a sequence of actions vs a sequence of assertions

... English and Vietnamese Thanks to both qualitative and quantiative approaches, the researcher can describe and analyze, then makes comparison and draws conclusions in order to reach the goals already ... subject And a positive declarative generally takes a negative tag-question whereas a negative declarative generally takes a positive tag-question, as follows: [7] I can’t be sure, can I? [8] And ... characteristics of insertion sequence in terms of syntax and pragmatics in English and Vietnamese? What are the differences and similarities of insertion sequence in terms of syntax and pragmatics in...

Ngày tải lên: 26/11/2013, 13:16

... complex interactions of p53 with target DNA: we learn as we go Biochem Cell Biol 81, 141–150 12 Palecek E, Brazda V, Jagelska E, Pecinka P, Karlovska L & Brazdova M (2004) Enhancement of p53 sequencespecific ... Nakagawara A, Sakuma Y, Kimura S, Ikeda T, Satoh M, Takahashi N, Sato N & Mori M (2000) p73: structure and function Pathol Int 50, 589–593 23 Levrero M, De Laurenzi V, Costanzo A, Gong J, Wang ... AC, Frederick CA & Lippard SJ (1995) Crystal structure of double-stranded DNA containing the major adduct of the anticancer drug cisplatin Nature 377, 649–652 29 Zlatanova J, Yaneva J & Leuba...

Ngày tải lên: 19/02/2014, 05:20

... Insalaco, A. , Al-Ghonaium, A. , Soresina, A. R., Loubser, M., Avanzini, M .A. , Marconi, M., Badolato, R., Ugazio, A. G., Levy, Y., Catalan, N., Durandy, A. , Tbakhi, A. , Notarangelo, L.D & Plebani, A ... the final preparation as assessed by E-Toxate assay (Sigma) A PhD-12 phage library was prepared and expanded according to manufacturer’s directions (New England Biolabs) A single well of a six-well ... Morelli, A. E., Larregina, A. T., Ganster, R.W., Zahorchak, A. F., Plowey, J.M., Takayama, T., Logar, A. J., Robbins, P.D., Falo, L.D & Thomson, A. W (2000) Recombinant adenovirus induces maturation of...

Ngày tải lên: 20/02/2014, 11:20

... mutant E1 In all assays, the E1aS283C and E1aF26 6A mutants behaved essentially the same as wild-type E1 In the DCPIP assay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about ... Kinetic parameters of wild-type and mutant E1s determined by the DCPIP assay Pyruvate ThDP kcat (s)1) Wild-type E1aF26 6A E1aR26 7A E1aD27 6A E1aY28 1A E1aR28 2A E1aS283C E1aY28 1A/ R28 2A/ S28 3A E1aY28 1A/ R28 2A ... that of wild-type E1; mutants with a replacement of E1aY281 tended to lose catalytic activity at a slightly lower temperature than mutants retaining that residue The E1aR26 7A mutant displayed a...

Ngày tải lên: 20/02/2014, 23:20

... SUPPORT2 dataset and RHC dataset (we omit classification accuracy due to lack of space) The thresholds are again chosen at 25% lower quantile, median, and 75% upper quantile of the population survival ... model and the Aalen model have classification accuracies and MSE that are similar to one another on this dataset All regression models (MTLR, Cox, Aalen) beat the baseline prediction using median ... survival time can be appropriate In this case we can measure the loss Table 2: Classification accuracy and MSE of survival probability predictions on cancer registry dataset (standard error of 5CV shown...

Ngày tải lên: 06/03/2014, 02:21

... CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing EMBO J 24, 1988–1998 41 Waldman AS, Tran H, Goldsmith EC & Resnick MA (1999) Long inverted repeats are an ... implicated as the mediator of constitutional t(11;22) translocation in humans [38] Although there are also a large number of (CA)n and (GA)n repeats in the human genome, the frequency of their ... procollagen exon is regulated by the combination of a weak 5¢ splice site and an adjacent intronic stem-loop cis element J Biol Chem 280, 32700–32711 Baraniak AP, Lasda EL, Wagner EJ & Garcia-Blanco...

Ngày tải lên: 07/03/2014, 00:20

... Allowing two substitutions Allowing two substitutions Allowing one substitution AAAGACATG AAAGACAGG AAAGAGATT AAAGAGATG AAAGACATA AAAGACATA AAAGAGAAC AAAGACATA AAAGAAAAG AAACAGATG AAAGAAAAG AAAGAAAAG ... AAAGACATG AGAGACATG AAGGACATG AAATACATG AAAGCCATG AAAGAGATG AAAGACCTG AAAGACACG AAAGACATA + ND ND – – + ND ND ND + – – – – + – + – 0.25 1.5 p=0.01 60_HI 60M_HI Fig The caspase-1 gene upstream ... USA) In addition, oligonucleotides, namely AGAGACATG (P2), AAGGACATG (P3), AAATACATG (P4), AAAGCCATG (P5), AA AGAGATG (P6), AAAGACCTG (P7), AAAGACACG 3896 Cloning of the Hippi pDED domain and N-terminal...

