4 unrelated cord blood banking

... 30 minutes and then washed twice with PBS 1% BSA followed by detection of incorporated EdU in accordance with the manufacturer’s instructions Flow cytometric analysis was performed on a FACSCalibur ... (University of Leuven) Measurement of anti-CII antibodies and delayed-type hypersensitivity to CII Blood samples were taken from the orbital sinus or by heart puncture and were allowed to clot at ... Rheumatol 2009, 5:392-399 Nauta AJ, Fibbe WE: Immunomodulatory properties of mesenchymal stromal cells Blood 2007, 110:3499-3506 Siegel G, Schafer R, Dazzi F: The immunosuppressive properties of mesenchymal...

Ngày tải lên: 12/08/2014, 11:23

... mineralization indicated by arrow in fig (d ), (c) H&E staining showing newly formed tissue (arrow showing blood vessel infiltration into newly formed tissue) 45 Figure week time-point (b) ... Figure 11 At week time-point: (a) H&E staining (c) H & E showing newly formed tissue (arrow showing blood vessel), (b) von Kossa staining did not show the presence of mineralised tissue ... and around the bone are softer structures such as the vital organs, muscles, cartilages, nerves, blood vessels, adipose tissue and skin Bone also serves as a reservoir for minerals such as calcium,...

Ngày tải lên: 09/10/2015, 11:24

... declined upon further differentiation [25]; it was also downregulated in erythropoietic culture of cord blood CD34+ progenitor cells [47] Selected differentially expressed genes identified by microarray ... been reported to be highly expressed in hematopoietic stem cells (HSCs) from human umbilical cord blood, and it promotes differentiation of HSCs into progenitor cells by inhibiting ABCG2 expression[56] ... Inc, Walkersville, MD), and Lung Fibroblasts (LFB) (ATCC) All of the adult cells were cultured according to manufacturer's protocol MiRNAs expression profiling A miRNA probe set was designed using...

Ngày tải lên: 18/06/2014, 15:20

... microarray Total RNA from two groups of SP cells was isolated using TRIZOL reagent (Invitrogen) according to the instructions of the supplier and was further purified using an RNeasy mini kit (Qiagen, ... Sanger’s miRBASE version 13.0 were spotted onto the slides The hybridization was carried out according to the manufacturer’s instructions; a 635 nm laser was used to scan the slide using the ... cells and are responsible for cellular heterogeneity of cancer due to aberrant differentiation According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived...

Ngày tải lên: 10/08/2014, 10:20

... applied via inhalation with a conventional jet nebulizer (PARI-BOY, Pari, Starnberg, Germany) according to Baumgartner et al [8] The patients were randomized to receive 5-ALA 1, 2, 3, or hours ... intensity of autofluorescence, PPIX fluorescence, and diffuse backscatter at 520, 635 and 840 nm according to the following equation: [PPIX] ~ [I(635 nm)-0.65*I(520 nm)]/I(840 nm) (1) PPIX = porphyrin ... findings were classified as "(Pre)Malignant" The application-time dependent spectral PPIX values according to equation were fitted for the benign (≤ mild dysplasia) and the (pre)malignant (≥ moderate...

Ngày tải lên: 12/08/2014, 15:20

... avoid viral spread and contamination Animal care and experimental procedures were performed in accordance with the requirements of the National Institute of Agricultural Technology Ethics Committee ... ml of E-MEM Clinical observation of respiratory and nervous signs, and rectal temperature were recorded in an individual sheet for each animal Between days 35-37 post infection (pi) animals were ... necropsy was performed immediately after euthanasia Transverse sections from the cervical spinal cord, olfactory cortex, frontal, parietal and occipital cortex, thalamus, mesencephalon, cerebellum,...

Ngày tải lên: 12/08/2014, 18:22

... and Use Committee (IACUC) guidelines Synthesis and characterization of PA PA was synthesized according to the method described in previous literature (Jung et  al., 2003; Zhang et  al., 2009) ... than 3.5 ppm Preparation and characterization of PANs and PA/EPI Nanoparticles were prepared according to a solvent diffusion method (Fessi et al., 1989; Govender et al., 1999; Bilati et al., ... on 03/01/11 For personal use only In vivo toxicity The toxicity of PANs was evaluated in vivo according to the previous reported method (Yoksan & Chirachanchai, 2008; Sonaje et al., 2009) Adult...

