4 8 chuỗi xoắn kép của dna

... 30.7? ? 48 .2 0.69 0.67 0. 68 59.3 165 .4 8. 04 51.0 139.1 9.57 44 .8 rACCUAGUC/d 31.3? ?42 .7 1.26 1.26 1.26 58. 6 165.3 7.31 43 .4 115.5 8. 98 41 .0 rCUAGUGGA/d 34. 8? ? ?46 .7 0 .88 0 .88 0 .88 58. 2 161.6 8. 07 47 .7 ... 0.99 47 .3 123.2 9. 14 35.7 83 .4 10.79 52.5 dAGCGTAAG/d 31.6? ?44 .7 0. 38 0. 38 0. 38 47 .8 130.2 7.37 43 .2 115 .4 8. 81 42 .4 dATCTATCCG/d 37 .8? ? ?46 .7 1.03 1.11 1.07 54. 7 1 48 .5 8. 59 42 .3 1 04. 9 11.03 48 .0 dGCCAGTTAA/d ... 145 .8 6.76 48 .6 135.0 8. 34 38 .4 dAGTCCTGA/d 24. 4? ?40 .4 0.70 0.69 0.70 48 .8 133.2 7 .45 40 .4 105.9 8. 77 42 .7 dACGACCTC/d 35.9? ?47 .5 0 .42 0 .41 0 .42 55.2 150.5 8. 53 50.1 1 34. 5 10. 04 48. 2 dCTCACGGC/d 39.9–51.2

Ngày tải lên: 22/02/2014, 07:20

... 2163-21 74, doi:10.1007/s0 08 94- 011-12 34- x (2012) 91 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 Gell, D & Jackson, S P Mapping of protein-protein interactions within the DNA- dependent protein kinase ... 93 72 73 74 75 76 77 78 79 80 81 82 83 84 85 Dobbs, T A., Tainer, J A & Lees-Miller, S P A structural model for regulation of NHEJ by DNA- PKcs autophosphorylation DNA repair 9, 1307-13 14, doi:10.1016/j.dnarep.2010.09.019 ... heterodimer Biochemistry 47 , 43 59 -43 68, doi:10.1021/bi702 284 c (20 08) Yoo, S., Kimzey, A & Dynan, W S Photocross-linking of an oriented DNA repair complex Ku bound at a single DNA end The Journal of

Ngày tải lên: 24/08/2014, 09:33

... MDM2 34 1 .4. 3 The tumour suppressor p53 36 1 .4. 4 MDM2 –p53 autoregulatory feedback pathway 38 1 .4. 4.1 MDM2 blocks p53 transcriptional activity 38 1 .4. 4.2 MDM2 role ... 12, 3 48 8- 349 8 Zwijsen,R.M., Wientjens,E., Klompmaker,R., van der,S.J., Bernards,R., and Michalides,R.J (1997) CDK-independent activation of estrogen receptor by cyclin D1 Cell 88 , 40 5 -41 5 1 64 Oh ... (65) 8 74 3763 or (65) 8 74 3797 Fax: (65) 779 1117 or (65) 775 9 582 E-mail: mcblib@imcb.nus.edu.sg 7105 MOLECULAR AND CELLULAR BIOLOGY, Mar 19 98, p 1660–1669 0270-7306/ 98/ $ 04. 00ϩ0 Copyright © 19 98,

Ngày tải lên: 16/09/2015, 12:15

... effects on p53 ⁄ p73 DNA recognition 38 39 40 41 42 43 44 45 46 47 48 49 50 51 by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex FEBS J 273, 301–3 14 Vojtesek B, ... DNA response elements by the DNA binding domain of the tumor suppressor protein p53 J Biol Chem 272, 1 48 42– 1 48 49 47 04 11 Kim E & Deppert W (2003) The complex interactions of p53 with target DNA: ... Czech Republic Fax: +42 0 541 211293 Tel: +42 0 541 517197 E-mail: fojta@ibp.cz (Received 25 April 2006, revised 18 July 2006, accepted 17 August 2006) doi:10.1111/j.1 742 -46 58. 2006.0 547 2.x Proteins p53

Ngày tải lên: 19/02/2014, 05:20

... 28, 43 4? ?44 5. 28 Noirot-Gros MF, Dervyn E, Wu LJ, Mervelet P, Er- rington J, Ehrlich SD & Noirot P (2002) An expanded view of bacterial DNA replication. Proc Natl Acad Sci USA 99, 8 342 ? ?8 347 . 29 ... 2e0g, 2wp0) 74 )8 .4 PolC-NII, other (5) Models (based on 2aya, 2e0g, 2wp0) 74 ()7 .8; )8. 2) Reference structures PolIIIs-V, E. coli NMR, 2aya 72 )8. 0 DnaA-I, E. coli NMR, 2e0g 77 )5.6 DnaA-I, H. ... mechanisms in inter-DnaA interaction and in DnaB helicase loading on oriC. J Biol Chem 282 , 1 781 6–1 782 7. 21 Grishin NV (2001) KH domain: one motif, two folds. Nucleic Acids Res 29, 6 38? ?? 643 . 22 Margelevic ˇ ius

