aberrant b cell expression of class ii mhc

... murine B- cell expression of TLR7 as well as in the IFN-I-mediated induction of TLR9 expression [32] We examined the mRNA expression levels of these TLRs in B cells from IFNAR2-/- mice IFNAR2- /B cells ... not for citation purposes) Because IFN-α upregulated B- cell expression of TLR7 and TLR9, we examined the ability of IFN-α to enhance B- cell activation by TLR ligands B cells from WT mice pretreated ... on IFNAR2 (Figure 4b) IFNAR2 is therefore required for the induction of TLR7 and TLR9 expression in B cells in response to IFN-α and for normal basal levels of B- cell TLR7 expression Toll-like...

Ngày tải lên: 09/08/2014, 14:22

... substrate, type of metal present, and XI class With glucose as the substrate, class I enzymes are best activated by Mg2+, followed by Co2+ and Mn2+, while class II enzymes are best activated by ... enzyme class [26] With glucose as the substrate, class I enzymes are best activated by Mg2+ [38], or, in some cases, by a combination of Mg2+ and Co2+ [11,39] While Co2+ best activates the class II ... labile amino acids, and shortened loops [24] Similar comparisons among class II XIs have not been reported Class II XIs have not been studied to the same extent as class I enzymes, probably because...

Ngày tải lên: 23/03/2014, 13:20

... DEK-binding site, class II MHC Y-box motifs (DQA1, DRA, DQB, and DRB), and related sequences Probes and competitors include only sequences 3′ of the ᭹ symbol X boxes are shown to provide a broader ... the binding reaction before addition of the radiolabeled DQA1*0101 probe (a) or the radiolabeled DRA probe (b) to define the sequence specificity of nuclear protein binding In both (a) and (b) , ... high autoantibody production [26,27] In our proposed model, aberrant < /b> class II MHC regulation could result from altered DEK binding and/or interaction with NF-Y, and aberrant < /b> class II expression...

Ngày tải lên: 09/08/2014, 01:23

... hepatitis B virus surface antigen (HBsAg), hepatitis B virus core antibody (HBcAb) and hepatitis C virus (HCV) antibody Also, tumor markers including carcinoembryonic antigen and carbohydrate ... (L3-AFP), protein induced by vitamin-K absence or antagonist II (desgamma carboxy prothrombin, PIVKA -II) , and soluble interleukin (s-IL2) receptor were within normal limits Abdominal ultrasonography ... needle biopsy, non-resection treatment procedures would be permitted, such as radiofrequency ablation [37] instead of surgical resection Furthermore, in case of HPL, simple observation proved to be...

Ngày tải lên: 09/08/2014, 01:24

... ventricle Surgical removal was laborious but without complications The tumor was submitted to the pathology laboratory as white and red soft fragments measuring cm by cm by cm (Figures and 6) Histological ... non-Hodgkin large B- cell lymphoma (CD45+ CD20+ CD3- BCl2+ CD20- CD10- BCl6-) The Figure Computed tomography scan showing the presence of a right heart tumor developing on both sides of the tricuspid ... images A copy of the written consent is available for review by the Editor-in-Chief of this journal Acknowledgements We thank Pr Mourad Hentati for his collaboration to elaborate this observation...

Ngày tải lên: 10/08/2014, 23:20

... the increasing expression of KLF4 in PBMC and THP-1 cells, and identified that induction of KLF4 by HDL3 promoted the expression of hSR-BI It has been shown that disruption of SR-BI in mice impairs ... immunoreactive bands were visualized using diaminobenzidine (DAB) (Boster Biological Technology, Wuhan, Hubei, China) The following antibodies were used: rabbit SRBI polyclonal antibody (1 : 1000, Abcam, ... real-time PCR (B) Protein levels of KLF4 were determined by western blot (C) mRNA levels of hSR-BI were determined by real-time PCR (D) Protein levels of hSR-BI were determined by western blot The...

Ngày tải lên: 18/02/2014, 04:20

... Galb1-4GlcNAcb-O-octyl Galb1-3GlcNAcb-O-octyl Galb1-3GalNAca1-O-bn Galb1-4GlcNAc Galb1-3GlcNAc LNnT: Galb1-4GlcNAcb1-3Galb1-4Glc LNT: Galb1-3GlcNAcb1-3Galb1-4Glc 100 (0.52 )b 0 100 (13.86 )b 1.4 ... Biochem 270) rST3Gal III gene was excised with BamHI and HindIII and inserted at the BglII–HindIII site of the p119 transfer vector giving the p119-PS ST3Gal III construct Sf9 cells (ATCC CRL1711) ... Construction of recombinant baculoviruses and production of soluble ST6Gal I and ST3Gal III in Sf9 In order to express soluble and His6-tagged enzymatic forms of human ST6Gal I (GenBank accession number...

