a systematic review and meta analysis

... read and approved the final manuscript Material and Methods Virus culture and virus detection by real-time PCR Camelpox strain CP-19, CMV strain AD169, HHV-6 strain U1102, SARS coronavirus strain ... Acknowledgements We gratefully acknowledge the excellent technical assistance of Delia Barz and Jung-Won Sim-Bandenburg The authors are grateful to Andreas Kurth for critical reading of the manuscript References ... asthmatic airways is variable and not suitable for normalising mRNA levels Thorax 2002, 57:765-770 Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, Griffiths LR: Beta-actin an unsuitable...

Ngày tải lên: 18/06/2014, 22:20

... read and approved the final manuscript Material and Methods Virus culture and virus detection by real-time PCR Camelpox strain CP-19, CMV strain AD169, HHV-6 strain U1102, SARS coronavirus strain ... Acknowledgements We gratefully acknowledge the excellent technical assistance of Delia Barz and Jung-Won Sim-Bandenburg The authors are grateful to Andreas Kurth for critical reading of the manuscript References ... asthmatic airways is variable and not suitable for normalising mRNA levels Thorax 2002, 57:765-770 Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, Griffiths LR: Beta-actin an unsuitable...

Ngày tải lên: 20/06/2014, 04:20

... Pharmacol 2000, 58:145-151 Shankavaram UT, Lai WC, Netzel-Arnett S, Mangan PR, Ardans JA, Caterina N, et al.: Monocyte membrane type 1-matrix metalloproteinase Prostaglandin-dependent regulation ... following activation with 1.5 mM p-aminophenyl mercuric acetate (APMA) Statistical analysis Data were expressed as mean ± SEM or 95% confidence interval (95% CI) and analysed using GraphPad Prism ... stimulation with calcium and calmodulin [34] and it is now recognized that the cAMP-protein kinase A pathway regulates the expression [38] and catalytic activity of PDE4 variants [10,13] as well as...

Ngày tải lên: 12/08/2014, 16:20

... TGACAGGGACAACGACCCTCTCCCAGGGGCGACAGCAAGCCCCCAAGGACAAAA c-Myb CTAGCAGGGACTAGTCATCAGCCAAAAAGGTCAACTGTCTCACACAAATAAGGAT bZIP910 Distal 21R in PTLV-1/2 CCGAAGGTTCTGACGTCCCAGCCCAGCCTCAAAACCAGGAAATCCATAGAAATGC ... CCGAAGGTTCTGACGTCCCAGCCCAGCCTCAAAACCAGGAAATCCATAGAAATGC Middle 21R ACCTCGCCCTTACCCACTTCCCCTATCATGAAAAACAAAGGCTGTGACGACTACCC Proximal 21R CCTTCCCCAAAAAATTTGCTTAAACCATCAATAAAGACAGCCTAGCCTATATAAGC TATA box poly (A) signal U3 R ATGAGGATGGTTCAGGAGGGGGCTCGCTCTCTTGCCGATCGCCCTGCTCACCTCGA ... the env-tax fragment are 1863EF1 5'CCTGCC AAAACCT GATCACC TATTC3' and 1863TR1 5'CGACAAC TCGTCCATC GATGG3' and 1863EF2 5'CCCTGTATCTCTTCCCACACTGGGTA C3' and 1863TR2 5'GGG GAGCATAATC CACCGGAGATGG3',...

Ngày tải lên: 13/08/2014, 05:21

... climate  Complementary  species  Start of active  period  Number of  generations  per year  French  2.5 cm  cool  Spanish  late spring  1 ­ 2  Spanish  1.25 cm  9    10    11    South African  ... Sample task type 4  Question 9 – 13  Complete the table below.  Choose NO MORE THAN THREE WORDS from the passage for each answer.  Write your answers in boxes 9­13 on your answer sheet.  ... 2.5 cm  cool  Spanish  late spring  1 ­ 2  Spanish  1.25 cm  9    10    11    South African  ball roller  12    13 ……… ...

Ngày tải lên: 04/10/2012, 09:39

... Education shall be free, at least in the elementary and fundamental stages Elementary education shall be compulsory Technical and professional education shall be made generally available and ... financial assistance in case of need; (c) Make higher education accessible to all on the basis of capacity by every appropriate means; (d) Make educational and vocational information and guidance ... from all forms of sexual exploitation and sexual abuse For these purposes, States Parties shall in particular take all appropriate national, bilateral and multilateral measures to prevent: (a) ...

Ngày tải lên: 07/11/2012, 14:17

... internally and externally Learning both at individual and at organizational levels will also have to be of a high order in order to facilitate the necessary continuous improvement and innovation ... strategic change emphasize the importance of having an understanding of the organization climate and culture, and the current attitude of employees and management in order to assess the best way ... play in the development of organizational learning The moves we have outlined above towards a new balance between innovation, trust, and learning on the one hand and new forms of discipline and...