Ngày tải lên: 05/03/2014, 23:20

... 40 0005, India Fax: +91 22 2 280 46 10; 2 280 46 11 Tel: +91 22 22 78 2606 E-mail: bjrao@tifr.res.in (Received 20 June 2005, revised 19 August 2005, accepted 28 September 2005) doi:10.1111/j.1 742 -46 58. 2005. 049 97.x ... 1 48 22– 1 48 27 17 Allen DJ, Makhov A, Grilley M, Taylor J, Thresher R, Modrich P & Griffith JD (1997) MutS mediates heteroduplex loop formation by a translocation mechanism EMBO J 16, 44 67? ?44 76 18 ... 2 74, 5 9 48 –5952 28 Grilley M, Welsh KM, Su S-S & Modrich P (1 989 ) Isolation an Characterisation of Escherichia coli mutL J Biol Chem 2 64, 1000–10 04 29 Worth L Jr, Bader T, Yang J & Clark S (19 98)

Ngày tải lên: 07/03/2014, 12:20

... pulse schemes [41 ,44 ] in an interleaved manner to collect six points with delays of 14, 210, 42 0, 700, 1191, 1 542 ms for T1, and six points with delays of 8. 2, 24. 5, 40 .8, 57.1, 73 .4, 97.9 ms for ... to DNA- binding function J Virol 71, 82 8? ?83 1 Ferreiro D, Dellarole M, Nadra AD & De Prat-Gay G (2005) Free energy contributions to direct readout of a DNA sequence J Biol Chem 280 , 3 2 48 0–3 2 48 4 ... side (A) ˚ Simulation box Z side (A) Saved configurations 31 750 31 34 200 95 34 14 77 56 83 10 160 23 46 8 26 64 162 6930 14 56 73 64 10 160 where the averages were computed over the equilibrated MD

Ngày tải lên: 16/03/2014, 10:20

... Sciences of the Czech Republic, Kralovopolska 135, CZ-61265 Brno, Czech Republic Fax: +42 0 541 240 499 Tel: +42 0 541 517 1 48 E-mail: brabec@ibp.cz URL: http://www.ibp.cz/labs ⁄ BNAIAD *The authors wish it ... [{trans-PtCl(NH 3 ) 2 ] 2 l-trans- Pt(NH 3 ) 2 {H 2 N(CH 2 ) 6 NH 2 ] 2 ] 4+ (BBR 346 4) reduce the binding affinity of the modified DNA to p53 protein even markedly more efficiently than the adducts of cisplatin [ 14] . Interestingly, BBR 346 4 retains significant activity ... subse- quently against water at 4 °C, and the platinum con- tent was determined by FAAS (Fig. 4) . The reaction of DNA with trans-PtHMP was com- plete after 48 h (Fig. 4) . Thiourea displaced c. 97% trans-PtHMP

Ngày tải lên: 16/03/2014, 14:20

... U DNA ligase (Weiss units in the case of T4 DNA ligase) at 16 °C (T4 DNA ligase and E.coli DNA ligase) or 45 °C(Taq DNA ligase and H. Kuhn and M. D. Frank-Kamenetskii Ligation of ssDNA by T4 DNA ... 02215, USA Fax: +1 617 35 385 01 Tel: +1 617 353 84 9 2 E-mail: hkuhn@bu.edu (Received 11 July 2005, revised 30 August 2005, accepted 2 September 2005) doi:10.1111/j.1 742 -46 58. 2005. 049 54. x T4 DNA ligase is one ... were T4 DNA ligase, E. coli DNA ligase, Taq DNA ligase, and Ampligase (lanes 4? ??7), respectively. Lanes 1–3 are controls in the absence of both I and T4 DNA ligase, and in the absence of either T4 DNA ligase

Ngày tải lên: 23/03/2014, 11:20

... 3 180 75.0 Pol d (5 nm) 6967 2097 30.1 139 3 04 Pol e (10 nm) 3 181 2 342 73.6 T98G Pol d (10 nm) 1939 581 30.0 54 131 Pol e (5 nm) 19 34 12 58 65.0 Human DNA polymerases a, d and e in replication A. ... replication causes DNA damage J Cell Sci 17, 48 97? ?49 08 44 Nakamura H, Morita T, Masaki S & Yoshida S (19 84 ) Intracellular localization and metabolism of DNA polymerase a in human cells visualized ... 231–232 3 Lehman IR & Kaguni LS (1 989 ) DNA polymerase a J Biol Chem 2 64, 42 65? ?42 68 4 Bullock PA, Seo YS & Hurwitz J (1991) Initiation of simian virus 40 DNA synthesis in vitro Mol Cell Biol