Ngày tải lên: 23/04/2013, 21:38

... UP12 was purified from the cell extract by affinity chromatography using His-bind resin (Novagen), according to the manufacturer’s instructions The protein was eluted with buffer K containing M imidazole ... UP12 were separated by SDS/PAGE along with the cell extract samples Immunoblots were performed according to the ECL Western blotting protocol (Amersham), and the chemiluminescence was detected ... of action of UspA, as well as that of other members of the UspA family remains to be explored According to our findings, it is clear that UP12 expression is induced under various stress conditions...

Ngày tải lên: 22/02/2014, 07:20

... nm (D600 nm) with a mL HiTrap chelating column (Pharmacia Biotech), loaded with nickel ions, according to the manufacturer’s instructions The cytoplasmic fraction, supplemented with 300 mm NaCl ... denatured proteins, the fluorescence emission spectra between 530 and 750 nm of the solutions were recorded on a Shimadzu RF 5301 PC spectrofluorophotometer, using excitation wavelengths of 406 nm for ... described [6] Catalase activity was assayed as described previously [6] Light absorption spectra were recorded with a Shimadzu UV-2101PC spectrophotometer Metal content analysis was performed by using...

Ngày tải lên: 06/03/2014, 11:20

Ngày tải lên: 07/03/2014, 16:20

... identified in mice killed after 24 and 48 h (data not shown) There was no radioactivity found in blood or heart tissue at any of the time points studied and no major differences were found between ... peak appearance of antigen-derived label 24 h after administration of labelled antigen [23] In accordance with these findings we found that lung-associated lymph nodes were radioactively labelled ... and water ad libitum The study was approved by the Regional Animal Research Ethics Committee according to national laws Preparation of allergen House dust mite extract was prepared from D pteronyssi¨...

Ngày tải lên: 07/03/2014, 21:20

... Healthy leaves from typically 5–10 Arabidopsis plants were pooled Thylakoid membranes were isolated according to [26] PSI particles were isolated from thylakoids after solubilization with dodecyl-b-d-maltoside...

Ngày tải lên: 07/03/2014, 21:20

... h and 12.00 h Rats were anesthetized with Pentothal (50 mgÆkg)1 body weight, intraperitoneally) Blood was collected from the aorta in heparinized syringes and then liver and lung samples were...

Ngày tải lên: 08/03/2014, 08:20

Ngày tải lên: 08/03/2014, 22:20

... Dynamic tracking of human hematopoietic stem cell engraftment using in vivo bioluminescence imaging Blood 102, 3478–3482 10 Kim KI, Chung JK, Kang JH, Lee YJ, Shin JH, Oh HJ, Jeong JM, Lee DS & Lee...

Ngày tải lên: 16/03/2014, 01:20

... (22 ± °C; humidity, 50 ± 5%; lighting, 8–20 h) Systolic blood pressure was measured by tail-cuff plethysmography [W&W Electronic, BP recorder 8005 (Huntsinlle, AL, USA)] on prewarmed (37 °C) ... originally described by Byrom and Wilson [38] Normotensive and hypertensive rats showed mean arterial blood pressures of 100 ± and 145 ± 10 mmHg, respectively Experimental hypertension Experimental ... Science Publisher 38 Byrom FB & Wilson CA (1938) A plethysmographic method for measuring systolic blood pressure in the intact rat J Physiol 93, 301–304 39 Bradford MM (1976) A rapid and sensitive...

Ngày tải lên: 30/03/2014, 04:20

... performed without any knowledge of which treatment each animal had received Rats were cared for in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Institutes ... Mol/ L) of VEGF15 (n = 6), VEGF165 (n = 7), or QK (n = 8) Digital Angiographies and Collateral Blood Flow Determination Rats were anaesthetized as described above and the left common carotid ... peptide-infused HL (VEGF15:38 ± 3; p < 0.05, ANOVA) as depicted in Figure 2A Regional gastrocnemius blood flow was also measured by dyed microspheres entrapment after intra-aortic infusion After muscle...

Ngày tải lên: 18/06/2014, 15:20

... analysis of the expression of the two target genes in a panel of human breast cancers classified according to Sotiriou and Pusztai [22] as the subtypes luminal A, luminal B, HER-2 and triple negative, ... 2006) All tumor samples used in this study were characterized with respect to their TNMG status according to WHO specifications following the criteria of the International Union Against Cancer UICC ... The second cohort of specimens consisted of 58 primary infiltrating breast tumors classified according to Sotiriou and Pusztaim [22] into the four distinct subtypes: luminal A, luminal B, HER-2...

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 20/06/2014, 01:20