... vectors generate efficient knockdown of target proteins To examine the efficiency of generation of RNAi for pSM155 and pSM30, we examined the knockdown of expression of firefly Luciferase (Luc) HeLa cells ... efficiency for the downstream ORF in unprocessed pri-miRNAs However, expression of the marker (regardless of the placement location) could only occur at the expense of failure of cleavage of primiRNA ... Banno for the PLD2 antibody, Dr Greg Hannon for the pSM2 vector, Dr Roger Y Tsien for the pRSET-B-mCherry, and Dr Jen-Chih Hsieh for the pRK-human IgG construct We also thank Dr Jian Cao for allowing...
Ngày tải lên: 07/03/2014, 11:20
... Isolating transformers and safety isolating transformers Specification for transformers for reduced system voltage BS EN 60742 Isolating transformers, and safety isolating transformers BS 3676/BS ... consisting of a mixture with air of flammable substances in the form of a gas, vapour or mist is not likely to occur in normal operation but, if it does occur, will persist for a short period only For ... in the form of a cloud of combustible dust is present continuously or for long periods or frequently Zone 21 (ATEX category 2D {Dust}) A place in which an explosive atmosphere in the form of a...
Ngày tải lên: 23/03/2014, 08:20
Design of hydraulic systems for lift truck
... Mechanics of the Fluids, Hydraulic Circuits and Components, Management of Quality, Project Management, Design for Excellence and Professional Communication Some of these courses, in most of the ... only rigidity of the fluid but also rigidity of the whole system Bulk Modulus of the fluid is one of the main factors that determine the rigidity of the system There are a number of cases when ... pressure The most convenient form of this formula for calculating the input power on the pump shaft is: P= pQ [kW ] 60η P Where the units are: 2.25 Design of Hydraulic Systems for Lift Trucks ⎡ l ⎤...
Ngày tải lên: 23/03/2014, 11:37
Báo cáo hóa học: " Exploring the bases for a mixed reality stroke rehabilitation system, Part II: Design of Interactive Feedback for upper limb rehabilitation" doc
... Quantification of kinematic attributes within the representation of action provides detailed information on movement performance for generation of the interactive media-based feedback Design of Interactive ... participant to observe movement of his end-point by monitoring formation of the image, and when needed, apply this information for online control of his movement to adjust for vertical or horizontal ... 3.offline terminal 1.explicit 2.implicit 3.explicit 1.online control 2.feedforward 3.feedforward Speed Rhythm of music audio concurrent continuous implicit feedforward Velocity Profile Image formation...
Ngày tải lên: 19/06/2014, 08:20
Báo cáo hóa học: " Multiple-Clock-Cycle Architecture for the VLSI Design of a System for Time-Frequency Analysis" doc
... Editor of the IEEE Transactions on Image Processing He is a Member of the Yugoslav Engineering Academy, and a Member of the National Academy of Science and Art of Montenegro (CANU) Professor ... The kth channel, one of the N channels (obtained for k = 0, 1, , N − 1), is described by (3)-(4) Note that it will consist of two identical sub-channels used for processing of STFTRe (n, k) and ... and For that purpose, the SM block of the proposed architecture and the second input of the output adder in the SM block must be shared (by introducing two-input multiplexors) for realization of...
Ngày tải lên: 22/06/2014, 23:20
shear reinforcement for slabs
... Appendix A CHAPTER 2—ROLE OF SHEAR REINFORCEMENT Shear reinforcement is required to intercept shear cracks and prevent them from widening The intersection of shear reinforcement and cracks can ... per in.2 For prestressed slabs, see Chapter 3.3.1 Shear strength without shear reinforcement For nonprestressed slabs, the shear strength of concrete at a critical SHEAR REINFORCEMENT FOR SLABS ... allow the use of shear studs for flat slabs (Fig 1.2) Design rules have been presented9 for application of British Standard BS 8110 to stud design for slabs Various SHEAR REINFORCEMENT FOR SLABS...