Ngày tải lên: 08/03/2014, 08:20

... Several non-classical MHC class I, Qa1a-restricted CD8aa+TCRab+ T cell and MHC class II- restricted CD4+ T cell clones and lines have been characterized [8,9,11] The CD4+ T cells recognize a TCR-derived ... the context of a class II MHC molecule, I-Au [11] However, CD8aa+TCRab+ T cells are cytotoxic and recognize another TCR-derived peptide bound to a class Ib molecule, Qa-1 [8,9] These cells are ... loading of class I-restricted, clinically relevant TAA-derived peptides to human DC, and the development of nanoparticles incorporating MHC class II and non-classical MHC class I, Qa-1-binding...

Ngày tải lên: 18/06/2014, 19:20

... Several non-classical MHC class I, Qa1a-restricted CD8aa+TCRab+ T cell and MHC class II- restricted CD4+ T cell clones and lines have been characterized [8,9,11] The CD4+ T cells recognize a TCR-derived ... the context of a class II MHC molecule, I-Au [11] However, CD8aa+TCRab+ T cells are cytotoxic and recognize another TCR-derived peptide bound to a class Ib molecule, Qa-1 [8,9] These cells are ... loading of class I-restricted, clinically relevant TAA-derived peptides to human DC, and the development of nanoparticles incorporating MHC class II and non-classical MHC class I, Qa-1-binding...

Ngày tải lên: 20/06/2014, 03:20

... Memory B cells 60% Memory B cells 60% BBcells cells B cells CD27 Expression CD27 Expression Plasmablasts Naïve Naïve B cells B cells Memory Memory B cells B cells CD27 Expression Schematic distribution ... memory B cells and a normal frequency of CD27– naive B cells The pattern of B cell subpopulations in pSS, RA and SLE defined by CD27 expression therefore seemed to be unique The difference between ... and CD70 expressed by B cells only [77] was also reported to be sufficient for B cell differentiation, raising the possibility that B cells might be able to regulate themselves by CD27–CD70 interactions...

Ngày tải lên: 09/08/2014, 01:21

... -4 -3 -2 -1 Binding score distribution 0.1 >6 Binding score distribution DR4 binding score analysis of the endogenous antigens (a) human type II collagen (hCII), the (b) first globular domain ... peptides that are capable of activating DR4 restricted T cells, it must be noted that additional factors influence the availability of peptides for presentation by MHC class II on APCs Included ... its ability to activate IFN-γ secreting T cells from DR4-IE transgenic mice We also showed that hAG and HBsAg may be more immunogenic in the context of DR4 than CII based on the number of predicted...

Ngày tải lên: 09/08/2014, 01:21

... in a bacterial expression system [1,2] Crawford et al [6] described the use of MHC class II molecules with covalently attached peptides produced in a baculovirus expression system MHC class II ... necessary for MHC class II/ peptide multimer production [10,11] Other expression systems have been used to generate MHC class II/ peptide multimers Boniface et al [11] produced MHC class II molecules ... technical description Production of multimeric MHC class II/ peptide staining reagents involves four basic steps: the expression of soluble monomeric MHC class II molecules, peptide loading, oligomerization,...

Ngày tải lên: 09/08/2014, 03:24

... in TBS (pH7.6), the specimens were incubated for h at 37°C with anti-STAT3 antibody (sc-7179 rabbit polyclonal antibody, Santa Cruz Biotechnology) for STAT3, anti-P-STAT3 antibody (9145, rabbit ... two subgroups, the CHOP subgroup and the R-CHOP subgroup In CHOP subgroup, high nuclear expression of STAT3 predicted poor survival (P = 0.001) In RCHOP subgroup, of 19 cases died of DLBCL in ... Varin-Blank N, Ajchenbaum-Cymbalista F, Ledoux D: Constitutive and B- cell receptor-induced activation of STAT3 are important signaling pathways targeted by bortezomib in leukemic mantle cell lymphoma...