Ngày tải lên: 21/01/2014, 22:20

... CATAGTGGAAAGAGGGATATAAATTATCGCATAAAACAATAAACAAAAAGAAAAATG AGGGAACAAAAGCTGGAG GGGAGTCAGCTGTTCGGTAACAGCATCTTGCATAGGCACATCTAAATCTGTATCCTGTAGGGCGAATTGGG GCAAAGTAAAACAGCACGAAAAAAGTGATTACAAATTTCAAGGGAGATATGATGAGGGAACAAAGGCTGGAG ... GGCAATTGGAGTGACATAGCAGCTACTACAACTACAAAAGCAAAATCTCCACAAAGTAAT CGGATCCCCGGGTTAATTAA CCAAGTGCTTCAATCCTAGAGAAGAAGAAAGGTAAGAAAAAGAAAGGAAAGCAACTTAAT GAATTCGAGCTCGTTTAAAC pFA 6a- HIS3MX6 pFA 6a- HIS3MX6 pFA 6a- 13Myc-HIS3MX6 ... GTAACTTCAGGTAGTAACTGGGCCTTGTATAGCCTTTCTAAACATTCGTCCAACTGTAGGGCGAATTGGG GAAATACTATTGAAGCTCAAAAACATCCATAATAAAAGGAACAATAACAATGGTAAGGGAACAAAAGCTGGAG GCGCCTGGCATTTCTTTATTGTTTCAAGCCATTCGTCGGGGCCTCTAGACTGTAGGGCGAATTGGG GGCAATTGGAGTGACATAGCAGCTACTACAACTACAAAAGCAAAATCTCCACAAAGTAAT...

Ngày tải lên: 07/03/2014, 12:20

... and N130D variants of human arginase I using an antibody raised against E coli agmatinase (AUH) The relative molecular masses of the protein markers (ST), ovoalbumin (42 700) and carboanhydrase ... inhibitions Also as expected for reactions catalyzed by the same molecular entity, the thermal inactivations of arginase and agmatinase activities of the N130D variant were totally coincident (Fig 3A) Any ... The Authors Journal compilation ª 2006 FEBS ´ R Alarcon et al Agmatinase activity of the N130D mutant arginase I Fig Inhibition of wild-type and N130D variants of human arginase I by agmatine (A) ...

Ngày tải lên: 16/03/2014, 12:20

... (BX-9000; KEYENCE, Osaka, Japan) DNA helicase and ATPase activities of the DNA helicases A human MCM4/6/7 complex was prepared, and its DNA helicase activity was measured, as reported previously, ... has in vitro helicase activity Mol Cell 31, 287–293 20 Chino M, Nishikawa K, Tsuchida T, Sawa R, Nakamura H, Nakamura KT, Muraoka Y, Ikeda D, Naganawa H, Sawa T et al (1997) Heliquinomycin, a ... layer chromatography The radioactivity at the sites to which Pi and ATP migrated was measured, and the ratio of the released Pi to ATP was calculated The ratio obtained in the case of the reaction...

Ngày tải lên: 23/03/2014, 04:21

... Quimby BB, Alano A, Almashanu S, DeSandro AM, Cowan TM & Fridovich-Keil JL (1997) Characterization of two mutations associated with epimerase-deficiency galactosemia, by use of a yeast expression ... have been compared Disease-causing mutations in human GALE Results Expression and purification of human GALE Human GALE was expressed as an N-terminal hexahistidine fusion protein in E coli and ... epimerase-deficiency galactosaemia J Inherit Metab Dis 21, 341–350 Maceratesi P, Daude N, Dallapiccola B, Novelli G, Allen R, Okano Y & Reichardt J (1998) Human UDPgalactose 4¢ epimerase (GALE)...

Ngày tải lên: 23/03/2014, 11:20

... MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped to draft ... been estimated by quantifications of metabolically labelled viral and cellular proteins in the starting material and final preparation [20-22] To estimate these factors for the produced and purified ... centrifugation in a table top Eppendorf centrifuge DNA extraction and quantitative real-time TaqMan PCR Extraction of DNA and determination of viral DNA copies for HHV- 6A was performed as previously...

Ngày tải lên: 18/06/2014, 18:20

... U16-17R gac act cca cca tag atc act c cat gat gca cgc tct acg aga c ctg tga gga act act gtc ttc acg cag cac tcg caa cca ccc tat cag cca gcn cac aaa ggn ata gga gg acb acy gcn cct tch cct ttc ccc aat ... cells are liver macrophages and Hepatocytes are liver endothelial cells 2B-cells priate primer set (Table 2) A band of about 400 bp was seen after PCR analysis, indicating HHV- 6A was replicating ... presence of HIV1 and HCV in the same cell may greatly aggravate the malady Our TEM analysis showed HHV- 6A and HCV in the same cells, with extracellular HIV-1 particles budding or adjacent to the...

