a novel concept in innate responses to viral infection
Roles of siRNAs and miRNAs in host responses to virus infection identification and characterization of a novel viral suppressor of RNA silencing
... NS1, are distinct dsRNA-binding proteins and essential for pathogenesis by inhibiting the mammalian interferon-regulated 23 innate antiviral response It was also demonstrated that the dsRNA-binding ... shows that PTGS in plants and RNAi in animals and quelling in Neurospora crassa represent a highly conserved mechanism, indicating an ancient origin (Vance and Vaucheret, 2001; Cogoni and Macino, ... broader group of posttranscriptional gene silencing phenomena common to virtually all eukaryotes PTGS has been demonstrated as a natural antiviral defense in plants Is RNAi also antiviral in animals?...
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Báo cáo y học: "A novel combination of Chinese medicines to treat advanced cancers and lymphomas in rats" ppt
... was measured according to the method by Geng et al [29] Statistical analysis Quantitative data were expressed as mean ± SD (standard deviation) and analyzed by one way analysis of variances (ANOVA) ... Containing unpaired electron, free radicals are highly reactive and cause damage to part of cells by inducing DNA strand breaks, purine oxidation and protein DNA cross linking and cell membrane damage ... glycosuria and alterations in plasma creatine, urine nitrogen, uric acid and creatine clearance, concentration related changes in hematocrit and erythrocytes, as well as reticulocyte and erythroblast...
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Báo cáo khoa học: Gc recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions pot
... extracted, and the target ZI3 1A gene was amplified by PCR with primers 5¢-AAATA TAAAACGCTAGCGTCGACATGGCGC-3¢ and 5¢-AGC GTAAAGGATGGGGAAAG-3¢ The final ratio of target cells was determined by counting ... 875–884 20 Togawa S, Ishii J, Ishikura A, Tanaka T, Ogino C & Kondo A (2010) Importance of asparagine residues at positions 13 and 26 on the amino-terminal domain of human somatostatin receptor subtype-5 ... as various mating responses, including global changes in transcription, a reporter gene assay and mating selection are available (Fig 1A) [12] Unlike stable interactions, however, transient interactions...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... in the Methods section hydrolases (Fig 3) These enzymes share a functional catalytic triad made of a catalytic nucleophile serine, associated to a proton carrier histidine and a charge relaying ... In each case, the kinetics of inactivation of Rv1399c have been monitored by measuring the remaining activity, as a function of time, using tripropionin as a substrate 3958 S Canaan et al (Eur ... substrate recognition, suggesting a preference for a polar noncationic substrate as a natural substrate since Rv1399c does not exhibit any catalytic activity against acetylcholine (Table 2) A structural...
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Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae doc
... LJY430 LJY431 LJY432 MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa Euroscarf [16] ATCC [15] This study ... data indicates that yeast contain a metalloprotease, which could be a novel a- secretase, in addition to the two aspartic proteases, Yps1 and Yps2 None of the yeast metalloproteases contains an ... Biochemicals, Stockton, UK) as substrate, 20 lL yeast extract, various inhibitors and activators in a total volume of 30 lL The mixture was incubated at 30 °C for 2.5 to h and the reaction terminated...
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Báo cáo khoa học: Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress doc
... phosphorylation of a MAPKKK, a MAPKK, and a MAPK [5] The phosphorylation and activation of an MAPK can lead to changes in its subcellular localization and its interaction with transcriptional effectors, ... and negatively mediate pathogen signal transduction [9] In tobacco, salicylic acid (SA)-induced protein kinase and woundinduced protein kinase, two MAPKs in group A, were activated by inoculation ... three interlinked protein kinases: a MAPKKK kinase, a MAPKK, and a MAPK [4] Signals from extracellular stimuli are transmitted into the cell and are sensed by downstream targets via the sequential...
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Strategic Adaptation: Cross-Cultural Differences in Company Responses to an Economic Crisis pot
... adaptation Failure to adapt to a changing environment can lead to fatal consequences This applies not only to the realm of business In a classical analogy, also species in the natural world have ... other hand, the research was mainly based on an empirical study – a quantitative survey among managers in Austria and Slovenia Introduction: Researching strategic adaptation in a cross-cultural context ... A (2004) Cultural variations in strategic issue interpretation: Relating cultural uncertainty avoidance to controllability in discriminating threat and opportunity Strategic Management Journal,...
