6 tissue engineering of articular cartilage with mechanical preconditioning
Transplantation and improvement of mouse embryo progenitor derived insulin producing cells for type 1 diabetes therapy
... Introduction adult human constituting around 1-2% of the mass of the pancreas Besides -cells, the islet of Langerhans is composed of a small quantity (20-40%) of other endocrine cells (i.e -, -, PP- and ... techniques……………………………………… 15 1.2.5.1 Ratinale of encapsulation of cells……………………………… 15 1.2.5.2 Principle and application of encapsulation……………………… 16 1.3 Roles of Maf A in -cell development and function.…………………… ... 34 2.2.3 Transplantation and washing-out of capsules………………… …… 35 2.2.4 Measurement of insulin secretion of microcapsules ………… …… 36 2.2.5 Measurement of cell number in microcapsule………………… ……...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... expression of these genes in cells treated with or without ECM We then quantified the gene expression of insulin and Glut2 RT-PCR revealed adding FN or LAM increased the expression of Insulin ... increased the percentage of proinsulin and insulin expressing cells with the maximum effect seen in cells treated with LAM (Figure 2) These data are consistent with the mRNA levels of Insulin and Glut2 ... bromide with the housekeeping gene (b-actin) as a control For real-time PCR, the amplification was carried out in a total volume of 25 μL containing 0.5 μM of each primer, mM MgCl , 12.5 μL of LightCycler™-FastStart...
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Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx
... infected with single-cycle reporter virus pseudotyped with VSV-G for a time period sufficient to allow integration of the viral genetic material within host genome (i.e 48 hours) [37] The use of such ... hours of treatment with LPS (unpublished data) Therefore, it is not surprising to obtain different results with the two conditions in regard to DCmediated trans-infection of CD4+ T cells with ... efficiency of HIV-1 transmission is enhanced following maturation of DCs [8,16,54,63] Considering the up-regulatory effect of NF-κB with regard to HIV-1 transcription and the potent induction of this...
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Mypt1 mediated spatial positioning of bmp2 producing cells is essential for liver organogenesis
... hypoglycemia and failure of expression of genes associated with hepatocyte activities Disruption of the formation of bile canaliculi, a consequence of the failure of hepatocyte expression of cell junction ... source of strength to move forward i Table of Contents Acknowledgement i Table of Contents ii Summary vi List of Abbreviations viii List of ... small liver with relatively normal morphology 86 Figure 3-8 Representatives of mutants with liverless or small liver and abnormal morphology 88 Figure 3-9 One representative of mutants with the...
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Báo cáo y học: " Production of infectious human immunodeficiency virus type 1 does not require depletion of APOBEC3G from virus-producing cells" pdf
... proteasome-dependent degradation of the protein and requires a direct interaction of Vif with APOBEC3G [3,68] Confocal microscopic analysis of HeLa cells transiently transfected with Vif and APOBEC3G expression ... co-immunoprecipitation of Vif and APOBEC3G from cell extracts is not necessarily an indication of the existence of stable intracellular complexes Quite to the contrary, Marin et al reported a profound effect of ... visualized with Texas red-conjugated antibodies (red) Areas of overlap appear as yellow viruses produced in the presence of various ratios of Vif and APOBEC3G To allow a direct comparison with the...
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Investigation of the roles of two rac1 effectors phosphatidylinositol 5 kinase 1a and p21 activated kinase 1 in insulin secreting cells
... SUBMITTED FOR THE DEGREE OF PHILOSOPHY OF DOCTORATE DEPARTMENT OF BIOCHEMISTRY NATIONAL UNIVERISTY OF MEDICAL INSTITUTES NATIONAL UNIVERSITY OF SINGAPORE 2008 ACKNOWLEDGEMENTS First of all, I would like ... substitution mutant of PIP5K-Iα blocked Rac mediated actin assembly in platelets (159) As for other isoforms of type I PIP5Ks, overexpression of either β or γ isoform increased the number of short actin ... (135;136;150)) Rac interacts with type I PIP5Ks directly, which is independent of GTP (147;151) The C terminus of Rac is necessary and sufficient for its binding with PIP5K The products of PIP5K are involved...
