a novel encounter with big data

... the National Health Laboratory Service (authors’ data) All costs are shown in US dollars after applying the 2008 average exchange rate of 8.28 South African rands to one US dollar [5] An annual ... Discount rate applied 3.0% to future costs (annual) Exchange rate (Rand/$) 8.28‡ *Long et al, 2010 [5] † Authors’ data from National Health Laboratory Service, South Africa ‡ Exchange rate taken from ... second-line antiretroviral therapy for HIV/AIDS in South Africa AIDS 2010, 24:915-919 Fox MP, Brennan A, Maskew M, MacPhail P, Sanne I: Using vital registration data to update mortality among patients...

Ngày tải lên: 20/06/2014, 08:20

... of KB validates ϕ If KB |= C (a) then we say that a is an instance of C w.r.t KB 2.2 Automaton-Modal Operators Given an interpretation I and a finite automaton A over alphabet R, define AI = { x, ... following: A = R, QA , qA , A , FA If q is a state of a finite automaton A then by Aq we denote the finite automaton obtained from A by replacing the initial state by q Lemma Let I be a model of a regular ... R is a regular RBox with the following RIA-automatonspecification: Note that Ar = (Ar )0 and Ar = (Ar )0 Consider the Horn-RegI knowledge base KB = R, T , A with A = {A( a), B (a) , A( b), r (a, b)}...

Ngày tải lên: 13/08/2015, 10:00

... 1145-9 Tamura N, Ogawa Y, Chusho H, et al Cardiac fibrosis in mice lacking brain natriuretic peptide Proc Natl Acad Sci USA 2000; 97: 4239-44 Mukoyama M, Nakao K, Saito Y, et al Human brain natriuretic ... like to thank Dr Y Watanabe and Dr Y Izumi for collecting the samples, and Ms H Tobe, M Nakamura, and K Sugama for their technical assistance This work was supported financially by a grant from ... Japanese Circ Res 2000; 86: 841-5 13 Nakayama T, Soma M, Rahmutula D, Ozawa Y, Kanmatsuse K Isolation of the 5'-flanking region of genes by thermal asymmetric interlaced polymerase chain reaction...

Ngày tải lên: 26/10/2012, 10:04

... Yamaguchi H, Sasaki K, Satomi Y, Shimbara T, Kageyama H, Mondal MS, Toshinai K, Date Y, Gonzalez LJ, Shioda S, Takao T, Nakazato M, Minamino N Peptidomic identification and biological validation of ... Lange DJ, Voustianiouk A, Macgrogan D, Ho L, Suh J, Humala N, Thiyagarajan M, Wang J, Pasinetti GM A ketogenic diet as a potential novel therapeutic intervention in amyotrophic lateral sclerosis ... Cruz Biotech, CA) The assay was developed using a stabilized HRP substrate All samples were analyzed in the linear range of the ELISA using over-expressed human Vgf as a standard Assessment of...

Ngày tải lên: 03/11/2012, 10:52

... plants Microalgae cultivation and reclamation Wastewater Artificial wetland Disinfection Primary settled sludge Microalgal cells Organics in high concentration Oil extraction Environmental and ... centrifugation, and filtration, could be applied By separating microalgal cells from the wastewater, clean water with low content of organics and inorganic nutrients could be obtained, and also the ... very important in the microalgal cultivation unit of the novel process Harvest of microalgal biomass The microalgal biomass needs to be separated and harvested from the microalgal cultivation unit...

Ngày tải lên: 05/09/2013, 10:17

... build the data adapter ' and fill the data table Dim odaResults As _ New OleDb.OleDbDataAdapter("Select * From MyProdAndCat", BuildCnnStr("(local)", "Northwind")) odaResults.Fill(dtResults) Catch ... odaResults.Fill(dtResults) Catch excp As Exception MessageBox.Show(excp.Message) Exit Sub End Try ' Assign the data table to the data grid's DataSource property Me.dgResults.DataSource = dtResults End Sub ... DataSource property is set to the data table that was filled Listing 6.17 frmHowTo6_7.vb: Loading the Form Private Sub btnExecute_Click(ByVal sender As System.Object, ByVal e As System.EventArgs)...

Ngày tải lên: 21/01/2014, 12:20

... analysis, data architecture, data modeling, data migration and integration, ETL, data quality, data cleansing, business intelligence, database administration, data mining, and enterprise data warehousing ... 10 years He began his career as a Database Application Programmer and quickly developed a passion for the SQL language, data processing, and analysis He entered the realm of BI and data warehousing ... data Job roles that are a likely match include ETL developers, Data Warehouse Specialists, Business Intelligence Analysts, Database Administrators, Database Programmers, Enterprise, or Data Architect,...

