5 mesenchymal stem cells in cartilage regeneration

... as a coordinate partner to insulin by reducing food intake, slowing gastric empty and inhibiting digestive secretion in short term Due to the release of insulin, C-peptide and amylin, -cells play ... reported that infection of liver with recombinant adenovirus expressing Pdx-1 could induce liver cells into insulin producing cells, so-called lineage reprogramming (50) Additionally, transfecting rodent ... bio-processing from its precursors, preproinsulin and proinsulin, which ensures the production of sufficient amounts of mature insulin in the -cell The formation of insulin from the proinsulin precursor...

Ngày tải lên: 11/09/2015, 09:58

... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... Immunohistochemistry (IHC) in stage IV cells further revealed adding ECM increased the percentage of proinsulin and insulin expressing cells with the maximum effect seen in cells treated with LAM ... doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK Journal of Biomedical...

Ngày tải lên: 10/08/2014, 05:21

... HIV-1-binding protein that enhances trans-infection of T cells Cell 2000, 100:587-597 Granelli-Piperno A, Finkel V, Delgado E, Steinman RM: Virus replication begins in dendritic cells during the ... viral material and productive infection [40,41] Alternatively, incoming virions can either remain in an infectious state within intracellular vesicles or be associated in membrane protrusions and ... levels of proinflammatory cytokines (e.g IL-1β and IL-8) and these cytokines induce the secretion of other proinflammatory cytokines or recruit other immune cells, thus possibly increasing the number...

Ngày tải lên: 12/08/2014, 23:20

... concentrating signaling molecules or by mediating intracellular signal through interaction with integrins β1-integrin is a component of receptor for ECM proteins laminins and collagens The β1-integrin ... supplied by the portal vein that drains the blood from the intestinal system (including the pancreas and the spleen), rich in nutrients and poor in oxygen The remaining blood supply (about 25%) ... the terminal branches of the hepatic portal vein and hepatic artery coalesces into sinusoids in the liver and drains into the central vein in each lobule (Figure 1-3A) The hepatic vein collects...

Ngày tải lên: 11/09/2015, 16:06

... and APOBEC3G in the cytoplasm apparent by the yellow staining in panels C & F of figure Interestingly, a significant number of cells in this experiment were single-positive expressing either APOBEC3G ... protein Frequently, epitopes are masked because of the folding properties of a protein in vivo or because of pre-existing protein-protein interactions that may compete for antibody binding To ... used for the infection of LuSIV indicator cells Relative virus infectivity was determined by comparing the Tat-dependent expression of luciferase in the target cells (Fig 7) Interestingly, viruses...

Ngày tải lên: 13/08/2014, 13:20

... organization in INS-1 cells 110 4.1.2 Role of PIP5K-Iα in insulin secretion in INS-1 cells 111 4.1.3 Implication of PIP5K-Iα in PIP2 production in INS-1 cells 115 4.1.4 Role of PIP5K-Iα in glucose ... nor induced apoptosis in INS-1 cells .85 Fig 15 Knockdown of PAK1 in INS-1 cells 87 Fig 16 F-actin structure staining (A) and measurement of F-actin content (B) in INS-1 cells ... involved in mediating the action of Rac1 on actin cytoskeleton remodeling In this thesis study, their potential roles in insulin secretion and cell survival were investigated in INS-1 cell line,...

Ngày tải lên: 14/09/2015, 14:08

... expressed in pancreatic islet β -cells In my project, I knocked down the HAP1 expression by RNAi technique in INS-1 cells (a insulin secreting cell line from rat insulinoma) In insulin secretion ... and insulin HAP1 is also expressed in INS-1 cell line, a commonly-used insulin-secreting cell line from rat insulinoma Western blotting further confirms that there are two HAP1 isoforms in INS-1 ... of HAP1 in insulin-secreting cells after knockdown of its expression in INS-1 cell line The findings of HAP1 role in INS-1 cell may shed light on the understanding of complicated signaling pathways...

Ngày tải lên: 12/10/2015, 17:35

... follows: aprotinin, leupeptin, pepstatin A, AEBSF and proteinase inhibitor mixture that contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A from Wako Pure Chemical Industries (Osaka, ... (N-(R)(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-l3-(2¢-naphthyl)alaninyl-l-alanine 2-aminoethyl amide; TAPI-1) did not affect the processing A commercially available proteinase inhibitor mixture containing AEBSF, aprotinin and some other inhibitors inhibited ... serine proteinases secreted by various cell lines Trypsin-type serine proteinases present in the conditioned media of the nine cell lines were analyzed by gelatin zymography (Fig 3A) To eliminate...

Ngày tải lên: 19/02/2014, 07:20

... compartment in cells grown on glucose, Mls1p was selectively retained in the cytosol of cells propagated on ethanol Interestingly, the C-termini of both Mls1p and Aat2p contain acidic amino-acid ... strain KM11 YEp352-MLS1DSKL was constructed by inserting a 2.3-kb SalI fragment containing the complete MLS1 gene into this multicopy vector, and replacing parts of the coding region with the single-strand ... imported into the peroxisomes of wild-type cells, but to remain cytosolic in pex mutant cells devoid of functional peroxisomes [22,31] The results demonstrated that living yeast cells expressing either...

Ngày tải lên: 22/02/2014, 04:20

... postoperative insulin resistance Insulin resistance in type diabetes Tyrosine phosphorylation of the insulin receptor increased over the same concentration range of insulin in cells from patients ... protease and protein phosphatase inhibitors, frozen within 10 s, and thawed in boiling water to minimize postincubation signalling modifications in the cells and protein modifications during immunoprecipitation ... sensitivity to insulin was invariably improved The fact that even minor surgery produces insulin resistance [8] indicates that it is difficult to obtain control tissue to study traumainduced insulin resistance,...

