a good model of human disease

... a pattern of progressive adenocarcinoma with similar genetic changes and pathophysiology as seen in human breast cancers associated with BRCA1-mutations [11,12] Additionally, as in human BRCA1-associated ... University of Maryland, Baltimore animal facility and maintained in accordance with institutional guidelines approved by the University of Maryland, Baltimore Animal Care and Use Committee To compare ... enlarged view of a cell indicating the contact surfaces of the source and the drain is shown to the right of the wafer Gate voltage is applied at the back of the wafer Also indicated are the aligning...

Ngày tải lên: 11/08/2014, 00:23

... Pro transmission scanner (Epson, Nagano, Japan) and analyzed with imagemaster 2d platinum software, version 5.0 (GE Healthcare) Spots were detected automatically by the software and manually refined; ... Germany) Capillary voltage was 1.5–2 kV and a dry gas flow rate of 10 LÆmin)1 was used with a temperature of 230 °C The scan range was 300–1800 m ⁄ z The tandem mass spectra were annotated and peak list ... eukaryotic initiation factor 5A (eIF 5A) , parathymosin, L7 ⁄ L12, annexin A2 , annexin A5 , aldolase A, fascin and peroxyredoxin 1] displayed quantitative differences, regardless of whether or not a- synuclein...

Ngày tải lên: 15/02/2014, 01:20

... A total of 30 pairs of a garden-path sentence and its ambiguous, non-garden-path control were tested for a comparison of the probability decrease at the disambiguating region In 80% of the cases, ... increasing ambiguity so that other ambiguity types also can be disambiguated in a similar way via lexical category disambiguation This idea has been explored as one of the lexicalist approaches to ... Cambridge, Massachusetts, 1999 S Narayanan and D Jurafsky A bayesian model predicts human parse preference and reading times in sentence processing Proceedings of Advances in Neural Information Processing...

Ngày tải lên: 20/02/2014, 11:21

... hemiparkinsonian rats Brain Res 2004, 996:237-245 Adams FS, LaRosa FG, Kumar S, Edwards-Prasad J, Kentroti S, Vernadakis A, Freed CR, Prasad KN: Characterization and transplantation of two neuronal cell ... Microbrightfield software and cell density was calculated for striatal and midbrain volumes The striatum was outlined according to anatomical landmarks following the Paxinos atlas[31] To avoid bias in the ... injection was significantly attenuated in the striatum of COXIB treated animals Photomicrographs of activated microglia immunohistochemistry in a representative striatal section of a vehicle (A, B) and...

Ngày tải lên: 19/06/2014, 22:20

... conjugated amyloid β, and fAβ1–42: fibrillar amyloid) Scale bar = 100 microns B Image analysis of thioflavine in hippocampus and cortex of animals immunized at 12–16 months Mean of each animal is ... that reactive microglia are characteristically associated with fibrillar amyloid containing plaques in both human AD and animal models, we assessed the effect of immunization on microglial reactivity ... image analysis, data analysis and presentation, molecular genetic studies and manuscript preparation; RK prepared immunogens and performed immunoassays, S.W performed image analysis, data analysis...

Ngày tải lên: 19/06/2014, 22:20

... neurodegenerative disorders Other authors have documented that pharmacological activation of KATP channels can exert neuroprotective and anti-inflammatory effects on the brain against ischemia, trauma and ... reduction assay was used as an indicator of cell viability MTT (Sigma-Aldrich, St Louis, MO, USA) was added to a well at a final concentration of 0.5 mg/mL After MTT incubation at 37°C, DMSO was added ... EAE animals Diazoxide treatment diminishes the astrocytic reactivity and area of activated microglia/macrophage in the effector phase of EAE To assess the consequences of diazoxide administration...

Ngày tải lên: 19/06/2014, 22:20

... duplicate Statistical analysis Statistical analysis of the data was performed using GraphPad Prism version 5.00 for Windows (GraphPad Software, San Diego California, USA, http://www.graphpad.com) ... apomorphine-induced rotational behaviour was analyzed to assess unilateral degeneration of presynaptic dopaminergic neuron terminals at and 21 days after 6OHDA injection Although rats that had ... Figure BAb exacerbates 6-OHDA-induced behavioural deficits Contralateral rotation measurements following administration of apomorphine in each experimental group are shown in bar graph at days and...

