5 aberrant gene expression and dna hypo methylation

Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx

Ngày tải lên : 18/03/2014, 01:20

... sensitive, and that the pA1lacO-1 promoter and the lacI q1 gene are surrounded by a DNA fragment originating from upstream the gyrA gene and a fragment containing the N-terminal part of the gyrA gene ... dependent DNA gyrase expression The expression of the DNA gyrase genes is altered by mutations that strongly affect DNA supercoiling [6,9] If in our experiments the concentration of DNA gyrase ... containing DNA from the gyrA locus on pGYRABTS with DNA fragments taken from upstream the topA gene and a fragment containing the N-terminal part of the topA gene pGYRABTS was ®rst digested with KpnI and...

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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx

Ngày tải lên : 19/02/2014, 07:20

... oat ADC cDNA coding region [10] In the present Foreign ADC gene expression in T cruzi work we used ADC-transgenic protozoa to follow the time-course modulation of foreign gene expression and to ... to measure ADC enzymatic activities and to prepare DNA and RNA for hybridization analyses Southern and northern blot hybridization Total DNA from wild-type and transformed parasites was prepared ... the parasite genome organization Results and Discussion Expression of the oat ADC gene in T cruzi In order to study the expression of the foreign ADC gene in T cruzi epimastigotes, we transformed...

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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt

Ngày tải lên : 21/02/2014, 01:21

... activation and NAMI-A- induced down regulation of c-myc gene expression NAMI-A inhibits c-myc gene transcription and protein expression To establish whether the decrease in c-myc mRNA expression ... c-myc gene expression with superimposable magnitudes and kinetics proﬁles Figure 5C shows the dose–response effect of NAMI-A on ECV304 c-myc mRNA expression As reported for DNA synthesis and ERK1/2 ... regulates c-myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in...

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..GENE CLONING AND DNA ANALYSIS..GENE CLONING AND DNA ANALYSISAn IntroductionT.A. BROWNFaculty of Life Sciences University of Manchester ManchesterSixth EditionA John Wiley & Sons, Ltd., Publication. pdf

Ngày tải lên : 06/03/2014, 22:20

... Cloning and DNA Analysis in Biotechnology 223 13 14 15 16 Production of Protein from Cloned Genes 225 Gene Cloning and DNA Analysis in Medicine 245 Gene Cloning and DNA Analysis in Agriculture 264 Gene ... Applications of Gene Cloning and DNA Analysis in Research 163 10 11 12 Sequencing Genes and Genomes 165 Studying Gene Expression and Function 185 Studying Genomes 207 Part III The Applications of Gene Cloning ... Basic Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis are Important Vectors for Gene Cloning: Plasmids and Bacteriophages 13 Puriﬁcation of DNA from Living Cells...

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Plant physiology - Chapter 14 Gene Expression and Signal Transduction potx

Ngày tải lên : 07/03/2014, 21:20

... pathways in which one or Gene Expression and Signal Transduction (A) Tryptophan operon 5′ DNA Regulatory gene i Transcription Promoter p Operator o 3′ Gene E Gene D Gene C Gene B Gene A Transcription ... is Gene Expression and Signal Transduction The levels for control of gene expression Genome Chromatin NUCLEUS Gene amplification (rare) DNA rearrangements (rare) Chromatin decondensation DNA methylation ... initiation complex The DNA sequences themselves are Gene Expression and Signal Transduction (A) General transcription factors RNA polymerase II Gene regulatory proteins Gene X DNA GGGCGG GCCCAATCT...

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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

Ngày tải lên : 16/03/2014, 02:20

... regulating the gene expression of rate-limiting enzymes of gluconeogenesis and glycogenolysis, including phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), and by regulating ... cells, and the ensuing effect on gluconeogenesis and glucose output Our results show that, in the presence of TNFa, insulin-induced inhibition of gluconeogenesis and glucose output is attenuated and ... with TBP and b-actin antibodies and taken as nuclear and cytosolic loading controls, respectively, and also used to ascertain the purity of the nuclear and cytosolic preparations (A) Bands were...

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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot

Ngày tải lên : 16/03/2014, 22:20

... uPAR expression in colon cancer invasion and metastasis [13] To develop a potential tool for uPAR gene therapy, we used a catalytic DNA enzyme (DNAzyme) approach The DNAzymes were originally generated ... of uPAR by DNAzymes C E de Bock et al Fig DNAzyme targeting sites in uPAR cDNA Schematic representation showing the exon regions of uPAR cDNA and the regions targeted by Dz372, Dz483 and Dz720 ... northern blot, and the blot was hybridized with [32P]dCTP[aP]-labeled uPAR cDNA and 18S rDNA probes as a control Autoradiographic exposure time was 24 h (for uPAR as probe) and h (for 18S rDNA as probe)...