Ngày tải lên: 07/03/2014, 10:20

... shown Black geometrical shapes are additional domains, as indicated ANOGA, Anopheles gambiae; ASHGOS, Ashbya gossypii; BRARE, Brachydanio rerio; CANDGLA, Candida glabrata; CIONA, Ciona intestinalis; ... DIDO-related apoptosis occurs as a result of alterations in DNA regulation caused by chromatin instability Computational analyses Although all splice variants share common domains, long regions of ... DL, Jacobsen BM, Flodin A & Skalnik DG (2000) Cloning of a mammalian transcriptional activator that binds unmethylated CpG motifs and shares a CXXC domain with DNA methyltransferase, human trithorax,...

Ngày tải lên: 07/03/2014, 21:20

... hydrophobic core of the signal sequence Tetracycline-resistant prlA+ and prlA4 Table Bacterial strains and plasmids used in this study Camr and Ampr indicate resistance to chloramphenicol and ampicillin, ... Dynamics) after scanning of the autoradiogram In vitro transcription, translation, targeting and cross-linking analysis To generate truncated mRNA, plasmids encoding truncated nascent chains of FtsQ ... trichloroacetic acid and analyzed by SDS/PAGE and autoradiography The precursor and mature forms of PhoE are indicated by p and m, respectively is inefficiently translocated across the inner membrane...

Ngày tải lên: 08/03/2014, 09:20

... www.animationmeat.com Preparing for Animation: The Art of Thumbnailing The Art of Thumbnailing PDF produced by www.animationmeat.com Preparing for Animation: The Art of Thumbnailing The Art of ... www.animationmeat.com Preparing for Animation: The Art of Thumbnailing The Art of Thumbnailing PDF produced by www.animationmeat.com Preparing for Animation: The Art of Thumbnailing The Art of Thumbnailing ... www.animationmeat.com Preparing for Animation: The Art of Thumbnailing 11 The Art of Thumbnailing PDF produced by www.animationmeat.com Preparing for Animation: The Art of Thumbnailing 12 The Art...

Ngày tải lên: 08/03/2014, 11:20

... Arrowheads and arrows mark the positions of the lac operators The names of the plasmid DNAs are given for each panel (A) IEL analysis of the chromatin assembled over plasmids d10 (lanes 1–7) and ... 14530–14535 Shivaswamy S, Kassavetis GA & Bhargava P (2004) High-level activation of transcription of the yeast U6 snRNA gene in chromatin by the basal RNA polymerase III transcription factor TFIIIC ... binding of proteins at singular sites in an array of densely packed and equally spaced nucleosomes can cause substantial decondensation and rearrangements in condensed chromatin regions, working as...

Ngày tải lên: 16/03/2014, 10:20

... in Table As we were unable to detect animals with phenotype a2 in all tested farm populations, we could only perform three available types of crosses: a1 · a1 , a1 a2 · a1 a2, and a1 · a1 a2 As expected, ... phenotype a2 was revealed after mating the a1 a2 individuals In the offspring of such parents, we found three categories of phenotypes: a1 , a1 a2 and a2 in a ratio : : The data obtained from all crosses ... histone H1 .a As in the case of the nonallelic variants [20], it seems that the alterations in molecular structure of allelic H1 isoforms may affect their affinity to DNA and chromatin, and their ability...

Ngày tải lên: 16/03/2014, 13:20

... supports relational data as well as sequencedata, using a novel design paradigm of enhancedabstractdata types (BADTs ) The systemimplementation basedon this paradigmallows sequence relational queriesto ... on Management of Data, May 1994 [CS92] RakeshChandmand Arie Segev.ManagingTemporalFinancial Data in anExtensible Database.In Proceedings of the International Conference on Very Large Databases(VWB), ... optimization abilities that will translatea languageexpressioninto a query evaluationplan in someevaluation algebra Query Evaluation: If a query language is specified, the EADT must provide the ability...

Ngày tải lên: 16/03/2014, 16:20

... GCCGGGATCCATGGGCAAGCAGAATAGCGCACTGCGGCCAGACAAG GCCGGGATCCATGGGCAAGGCAGCATCTGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCAAGGCAGCATCTAAGGCAGCAGCAGACAAGCCTGTAGCC AATTAACCCTCACTAAAGGG GCCGGGATCCTAGGGCGAATTGGGTACC Ó FEBS 2004 Analysis ... ATATGGATCCATGGGCGCGGCAGCAGCGGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCGCAGCAGCATCTGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCGCAGCAGCANNKGCAGCAGCAGCAGAC ATATGGATCCATGGGCNNKGCAGCAGCGGCAGCAGCAGCAGAC GCCGCTCGAGCCTGTAGCCCATGTT ... GCCGCTCGAGCCTGTAGCCCATGTT AATTCTCGAGTGCTGCTGCTGCCGATGCTGC AATTCTCGAGTGCTGCTGCTGCCGTTGCTGC AATTCTCGAGTGCTGCTGCTGCGAATGCTGC GCCGGGATCCATGGGCAAAACGCTGAGCAAAGAGGACAAGCTCGAG GCCGGGATCCATGGGCAAGCAGAATAGCGCACTGCGGCCAGACAAG...