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Báo cáo khoa học: Light regulation of CaS, a novel phosphoprotein in the thylakoid membrane of Arabidopsis thaliana doc
... for CaS were 5¢-AAATGGCAACGAAGTCTTCAC-3¢ and 5¢-CAGTCGGAGCTAGGAAGGAA-3¢ Isolation of plasma membrane, intact chloroplasts, stroma and thylakoids The plasma membrane fraction of Arabidopsis was ... (AY341888) was amplified by PCR using PfuTurbo CX Hotstart DNA polymerase (Strategene, La Jolla, CA, USA) and the uracil-containing primers nt114 (forward: GGCTTAAUATGGCTATGGCGGAAATGG CAACGA) and ... proteins and FHA domains, according to eukaryotic linear motif prediction at http://www.expasy.org 14-3-3 proteins are known to function as adaptors that mediate protein–protein interactions and to...
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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... decreased innate resistance to HSV-1 infection, depletion of NK cells had no such effect Innate resistance to HSV-1 infection is dependent on Stat Stat is an IFN-activated transcription factor that ... the weakly virulent KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 ... IFN-α/β and IFN-γ are important activators of Stat 1-induced resistance to HSV-1 in vivo However, further studies are necessary to determine if chemokines and pro-inflammatory cytokines are also...
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báo cáo hóa học: " LPS preconditioning redirects TLR signaling following stroke: TRIF-IRF3 plays a seminal role in mediating tolerance to ischemic injury" doc
... detrimental effect of TLR signaling is associated with the pathways that lead to NFB activation and pro-inflammatory responses In contrast, TLR signaling pathways that activate IRFs can induce antiinflammatory ... influencing pro-inflammatory cytokine production [3,31] In particular, administration of the NFB inhibitor Tat-NEMO Binding Domain provided protection against hypoxia-ischemia in neonatal rats ... following injury [4] Stroke also induces an acute inflammatory response in the circulating blood Inflammatory cytokine and chemokine levels, including IL6, IL1b, MCP-1 and TNFa are elevated in the...
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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... decreased innate resistance to HSV-1 infection, depletion of NK cells had no such effect Innate resistance to HSV-1 infection is dependent on Stat Stat is an IFN-activated transcription factor that ... the weakly virulent KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 ... IFN-α/β and IFN-γ are important activators of Stat 1-induced resistance to HSV-1 in vivo However, further studies are necessary to determine if chemokines and pro-inflammatory cytokines are also...
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Desiccant enhanced evaporative air-conditioning (DEVap): evaluation of a new concept in ultra efficient air conditioning docx
... does in building spaces only Today’s A/ C systems: • Maintain a healthy building environment o In commercial and new residential, A/ C provides ventilation air to maintain indoor air quality o A/ C ... remaining refrigerants used today (R41 0A and R-13 4A) are strong contributors to global warming Their global warming potentials are 2000 and 1300, respectively (ASHRAE 2006) Finding data on air ... eliminates desiccant entrainment into the airstream When used to contain water, it eliminates wet surfaces, prevents bacterial growth and mineral buildup, and avoids cooling core degradation DEVap’s...
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Báo cáo y học: "Familial Polycythemia Caused by a Novel Mutation in the Beta Globin Gene: Essential Role of P50 in Evaluation of Familial Polycythemi" potx
... easily available in routine and even reference laboratories Lichtman and colleagues have reported a mathematical formula which can be used to calculate P50 reliably [5] Calculating P50 using this ... these Hb variants are inherited in an autosomal dominant manner High affinity Hb variants release oxygen in the tissue relatively slowly and create relative tissue hypoxia This leads to increased ... polycythemia in her mother, maternal grandmother and her younger sister Physical examination was unremarkable Laboratory parameters were remarkable for elevated hemoglobin (17.2 gm%) and hematocrit...
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báo cáo khoa học: "Bilateral adrenocortical carcinoma in a patient with multiple endocrine neoplasia type 1 (MEN1) and a novel mutation in the MEN1 gene" potx
... adrenal gland Immunohistochemically, the cells were MelanA and synaptophysin immunopositive No immunostaining for cytoceratin, chromogranin, EMA and CEA was observed The remaining non-neoplastic adrenal ... the body and tail of the pancreas and (iv) functioning bilateral adrenal tumors, was established The patient was submitted to an exploratory laparotomy through a bilateral subcostal incision ... histological findings compatible to neuroendocrine tumor, somatostatin receptor imaging with Indium 111 (OctreoScan®) disclosing radionuclide material uptake in the left upper quadrant of the abdominal...