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GENE CLONING AND DNA ANALYSIS pptx

Ngày tải lên : 19/03/2014, 09:20

... Cloning and DNA Analysis in Biotechnology 223 13 14 15 16 Production of Protein from Cloned Genes 225 Gene Cloning and DNA Analysis in Medicine 245 Gene Cloning and DNA Analysis in Agriculture 264 Gene ... Applications of Gene Cloning and DNA Analysis in Research 163 10 11 12 Sequencing Genes and Genomes 165 Studying Gene Expression and Function 185 Studying Genomes 207 Part III The Applications of Gene Cloning ... Basic Principles of Gene Cloning and DNA Analysis 1 Why Gene Cloning and DNA Analysis are Important Vectors for Gene Cloning: Plasmids and Bacteriophages 13 Puriﬁcation of DNA from Living Cells...

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Báo cáo khoa học: Bile acids increase hepatitis B virus gene expression and inhibit interferon-a activity pot

Ngày tải lên : 22/03/2014, 21:21

... metabolism and HBV gene expression H Y Kim et al the liver, intestine, kidney and adipose tissue via the regulation of the expression and function of genes involved in bile acid synthesis, uptake and ... metabolism and HBV gene expression Fig The effects of bile acids on HBV gene expression in hepatocyte cell lines (A) Chang liver, HepG2 and Huh7 cells were transfected with the 1.3x HBV-luc construct and ... metabolism and HBV gene expression Fig The effect of AP-1 and C ⁄ EBPs on bile acids-induced HBV gene expression (A) Chang liver cells were cotransfected with AP-1-luc or 3xC ⁄ EBP-luc construct and...

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Báo cáo khoa học: Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes docx

Ngày tải lên : 28/03/2014, 22:21

... SREBP target gene, and that the transcription of SREBP-1a gene is predominantly regulated by the general transcription factor Sp1 [11,12] We next compared the glutamineinduced gene expression of ... of SREBP gene expression is not the initial trigger for the glutamine effects If induction of the SREBP gene expression serves as the initial trigger for the glutamine effects, the gene expression ... in the induction of SREBP-1a gene expression When endogenous Sp1 expression was reduced to 20% of normal with gene- speciﬁc small interfering RNA (siRNA), the gene expression of SREBP-1a was signiﬁcantly...

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Báo cáo khoa học: Selenium affects biosilica formation in the demosponge Suberites domuncula Effect on gene expression and spicule formation pot

Ngày tải lên : 30/03/2014, 20:20

... formation complete ORF of the SDSPIaP cDNA was used; again the cDNA was inserted into the vector at the same restriction sites, NcoI and HindIII Expression and puriﬁcation procedures were the same ... (2000) Expression of silicatein and collagen genes in the marine sponge Suberites domuncula is controlled by silicate and myotrophin Eur J Biochem 267, 4878–4887 Muller WEG (2005) Spatial and temporal ... Obrecht-Pfumio S, Krol A, Thisse B & Lescure A (2003) Spatial and temporal expression patterns of selenoprotein genes during embryogenesis in zebraﬁsh Gene Expr Patterns 3, 525–532 22 Pisera A (2003) Some...

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Báo cáo khoa học: A functional polymorphism at the transcriptional initiation site in b2-glycoprotein I (apolipoprotein H) associated with reduced gene expression and lower plasma levels of b2-glycoprotein I docx

Ngày tải lên : 31/03/2014, 07:20

... [30,31] However, our in vitro mutagenesis and expression study did not link these mutations to an altered b2GPI gene expression [32] Although, in vitro mutagenesis and expression study not rule out ... mutagenesis and expression studies revealed that these mutations were not associated with altered expression or secretion of recombinant b2GPI (rb2GPI) [32] We hypothesized that the Cys306Gly and ... analyzed by OpenGene Automatic DNA Sequencer System (Visible Genetics, Suwanee, GA, USA) for mutation detection Experimental procedures The genomic DNA was isolated from buffy coats and liver tissues...

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báo cáo hóa học: " Pro-inflammatory gene expression and neurotoxic effects of activated microglia are attenuated by absence of CCAAT/enhancer binding protein b" potx

Ngày tải lên : 19/06/2014, 22:20

... proinflammatory gene expression in C/EBPb-/- mixed glial cultures Expression of pro-inflammatory (NOS2, IL-1b, IL-6 and TNFa) and anti-inflammatory (IL-4 and TGFb1) genes in C/EBPb+/+ (white bars) and C/EBPb-/- ... the pathogenesis of many neurological disorders The present study stems from this hypothesis and from the hypothesis that there is a regulatory role for C/EBPb in pro-inflammatory gene expression ... the anti-inflammatory genes IL-4 and TGFb1 Absence of C/EBPb results in significant decreases in LPS-induced expression of NOS2 and in LPS+IFNg-induced expression of NOS2 and IL-1b Results are...

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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc

Ngày tải lên : 20/06/2014, 01:20

... antiviral, IFN-stimulated genes (ISGs) As specific and sensitive markers we used the ISG56 gene which is induced both by IFNs and by virus infection [38,39] and the MxA gene which is exclusively ... cytokine-inducing viruses activated IP-10 and RANTES expression in HEK 293 cells as expected (Fig 4B, upper and lower panels) Thus, SARS-CoV induces IP-10 gene expression in Caco-2 cells, but not in ... study, and was responsible for drafting and finalizing the manuscript All authors read and approved the final manuscript Acknowledgements We thank Otto Haller for support and helpful comments, and...

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báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx

Ngày tải lên : 20/06/2014, 08:20

... and identified gene expression patterns altered during the development of chemoresistance Among the genes that are consistently elevated we identify previously known genes such as ABCB1 and genes ... generate novel drug resistant ovarian cancer cell lines independently selected for resistance to cisplatin, doxorubicin or paclitaxel, and we use gene expression profiling to identify genes and ... of gene expression changes This suggested different mechanisms of resistance for the different drugs Comparison of gene expression between sensitive and resistant lines revealed numerous genes...

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Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf

Ngày tải lên : 09/08/2014, 01:21

... of chemokines and their receptors [10–12] Cytokines and chemokines in pSS In patients with pSS the salivary and lacrimal glands are the main target organs for the immune system and, at least ... encoded by VκA2, O8/O18, L11, A17 and A27 [62] Moreover, Vλ genes of the Vλ2 and Vλ7 family were found in the Hib-antibody VL gene repertoire [62] In addition, VκA27 and Vλ2C, 2E, 2A2 or 10A were ... the hypothesis of homing and activation of specific B cells in the glands B cell malignancies and Sjögren’s syndrome In contrast to the focal sialadenitis of the minor (labial) salivary glands,...

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Báo cáo y học: "Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis" pptx

Ngày tải lên : 09/08/2014, 06:23

... 0.01) and lumican (14.6-fold; P < 0.01) In the same region there were downregulations of decorin (1.6-fold; P < 0.01) and CTGF (2.1-fold; P < 0.05), and unchanged expression of fibromodulin and ... University for her assistance with the animal handling and care 22 Maroudas AI: Balance between swelling pressure and collagen tension in normal and degenerate cartilage Nature 1976, 260:808-809 ... health and osteoarthritis Evidence for molecular heterogeneity and extensive molecular changes in disease J Clin Invest 1992, 90:2268-2277 Matyas JR, Adams ME, Huang D, Sandell LJ: Discoordinate gene...

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Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx

Ngày tải lên : 09/08/2014, 06:23

... collected and processed with GeneAmp® 5700 SDS software and given as a threshold cycle (Ct) Plasmid DNA containing the target gene sequences was used to generate standard curves A DNA standard curve ... of both MMP-13 mRNA expression and the protein to an approximately similar extent Cathepsin K gene expression and protein synthesis The levels of both the gene expression and the protein of cathepsin ... correlation exists between the mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA...

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Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx

Ngày tải lên : 09/08/2014, 14:20

... (HexB) gene was characterized by significantly higher gene expression in RA and OA SFs as compared with RA and OA SM tissue samples (Mann – Whitney rank sum test) Gex, gene expression; HGPRT, hypoxanthine ... samples HexA and HexB genes were characterized by the second strongest gene expression in all samples (Figure 1) The expression of HexA gene was approximately the same both in SFs and in SMs In ... 100% As shown in Figures 5a and 6a, TGF-β1 has significantly downregulated the expression of HexA and HexB genes, as well as of GusB and Hc-gp 39 The suppression of gene expression was more pronounced...

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Báo cáo y học: "JNK pathway is involved in the inhibition of inflammatory target gene expression and NF-kappaB activation by melittin" ppsx

Ngày tải lên : 11/08/2014, 08:22

... and COX-2 expression, and on NO and PGE2 generation To investigate whether the suppressed effect of SP600125 on the inhibitory effect of bee venom and melittin on the inflammatory gene expression, ... expression, iNOS and COX-2 expression was determined The inhibitory effect of melittin and bee venom on iNOS and COX-2 expression by LPS and SNP in Raw 264.7 cells (Fig 5A) and in synoviocytes ... melittin and bee venom on the inflammatory gene expression induced by LPS or Figure SNP JNK inhibitor suppressed inhibitory effect of melittin and bee venom on the inflammatory gene expression...

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