4 h to set up on day of experiment
Báo cáo y học: "TNF inhibits production of stromal cell-derived factor 1 by bone stromal cells and increases osteoclast precursor mobilization from bone marrow to peripheral blood" ppt
... CXCR4 expression on target cells Although alternation of either of these could lead to impaired cell mobilization and homing, external factor regulation of SDF1 expression levels appears to be the ... and that TNF promotes the release of bone marrow OCPs into the bloodstream [3-5] To investigate whether the SDF-1/CXCR4 axis – the master chemokine system controlling mobilization of hematopoietic ... effect on OCP CXCR4 expression suggest that, like most hematopoietic cell mobilizers, TNF also promotes OCP mobilization through regulation of SDF-1 rather than through CXCR4 expression TNF-mediated...
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Báo cáo y học: "Pro-inflammatory properties of stromal cell-derived factor-1 (CXCL12) in collagen-induced arthritis" pptx
... destruction [5] Availability of specific inhibitors of the CXCL12-CXCR4 interaction has allowed the demonstration of the involvement of CXCL12 in experimental animal diseases One such inhibitor is the ... response to the chemokine CXCL12 Challenging mice with CXCL12 while they were treated with AMD3100 reduced the number of harvested cells to that of the negative control The number of cells in the air ... filled with PBS An air pouch assay was done on day 14 after minipump implantation (day 41 ) In both the AMD3100treated and the PBS-treated group, four of the mice received an injection into the air...
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Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx
... SDF-1 on HeLa cells depends on the HS chains of this PG To examine whether the tyrosine phosphorylation of SD -4 induced by SDF-1 on HeLa cells depends on HS, we treated these cells with mixtures of ... indicates that the HS-dependent binding of SDF-1 to SD -4 enables the chemokine to induce the homo- or hetero-oligomerization of its GPCR The physical association of tyrosine-phosphorylated SD -4 with tyrosine ... phosphorylation of SD -4 occurs in response to SDF-1 stimulation of HeLa cells This tyrosine phosphorylation depends on the time of incubation of the cells with the chemokine: marginal for 2-min incubation,...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... X-tremeGENE, Roche) The focus was then changed to retroviral vectors for introduction of the chTCR cassette into the human genome Retroviruses have the unique advantage of integration into all host cell ... absence of MHC [45 ] It is possible that LCK activation is a function of the surrounding membrane environment rather than the clustering of co-receptors with T cell receptor and MHC [46 ] Page of 10 ... Foundation (Houston, TX) The authors thank Hinrich Abken for the gift of a plasmid containing a cloned ScFv against CEA and Drs Abken and Zelig Eshhar for invaluable advice during the course of this...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... X-tremeGENE, Roche) The focus was then changed to retroviral vectors for introduction of the chTCR cassette into the human genome Retroviruses have the unique advantage of integration into all host cell ... absence of MHC [45 ] It is possible that LCK activation is a function of the surrounding membrane environment rather than the clustering of co-receptors with T cell receptor and MHC [46 ] Page of 10 ... Foundation (Houston, TX) The authors thank Hinrich Abken for the gift of a plasmid containing a cloned ScFv against CEA and Drs Abken and Zelig Eshhar for invaluable advice during the course of this...
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Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... expression controls The concentration of p 24 in culture supernatant is depicted in Figure 3A Both 5-HD and 8-HD cultures showed consecutively increasing levels of p 24 on days to On day of infection, ... Morphological changes in differentiated HD cells following exposure to HIV-1 Day- 0, HD cells which are on day th of differentiation and before viral infection Day- 3, shows the morphological changes of HD ... potential However, HIV exposure seems to expedite the generation of HD cells to less than days (from the normal days), when placed in hematopoietic differentiation media The generated HD cells from HIV-1...
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Cloning of medaka glial cell derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
... will then divide into chains of four Aaligned (Aal) spermatogonia which themselves, will further divide into chains of 8, 16 and up to, although rarely, 32 cells Upon reaching this stage, the spermatogonia ... illustrate that RET which moves to the lipid rafts upon stimulation by GDNF triggers the signal through FRS2 (fibroblast growth factor receptor substrate 2) while those that moves to the outside of the ... other hand, human GFRα1 has two isoforms; the longer isoform has 11 exons while the shorter one has one less (Fig 12) 30 Table Structure of gfrα1 with the predicted size of exons and introns and...
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báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
... another 12 months after the therapy had been terminated A scheme of the therapeutic approach and the course of the disease are summarized in Figure Identical to the protocol of the clinical phase I ... The expression of CD25 was considered here as a marker of lymphocyte activation since it did not show any correlation with the amount of CD4+ cells which would reflect the presence of regulatory ... 5th cycle) to 1.9 × 109 (highest value, 4th cycle), and this corresponded to 16% to 25% of the respective total lymphocyte population These parameters were not significantly different to those...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... difficult to inhibit for cancer therapy A better therapeutic approach, then, is likely to evolve from inhibition of the transcription factors promoting the suppressive phenotype Here we showed that HIF1a ... in healthy individuals [6] In our laboratory, induction of human MDSC from healthy donor PBMC by a one-week co-culture with select human cancer cell lines has allowed the generation of highly ... and Page 20 of 20 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 CD 14+ HLA-DR-/low myeloid-derived suppressor cells and decreased level of dendritic cells in patients with multiple myeloma...