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Báo cáo y học: "Screening of an endothelial cDNA library identifies the C-terminal region of Nedd5 as a novel autoantigen in systemic lupus erythematosus with psychiatric manifestations" ppt
... mechanism by which Nedd5 may be autoantigenic Indeed, apoptosis may play an important role in bypassing tolerance to intracellular autoantigens The specific modification of autoantigens and their ... contribute to an overload of autoantigens in the circulation or in target tissues that could become available to initiate an autoimmune response [29] In susceptible individuals, this can lead to autoantibody-mediated ... G, Alessandri C, Capoano R, Profumo E, Siracusano A, Salvati B, Rigano R, et al.: Screening of a HUAEC cDNA library identifies actin as a candidate autoantigen associated with carotid atherosclerosis...
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Báo cáo y học: "Identification and characterization of the carboxy-terminal region of Sip-1, a novel autoantigen in Behçet''''s disease" docx
... protein Sip1 Sip1 is a nuclear splicing factor containing an arginine/serine-rich domain and a RNAbinding motif that may play a role in linking the processes of transcription and pre-mRNA splicing ... human antibodies (0.1 µg/µl) in PBS containing 1% bovine serum albumin Fluorescein isothiocyanate-conjugated anti-human IgG (Sigma) was added and fluorescence was analysed with an Available online ... Margutti P, Delunardo F, Sorice M, Valesini G, Alessandri C, Capoano R, Profumo E, Siracusano A, Salvati B, Riganò R, Ortona E: Screening of a HUAEC cDNA library identifies actin as a candidate...
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báo cáo khoa học: " A novel mutation in the calcium-sensing receptor gene in an Irish pedigree showing familial hypocalciuric " doc
... normal or elevated PTH levels should have a calcium level determined We also think that DNA sequencing is minimally invasive, is becoming more affordable, can lead to accurate diagnosis, and should ... Postlethwaite W, Sheridan B, Brown RC, Atkinson AB: Familial hypocalciuric hypercalcaemia as a differential diagnosis of hyperparathyroidism: studies in a large kindred and a review of surgical experience ... characterization of a novel mutation in the calcium-sensing receptor gene in familial hypocalciuric hypercalcaemia: modulation of clinical severity by vitamin D status J Clin Endocrinol Metab 2007,...
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báo cáo khoa học: " Roles of arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress" doc
... responses Plant J 2007, 50(2):347-363 Seki M, Ishida J, Narusaka M, Fujita M, Nanjo T, Umezawa T, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Yamaguchi-Shinozaki K, Carninci P, Kawai ... sensitivity to ABA in germination assays Disruption of WRKY18, on the other hand, significantly reduced plant sensitivity to ABA as indicated by an approximate 15% increase in the germination rates ... WRKY40 negatively regulates plant responses to ABA probably by modulating ABA-regulated genes products could act as early transcriptional effectors to regulate expression of additional ABA-induced...
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Báo cáo y học: "A novel mutation in the ADA gene causing severe combined immunodeficiency in an Arab patient: a case report" ppt
... F-50-TGCCTGCTTCCCAGGGTGTCGAAGAGATTT-30 F-50-AGGATCAAAGGCGGGTGAAC-30 R-50-TCCCTCTCTCCAAAGATTCCAG-30 F-50-AGGATCAAAGGCGGGTGAAC-30 R-50-TCCCTCTCTCCAAAGATTCCAG-30 F-50-TCTGAAGCCCAGTCCCAAAG-30 R-50-AAATGTTGCTCAGCCCCAC-30 55 692 55 ... F-50-CCCAAAGCCTCCTCTTCCTCCT-3' F-50-AGGTCTCCAGTTGTTTCATG-30 F-50-TAGGCTGGGAGGTCTCTC-30 R-50-ACCCAACAAAGACACACTC-30 F-50-ATGCTGTTGAAGCAGGCAGCATGACTAGGA-3' F-50-TGCCTGCTTCCCAGGGTGTCGAAGAGATTT-30 F-50-AGGATCAAAGGCGGGTGAAC-30 ... R-50-TGTCCCTGATTAGCCCGCAA-30 F-50-GCAGCCAGCCAGTAAAATG-30 R-50-TGTCCTCACAGTCCCACTTC-30 F-50-GTCCACCACTCACTGTTTTG-30 R-50-AGTCCATCACACCCACATC-3 F-50-TGTTCCCAACCCCTTTCTTCC-30 R-50-AAATGGGCCAGACTCACTTCAG-30...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008