Ngày tải lên: 24/10/2014, 22:00
Analysis of Closed-Loop Performance and Frequency-Domain Design of Compensating Filters for Sliding Mode Control Systems
... involvement of the filtering hypothesis Let us formulate that as the following theorem, the proof of which is given as the formulas above Theorem For the existence of a periodic motion of frequency ... sign of the high-frequency segments of the LPRS (Lemma 2) The condition of non-existence of periodic motion of finite frequency in a SM system (chattering) is therefore the condition of absence of ... of J(ω) contains information about gain kn , and the imaginary part of J(ω) comprises the condition of the switching of the relay and, consequently, contains information about the frequency of...
Ngày tải lên: 01/07/2015, 09:37
Characterization and design of CMOS components for microwave and millimeter wave applications
... performance because of the high mobility of the electrons However, these technologies are costly for consumer electronics Silicon-based technologies have received profound interest for reasons of ... the performance of the MOSFET remains the foremost component to represent the progress of the process In terms of MOSFET application for RF circuits, there are three most important figure -of- merit ... up to tens of GHz A modified model of the “thru” standard accounting for the shunt parasitics of the interconnects is exploited to improve the conventional “thru” method For reasons of accuracy,...
Ngày tải lên: 12/09/2015, 09:42
Design of protein linkers for the controlled assembly of nanoparticles
... Illustration of the position of foreign peptide sequences inserted at the C-terminus of wild-type LacI 92 Table 5.2 Analysis of binding kinetics of wild-type LacI, LacI-STB1 and LacI-C7AC for ... conformations The protocol of using PSD for selecting inorganic-binding peptides is quite similar to that of using CSD (Figure 2.2) However, the platform of PSD, phage, is simpler than that of ... Schematic illustration of the putative structure of STB1-P layer formed on SiO2 or TiO2 at pH 9.9 (lengths not to scale) 53 Figure 3.8 Time course of Δf and ΔD for the binding of STB1-P or W-M13...
Ngày tải lên: 14/09/2015, 14:08
Design of viral vectors for improved gene delivery
... buffer for isolation of nuclear extract for Electromobility shift assay (EMSA) (Wu et al., 2001) 2.1.43 Buffer A for isolation of nuclear extract (Wu et al., 2001) 2.1.44 Buffer C for isolation of ... my family and friends for their support and encouragement i TABLE OF CONTENTS Acknowledgements Table of Contents Summary List of Tables List of Figures Abbreviations List of Publications i ii ... 5.2.10 Amplification of phage clones 5.2.11 Titering of phage 5.2.12 In vivo targeting of MG11 phage to tumor xenograft 5.2.13 Formation of peptide/DNA complexes 5.2.14 Transfection of tumor cell lines...
Ngày tải lên: 16/09/2015, 15:54
Design of artificial microenvironment for cord blood CD34+ cells expansion ex vivo
... lots of gratitude for having me shown this way of research I also would like to thank the helpful discussion of Professor Ian McNiece, Professor Hai-Quan Mao, and Dr Chai Chou My colleagues of ... product for transplantation 2.2.1 Selection of candidate cells for expansion There are a number of issues related to the selection of a candidate stem population for ex vivo expansion Before determining ... cohesiveness for the marrow structure There is a growing body of evidence indicating that ECM is important for the regulation of hematopoiesis For example glucosaminoglycan side chains of heparan...
Ngày tải lên: 04/10/2015, 15:44
Design of LCL filters for the back to back converter in a doubly fed induction generator
... voltage of gird, Im is the peak current of grid, ωB is the angular frequency of grid voltage Low impedance of XC3 means large capacitor value of C3, which will result in large reactive power For ... values of L1, L2, C3, and R3 can be deduced 1) The total value of inductance should limit current ripple of I1 to 15%-25% of rated current [3] In Fig 1, the current I1 depends on the impedance of ... current ripple of I1g, a minimum value of 0.65mH is required for inductance L1g L1g should be less than 2.2mH according to (5) Here, 1.0mH is adopted for L1g 2) The maximum value of capacitor C3g...