Ngày tải lên: 16/03/2014, 16:20

... Clostridium acetobutylicum ATCC 824: AAK81366, ORF CAC3436; Clostridium stercorarium: AAC28125, arabinofuranosidase; Cytophaga xylanolytica: AAC38456, arabinofuranosidase I; AAC38457, arabinofuranosidase ... (BH1874); Bacillus subtilis ssp subtilis str 168: CAA61937, arabinofuranosidase 1; CAA99576, arabinofuranosidase 2; Bacteroides ovatus: AAA50391, arabinosidase 1; AAA50393, arabinosidase 2; Bifidobacterium ... NCC2705: AAN24035, BL0181; AAN24368, AbfA1; AAN24945, BL1138; AAN24971, AbfA2; AAN25400, AbfA3; Caulobacter crescentus CB15: AAK23403, ORF CC1422; Cellvibrio japonicus: AAK84947, arabinofuranosidase;...

Ngày tải lên: 17/03/2014, 10:20

... used as a template with the following primers : R450AUp (5¢-GACGAGTACACGGT GGCCGGATACAACCTCAGGA-3¢) and R450ALo (5¢-TC CTGAGGTTGTATCCGGCCACCGTGTACTCGTC-3¢); Y452AUp (5¢-ACACGGTGCGCGGAGCCAACCTCAAG ... (5¢-ACACGGTGCGCGGAGCCAACCTCAAG ACGTC-3¢) and Y452ALo (5¢-GACGTCCTGAGGTTGG CTCCGGCCACCGTGT-3¢); RA+YAUp (5¢-ACACGGT GGCCGGAGCCAACCTCAGGACGTC-3¢) and RA+YA Lo (5¢-GACGTCCTGAGGTTGGCTCCGGCCACCGTG T-3¢) After mutagenesis and sequence ... Data Bank database under the accession number 3NJT Overlay assay Channel analysis Fha30 is a 30-kDa, secreted FHA truncate encompassing the TPS domain and first three repeats It was used as bait...

Ngày tải lên: 23/03/2014, 03:20

... A A A A A AT T TA A G AT C T Mut A AT T T G A A C T G T G A A G AT G C T G G G A G A A A A A AT T TA A G AT C T Mut B AT T T G A A C T G T G C C A GGAC T G G G A G A A A A A AT T TA A G AT C T ... T Mut C AT T T G A A C T G T G C C A AT G A G A G G A G A A A A A AT T TA A G AT C T Mut D AT T T G A A C T G T G C C A AT G C T G A A G G A A A A A AT T TA A G AT C T Mut E AT T T G A A C T G ... 5¢-GGCGGATCCAAGCCAGTGGTTGTTAAT AC-3¢ and 5¢-GCCTCGAGAATCAAGTGTCCCTGCACC T-3¢ (LIN-54-DN); 5¢-GCGGATCCGAGGTGGTGCCAG CTGAG-3¢, 5¢-GCTCTAGAGAATGGAAGCCGTGCCT G-3¢, 5¢-GCTCTAGATTGGCAGATGCAGCTGAAGTA3¢ and 5¢-GCCTCGAGAATCAAGTGTCCCTGCACCT-3¢...

Ngày tải lên: 23/03/2014, 04:20

... receptor site of Na+ channels A total of 50 nonredundant sequences of Na+ channels available in databases were aligned with CLUSTAL X [45] The segments S5–S6 of domain I and S3–S4 of domain IV from ... binding because of the absence of primary electrostatic attraction [70] Cn12 has a slightly negative charge at physiological pH, and the neuroblastoma cells Fig Multiple alignment of amino-acid sequences ... the same conditions as reported [34] Determination of amino-acid sequence and MS analysis The full amino-acid sequence of the toxin was obtained by direct Edman degradation, using an automatic...

Ngày tải lên: 23/03/2014, 12:20

... apparatus using a PrePak Cartridge 25 · 100 mm (Agilent) casted on a PrepLC Universal Base apparatus (Waters) and a Zorbax 300SB-C18 9.4 · 250 mm (Agilent) Samples were eluted using a linear gradient ... A fit of experimental data with a three-parameter negative exponential curve (R > 0.99, data not shown) gave an apparent rate constant value of 0.54 min)1 for EGF-14, and values in the range 0.22–0.26 ... Derivatization of the amino acid mixture with phenylisothiocyanate was achieved according to the standard protocol of PicoTag system (Waters) Analysis of free amino acids was performed by RP-HPLC on a PicoTag...

Ngày tải lên: 23/03/2014, 13:20

... AGU and DiGIR2DP9.2 AGU band Additional bands result from degradation of RNA during incubation at trans-splicing and RPA hybridization conditions Below; quantitation of RPA of trans-spliced GA ... reactions was approximately identical, the addition of the ribozyme band (RNA 4) in the fractions denominator gave approximately identical comparative results (data not shown) percentage) of ... reprogrammed product (RNA 2) + target GA RNA (RNA 3) The amount of trans-splicing ribozymes that had undergone the reaction [calculated as the amount of reprogrammed product (RNA 2) as a fraction...

Ngày tải lên: 23/03/2014, 13:20