Ngày tải lên: 12/02/2014, 12:20

... TCATCAT-3¢; rOMM-64-IV, 5¢-CGCGGATCCGCCCCT GTTAATGATGGAACC-3¢ and 5¢-CGCCTCGAGCTAA GAAGACTGGGCTGCCAG-3¢; rOMM-64-V, 5¢-CGCGG ATCCAGGCAAGATTTTAAGCATCCA-3¢ and 5¢-CGCC TCCACCTAAGAGGCATCCTTGTCCAC-3¢; ... 5¢-CGCCTCCA CCTAAGAGGCATCCTTGTCCAC-3¢; rOMM-64-II, 5¢CGCGGATCCACCGTAGACACTTATGATATA-3¢ and 5¢-CGCCTCGAGCTAAGAGTCAGCTTGCACGTC-3¢; rOMM-64-III, 5¢-CGCGGATCCGCTGATGTGACCAGT GATGAC-3¢ and 5¢-CGCCTCGAGCTATTTGGGCTCTT ... DNA contamination in the total RNA was confirmed by lack of amplification of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCATCGGCAACGAGAG-3¢ and 5¢-TGGAGTTGTAGGTGGTCTCGTG-3¢) without...

Ngày tải lên: 18/02/2014, 17:20

... mitochondria was analyzed by thiobarbituric acid assay [34] In this assay, thiobarbituric acid reacts with malonaldehyde and or other carbonyl by-products of free-radicalmediated lipid peroxidation ... thiobarbituric acid reactive substances (TBARS) as a marker for lipid peroxidation, and 6-CySeCD, 6-SeCD and Ebselen as antioxidants in ferrous sulfate ascorbate-induced mitochondrial damage ... EDTA, 0.25 mm NAPDH, unit GSH reductase and lm 6-CySeCD Background absorption of the noncatalytic reaction was run without mimic and was subtracted Kinetic data were analyzed by double-reciprocal...

Ngày tải lên: 19/02/2014, 02:20

... for caspases and 7; Ac-YVAD-AMC is a substrate for caspase 1; Ac-IETD-AMC is a substrate for caspase and 10; Ac-LEHD-AMC is a substrate for caspases 2, 4, and Ac-DVPD-AMC, Ac-DPSD-AMC and Ac-ESQD-AMC ... caspase 1, WEHD-AMC; caspase 2, VDVADAMC; caspase 3, DEVD-AMC; caspase 4, WEHD-AMC; caspase 5, WEHD-AMC, caspase 6, VEID-AMC; caspase 7, DEVD-AMC; caspase 8, IETD-AMC; caspase 9, LEHD-AMC; caspase ... DVPD-AMC are increased when apoptosis is induced In contrast, cleavage of the p27KIP1 DPSD-AMC substrate remained constant under these assay conditions Thus it appears that KIPase maintains a basal...

Ngày tải lên: 19/02/2014, 13:20

... cross-react with antibodies against plant helicases including PDH45 and PDH65 and also against human DNA helicases I, II, III and IV (data not shown) ssDNA-dependent ATPase activity was present at a ... 2A, lane 4) and ssDNA-dependent ATPase activity (data not shown) sedimented together between alcohol dehydrogenase and BSA (fraction 11) and gave a molecular mass of 120 kDa with a sedimentation ... substrates (Fig 5G and H) were prepared as described previously [11,15] 32 ATP-dependent DNA helicase and DNA-dependent ATPase assays The standard DNA helicase reaction was performed in a 10-lL reaction...

Ngày tải lên: 20/02/2014, 11:20

... CGCTGGTTCTTGCCAGTCTCAGTGCCGTGGTTGCTAGGGAT TTTAGCACCACGAGCCTGGTCACCGCAACGCTGAGC CGCAGAAGCCGTACTTACCACAGCACAGGCAGTTCG GACTGGCAAGAACCAGCGCCACAGTAAGCGTCACCA GCTAGGATCCCTAGCAACCACGGCAC Table Antifungal activity ... Escherichia coli and assays of recombinant WAMP 1a activity against diverse plant pathogens, such as chitin-containing and chitin-free fungi, and Grampositive and Gram-negative bacteria The amino acid ... activity of peptides was assayed against several Gram-positive and Gram-negative bacteria using radial diffusion assay Petri dishes with Luria–Bertani agar were seeded with test bacteria The peptide...

Ngày tải lên: 07/03/2014, 02:20

... GACCTCATGACGGCGGACGTGGACCG Down: CGGTCCACGTCCGCCGTCATGAGGTC Ser64 fi Ala Glu168 fi Ala Val169 fi Ala His170 fi Ala Asn189 fi Ala Arg191 fi Ala Asp192 fi Ala Leu193 fi Ala Leu196 fi Ala The concentrations ... fi Ala Up: CAAGCGCGCTAGTGCTTCGGCAGGCG Down: CGCCTGCCGAAGCACTAGCGCGCTTG Up: CGCTAGTCCTGCGGCAGGCGCATTGG Down: CCAATGCGCCTGCCGCAGGACTAGCG Up: CAGCACTCGTCGCGGTCCATACCGAG Down: CTCGGTATGGACCGCGACGAGTGCTG ... Up: CACTCGTCGAGGCCCATACCGAGCAG Down: CTGCTCGGTATGGGCCTCGACGAGTG Up: CGTCGAGGTCGCTACCGAGCAGGAAG Down: CTTCCTGCTCGGTAGCGACCTCGACG Up: GGTGATTGGCGTTGCCGCCCGCGACC Down: GGTCGCGGGCGGCAACGCCAATCACC...