Ngày tải lên: 10/08/2014, 21:23

... A549 cell permeability is significantly attenuated by the knockdown of PBEF expression by PBEF siRNA Overexpression of PBEF increased A549 cell permeability in both basal and TNFα Page 12 of 15 ... Figure A549 of PBEF expression on in vitro cell permeability of Effectscells and HPAEC Effects of PBEF expression on in vitro cell permeability of A549 cells and HPAEC A549 cells or HPAEC cells were ... described in the Methods A&C Effects of PBEF underexpression on in vitro cell permeability of A549 cells and HPAEC; B& D Effects of PBEF overexpression on in vitro cell permeability of A549 cells...

Ngày tải lên: 11/08/2014, 08:22

... sites of pMX The BglII site of pMX kBL1 was destroyed by cutting by BglII, filling in by T4 polymerase, and self-ligation to make pMX kBL1∆BglII Primary ATL cells or T -cell lines were incubated ... stable expression of CD30 induces constitutive NF- B activation in Rat-1 cells Exogenous stable expression of CD30 induces constitutive NF- B activation in Rat-1 cells Rat-1 B- bsrEGFPx2 cells ... by inserting a MluI – NotI fragment of pCD30WT [64] in the EcoRI and NotI sites of pMX by blunt-end ligation pMX kBL1 was generated by inserting a BamHI – SfiI fragment of pGEMT kBL1 in the BamHI...

Ngày tải lên: 13/08/2014, 09:21

... stimulation of HBV viral proteins indeed changes the expression of apoptosis profile of host cells, which probably results in the carcinogenesis of liver cells Materials and methods Cell lines ... membrane (BioRad, Hercules CA) and images were analyzed by Phosphorimager Results The profile of IAPs expression in the cells producing HBV The change of IAPs expression in the HBV expressing cells ... increase of expression of apoptosis inhibitor is thought to be a major cause of the tumor formation/progression Through the inhibition of cell apoptosis, cancer cells acquire the ability of unlimited...

Ngày tải lên: 02/11/2012, 11:17

... analyzed the expression profiles of HO-1 and HO-2 in eight human cell lines by northern and western blot analyses (Fig 1A ,B) Expression of HO-1 5334 A B C Fig Expression profiles of HO-1 and HO-2 ... erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung cancer cells, and HMV -II melanoma cells H146 small cell lung cancer cells were obtained from ATCC (HTB-173) and ... correlation between the expression levels of HO-1 and HO-2 and cellular heme content The cellular heme content represents the sum of free heme and bound heme of various hemoproteins for maintenance of cellular...

Ngày tải lên: 19/02/2014, 05:20

... Effects of hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme ... oxygenase-2 A B Fig Effects of hypoxia on expression of heme oxygenase (HO)-1 and HO-2 in human cell lines of bone marrow origin (A) Northern blot analysis of HO-1 and HO-2 mRNA KG1 myeloid cells, Jurkat ... in KG1 cells Under hypoxia, expression levels of b- actin mRNA were unchanged We next measured the levels of HO-1 and HO-2 proteins by western blot analysis (Fig 1B) The expression levels of HO-1...

Ngày tải lên: 19/02/2014, 06:20

... adhesion inhibition (Eur J Biochem 271) 2877 Fig Ribbon diagram of crystal structure of CD2–CD58 complex and crystal structure of rat CD2 (A) Ribbon diagram of crystal structure of CD2–CD58 (LFA-3) ... surface The ability of Jurkat cells to express CD2 was 2878 L Jining et al (Eur J Biochem 271) Fig Inhibition or enhancement of FITC-labeled CD58-antibody binding to MOLT-3 cells by synthetic ... formation of E-rosettes The ability of each of the designed CD2 peptides to inhibit CD2–CD58 interaction was evaluated by inhibition of E-rosette formation between Jurkat cells and SRBCs As depicted...

Ngày tải lên: 19/02/2014, 13:20

... (subG0 ⁄ G1), but with an increase of cells in G1 phase, i.e an increase in cell arrest (Fig 3B) Expression of immunoregulatory molecules by HepG2 cells Next, we examined the expression of cell ... mechanism for T cell activation by foam cells Am J Pathol 155, 775–786 Bonish B, Jullien D, Dutronc Y, Huang BB, Modlin R, Spada FM, Porcelli SA & Nickoloff BJ (2000) Overexpression of CD1d by keratinocytes ... loading of HepG2 cells, a hepatocytic cell line, resulted in increased cell surface expression of CD1d molecules recognized by Nor3.2 but not by CD1d42 antibodies Interestingly, CD1d42 antibodies...

Ngày tải lên: 19/02/2014, 16:20