5 bone chip model vs tissue engineered bone

... deposition and remodelling in vitro for tissue engineering and cell-based therapies Advanced Drug Delivery Reviews 2010 (In press) 28 3.1 Background Scarring is a physiological tissue response ... Background Fibrosis, a pathological accumulation of collagen in tissues, represents a major global disease burden In the field of tissue engineering, periimplantational fibrosis is a bottleneck ... simultaneously [8], and the absence of vasculature to distribute oxygen and other nutrients within a tissue- engineered device is a major limiting factor [9] f) Prolyl hydroxylase inhibitors possess antifibrotic...

Ngày tải lên: 10/09/2015, 15:52

... of donor organs and tissues available This severe organ shortage led to the inception of Tissue Engineering Tissue Engineering is about developing new approaches to encourage tissue growth and ... produce large, vascularised tissues that can integrate with the host native tissues 2.2 Biomaterials for Tissue Engineering Biomaterials is one important component in tissue engineering and regenerative ... proteins and new tissue formation Hence, the properties of the scaffolding materials are crucial in determining the success of many tissue engineering applications The general tissue engineering...

Ngày tải lên: 11/09/2015, 09:10

... the tissue over the scaffold Fig 2.5 Five core technologies (biomaterials, cells, scaffolds, bioreactors, and medical imaging) required for tissue engineering [15] 13 Tissue engineering of bone ... feasible as scaffolds for bone tissue engineering Heath et al [24] tested the coating effect of tissue transglutaminase on the surface of PCL to enhance the biocompatibility of PCL Tissue transglutaminase ... aim of tissue engineering is to restore tissue and organ functions with minimal host rejection This arose from the need to develop an alternative method of treating patients suffering from tissue...

Ngày tải lên: 14/09/2015, 08:36

... 1.2 Tissue- Engineering Scaffolds The main goal of tissue engineering is to produce new tissue where it is needed Therefore, knowledge of the structure and functional limits of the regenerated tissue ... morphogenesis of in vitro tissue engineered cartilage J Biomed Mater Res 29, 1587–1595 38 Laurencin, C T., Attawia, M A., Elgendy, H E., and Herbert, K M (1996) Tissue engineered bone- regeneration using ... of animal models in which the intention is to regenerate tissues such as skin or bone in situ We anticipate many uses of the novel FMB technology for cell culturing, wound healing, and tissue engineering...

Ngày tải lên: 10/04/2014, 11:11

... diseased animal model [14] Despite the success of demonstrating preclincal efficacy, further utilization of DCU as a tool to evaluate target PK/PD relationships in chronic animal models [24] remains ... of Page of delivering DCU to a chronic model for preclinical PK/ PD still remains unanswered In this research, a drug surface area-based in vivo absorption model was established to evaluate the ... using a Wagner-Nelson equation-based model that was established in-house in order to assess the amount of drug absorbed (dA) as a function of time [26,27] In this model, the stomach empting time was...

Ngày tải lên: 21/06/2014, 03:20

... significant bone fill The limited ability of cancellous bone grafts to repair and/or regenerate bone and periodontal attachment involves at least three separate but distinct mechanisms: the ability of bone ... radiography, microcomputed tomography, and histology for bone tissue engineering Tissue Eng 2005, 11:1356-1367 Weinberg MA, Brai M: Laboratory animal models in periodontology J Clin Periodontol 1999, ... Miller J, Freitag S, Rueger DC, Acil Y, Terheyden H: Bone formation in the presence of platelet-rich plasma vs bone morphogenetic protein-7 Bone 2004, 34:80-90 Sigurdsson TJ, Nygaard L, Tatakis...

Ngày tải lên: 11/08/2014, 23:22

... also able to build up a micro -tissue similar to that of bone repair tissue without collagenassociated mineral formation The fabrication of a selfassembled skeletal tissue seems not to be limited ... micromass tissue renouncing any other materials Other organotypic cultures, used to develop engineered tissues other then of skeletal origin, confirm that it is feasible to create tissue substitutes ... cartilage-like or bone- like tissue However, conditions allowing cartilage formation in one species are not necessarily transposable to other species Therefore, results with animal models should be...

Ngày tải lên: 11/08/2014, 23:22

... PHIs in Bone Tissue Engineering and Regeneration 64 4.1 Introduction 65 4.2 Bone development and regeneration 65 4.2.1 Mechanisms of bone formation 65 4.2.2 Bone regeneration ... construct with host tissue 1.1.3 Current vascularization strategies for engineered tissues There is therefore a clear need for effective vascularization strategies for engineered tissues, and many ... potential applications in bone The biology of bone formation and the roles of HIF-1 in bone are reviewed in chapter 12 As HIF-1 has multiple roles in bone, PHIs are likely to affect bone cells in multiple...

Ngày tải lên: 09/09/2015, 11:11

... Collection of samples via remote control in an outdoor environment, with the following aims: - • • • - Modelling of the vehicle Identification of related control parameters Development of PID-control ... Autonomous docking at the target Autonomous collection of multiple samples with the following aims: Modelling and controlling the vehicle with the following aims: - Design of a sampling device Development ... samples Each experiment consists of typical steps like: • • • • • Development of a control process model Design and programming of a first simple control Calibration of sensors and actuators Conception...