Ngày tải lên: 07/03/2014, 03:20

... CGA ATT CCT TTC TTT CTT TCT TTC TTT CTT TCT TTC TTT CTT TCT TTC TTT TTT TTT TTC TCG AGC CCC-3¢; antisense: 5¢-GGG GCT CGA GAA AAA AAA AAA GAA AGA AAG AAA GAA AGA AAG AAA GAA AGA AAG AAA GAA AGG ... stabilize ARE-containing mRNAs [68] and has been associated with the activation of c-Jun N-terminal kinase (JNK) [69] Similarly, activation of MAP kinase-activated protein kinase has been associated ... formaldehyde gel After transfer on a nylon membrane by capillary blot the RNA was hybridized with a 32P-radiolabelled MARCKS cDNA probe (p809.1) [22], washed and exposed to X-ray film (Kodak AR) with...

Ngày tải lên: 08/03/2014, 08:20

... analysis, data architecture, data modeling, data migration and integration, ETL, data quality, data cleansing, business intelligence, database administration, data mining, and enterprise data warehousing ... 10 years He began his career as a Database Application Programmer and quickly developed a passion for the SQL language, data processing, and analysis He entered the realm of BI and data warehousing ... data Job roles that are a likely match include ETL developers, Data Warehouse Specialists, Business Intelligence Analysts, Database Administrators, Database Programmers, Enterprise, or Data Architect,...

Ngày tải lên: 14/03/2014, 18:20

... has a point mutation at amino acid 408, was created by PCR-based site-directed mutagenesis using pmGFP–Brox as a template and complementary primers (5¢-CAA AAG GAC ACT GGG TCC TAC ATC TCC TAA ... AAT TCA TGA CCC ATT GGT TTC ATA GGA ACC-3¢ and 5¢-GGG AAT TCT TAG GAG ATG TAG CAC CCA GTG TC-3¢ (nucleotides corresponding to a cDNA of the hypothetical protein C1orf58 are underlined), and an ... Peroxidase-conjugated goat anti-rabbit IgG and goat anti-mouse IgG were obtained from Wako (Osaka, Japan) Preparation of rabbit pAbs against Alix has been described previously [40] Cy3-labeled anti-mouse...

Ngày tải lên: 16/03/2014, 06:20

... data Since the test data tend to have a similar error rate to the narrow standard deviation, we computed the overall performance over the average word spacing error rate, which is 9.1% The baseline ... test data and randomly insert spacing errors from 0% to 20% in the same way in which we made the development data We feel that this strategy can model both the intentional and un-intentional human ... using the relative frequency information of the training data, and a smoothing technique is applied to relieve the datasparseness problem which is the linear interpolation of n-grams that are used...

Ngày tải lên: 17/03/2014, 02:20

... relational database, so that the geospatial coordinate data of the GIS data layers are stored in the relational data tables Since the relational database supports relationships between its tables, ... spatial and temporal data, complex analyses, and visualization, (Matejicek, 2002) Due to the ability to manage a number of spatial and temporal data formats, data structures created in the framework ... values of variables are transferred and incorporated into the GIS database, which can be useful in managing data time series To accommodate large data sets and many variables such as air quality...

Ngày tải lên: 23/03/2014, 02:20

... Na+ channels available in databases were aligned with CLUSTAL X [45] The segments S5–S6 of domain I and S3–S4 of domain IV from selected sequences are shown (NaV1.1, 1.4 and 1.5 from rat; NaV1.7 ... Fig Final purification and amino-acid sequence determination of Cn12 (A) A sample of fraction II-4 from a CM-cellulose ionexchange column [33] containing mg protein was applied to an analytical C18 ... interferes with cellular communication at the level of the Na+ channels diagram of sequential and medium range data, the chemical shift index, and coupling constants used for the structure calculation...

Ngày tải lên: 23/03/2014, 12:20

... cata ag ATGTCC AAGGAC gt aagt … ttaa ag GATTGC AGAAGA gt aagt … ttgc ag CAATTT ATGTTG gt aagt … tttt ag GGCATA ACTCAA gt aaga … tatc ag GATTTC AAGTAG gt aagt … attt ag CTTGCA CAAAAG gt aaga … ... ag ATGCCC AAGGAC gt aagt … ttca ag GATTGC AGAAGA gt aagt … ttgc ag CAATTT ATGTTG gt gagt … tttt ag GGCATA ACTCAA gt aagg … taat ag GATTTC ATGTAG gt aagt … atgc ag CTTGCA GCAAAG gt aaga … tcta ag ... ag ACGTAT TACTTG gt atgt … aatc ag GATATG ACAGAG gt aaaa … tctc ag AAAATT GCATTG gt aagg … attt ag GGCAGT CATTAT gt aagt … tttc ag GATATT TTGCAG gt ttgt … ttta ag GTTCAA ATGGAC gt atgt … cata...

Ngày tải lên: 23/03/2014, 13:20