Ngày tải lên: 07/03/2014, 16:20

... IGF2R-binding site, while the B domain and the ®rst A domain helix contain the major determinants for IGFBP binding In this study we show, using IGF-I, IGF-II and 14 IGF analogues, that potency in ... rhIGF1R binding by IGF-I, IGF-II and 14 analogues using BIAcore analysis IGF1R binding by all of these analogues has not previously been compared in a single binding assay Analogues included in this ... Shc) pathways are involved in IGF-induced antiapoptotic signalling via the IGF1R in PC12 cells [20] It would be interesting in the future to determine whether analogues differ in their abilities...

Ngày tải lên: 08/03/2014, 22:20

... yellow, sulfurcontaining phaeomelanins [17] Although both types of melanins are found in different relative proportions in all skin types, the phaeomelanin/eumelanin ratio is higher in individuals with ... MC1R are indeed frequent in HMC lines, and that impairment of signaling through the cAMP cascade occurs in these cells even in the absence of mutations in the coding region of the gene Finally, ... 24 h, cells were completely serum-deprived for an additional 24 h before binding or coupling assays Radioligand binding assay Transfected cells were incubated (1 h, 37 °C) with increasing concentrations...

Ngày tải lên: 17/03/2014, 10:20

... poorly to insulin, exhibiting insulin resistance that can be overcome by increasing concentrations of circulating insulin In the majority of cases it is still not known what defect in the insulin target ... receptors After incubating cells with a submaximal concentration of insulin the insulin-induced increase in receptor autophosphorylation was comparable in the isolated caveolae fraction and in the total ... downstream signalling for enhanced glucose uptake may take place in caveolae [25] The role of caveolae in insulin signalling has, however, not been examined in human cells As animal cells in general...

Ngày tải lên: 23/03/2014, 12:20

... Both proteins show partial endoplasmic reticulum (ER) distribution in interphase cells, and in dividing cells they also localize to the microtubules of the mitotic spindle Results Intracellular ... gene in Huh-7 ⁄ T7 cells, inasmuch as RNA molecules transcribed in the cytoplasm remain uncapped and therefore can be translated only by a cap-independent mechanism In the HCV IRES-containing ... protein have been limited by the fact that its detection in mammalian cells and in the HCV infectious system is elusive This study shows that intracellular levels of the core+1 protein in mammalian...

Ngày tải lên: 30/03/2014, 03:20

... lectin binding-site expression (wheat germ and viscum album agglutinins); and negative vitamin A storage (revealed by 328 nm of UV fluorescence) Cultures of HSE cells were established and maintained ... CT26 cancer cells Nineteen days after subcutaneous injection of monolayerand 3D-cultured CT26 cells, the number of CD31-expressing cells was determined in developed tumors As shown in Figure 4, ... C: Integrin expression on colorectal tumor cells growing as monolayers, as multicellular tumor spheroids, or in nude mice Int J Cancer 1995, 61:819-25 Rainaldi G, Calcabrini A, Arancia G, Santini...

Ngày tải lên: 18/06/2014, 15:20

... of doxorubicin and vincristine against Ewing’s sarcoma cells Clin Cancer Res 2001, 7:1790-7 Martins AS, Mackintosh C, Martín DH, Campos M, Hernández T, Ordóñez JL, de Alava E: Insulin-like growth ... pathway inhibition by ADW742, alone or in combination with imatinib, doxorubicin, or vincristine, is a novel therapeutic approach in Ewing tumor Clin Cancer Res 2006, 12:3532-40 Kim WY, Jin Q, ... PDGFRA-dependent imatinib resistance [55] Glycemic derangements related to insulin-like growth factors such as the pro-IGF-IIE and insulin-like growth factor-binding proteins have been described in GISTs,...

Ngày tải lên: 18/06/2014, 16:20

... HSV-1 infection Several clinical case reports indicate that NK cells (a major potential source of IFN-γ) are essential for innate defense against HSV-1 infection in humans [1-3] Yet, NK cells ... for determination of 51Cr release from target cells Controls for the assay included × 104 cells target cells incubated alone in culture medium (spontaneous release) and target cells incubated ... KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 infection Likewise,...

Ngày tải lên: 19/06/2014, 08:20

... HSV-1 infection Several clinical case reports indicate that NK cells (a major potential source of IFN-γ) are essential for innate defense against HSV-1 infection in humans [1-3] Yet, NK cells ... for determination of 51Cr release from target cells Controls for the assay included × 104 cells target cells incubated alone in culture medium (spontaneous release) and target cells incubated ... KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 infection Likewise,...

Ngày tải lên: 20/06/2014, 04:20

... 1996, 39:968-978 Morales TI: The insulin-like growth factor binding proteins in uncultured human cartilage Increases in insulin-like growth factor binding protein during osteoarthritis Arthritis ... small-molecule inhibitor of the binding of insulin-like growth factor (IGF)-1 to IGF-binding proteins (IGFBPs), with 125Ilabeled IGF-1 binding to cartilage labeled IGF-1 binding to cartilage Explants ... (IGF)-1 to IGF-binding proteins, on 125I-labeledinsulinbinding NBI-31772, a small-molecule inhibitor like growth factor (IGF)-1 to IGF-binding proteins, on 125I-labeled IGF-1 binding to human osteoarthritic...

Ngày tải lên: 09/08/2014, 01:23