Ngày tải lên: 25/08/2016, 19:15
Design of piping systems for the food processing industry
... Stainless steel of varying quality is the most used material in the food industry for the construction of machines and processing equipment This is due to the ability of steel of forming a chromium ... much means: • • • costs for the purchase of an elbow costs for the installation increased energy costs for the entire lifetime of the plant due to pressure loss It is therefore important to think ... (SEM) for the inspection and photographic documentation of the surface finish (topography) Roughness measurements for the documentation of Ra and Rz values and the recording of surface profiles,...
Ngày tải lên: 11/11/2016, 17:21
Part 9: Code of practice for stressed skin design pptx
... of practice for design of simple and continuous composite beams; — Part 4: Code of practice for design of composite slabs with profiled steel sheeting; — Part 5: Code of practice for design of ... 1.3.21 shear stiffness the shear load per unit in-plane displacement of a shear panel or diaphragm NOTE Shear stiffness is the reciprocal of shear flexibility 1.3.22 sheeting generic name for roof ... practice for fire resistant design; — Part 9: Code of practice for stressed skin design This Part of BS 5950 gives recommendations for the use of profiled steel sheeting as “stressed skin” shear...
Ngày tải lên: 08/07/2014, 22:20
Luận văn tiếng anh earthquake response analysis and resistant design of moderately ductile reinforced concrete shear walls considering higher mode effects
... demand for planar (rectangular cross section) shear walls However, the related prediction underestimated approximately by 70% base shear force demand for U shape shear walls Moreover, shear force ... shape) RC shear walls in the context of an existing building considering the interactions between different shear walls j The investigation of the efficiency of the proposed shear force magnification ... plastic deformations at the base of the walls However, the related shear demand prediction underestimated the base shear force computed from 3D nonlinear dynamic analyses for U-shaped shear walls...
Ngày tải lên: 17/11/2016, 14:44
Performance of Modern Techniques for Rating Model Design
... parametric form for the ratio of two class-conditional distributions instead of specifying a parametric form of the class-conditional distributions themselves By denoting the distribution of the ... considered as the benchmark for clever guessing, having a prior probability of 35.55% Fig.2: Histogram and Density plots of the Data Performance of modern techniques for rating model design The ... Quantization 6.4 SelfOrganized Maps Conclusions and Further Research Performance of modern techniques for rating model design Introduction Credit risk forecasting is one of the leading topics...
Ngày tải lên: 16/04/2013, 20:00
Novel design of a compacted micro-structured air-breathing PEM fuel cell as a power source for mobile phones
... permeability for the gas phase (KP ) The relative permeability accounts for the reduction in pore space available for one phase due to the existence of the second phase [7] The momentum equation for ... account for the magnitude of phase change inside the GDL, expressions are required to relate the level of over- and undersaturation as well as the amount of liquid water present to the amount of water ... concentration of oxygen at the end of the cell width of the electrode has already reached low values It becomes clear that the diffusion of the oxygen towards the catalyst layer is the main impediment for...
Ngày tải lên: 05/09/2013, 14:58
An Introduction to Intelligent and Autonomous Control-Chapter 4:Design of Structure-Based Hierarchies for Distributed Intelligent Control
Ngày tải lên: 17/10/2013, 19:15
Tài liệu Design of Sheet Pile Walls doc
... responsibility for design and installation of safe and economical sheet pile walls used as retaining walls or floodwalls FOR THE COMMANDER: WILLIAM D BROWN Colonel, Corps of Engineers Chief of Staff ... purpose of this manual is to provide guidance for the safe design and economical construction of sheet pile retaining walls and floodwalls This manual does not prohibit the use of other methods of ... penetration, and design forces for cantilever or single anchored walls assuming limiting states in the wall/soil system w Factor of safety: (1) Factor of safety for rotational failure of the entire wall/soil...
Ngày tải lên: 13/12/2013, 11:15