Ngày tải lên: 19/06/2014, 22:20

... 5'-GCCTCCAGCATGAAAGTCTC, 3'-TAAAACAGGGTGTCTGGGGA), IL-1β (5'-TGCACGATGCACCTGTACGA, 3'-AGGCCCAAGGCCACAGGTAT), IL-6 (5'-GTTCCTGCAGAAAAAGGCAAAG, 3'-CTGAGGTGCCCATGCTACATTT), matrix metalloproteinase (MMP)-3 ... (5'-GTCCTCTCCCAAGTCCACACA, 3'-CTGGTCTCAAGTCAGTGTACAGGTAA), CC chemokine ligand (CCL)5 (formerly called RANTES; 5'-CGCTGTCATCCTCATTGCTA, 3'-GCTGTCTCGAACTCCTGACC), CCL2 (formerly called MCP-1; 5'-GCCTCCAGCATGAAAGTCTC, ... A, Ghosh S: Amelioration of acute inflammation by systemic administration of a cell-permeable peptide inhibitor of NF-κB activation Arthritis Rheum 2005, 52:951-958 20 Ariga A, Namekawa J, Matsumoto...

Ngày tải lên: 09/08/2014, 07:20

... The features of Parkinsonian gait are probably manifestations of adaptive mechanisms to avoid unsteadiness and falling during walking In conclusion, the CatWalk-assisted automated gait analysis ... 28:1936-1950 14 Gabriel AF, Marcus MA, Honig WM, Walenkamp GH, Joosten EA: The CatWalk method: a detailed analysis of behavioral changes after acute inflammatory pain in the rat Journal of Neuroscience ... dopaminergic neurons Max area The size of the print area at maximal contact of all four paws during floor contact was smaller in rats weeks after 6-OHDA-lesioning than in sham rats; however, a...

Ngày tải lên: 10/08/2014, 05:21

... that allanxanthone C and macluraxanthone purified from Guttiferaes are capable of in vivo antileukemic effects in a xenograft murine model of human CLL These therapeutic activities of the natural ... treated with daily iv injections (0.2 ml in saline) on days to (arrows) of mg/kg of allanxanthone C (black triangle) or macluraxanthone in DMSO (white square) or of solvent (DMSO in saline) alone ... Ster et al, submitted) Actually, it was demonstrated that transplantation of this cell line JOK-1 into SCID mice led to the establishment of a CLL model, allowing the evaluation of the antileukemic...

Ngày tải lên: 10/08/2014, 22:21

... Enhancement of goblet cell hyperplasia and airway hyperresponsiveness by salbutamol in a rat model of atopic asthma Thorax 2001, 56:19-24 30 Tamaoki J, Tagaya E, Kawatani K, Nakata J, Endo Y, Nagai A: Airway ... corticosteroids in asthma The Journal of allergy and clinical immunology 1998, 102:531-538 29 Kamachi A, Munakata M, Nasuhara Y, Nishimura M, Ohtsuka Y, Amishima M, Takahashi T, Homma Y, Kawakami Y: Enhancement ... this model because it has a number of practical advantages, has been well characterized, and exhibits at least some of the features thought to be central to human asthma [6] And while there are a...

Ngày tải lên: 12/08/2014, 11:20

... these measures were then analyzed as raw data [19] According to data distribution and number of groups, a parametric (analysis of variance [ANOVA], t test) or non-parametric (Kruskal-Wallis, MannWhitney) ... the efficacy of a maintenance dose of TNFK in treating the late flare of arthritis (Figure 3b, c) Histological inflammation and destruction were assessed at 45 weeks of age (Table 1) All of the ... incidence of inflammation/destruction as evaluated by histology is the number of mice with a score of inflammation/destruction of at least 0.25 Results are given as mean ± standard error of the mean aP...