Ngày tải lên: 30/03/2014, 10:20

... Journal 2005, 2:55 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 regulation of both DNA synthesis and viral replication J Virol 1996, 70(5):30 18- 3025 Reddy SM, Williams ... to ppUL 44. Arrows designate the specific bands. Recruitment of pUL1 14 to the nucleus by ppUL44Figure 7 Recruitment of pUL1 14 to the nucleus by ppUL 44. Plasmids expressing ppUL 44 or pUL1 14- EGFP ... coexpression of ppUL 44 (Texas red), pUL1 14- EGFP (green) and a merged image show that ppUL 44 can recruit pUL1 14 to the nucleus. In third row of images ppUL 44 (Texas red) and pUL1 14- EGFP containing

Ngày tải lên: 19/06/2014, 08:20

... A [GenBank:NP_ 043 87 8 ], Mastadenovirus-human adenovirus C [GenBank:NP_ 040 516], Ata- denovirus-duck adenovirus 1 [GenBank:NP_ 044 702 ], Siadenovirus-frog adenovirus [GenBank:NP_06 243 5], α Herpesvirus- ... [GenBank:NP_ 044 632 ], β Herpesvirus1-human herpesvirus 5 [GenBank:P 08 546 ], β Herpesvirus2-human herpesvirus 6 [GenBank:NP 042 931 ], γ Herpesvirus-human herpesvirus 4 [GenBank:NP_0399 08] , Ictalurid ... virus. J Virol 2000, 74: 381 5- 383 1. 3. Braithwaite DK, Ito J: Compilation, alignment, and phyloge- netic relationships of DNA polymerases. Nucleic Acids Res 1993, 21: 787 -80 2. 4. Villarreal LP, DeFilippis

Ngày tải lên: 20/06/2014, 01:20

... Journal 2005, 2:55 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 regulation of both DNA synthesis and viral replication J Virol 1996, 70(5):30 18- 3025 Reddy SM, Williams ... to ppUL 44. Arrows designate the specific bands. Recruitment of pUL1 14 to the nucleus by ppUL44Figure 7 Recruitment of pUL1 14 to the nucleus by ppUL 44. Plasmids expressing ppUL 44 or pUL1 14- EGFP ... coexpression of ppUL 44 (Texas red), pUL1 14- EGFP (green) and a merged image show that ppUL 44 can recruit pUL1 14 to the nucleus. In third row of images ppUL 44 (Texas red) and pUL1 14- EGFP containing

Ngày tải lên: 20/06/2014, 04:20

... behavior of DNA stretching under different conditions [43 ]. The different forces and their influence on DNA stretching have yet to be fully understood. Germishuizen et al. [44 ] conducted DNA stretching ... Nanoscale Res Lett (2007) 2: 185 –201 189 123 model for DNA that can be applied to DNA under high tensions [31]. Bensimon and coworkers have pioneered the study of DNA? ??protein interactions using ... real-time replication rate of a single DNA polymerase (DNAP) on a single-stranded DNA (ssDNA) stretched by a magnetic trap as shown in Fig. 5A. Similarly to RNAP, DNAP reads the base sequence information

Ngày tải lên: 22/06/2014, 18:20

... thế chỗ của DNA polymerase δ tiếp tục vừa kích thích hoạt động của protein Dna2 . Chuỗi Nu bị thế chỗ sau đó có thể bị cắt bởi hoạt tính endonuclease của Dna2 , chừa lại một đuôi chuỗi đơn ... hoạt động kế tiếp nhau của ba protein: RPA, Dna2 và FEN-1. DNA polymerase δ trước tiên sẽ thế chỗ đoạn RNA primer và đoạn DNA do DNA polymerase α tổng hợp. Chuỗi DNA đơn sau đó sẽ được bao ... trình tiến hóa của DNA và hệ thống nhân đôi DNA (p-2) Sự tiến hóa của các cơ chế đặc thù liên quan đến sự nhân đôi của DNA: Hai nghiên cứu điển hình. Sự hiểu biết thêm của chúng ta

