a systematic review and meta analysis
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... read and approved the final manuscript Material and Methods Virus culture and virus detection by real-time PCR Camelpox strain CP-19, CMV strain AD169, HHV-6 strain U1102, SARS coronavirus strain ... Acknowledgements We gratefully acknowledge the excellent technical assistance of Delia Barz and Jung-Won Sim-Bandenburg The authors are grateful to Andreas Kurth for critical reading of the manuscript References ... asthmatic airways is variable and not suitable for normalising mRNA levels Thorax 2002, 57:765-770 Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, Griffiths LR: Beta-actin an unsuitable...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... read and approved the final manuscript Material and Methods Virus culture and virus detection by real-time PCR Camelpox strain CP-19, CMV strain AD169, HHV-6 strain U1102, SARS coronavirus strain ... Acknowledgements We gratefully acknowledge the excellent technical assistance of Delia Barz and Jung-Won Sim-Bandenburg The authors are grateful to Andreas Kurth for critical reading of the manuscript References ... asthmatic airways is variable and not suitable for normalising mRNA levels Thorax 2002, 57:765-770 Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, Griffiths LR: Beta-actin an unsuitable...
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Báo cáo y học: " Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells" docx
... Pharmacol 2000, 58:145-151 Shankavaram UT, Lai WC, Netzel-Arnett S, Mangan PR, Ardans JA, Caterina N, et al.: Monocyte membrane type 1-matrix metalloproteinase Prostaglandin-dependent regulation ... following activation with 1.5 mM p-aminophenyl mercuric acetate (APMA) Statistical analysis Data were expressed as mean ± SEM or 95% confidence interval (95% CI) and analysed using GraphPad Prism ... stimulation with calcium and calmodulin [34] and it is now recognized that the cAMP-protein kinase A pathway regulates the expression [38] and catalytic activity of PDE4 variants [10,13] as well as...
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Báo cáo y học: " Ancient, independent evolution and distinct molecular features of the novel human T-lymphotropic virus type 4" pot
... TGACAGGGACAACGACCCTCTCCCAGGGGCGACAGCAAGCCCCCAAGGACAAAA c-Myb CTAGCAGGGACTAGTCATCAGCCAAAAAGGTCAACTGTCTCACACAAATAAGGAT bZIP910 Distal 21R in PTLV-1/2 CCGAAGGTTCTGACGTCCCAGCCCAGCCTCAAAACCAGGAAATCCATAGAAATGC ... CCGAAGGTTCTGACGTCCCAGCCCAGCCTCAAAACCAGGAAATCCATAGAAATGC Middle 21R ACCTCGCCCTTACCCACTTCCCCTATCATGAAAAACAAAGGCTGTGACGACTACCC Proximal 21R CCTTCCCCAAAAAATTTGCTTAAACCATCAATAAAGACAGCCTAGCCTATATAAGC TATA box poly (A) signal U3 R ATGAGGATGGTTCAGGAGGGGGCTCGCTCTCTTGCCGATCGCCCTGCTCACCTCGA ... the env-tax fragment are 1863EF1 5'CCTGCC AAAACCT GATCACC TATTC3' and 1863TR1 5'CGACAAC TCGTCCATC GATGG3' and 1863EF2 5'CCCTGTATCTCTTCCCACACTGGGTA C3' and 1863TR2 5'GGG GAGCATAATC CACCGGAGATGG3',...
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AC R Sample Task Type 4 Task
... climate Complementary species Start of active period Number of generations per year French 2.5 cm cool Spanish late spring 1 2 Spanish 1.25 cm 9 10 11 South African ... Sample task type 4 Question 9 – 13 Complete the table below. Choose NO MORE THAN THREE WORDS from the passage for each answer. Write your answers in boxes 913 on your answer sheet. ... 2.5 cm cool Spanish late spring 1 2 Spanish 1.25 cm 9 10 11 South African ball roller 12 13 ……… ...
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A comparative study of discourse structures and some major linguistic features of international declarations and international conventions on human rights part 4
... Education shall be free, at least in the elementary and fundamental stages Elementary education shall be compulsory Technical and professional education shall be made generally available and ... financial assistance in case of need; (c) Make higher education accessible to all on the basis of capacity by every appropriate means; (d) Make educational and vocational information and guidance ... from all forms of sexual exploitation and sexual abuse For these purposes, States Parties shall in particular take all appropriate national, bilateral and multilateral measures to prevent: (a) ...
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Tài liệu E-Human Resource Management 4 pptx
... internally and externally Learning both at individual and at organizational levels will also have to be of a high order in order to facilitate the necessary continuous improvement and innovation ... strategic change emphasize the importance of having an understanding of the organization climate and culture, and the current attitude of employees and management in order to assess the best way ... play in the development of organizational learning The moves we have outlined above towards a new balance between innovation, trust, and learning on the one hand and new forms of discipline and...