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ROLES OF HUNTINGTIN ASSOCIATED PROTEIN 1 IN INSULIN SECRETING CELLS
... secretion are 16 quite complex because of the implication of a series of intracellular trafficking processes with participation of many partners Although some of the events and cascades in the underlying ... survival A sequence of 36 or more polyQ alters the interaction of Huntingtin with other proteins and accelerates the decay of some neurons, which leads to the pathogenesis of Huntington's disease ... are two isoforms in rat, HAP1 isoform A (HAP1A) and HAP1 isoform B (HAP1B), which differ at their C-terminals One human HAP1 isoform has been characterized that shares great similarity with rat...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... form of IGFBP-rP1 A B C Fig Effects of matriptase siRNAs on IGFBP-rP1 processing in culture of OVISE cells OVISE cells at 50–60% confluence in 60 mm culture dishes were transfected with 200 pmol of ... 200 pmol of each of three siRNAs (si973, si1513 and si2578) using Lipofectamine 2000 reagent As negative controls, the cells were treated with a scrambled RNA (sc) or with the lipofectamine reagent ... conditions and the types of the cell lines used are described in ‘Experimental procedures’ Fig Effects of proteinase inhibitors on processing of IGFBP-rP1 in culture of OVISE cells OVISE ovarian...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
... constructed that was extended with the C-terminal 274 amino acids of Mls1p (out of a total of 554), including the terminal SKL Expression of this GFP-Mls1p was compared to that of a control GFP extended ... compartmentalization of the yeast glyoxylate cycle into peroxisomes has been put into question in light of chronicled observations of growth of S cerevisiae pex mutants devoid of functional peroxisomes ... revealed a protein band with an Ó FEBS 2002 apparent molecular mass of 38 000, which corresponded to the deduced size of the His6-Mls1p truncation (not shown) A fraction of a puri®ed His6-Mls1p...
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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
... of tyrosinephosphorylation of the insulin receptor or of IRS1, or of phosphorylation of ERK1 ⁄ 2, was not significantly different in either group of diabetics compared with the nondiabetic controls ... 2.3-fold Patients with type diabetes We next isolated adipocytes from a group of female and a group of male patients with type diabetes and examined the insulin responsiveness of the cells after ... (o ⁄ n) (average of cells from two different subjects) (B) Cells obtained after surgery were incubated overnight, treated with or without collagenase for 15 and then with or without 100 nM insulin...
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Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx
... af®nity compared with native IGF-II (Fig 2B,C and Table 1) However, replacement of Tyr27 of IGF-II with Leu results in comprehensive loss of af®nity for IGF1R [25] The af®nities of Leu27-IGF-II ... were inactivated with 35 lL M ethanolamine/HCl, pH 8.5 A sensor surface with 8±10 000 response units of coupled rhIGF1R would routinely result in a response of 100 response units with 200 nM IGF-I ... absence of IGF-I, IGF-II or mutant IGF analogues at the indicated concentrations (A) Eects of IGF-I analogues that bind IGF-binding proteins with low anity; (B) eects of IGF-I analogues with...
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Báo cáo Y học: Loss-of-function variants of the human melanocortin-1 receptor gene in melanoma cells define structural determinants of receptor function doc
... an impairment of the normal function of the aMSH/MC1R system as a result of loss -of- function mutations in the MC1R gene, might lead to an increased risk of melanoma In keeping with this view, ... ones of malignant melanocytes of defined MC1R genotype Our results show that loss -of- function, but not gain -of- function, mutations of MC1R are indeed frequent in HMC lines, and that impairment of ... reactions were performed with 30 ng of template DNA, 0.5 lg of each corresponding primer, 200 lM each dNTP and 2.5 U of the proofreading Pfu polymerase Thirty rounds of amplification were carried...
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Báo cáo khoa học: Colocalization of insulin receptor and insulin receptor substrate-1 to caveolae in primary human adipocytes Cholesterol depletion blocks insulin signalling for metabolic and mitogenic control doc
... Determination of glucose transport Glucose transport was determined as uptake of 2-deoxy3 D-[1- H]glucose [36] after transfer of cells to medium without glucose Cells were treated with or without insulin ... Membrane area with single caveolae, (B) a cluster of caveolae Images contrast inverted electron microscopy, the inside of the plasma membrane of a human fat cell was covered with large numbers of caveolae ... previously shown that reduction of the amount of cholesterol in the plasma membrane with b-cyclodextrin destroys the structural integrity of caveolae [27] Depletion of cholesterol made the human...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... in the presence of core was the result of an overloading of the cellular protein-synthesis machinery and of a shortage of its components Finally, we examined the possible effect of core+1 ⁄ S–myc ... verify the specificity of the effect of core on core+1 ⁄ S expression, Huh-7 cells were transfected with the vector expressing core+1 ⁄ S–myc (pHPI-1496) and with varying amounts of a plasmid expressing ... (b-gal), instead of core (Fig 2A) Also, Huh-7 cells were transfected with a constant amount of b-gal-expressing plasmid, instead of the core+1 ⁄ S–myc vector, and increasing amounts of the core-expressing...