Ngày tải lên: 05/03/2014, 15:20

... patients, consisting of WBRT (30 Gy in 10 fractions) with a SIB of 10 Gy in 10 fractions to the PTVs The cumulative dose delivered to the BM(s) was thus 40 Gy in 10 fractions The treatment plans ... survived significantly longer than those not undergoing surgery: the estimated 6-month OS was 72.0% vs 33.5%, respectively (p = 0.035) Likewise, patients with good performance status lived significantly ... survivorship (data not shown) Using traditional method of statistical analysis (i.e Cox multivariate model) controlled for major clinical prognostic factors, some EORTC-C30 or -BN20 items, such as...

Ngày tải lên: 09/08/2014, 09:20

... vivo tissue engineered respiratory epithelium in sheep Med J Malaysia 2008, 63(Suppl A):34 69 Miller C, George S, Niklason L: Developing a tissue- engineered model of the human bronchiole J Tissue ... for the in vitro development of lung tissue constructs composed of primary lung cells has been developed For example, the development of a tissueengineered model of human bronchiole consisting ... chondrogenesis Tissue Eng Part A 2009, 15:243-254 58 Tognana E, Borrione A, De Luca C, Pavesio A: Hyalograft® C: Hyaluronanbased scaffolds in tissue- engineered cartilage Cells Tissues Organs...

Ngày tải lên: 10/08/2014, 05:21

... tetherin expression levels in the relevant HIV-1 tissue target cells Since it is difficult to isolate a sufficient number of CD4+ T-cells from these tissues to directly assess endogenous tetherin ... depletion in human lymphoid tissue ex vivo J Virol 2004, 78:12689-12693 Glushakova S, Baibakov B, Margolis LB, Zimmerberg J: Infection of human tonsil histocultures: a model for HIV pathogenesis ... allows productive HIV-1 infection without exogenous stimulation and mimics infection of lymphatic tissues, one of the major sites of viral Page of 13 replication in vivo [28] To study the effect...

Ngày tải lên: 12/08/2014, 23:22

Ngày tải lên: 21/08/2014, 10:51

... Vascularised Tissue- Engineered Bone Grafts: Endothelial Progenitor Cells in Vasculogenic and Osteogenic Priming of Human Fetal Mesenchymal Stem Cells International Bone -Tissue- Engineering Congress (Bone- Tec), ... vascularised bone tissue- engineered grafts “Speak Out For Engineering” by Institution of Mechanical Engineers (ImechE, Local Heats), February 2012, Singapore Y Liu Building vascularised bone tissue- engineered ... Grafts 1.4 Bone Tissue Engineering 1.4.1 Limitations in Bone Tissue Engineering 1.5 Importance of Vascularisation 1.5.1 Vascularisation in Bone Tissue Engineering...

Ngày tải lên: 09/09/2015, 10:16

... fibrosis are two challenges in regenerative medicine and tissue engineering which can limit the survival, viability and function of implanted tissue engineered constructs and biomaterials Slow neovascularization ... cells in the interior of a tissue construct; while fibrosis may occur as a result of a foreign body reaction to encapsulate the tissue construct with fibrous collagenous tissue Prolyl 4-hydroxylase ... this approach to improve vascularization of tissue engineered constructs is also novel Coupled with anti-fibrotic properties, PHi may be a potent aid for tissue engineering, by tackling both the...

Ngày tải lên: 12/09/2015, 08:18

... from bone marrow and the surrounding tissue, the state and role of the periosteal tissue is pivotal in the formation of a fracture callus, new woven bone and finally remodeled mature lamellar bone ... Multi-functionalities of the periosteum as a tissue capable of initiating bone healing, vascularization network and a barrier membrane for soft tissue were not addressed by the models Thus, explorations into ... extent of bone formation [59] Networks of blood vessels and nerve fibers are present and penetrate the underlying bone tissue via the periosteum Apart from providing the underlying bone with...

Ngày tải lên: 14/09/2015, 08:24

... the model tissue system to be studied 1.2 Vascular Tissue Engineering Vascular tissue engineering presents an attractive model for LBL tissue engineering for several reasons Firstly, vascular tissue ... indicating that the engineered tissues were capable of re-modelling as the patients grew This success was very quickly followed up with the successful clinical application of tissue engineered bladders ... TE Chapter Introduction 1.1.1 Classical Tissue Engineering Early work on tissue engineering focused on guided tissue regeneration (also known as in situ tissue engineering), where acellular polymeric...

Ngày tải lên: 14/09/2015, 08:49

... long bone structure which comprises of cortical and trabecular bone (Biomedical Tissue Research Group, 1996) Figure 2.2 Schematic diagram showing stages of bone remodelling process (Biomedical Tissue ... bone defects For surgical procedures involving bone grafts, patients usually suffer from trauma-related injuries or bone fractures Currently, the gold standard for bone grafts is autologous bone ... oLimitations to current bone grafts as discussed propel researchers to look into the field of bone tissue engineering for an ideal bone substitute 1.1.3 Strategies in BTE Tissue engineering is...

Ngày tải lên: 09/10/2015, 11:18

... wall of bone tissue They are responsible for the maintenance of healthy bone by secreting enzymes and directing the bone mineral content They also control the calcium release from the bone tissue ... allogenic bone grafts have been used for treatment in bone defects Often, the bone repair mechanism fails as a result of magnitude, infection or other causes Autogenous bone grafts are those made of tissue ... coined as bone tissue engineering” A key component in tissue engineering for bone regeneration is the scaffold that serves as a 3D template for initial cell interactions and the formation of boneextracellular...

Ngày tải lên: 09/10/2015, 11:18