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... between group and group On the other hand, an increase of cytochrome c in cytosol (Figure 7C), an index of mitochondrial damage, was notably higher in group than that in groups and However, no ... via a left thoracotomy Lung IR was then conducted in group and group animals on which a left thoracotomy was performed with the left main bronchus and blood supplies to the left lung totally clamped ... significantly higher in group than that in group Besides, mitochondrial cytochrome c (Figure 7B), an index of mitochondrial integrity, was notably reduced in group compared with that in groups and...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... lesions However, they were not sufficient to show changes of spinal cord lesions in the chronic phase In vivo MSC studies have demonstrated the cellular fate of cells which integrated into injured ... msec, with Hz and mA [41 ] The latency response was converted into velocity as a measure of spinal cord dysfunction The spinal conduction velocity from the 6th lumbar (L) vertebra to the 10th thoracic ... differentiate into neurons J Neurosci Res 2000, 61, 3 64- 370 40 Yang CC, Shih YH, Ko MH, Hsu SY, Cheng H, Fu YS Transplantation of human umbilical mesenchymal stem cells from Wharton's jelly after...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... pain due to osteoarthritis Due to her occupation, she made active use of her bilateral knee joints With a diagnosis of osteoarthritis of the right knee, she had received multiple injections of steroids ... pain due to osteoarthritis Her left knee was much more painful than the right Due to her occupation, she made active use of her bilateral knee joints With a diagnosis of osteoarthritis of Pak Journal ... of the right hip His pain had progressed over three years and he was offered a total hip replacement (THR) Being reluctant to proceed with the surgical procedure, he elected to proceed with stem...
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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
... response is critical for polarization of CD4+ T cells toward Th1 function, which is essential for the clearance of intracellular pathogens IL10, on the other hand, suppresses IL-12 production ... Page of 12 (page number not for citation purposes) Retrovirology 20 04, 1:37 expression of CD80 was up- regulated from 35% to 44 %, and the expression of CD86 from 49 % to 76% To see if the up- regulation ... infection (not shown), suggesting that LV infection may trigger a cascade of immune suppression through down-regulation of the NF-κB signaling pathway It has been reported that HIV-1 Tat up- regulates...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... differentiation 5.7 141 8 640 _at sir2 alpha 4. 3 145 6 341 _a_at kruppel-like factor 4. 1 142 4607_a_at xanthine dehydrogenase 3.7 145 2 240 _at bruno-like 4, rna binding protein (drosophila) 3.7 145 2179_at phd finger ... depending upon the stimuli This is further confirmed by the upregulated expression of leukemia inhibitory factor (LIF) in the BMP2+ population compared to the control EBs Interestingly, the transcripts ... NormalizedBMP2RT-PCRthegeneslivertheondone
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... levels of TLR4 on all three cell types is somewhat different from those of others [28,29] who found no expression of TLR4 on mDCs, but did show expression on moDCs However, there are studies showing ... after 24 h stimulation with LPS; levels of these markers were more than ten-fold higher in mature compared to immature cells The mDC models also showed up- regulation of these markers, but to a lesser ... intact Escherichia coli rather than LPS), culture conditions and cytokine detection method by the authors This pattern of cytokine production clearly makes mature mDCs more efficient in the cross-talk...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... technical help, tips and advices but also the camaraderie I am grateful for my family, especially my husband and son who have been with me through the tough times They have been nothing but supportive ... but the derivation of NSCs from adult brains is not possible Hence the derivation of NSCs from iPSCs is hoped to be able to overcome these hurdles, although there is a clear need to deremine the ... receptor 4( CXCR4) and vascular endothelial growth factor(VEGF)/VEGF receptor (VEGFR)-1 and VEGFR2 (Ehtesham M et al., 20 04; Schmidt NO et al., 2005) The fate of NSCs in the presence of lesions...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... adenosine action (Dunwiddie and Masino, 2001; Fredholm et al., 2005b) The anticonvulsant functions of adenosine are thought to base largely upon the activation of A1 receptors coupled to inhibitory G ... surrounding the exon, generating a downstream HA of 1165 bp length (Fig 3.1) The upstream HA was chosen to contain only intronic sequences found between exons and 10 with a length of 8 14 bp For the amplification ... of the protein Introduction of frame shifts within a coding exon of the gene prior to the catalytic part leads to destruction of all enzymatic activity Also shown is the reaction in which Arg316...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... the addition of the mitochondrial H+ -ATPase inhibitor oligomycin strongly reduced the oxygen consumption As the oligomycin-resistant portion of respiration reflects the level of proton leak of ... analysis has shown that only nonphysiological, high concentrations of UCP2 and UCP3 induced the changes in the mitochondrial proton permeability previously observed These uncoupling effects were the ... impairment of the mitochondrial function caused by the presence of a noncompetent hUCP3, rather than a decrease in mitochondrial number This hypothesis is supported by the lack of a specific effect of...
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