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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot
... CATAGTGGAAAGAGGGATATAAATTATCGCATAAAACAATAAACAAAAAGAAAAATG AGGGAACAAAAGCTGGAG GGGAGTCAGCTGTTCGGTAACAGCATCTTGCATAGGCACATCTAAATCTGTATCCTGTAGGGCGAATTGGG GCAAAGTAAAACAGCACGAAAAAAGTGATTACAAATTTCAAGGGAGATATGATGAGGGAACAAAGGCTGGAG ... GGCAATTGGAGTGACATAGCAGCTACTACAACTACAAAAGCAAAATCTCCACAAAGTAAT CGGATCCCCGGGTTAATTAA CCAAGTGCTTCAATCCTAGAGAAGAAGAAAGGTAAGAAAAAGAAAGGAAAGCAACTTAAT GAATTCGAGCTCGTTTAAAC pFA 6a- HIS3MX6 pFA 6a- HIS3MX6 pFA 6a- 13Myc-HIS3MX6 ... GTAACTTCAGGTAGTAACTGGGCCTTGTATAGCCTTTCTAAACATTCGTCCAACTGTAGGGCGAATTGGG GAAATACTATTGAAGCTCAAAAACATCCATAATAAAAGGAACAATAACAATGGTAAGGGAACAAAAGCTGGAG GCGCCTGGCATTTCTTTATTGTTTCAAGCCATTCGTCGGGGCCTCTAGACTGTAGGGCGAATTGGG GGCAATTGGAGTGACATAGCAGCTACTACAACTACAAAAGCAAAATCTCCACAAAGTAAT...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... and N130D variants of human arginase I using an antibody raised against E coli agmatinase (AUH) The relative molecular masses of the protein markers (ST), ovoalbumin (42 700) and carboanhydrase ... inhibitions Also as expected for reactions catalyzed by the same molecular entity, the thermal inactivations of arginase and agmatinase activities of the N130D variant were totally coincident (Fig 3A) Any ... The Authors Journal compilation ª 2006 FEBS ´ R Alarcon et al Agmatinase activity of the N130D mutant arginase I Fig Inhibition of wild-type and N130D variants of human arginase I by agmatine (A) ...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... (BX-9000; KEYENCE, Osaka, Japan) DNA helicase and ATPase activities of the DNA helicases A human MCM4/6/7 complex was prepared, and its DNA helicase activity was measured, as reported previously, ... has in vitro helicase activity Mol Cell 31, 287–293 20 Chino M, Nishikawa K, Tsuchida T, Sawa R, Nakamura H, Nakamura KT, Muraoka Y, Ikeda D, Naganawa H, Sawa T et al (1997) Heliquinomycin, a ... layer chromatography The radioactivity at the sites to which Pi and ATP migrated was measured, and the ratio of the released Pi to ATP was calculated The ratio obtained in the case of the reaction...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... Quimby BB, Alano A, Almashanu S, DeSandro AM, Cowan TM & Fridovich-Keil JL (1997) Characterization of two mutations associated with epimerase-deficiency galactosemia, by use of a yeast expression ... have been compared Disease-causing mutations in human GALE Results Expression and purification of human GALE Human GALE was expressed as an N-terminal hexahistidine fusion protein in E coli and ... epimerase-deficiency galactosaemia J Inherit Metab Dis 21, 341–350 Maceratesi P, Daude N, Dallapiccola B, Novelli G, Allen R, Okano Y & Reichardt J (1998) Human UDPgalactose 4¢ epimerase (GALE)...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped to draft ... been estimated by quantifications of metabolically labelled viral and cellular proteins in the starting material and final preparation [20-22] To estimate these factors for the produced and purified ... centrifugation in a table top Eppendorf centrifuge DNA extraction and quantitative real-time TaqMan PCR Extraction of DNA and determination of viral DNA copies for HHV- 6A was performed as previously...
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Báo cáo sinh học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" docx
... U16-17R gac act cca cca tag atc act c cat gat gca cgc tct acg aga c ctg tga gga act act gtc ttc acg cag cac tcg caa cca ccc tat cag cca gcn cac aaa ggn ata gga gg acb acy gcn cct tch cct ttc ccc aat ... cells are liver macrophages and Hepatocytes are liver endothelial cells 2B-cells priate primer set (Table 2) A band of about 400 bp was seen after PCR analysis, indicating HHV- 6A was replicating ... presence of HIV1 and HCV in the same cell may greatly aggravate the malady Our TEM analysis showed HHV- 6A and HCV in the same cells, with extracellular HIV-1 particles budding or adjacent to the...