Ngày tải lên: 02/07/2014, 11:21

... and BO1051 treatment Irradiation dose (Gy) Relative reduction (%) U87MG U251MG GBM-3 41 .7 42 .6 40 .6 70 .4 45.6 47 .0 74. 2 47 .9 64. 3 76.3 50.0 73.9 Percentage relative reduction of the observed surviving ... 19 98, 273(10): 585 8- 586 8 43 Sedelnikova OA, Rogakou EP, Panyutin IG, Bonner WM: Quantitative detection of (125)IdU-induced DNA double-strand breaks with gammaH2AX antibody Radiat Res 2002, 1 58 (4) : 48 6 -49 2 ... Denny WA: DNA- directed alkylating agents Synthesis and antitumor activity of DNA minor groovetargeted aniline mustard analogues of pibenzimol (Hoechst 332 58) J Med Chem 19 94, 37(25) :43 38 -43 45 Gourdie

Ngày tải lên: 09/08/2014, 09:20

... phân tử DNA mới (bán bảo toàn). • (3) Mồi RNA (4- 12 nucleotide) giúp DNA pol kéo dài chuỗi polynucleotide. • (4) Các enzyme và protein, bao gồm: ∀ • DNA pol kéo dài DNA đang tăng trưởng ∀ • DNA ... TÁI BẢN DNA VÀ SỬA CHỮA DNA 1. Các đặc tính và yếu tố thiết yếu của sự tái bản 2. Cơ chế của sự tái bản 3. Sự tái bản ở tế bào chân hạch 4. Sửa chữa DNA • Nguyên phân (tạo ... tế bào con) & giảm phân (tạo 4 tế bào con) đều cần sự nhân đôi nhiễm sắc thể → cần nhân đôi DNA (tái bản) • 2. Các đặc tính và yếu tố thiết yếu của sự tái bản • Các đặc tính ∀ • Theo

Ngày tải lên: 10/08/2014, 23:22

... 25.3° 46 .8? ? 35.9° 29.5° #2 30.1° 28. 1° 17 .4? ? 39.2° 32.0° 23.7° #3 42 .3° 36.5° 28. 7° 54. 5° 36.5° 34. 6° Average 36.0° 32.3° 23 .8? ? 46 .8? ? 34. 8? ? 29.3° *Angle is in degrees and reflects pLuc DNA degradation ... Drug Dev Ind Pharm 20 08, 34: 943 - 947 . doi:10.1 186 / 147 7-3155 -8- 6 Cite this article as: Zhong et al.: Optimization of DNA delivery by three classes of hybrid nanoparticle /DNA complexes. Journal ... Table 4 Zeta potential of nanoparticle DNA complexes Formulation Zeta Potential (mv) A1 DOTAP (out) 06 .86 ± 0.72 B1 DC-Chol (out) 05 .83 ± 0. 24 C1 DOTAP (in) 31.95 ± 0.99 D1 DC-Chol (in) 14. 84 ±

Ngày tải lên: 11/08/2014, 00:22

... (same day) 100,000 17.23 97 ,8 84 9, 144 8. 08% 9. 34 10,000 20.91 9 ,85 2 542 4. 45% 5.50 1,000 100 24. 38 28 .43 1, 146 94 202 19 16.12% 17.70% 17. 64 20.51 10 31.95 11 35. 38% 0.9 94 41.91 BHRF-1 Intra-Assay ... Variation 100,000 19 .87 101, 644 14, 0 58 10.99% 28. 35 10,000 23.75 10,660 1 ,47 1 13.65% 13 .80 1,000 27. 68 1,0 84 191 18. 67% 17.60 100 31.71 111 49 33. 58% 43 .75 10 35 .86 12 65.03% 0.990 75 .85 BHRF-1 Inter-Assay ... Variation (different days) 100,000 18. 05 105, 387 4, 621 6.02% 4. 38 10,000 21.75 9,779 81 8 6.23% 8. 37 1,000 25.23 1, 042 141 11.63% 13.55 100 29.06 89 18 13.76% 19 .88 10 32.66 12 25.30 0.997 16.53 EBNA-1

Ngày tải lên: 12/08/2014, 01:22

... stability in plants Nature 2000, 40 6(6791): 98- 101 Zhou BB, Elledge SJ: The DNA damage response: putting checkpoints in perspective Nature 2000, 40 8( 681 1) :43 3 -43 9 Wu YQ, Hohn B, Ziemienowic A: ... Mutagen 2005, 45 (2):115-127 Johnston LH, Nasmyth KA: Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase Nature 19 78, 2 74( 56 74) :89 1 -89 3 Petrini JH, Xiao Y, Weaver DT: DNA ligase ... 5(1):1 Friesner J, Britt AB: Ku80- and DNA ligase IV-deficient plants are sensitive to ionizing radiation and defective in T -DNA integration Plant J 2003, 34( 4) :42 7 -44 0 Li J, Vaidya M, White C,

Ngày tải lên: 12/08/2014, 03:20