Ngày tải lên: 12/08/2014, 11:22

... a priory, mechanistic models in simulations of disease spread and combining them with an a posteriori, statistical approach: Often data is available that allows fitting a simulation model that ... TS is the sole author of this paper 12 Additional material Additional file Contact Data This additional file contains the processed empirical contact data that was used for calculating the results ... to the mathematical theory of epidemics Proc R Soc Lond 1927, 11 5A: 700-721 Auranen K, Eichner M, Leino T, Takala AK, Makela PH, Takala T: Modelling transmission, immunity and disease of Haemophilus...

Ngày tải lên: 13/08/2014, 16:20

... 24:1009-1034 43 Takimoto M, Hamada A, Tomoda A, Ohdo S, Ohmura T, Sakato H, Kawatani J, Jodoi T, Nakagawa H, Terazono H, Koyanagi S, Higuchi S, Kimura M, Tukikawa H, Irie S, Saito H, Miike T: Daily expression ... streptavidin phycoerythrin and scanned on the Agilent Gene Array Scanner™ (Agilent Technologies) Analysis of microarray data We compiled a database that includes 38 Focus GeneChip® microarrays (Affymetrix) ... synthesis pathways Two additional pathways, a lipid metabolism pathway, and a cellular assembly and organization pathway, included, respectively, 71- and 68-TIR gene matches The top matching module...

Ngày tải lên: 13/08/2014, 21:21

... facial bones and cranial bones as well as intracranial injuries are evaluated based on the tolerance limits of the biomechanical parameters General trend of maximum intracranial biomechanical parameters ... to all of my colleagues and my best friends Arpan Gupta, Guo Shifeng, Zhu Jianhua, Zhuang Han, Liu Yilin, Kyrin Liong, Saeid Arabnejad Khanooki, Shahrokh Sepehri Rahnama, Ahmadali Tahmasebimoradi, ... wave propagation paths in the facial skeleton and the intracranial brain presented to study the association of the traumatic brain injury (TBI) with the facial trauma sequences Fractures of facial...

Ngày tải lên: 10/09/2015, 09:04

Ngày tải lên: 05/10/2012, 08:23

... sequence of rat TLR4 (GenBank accession NM_019178) were: 5’-CUACCAACAGAGAGGAUAU-3” (siRNA1), 5’-GUCUCAGAUAUCUAGAUCU-3’ (siRNA2), 5’-GAGCCGGAAAGUUAUUGUG-3’ (siRNA3) All siRNAs were chemically synthesized ... system (Amersham Pharmacia Biotech, Uppsala, Sweden) Histone3 (Sigma, St Louis, MO, USA, 1:500) was used as an internal control Statistical analyses Data are expressed as mean ± standard deviation ... once daily for days, starting from day before CCI surgery Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant heat and paw withdrawal threshold (PWT)...

Ngày tải lên: 25/10/2012, 11:48

Ngày tải lên: 21/12/2013, 03:20

... Systems Modelling Database and can be accessed at http://jjj.biochem.sun.ac.za/ database/curien/index.html free of charge Fig Phser branch-point in the aspartate-derived amino acid biosynthetic pathway ... can be calculated as follows: ELISA assays were carried out using rabbit antibodies raised against the recombinant proteins [12,14] and puried proteins as standards We measured that an extract ... with KALEIDAGRAPH (Abelbeck Software, Reading, PA, USA) A series of constant or changing values were generated for the dierent input variables and the calculations were done using the appropriate...

Ngày tải lên: 20/02/2014, 02:21

... what the humans in the corpus One difficulty with evaluating a model such as ours is that human speakers differ markedly in their own backchanneling behaviour As Ward and Tsukahara (2000) remark, ... carrying out a human evaluation of these models it would be hard to decide between a Three Trigram model with a pause threshold of 600ms and a Ten Trigram model with a threshold of 900ms Evaluation ... 90% or above are not uncommon for tasks such as part -of- speech tagging and statistical parsing, this can be at least partly explained by the fact that humans vary widely in how many of their opportunities...

Ngày tải lên: 22/02/2014, 02:20