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Báo cáo sinh học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L" pot
... of C14 6A, C15 7A, C16 1A, C18 5A, C22 5A, C22 9A 5'-AACCTGGCCAGCAGCAACTACTTCCC-3' and 5'-AACTAGAAGGCACAGTCGAGGCTG-3' were used similarly to produce C32 4A, C34 5A, C37 9A The resultant DNA fragments ... C18 5A) , 5'-GTGCCCCTGGACATCGCCACCAGCATC TGCAAG-3' (F for C22 5A) , 5'-CTTGCAGATGCTGGTGGC GATGTCCAGGGGCAC-3' (R for C22 5A) , 5'-ACCAGCATCGCCAAGTACCCCGACTACATC-3' (F for C22 9A) , 5'-ATGTAGTCGGGGTACTTGGCGATGCTGGTGCAGAT ... 5'-CGACAACAGGGAGGCCATCAGCATGGACTACAAG3' (F for C14 6A) , 5'-GTAGTCCATGCTGATGGCCTCCCTGTTGTCCAC-3' (R for C14 6A) , 5'-CAAGCAGACCCAGCTGGCCCTGATCGGCTGCAAG-3' (F for C15 7A) , 5'-CTTGCAGCCGATCAGGGCCAGCTGGGTCTGCTTG-3'...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... codonoptimised E5 mutants M1 B), M2 C), and M3 D) and analysed after 24 h by confocal laser scanning microscopy using a monoclonal anti-AU1 and polyclonal anticalnexin antibodies Page 11 of 15 (page number ... high-grade cervical lesions and cervical cancer: a meta- analysis Br J Cancer 2003, 89:101-105 Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV: The causal relation between human papillomavirus and ... expression was analysed in each stable polyclone by immunoprecipitation and -blot using mouse monoclonal anti-HA Ab and 500 µg RIPA cell lysate C) FACS analysis of CEM-NKR and CEM-C7 cells transfected...
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Báo cáo sinh học: " Human herpesvirus 6A induces apoptosis of primary human fetal astrocytes via both caspase-dependent and -independent pathways" potx
... Inc.) Analysis of caspase-8 and caspase-9 using a colorimetric method The activation of caspase-8 and caspase-9 was analyzed using a colorimetric assay kit (KeyGEN) Briefly, mock-infected and HHV- 6A- infected ... was associated with activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP), which is known to be an important substrate for activated caspase-3 Caspase-8 and -9 were also ... IκBα, c-IAP1, c-IAP2 and XIAP β-actin was used as a loading control NF-κB plays a crucial role not only in immunity, inflammation and cell migration but also in cell survival and apoptosis Many studies...
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Báo cáo sinh học: " Human herpesvirus 8 – A novel human pathogen" potx
... particular isolate Data have shown that subtypes A and C are prevalent in Europe, the U.S .A. , and northern Asia Subtypes B and A5 predominate in Africa and the D variant is found in the Pacific ... Maddalena D, Jaffe HW, Weiss RA, et al.: Kaposi's sarcoma-associated herpesvirus and Kaposi's sarcoma in Africa Uganda Kaposi's Sarcoma Study Group Arch Intern Med 1996, 156(2):202-204 128 Mayama ... its primate host and can immortalize infected T-cells [4] Rhadinaviruses can infect ungulates, mice, and rabbits and all share a particular genomic organization characterized by large flanking,...
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nghiên cứu phát hiện sự gắn chèn gene e6 và e7 của human papilomavirus type 16 vào bộ gene người bằng kỹ thuật multiplex rt-pcr
... AGG NNNNCNNGNANATNANCACACAACGGTTTGTT GTATTGCTGTTCNAATGTTGTTCCATACAAACT ATAACAATAATGTCTATACTCACTAATTTTAGA ATAAAACTTTAAACATTTATCACATACAGCATA TGGATTCCCATCTCTATATACTATGCATAAATC CCGAAAAGCAAAGTCATATACCTCACGTCGCA ... ACAGTTACTGCGACGTGAGGTATATGACTTTGC TTTTCGGGATTTATGCATAGTATATAGAGATGG GAATCCATATGCTGTATGTGATAAATGTTTAAA GTTTTATTCTAAAATTAGTGAGTATAGACATTAT TGTTATAGTTTGTATGGAACAACATTAGAACAG CAATACAACAAACCGTTGTGTGATTTGTTAATT ... CTATTAGCGGAGGCTCCAACGAGGGGTCCACGGACACCACCTCC ACTCACACAACCAACACTCAGAACAATGACTGGTTTTCAAAGCT GACCAGTTCCGCTTTTAGCGGTCTTTTCGGCGCTCTTCTTGCTGAC AAGAAAACCGAGGAGACCACTCTTCTCGAGGACCGCATCCTCAC TACCCGCAACGGACACACGACCTCGACAACCCAGTCGAGCGTTG...
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Nuclear Power Control, Reliability and Human Factors Part 4 docx
... parameter adaptation Data management and communications are related capabilities with traditional and autonomous functionality intended to support autonomy and system integration Data acquisition and ... development in Japan, USA, Canada, China, and the EU Robots, as “environmental” guards, have some advantages and disadvantages From one side, having limited “intellectual” abilities, a robot cannot find ... device-level data and control signals, An Approach to Autonomous Control for Space Nuclear Power Systems 107 system-level information and commands, and spacecraft-level status and demands The effective...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped to draft ... been estimated by quantifications of metabolically labelled viral and cellular proteins in the starting material and final preparation [20-22] To estimate these factors for the produced and purified ... centrifugation in a table top Eppendorf centrifuge DNA extraction and quantitative real-time TaqMan PCR Extraction of DNA and determination of viral DNA copies for HHV- 6A was performed as previously...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose