... and mentioned "Big Bird" during the first debate, "Big Bird" began trending on Twitter Early on, the TV network bought a "sponsored tweet" that turned up high on the list when people clicked on ... engage your communit y Find out what people expect and want from your brand As an example, Steele said the Romney campaign posts behind -the- scenes campaign trail photos Show personalit y Have fun ... third-party conservative group tweeted about ABC's Dancing With the Stars during the show Move people t o act ion Steele says, like the political campaigns, you can use Twitter to trigger transactions...
Ngày tải lên: 07/01/2014, 15:28
... identity was found with the hypothetical MTAPII from P furiosus (48%) and with the hypothetical PNP from Aquifex aeolicus (40%) Among the related enzymes isolated from various sources, SsMTAPII shows ... their thermostabilities The double mutant, in fact, shows thermal properties lower than those of the single mutant, indicating a significant role of the pair C259C261 in the stabilization of the ... shows the protective Fig (A) Effect of MTA and phosphate on the thermostability of SsMTAPII in the presence of 2% SDS The enzyme was incubated at 70 °C with 2% SDS in the absence (s) and in the...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt
... Indeed, the UV-spectrum of the recombinant 2-5A synthetase from G cydonium, enriched on the Ni-NTA column and dialyzed thereafter, had a maximum at 260 nm By contrast, the UV-spectrum of the analogously ... Based on the amino acid sequence, the calculated pI of the recombinant 2-5A synthetase from G cydonium is 9.6 [27] Therefore, the protein should be positively charged at neutral pH However, the analysis ... successfully used in the studies of the respective proteins [34,35] Applying this approach for the production of the first recombinant protein of invertebrate origin, the 2-5A synthetase from the sponge...
Ngày tải lên: 23/03/2014, 09:20
Tài liệu The UN’s Role in Nation-Building - From the Congo to Iraq pptx
... democratic regimes there For the United Nations, the comparable precursor was in the early 1960s in the newly independent Belgian Congo The Republic of the Congo failed almost from the moment of its ... conducts research and analysis for the Office of the Secretary of Defense, the Joint Staff, the Unified Commands, the defense agencies, the Department of the Navy, the U.S intelligence community, allied ... missions The United Nations was quickly able to secure xv xvi The UN’s Role in Nation -Building: From the Congo to Iraq the removal of Belgian forces Over the next three years, UN troops forced the...
Ngày tải lên: 18/02/2014, 00:20
Mental health: facing the challenges, building solutions Report from the WHO European Ministerial Conference potx
... Mental health: facing the challenges, building solutions Report from the WHO European Ministerial Conference Mental health: facing the challenges, building solutions Report from the WHO European Ministerial ... wishes The absence of the voices of users and carers from the process reflects the stigma of mental health problems, and can reinforce negative attitudes These are the challenges that the WHO ... Conclusion The key themes mentioned here are the critical issues that emerged from discussions during the Conference There is no room for complacency It is time to move from words to action, and the...
Ngày tải lên: 06/03/2014, 02:20
The History of England from the Accession of James II, Vol. 5 pptx
... and set at the bar of the Peers From that moment he was the prisoner of the Peers He had been taken back from the bar to the Tower, not by virtue of the Speaker's warrant, of which the force was ... Crown, the authority of the Parliament, and the unity of the empire Already might be discerned among the Englishry, who were now, by the help and under the protection of the mother country, the ... all the Strand, all the river were in commotion Before midnight the King's apartments, the Queen's apartments, the Wardrobe, the Treasury, the office of the Privy Council, the office of the Secretary...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar specificity in the structural family ppt
... contacts with their equivalents in the other subunit of the second dimer (Fig 4A) In the case of P21212 (TcRpiB-R5P), the four molecules in the asymmetric unit represent the tetramer The two active ... in the biological cycle of the parasite (i.e the epimastigote and the metacyclic trypomastigote in the insect vector, and the intracellular amastigote and the bloodstream trypomastigote in the ... MtRpiB [13] The difference in the behaviour of the two enzymes appears to arise from the slightly broader active site in MtRpiB as a result of the switch of the catalytic base Accordingly, the longer...
Ngày tải lên: 15/03/2014, 00:20
Báo cáo khoa học: A chloroplast RNA binding protein from stromal thylakoid membranes specifically binds to the 5¢ untranslated region of the psbA mRNA potx
... to +1157 relative to the AUG of the psbA mRNA (corresponding to the coding region of the C-terminal amino acids of D1 and the 3¢ UTR of the psbA mRNA) was amplified with the oligonucleotides psbA3/1 ... cushion in the absence of MgCl2 Under these experimental conditions, neither stroma nor LDMs entered the cushion [9,18], and could be collected together in one fraction, which contained the majority ... excess of dsDNA from the psbA 5¢ region had no effect on RBP63-binding (data not shown) In conclusion, these data confirm the high affinity of RBP63 for the psbA leader Analysis of the cis-acting...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: Hematopoietic differentiation from human ESCs as a model for developmental studies and future clinical translations Invited review following the FEBS Anniversary Prize received on 5 July 2009 at the 34th FEBS Congress in Prague docx
... result from multiple factors, including possible rejection by the host immune system, the quantity administered, the developmental stage of the cells derived from hESC differentiation, and the quality ... population of human cells derived from hESCs that were hypothesized to be the in vitro human equivalent of the hemangioblast, characterized by expression of the endothelial markers PECAM-1 (CD31), ... hematopoiesis from human ESCs from chronic myelogenous leukemia, suggesting that translocation of these genes occurred in a progenitor common to them both [44] Further supporting this hypothesis, both...
Ngày tải lên: 22/03/2014, 17:20
Security Risk Management: Building an Information Security Risk Management Program from the Ground Up doc
... apply them If they are spending all their time with enforcement, then either the educational aspects of the program are failing or they don’t have the necessary support from the leaders in the ... sharing with the rest of the industry We’ve come a long way from our early conversations at the Thirsty Bear All these mentors have either set me on the right track or given me a push in the right ... page that chose the points rather than the prize and the server accepted it each time When working with the developers to correct these flaws, their first inclination was to improve the client-side...
Ngày tải lên: 23/03/2014, 03:20
Training tips from the Digital Classroom Building stronger digital advertising sales teams pot
... As for the other media, that’s the methodology and the data that the Advertising Association publish through the guys at the WARC research centre If you have more questions then post them here ... simply not have the audience there that it needs The idea of capping stems partly from trying to extend the reach of the campaign (the number of people who see it) and partly from the need to avoid ... adspend – getting the numbers to add up Lots of people question whether the online adspend figures are accurate in the UK The details of the methodology are in the full report, but the bottom line...
Ngày tải lên: 30/03/2014, 12:21
adobe flex 4.5 fundamentals [electronic resource] training from the source
... different from the rest of the text Single lines of ActionScript code that are longer than the margins of the page are wrapped to the next line They are designated by an arrow at the beginning of the ... different from the rest of the text so you can identify them easily Training from the Source Code block: To help you easily identify ActionScript, XML, and HTML code within the book, the code ... structure Adobe Training from the Source The Adobe Training from the Source and Adobe Advanced Training from the Source series are developed in association with Adobe and reviewed by the product support...
Ngày tải lên: 29/05/2014, 13:56
báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... were isolated from the P putida strain NCIMB 10007 cultivated with camphor as sole carbon source The gene was then amplified by gradient PCR using gene specific primers derived from the corresponding ... Organics (Geel, Belgium) For DNA-purification from PCR, the MinElute PCR-purification Kit by Qiagen (Hilden, Germany) was used Furthermore the Miniprep Kit from Qiagen was used for plasmid purification ... (Uppsala, Sweden) The plasmid pET-28b(+) was from Novagen (Darmstadt, Germany) The BCA kit was purchased from Interchim (Montluçon, France) Amplification and cloning Amplification of the 2,5-DKCMO...
Ngày tải lên: 21/06/2014, 02:20
Spiritual MarketingA Proven 5-Step Formula for Easily Creating Wealth from the Inside Outby Joe pot
... greedy when they start to ask for what they want They feel they are taking from others The best way around that limiting belief is to be sure you also want others to have success, too In other words, ... turn then causes the anxiety The three can strike at lightening speed However, they can and must be stopped The primary cause of these debilitating demons is IGNORANCE Know the truth and the truth ... partners, but there they were side by side in perfect harmony What a beautiful couple they made They definitely belong together Yes, the marrying of these two words has created the potential...
Ngày tải lên: 28/06/2014, 22:20
Adobe Photoshop CS Tips and Tricks from the Experts - part 5 doc
... on the New Frame icon at the bottom of the Animation palette Select the last frame before you continue, then select the layer mask of the top layer in the Layers Palette Again, switch to the ... the layer mask Click on the layer mask of the second layer set (in this example the middle one) and change to the Move Tool in the Tool palette You can now move the layer mask while holding the ... but even easier, hold the shift key and hit the right arrow key until the layer mask is moved all the way to the right Move the second layer mask With the last frame (in the Animation window)...
Ngày tải lên: 05/08/2014, 23:21
Adobe Flex 4 Training from the Source Volume 1 phần 5 ppsx
... nodes from the XML returned from the server, regardless of whether they are under other nodes (such as the category node in this case) Download from www.eBookTM.com From the Library of ... object from the attributes of the XML node p 25 Still inside the for each in loop, use the push() method of the products array to add the newly created Product instance to the end of the products ... fields in the array is the order in which the sort fields are applied Set the sort property of the items ArrayCollection to the prodSort instance Then call the refresh() method of the items...
Ngày tải lên: 08/08/2014, 20:20
Báo cáo y học: "The shunt from the cyclooxygenase to lipoxygenase pathway in human osteoarthritic subchondral osteoblasts is linked with a variable expression of the 5-lipoxygenase-activating protein" doc
... shunt from the COX to the 5-LO pathway in human OA osteoblasts We also examined the implication of both 5-LO and FLAP in the production of LTB4 in these cells and the factors that might modulate their ... the exact mechanism underlying a shunt from the COX to the 5-LO pathway in osteoblasts remains unknown However, this knowledge could be crucial for therapeutic intervention in OA The actual therapies ... IL-18 In macrophages, the shunt from the COX to the 5-LO pathway is linked with a variable production of IL-10; as PGE2 levels rise, IL-10 levels increase and inhibit the synthesis of LTB4 [24]...
Ngày tải lên: 09/08/2014, 08:23
báo cáo khoa học:" Characteristics of maxillofacial injuries resulting from road traffic accidents – a 5 year review of the case records from Department of Maxillofacial Surgery in Katowice, Poland" pot
... 6,07%) There were more women than men in the group of car passengers In the other groups, men suffered from the injuries more often than women In all the groups, except for the cyclists, the difference ... the patients were grouped into age categories: from to 7, from to 17, from 18 to 25, from 26 to 40, from 41 to 55 and from 56 to 68 years In case of smaller number of observations disabling the ... between noon and PM, when the traffic intensity was the highest These results support the findings of the other authors that the most of traffic accidents occur at day time, when the atmospheric conditions...
Ngày tải lên: 11/08/2014, 23:22
Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine" doc
... the most dominant outbreak strain in the recent years and distributed around the world in over 24 countries [30] Towards the west, the virus spread into Saudi Aribia (1994), then emerged as the ... of the mutations in the whole genome of the O/Fujian/CHA/5/99 strain was based on multiple sequence alignments (Table 2) The S-fragment is 366 nucleotides in length at the 5’ terminus of the ... located at the 5’ terminus of the FMDV genome may play a role in the switch from translation to replication [34] The variable nature of PKs was documented that it can be used along with the 3A-based...
Ngày tải lên: 12/08/2014, 01:21
building vocabulary from word roots level 5
... complete these sentences using a word from the list Together choose one of the sentences to read out loud, but skip over the word from the list See if your classmates can guess the missing word Use the ... Conquer” Part A: Meet the Root Divide and Conquer Directions: “Divide” words and then “conquer” them by writing the meaning of the words Use the Root Bank at the bottom of the page to help you ... does not have a suffix Circle three words from the “has a suffix” column Write them below Then write them without the suffix With the suffix Without the suffix _ _ ...
Ngày tải lên: 13/01/2015, 14:38