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báo cáo hóa học:" Three-dimensional growth as multicellular spheroid activates the proangiogenic phenotype of colorectal carcinoma cells via LFA-1-dependent VEGF: implications on hepatic micrometastasis" docx
... 18th hour of incubation of monolayer- and 3D-spheroid-cultured CT26 cells For both culture conditions, the concentration of VEGF was expressed as a function of the total number Page of 12 (page ... disrupted with PBS-EDTA (4 mM, 10 min), stained with trypan blue for assessment of cell viability and added to 24-well-plate cultured HSE cells and, 30 minutes later, wells were washed three times with ... Statistical analysis was performed by SPPS statistical software for Microsoft Windows, release 6.0 (Professional Statistic, Chicago, IL) Homogeneity of the variance was tested using the Levene test...
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Báo cáo hóa học: " The emerging role of insulin-like growth factor 1 receptor (IGF1r) in gastrointestinal stromal tumors (GISTs)" pdf
... efficacy of different IGF1Rinhibitors and the effect of the combination with standard front line therapies [52] These analyses are particularly necessary to confirm the putative oncogenic role of IGF1R ... unsupervised analysis of gene expression profiling in our patients merged with a data set from a gastric GIST showed that IGF1R was up-regulated in two young patients (< 30 years old) with both WT disease ... SNPs array analysis of the genomic copy number showed that neither of the young patients had tumors with IGF1R amplification More recently, Janeway and colleagues studied IGF1R with WB, SNP and...
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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... depletion with anti-asialo GM1 did not decrease the duration of survival of HSV-1 infected scid mice (Fig 6B) Rather, treatment with control IgG or anti-asialo GM1 increased the duration of survival of ... t-test) B Duration of survival of HSV-1 infected BALB/c mice and scid mice treated with PBS, control IgG or anti-asialo GM (n = per group) or CyP and were inoculated with × 105 pfu/eye of HSV1 strain ... treated with cyclophosphamide d The results of Experiment are presented in Figure e The results of Experiment are presented in Figure f Mean ± SEM days of survival after ocular HSV-1 inoculation of...
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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot
... depletion with anti-asialo GM1 did not decrease the duration of survival of HSV-1 infected scid mice (Fig 6B) Rather, treatment with control IgG or anti-asialo GM1 increased the duration of survival of ... t-test) B Duration of survival of HSV-1 infected BALB/c mice and scid mice treated with PBS, control IgG or anti-asialo GM (n = per group) or CyP and were inoculated with × 105 pfu/eye of HSV1 strain ... treated with cyclophosphamide d The results of Experiment are presented in Figure e The results of Experiment are presented in Figure f Mean ± SEM days of survival after ocular HSV-1 inoculation of...
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Báo cáo y học: "Pharmacological disruption of insulin-like growth factor 1 binding to IGF-binding proteins restores anabolic responses in human osteoarthritic chondrocytes" ppt
... correlated with the severity of the disease [26] The molar concentration of IGFBP-3 in the synovial fluid of OA patients was higher than that of IGF-1, leading to a molar ratio of free to bound IGF-1 of ... proteins within cartilage (Fig 7) No further displacement was observed with higher IGF-1 R398 The loss of anabolic responses of chondrocytes to IGF-1 is one of the reasons why diseased cartilage ... inhibitory concentration (IC50) of 0.1 nM and the displacement curve obtained with NBI-31772 was parallel to that of IGF-1, with an IC50 of nM Western ligand blot analysis of IGFBPs Chondrocytes were...
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Báo cáo y học: "IFN-gamma regulation of ICAM-1 receptors in bronchial epithelial cells: soluble ICAM–1 release inhibits human rhinovirus infectio" pptx
... Page of 16 (page number not for citation purposes) Journal of Inflammation 2008, 5:8 IFN-γ treatment of NHBE cells To determine effect of the Th-1 cytokine, IFN-γ, on expression of ICAM-1 isoforms ... (min) Effect of3 Figure HRV-14 on STAT-1 levels in NHBE cells at 0–90 of infection with HRV-14 (TCID50 102.5) Effect of HRV-14 on STAT-1 levels in NHBE cells at 0–90 of infection with HRV-14 (TCID50 ... 90 mins) Effect of IFN-γ cell preconditioning (1 ng/ml for 24 expression with NHBE cells over the study period Effect of IFN-γ cell preconditioning (1 ng/ml for 24 h) singly and with HRV-14 or...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose