CA, USA) binding and PI staining were performed according to [44]. Following incubation with 1.0 lgặmL )1 annexin V and 4.0 lgặmL )1 PI for 20 min, cells were analyzed by flow ... (SFAs) led to increased expression of proin- flammatory cytokines, inhibition of insulin signaling, induction of endoplasmic reticulum (ER) stress, and promotion of cell death, mainly by apoptosis...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... the length of linkers. In the linker con- taining seven amino acids, a Pro-Pro sequence that is not contained in other linkers is incorporated. This less flexible structure may inhibit fluorophores ... linked to cerulean or citrine were introduced into CHO-K1 cells. As shown in Figs 1A and S1A, transiently expressed GAPDH–citrine (chimeric protein with N-terminal GAPDH and C-terminal citrine) ... relative ori- entation of two fluorophores, being independent of the strength of protein–protein interactions. In the case of very weak interactions as described in this article, rapid association and...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf
... thought by providing just such an interdisciplinary forum, in which research results, including novel concepts such as environmen- tally induced increases in mutation rates in bacteria and a ... gene regulons and regulatory pathways involved in freezing tolerance in Arabidopsis. Freezing tolerance increases in many plants in response to low, non-freezing temperature, a process known as ... exposure depending on the time of day of the exposure [51]. Cold induction of CBF2 involves multiple cis-acting regulatory elements, one of which binds members of the calmodulin-binding transcription...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
... determine the viability of the cells, trypsinized cells were suspended in serum-containing medium. In order to remove serum, cells were washed three times with serum-free med- ium prior to seeding ... osteosarcoma cells in our study where the cells appeared more rounded in shape. In human skin fibroblasts there is an increase in proMMP-2 acti- vation that is independent of the lattice contraction [23]. ... MMPs are secreted as inactive proenzymes and latency is maintained by an interaction between a cystein residue in the prodomain and Zn 2+ in the active site of the catalytic domain. Two major types...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc
... specific peptide- binding domain found in poly(A)-binding protein and a HECT ubiquitin ligase. EMBO J 23, 272–281. 9 Craig A (1998) Interaction of polyadenylate-binding protein with the elF4G ... fractions. In contrast, almost 90–100% of the b-actin mRNA was present in the polysomal fractions. There was a signifi- cant reduction in the translation of the b-actin mRNA in heat-shocked cells, ... some circumstances [32,33]. A small fraction of eIF4G has been previously shown to be present in the nuclear fraction, and to bind the cap-binding complex involved in splicing [34]. It is believed that...
Ngày tải lên: 18/02/2014, 12:20
Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... to an increase in ICAM-1 expression in Schwann cells of sciatic nerves Aiguo Shen 1, *, Junling Yang 2, *, Yangyang Gu 3 , Dan Zhou 4 , Linlin Sun 2 , Yongwei Qin 2 , Jianping Chen 2 , Ping Wang 2 , ... Schwann cells) and CD31 (a marker of endothelial cells) . In pre- vious studies, ICAM-1–integrin interactions mediated adhesion of leukocytes to the vascular endothelium, revealing a key role in migration ... of cytokine induced expression of intercellular adhesion molecule 1 (ICAM-1) in an intestinal epithelial cell line, a process blocked by proteasome inhibitors. Gut 42, 779–787. 23 Lakshminarayanan...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
... protein-induced T. U. Wagner BMP signaling in stem cells FEBS Journal 274 (2007) 29682976 ê 2007 The Author Journal compilation ª 2007 FEBS 2975 MINIREVIEW Bone morphogenetic protein signaling in ... neuroepithelial cells into astrocytes, by binding and hyper activating the promoter of glial fibrillary acidic protein (GFAP). In parallel, BMP signals lead to enhanced expression of ID proteins, which in ... triggering. There is another example of stemness decisions by Nodal versus BMP signaling emerging from human embryonic stem cell research. Testing human ES -cells BMP signaling in stem cells T....
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf
... in Dulbeccos modied Eagles medium containing 10% foetal bovine serum, 100 UặmL )1 penicillin, 1.0 mgặmL )1 streptomycin and 2 m ML -Glu. For treating BxPC3 cells and clones with drugs affecting ... (under- lined), and a 27-base sequence corresponding to nucleotide 334–360 in the coding region of the gene. PCR reactions were incubated as follows: 94 °C for 3.5 min followed by 25 cycles of 1.5 min ... by staining with ethidium bromide. (B) b1,3Gal-T activity in BxPC3 cells (j)orinT5ASclone(h)was determined with GlcNAc as acceptor using different amounts of cell homogenates for a fixed incubation...
Ngày tải lên: 19/02/2014, 12:20