Ngày tải lên: 18/06/2014, 18:20

... of C14 6A, C15 7A, C16 1A, C18 5A, C22 5A, C22 9A 5'-AACCTGGCCAGCAGCAACTACTTCCC-3' and 5'-AACTAGAAGGCACAGTCGAGGCTG-3' were used similarly to produce C32 4A, C34 5A, C37 9A The resultant DNA fragments ... C18 5A) , 5'-GTGCCCCTGGACATCGCCACCAGCATC TGCAAG-3' (F for C22 5A) , 5'-CTTGCAGATGCTGGTGGC GATGTCCAGGGGCAC-3' (R for C22 5A) , 5'-ACCAGCATCGCCAAGTACCCCGACTACATC-3' (F for C22 9A) , 5'-ATGTAGTCGGGGTACTTGGCGATGCTGGTGCAGAT ... 5'-CGACAACAGGGAGGCCATCAGCATGGACTACAAG3' (F for C14 6A) , 5'-GTAGTCCATGCTGATGGCCTCCCTGTTGTCCAC-3' (R for C14 6A) , 5'-CAAGCAGACCCAGCTGGCCCTGATCGGCTGCAAG-3' (F for C15 7A) , 5'-CTTGCAGCCGATCAGGGCCAGCTGGGTCTGCTTG-3'...

Ngày tải lên: 18/06/2014, 18:20

... codonoptimised E5 mutants M1 B), M2 C), and M3 D) and analysed after 24 h by confocal laser scanning microscopy using a monoclonal anti-AU1 and polyclonal anticalnexin antibodies Page 11 of 15 (page number ... high-grade cervical lesions and cervical cancer: a meta- analysis Br J Cancer 2003, 89:101-105 Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV: The causal relation between human papillomavirus and ... expression was analysed in each stable polyclone by immunoprecipitation and -blot using mouse monoclonal anti-HA Ab and 500 µg RIPA cell lysate C) FACS analysis of CEM-NKR and CEM-C7 cells transfected...

Ngày tải lên: 18/06/2014, 18:20

... Inc.) Analysis of caspase-8 and caspase-9 using a colorimetric method The activation of caspase-8 and caspase-9 was analyzed using a colorimetric assay kit (KeyGEN) Briefly, mock-infected and HHV- 6A- infected ... was associated with activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP), which is known to be an important substrate for activated caspase-3 Caspase-8 and -9 were also ... IκBα, c-IAP1, c-IAP2 and XIAP β-actin was used as a loading control NF-κB plays a crucial role not only in immunity, inflammation and cell migration but also in cell survival and apoptosis Many studies...

Ngày tải lên: 18/06/2014, 18:20

... particular isolate Data have shown that subtypes A and C are prevalent in Europe, the U.S .A. , and northern Asia Subtypes B and A5 predominate in Africa and the D variant is found in the Pacific ... Maddalena D, Jaffe HW, Weiss RA, et al.: Kaposi's sarcoma-associated herpesvirus and Kaposi's sarcoma in Africa Uganda Kaposi's Sarcoma Study Group Arch Intern Med 1996, 156(2):202-204 128 Mayama ... its primate host and can immortalize infected T-cells [4] Rhadinaviruses can infect ungulates, mice, and rabbits and all share a particular genomic organization characterized by large flanking,...

Ngày tải lên: 19/06/2014, 08:20

... AGG NNNNCNNGNANATNANCACACAACGGTTTGTT GTATTGCTGTTCNAATGTTGTTCCATACAAACT ATAACAATAATGTCTATACTCACTAATTTTAGA ATAAAACTTTAAACATTTATCACATACAGCATA TGGATTCCCATCTCTATATACTATGCATAAATC CCGAAAAGCAAAGTCATATACCTCACGTCGCA ... ACAGTTACTGCGACGTGAGGTATATGACTTTGC TTTTCGGGATTTATGCATAGTATATAGAGATGG GAATCCATATGCTGTATGTGATAAATGTTTAAA GTTTTATTCTAAAATTAGTGAGTATAGACATTAT TGTTATAGTTTGTATGGAACAACATTAGAACAG CAATACAACAAACCGTTGTGTGATTTGTTAATT ... CTATTAGCGGAGGCTCCAACGAGGGGTCCACGGACACCACCTCC ACTCACACAACCAACACTCAGAACAATGACTGGTTTTCAAAGCT GACCAGTTCCGCTTTTAGCGGTCTTTTCGGCGCTCTTCTTGCTGAC AAGAAAACCGAGGAGACCACTCTTCTCGAGGACCGCATCCTCAC TACCCGCAACGGACACACGACCTCGACAACCCAGTCGAGCGTTG...

Ngày tải lên: 19/06/2014, 09:09

... parameter adaptation Data management and communications are related capabilities with traditional and autonomous functionality intended to support autonomy and system integration Data acquisition and ... development in Japan, USA, Canada, China, and the EU Robots, as “environmental” guards, have some advantages and disadvantages From one side, having limited “intellectual” abilities, a robot cannot find ... device-level data and control signals, An Approach to Autonomous Control for Space Nuclear Power Systems 107 system-level information and commands, and spacecraft-level status and demands The effective...

Ngày tải lên: 19/06/2014, 11:20