4 postimplantation remodeling and in vivo recruitment of microvascular networks

... Table of contents Table of contents ii Summary v List of abbreviations vii List of tables ix List of figures ix Chapter Overview of research ... mM PDCA and 10 μM HDZ showed blood vessel infiltration comparable to VEGF delivery, and at this point, served as a proofof-concept that local delivery of PHi can aid angiogenesis vi List of abbreviations ... prophylactic setting of small skin wounds [61] 2.3 Understanding the last mile of the fibrotic pathway Irrespective of upstream events that trigger and entertain fibrosis, the final product of cellular...

Ngày tải lên: 10/09/2015, 15:52

... thanks to the staff of Highlander’s Coffee cafe for the wonderful coffee and great environment for us to brainstorm our ideas and work Everyone in NUS Nanobiomechanics Laboratory and NUS Regenerative ... period of days Immunofluorescent staining of hMSCs showed an upregulation of synaptophysin, indicating the establishment of synapse and electrical communication between cells Upregulation of connexin ... Quantum-dot labeling of collagen in PEC fibers and detection of collagen in (A) Sub-micron fibrils, (B) and (C) main fiber and (D) fiber bead region 70  Figure 4.11: Swelling studies of PEC and Polypyrrole...

Ngày tải lên: 11/09/2015, 09:10

... casting and solvent-free method of hot roll-milling • The exploration of the use of lasers to modify the surface of PCL films and understanding the mechanisms behind it, and the effect of the ... and elongation (λ) of all types of PCL films (Page 52) Table 4.1 Thermal and optical properties of PCL (Page 67) xi List of Figures Fig 2.1 Comparison of moduli of elasticity of biomaterials [15] ... the degree of Doctor of Philosophy in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Associate Professor Hong...

Ngày tải lên: 14/09/2015, 08:36

... usually show only γ-γ dimers and loss of α and γ bands as well as a-a multimers (Fig 2) Determine the density of the FMB by layering an aliquot of it onto a sucrose solution of known density After ... between HA and the coupling reagent is chosen as a function of the degree of substitution of the ester For a total esterification (>90% of carboxyl groups esterified), use an excess of 25–30% of benzyl ... biocompatible, absorbable, and easily and reproducibly processable, and the surface of the material should interact with cells and tissues (31) The material should not transfer antigens, and it should be...

Ngày tải lên: 10/04/2014, 11:11

... with a step size of 0.02° and a step time of s The powder samples were placed on a flat silicon zero background sample holder The particle size distribution of a regular suspension and nanosuspension ... chromatographic tandem mass spectrometric (LC/MS/MS) The stability of the DCU formulations (both regular suspension and nanosuspension) was assessed, and no issue was found No particle size, potency, and ... dosing of 30 mg/kg of DCU nanosuspension twice a day (b.i.d.) is needed to provide continuous coverage of the plasma concentrations of 0.2 μM (1 × cellular IC50 corrected for free fraction) and...

Ngày tải lên: 21/06/2014, 03:20

... predisposes the patient to tooth mobility and loss, affects at least 15% of adults over the age of 30 years of age [51] In the US, the economic cost of treating and preventing periodontal disease was ... delivery of the DNA of interest to the pulpal/periodontal tissue, or the isolation of a small amount of gingival tissue from the patient, transduction/transfection of the DNA at chairside, and reimplantion ... similarity to the human periodontium in terms of structure, plaque flora, and inflammatory infiltrate), and beagle dogs (which have a different microflora and much faster bone turnover rate compared...

Ngày tải lên: 11/08/2014, 23:22

... cultivated and fabricated tissues may be used for studying the primary mixing of cells, and the patterns of cell differentiation and growth within growing spheres in order to improve the outcome of microsphere ... development of high-throughput systems, and allow manipulation of individual spheres It seems worthwhile elaborating new bioreactor technologies and culture techniques to improve the ex vivo growth of ... the composition of the extracellular matrix This may be accomplished in a variety of fashions, including differential expression of particular extracellular matrix components and/ or proteases...

Ngày tải lên: 11/08/2014, 23:22

... receptor x List of Tables Table 1.1 Summary of the advantages and limitations of the most commonly used vascularization strategies Table 2.1 A selected list of prolyl hydroxylase inhibitors and their ... tissue availability and donor site morbidity, while allogeneic and xenogeneic transplants have problems of immunogenicity and risks of disease transmission [4, 5] In addition, the demand for allogeneic ... stabilized and is thus not secreted, resulting in decreased collagen deposition Based on the understanding of collagen P4H’s structure, stereochemistry, and mechanism of action, a variety of small...

Ngày tải lên: 09/09/2015, 11:11

... of typical steps like: • • • • • Development of a control process model Design and programming of a first simple control Calibration of sensors and actuators Conception of performance tests and ... for demonstrations of a forklift handling device and optical line following system A rock and sand landscape similar to Mars, including facilities for a tether link to power and control rovers ... WWW-server that interfaces with clients and handles over the Internet the transfer of sensor data from the operator workplace and commands to the rover The WWW-server and the operator workplace exchange...

Ngày tải lên: 05/03/2014, 15:20

... 2) and every months after the end of VMAT Statistical analysis OS and PFS were calculated using the Kaplan Meier method [17] Recorded events were death (all causes of Page of death included) and ... Hospital/University of Geneva, CH-1211 Geneva 14, Switzerland 2University of Geneva, CH-1211 Geneva, Switzerland Authors’ contributions DCW was responsible for the primary concept and the design of the study; ... 31.2 and 23.8 ± 30.5 before and months after VMAT, respectively (p = 0.48; Table 3) MMSE, KPS and self-assessed QoL were also compared for the time points before and months after the start of VMAT...

Ngày tải lên: 09/08/2014, 09:20

... implementation of immunosuppression to minimize the risk of graft rejection Special attention should also be paid to the delivery of implant cells to the recipient site and providing boosting Page of and recruitment ... injuries and the regeneration of the epithelial structure involve stem and progenitor cells The mechanism of the regeneration of the airway epithelium was widely studied in rodent models of lung ... mechanical and enzymatic digestion of the adult human lung tissue revealed the presence of mixed phenotype cells with type II alveolar and Clara cell features and high expression of SC regulatory...

Ngày tải lên: 10/08/2014, 05:21

... PM, Bieniasz P, Hahn BH, Hatziioannou T, Kirchhoff F: Tetherin-driven adaptation of Vpu and Nef function and the evolution of pandemic and nonpandemic HIV-1 strains Cell Host Microbe 2009, 6:409-421 ... Page of 13 Figure Mutation of S52A impairs Vpu-mediated degradation of CD4 and tetherin (A) Western blot analysis of Vpu expression in lysates of transfected 293T cells (B) FACS analysis of CD4 ... http://www.retrovirology.com/content/7/1/1 Page of 13 Figure Modulation of tetherin and CD4 in primary T-cells and macrophages by Vpu (A) FACS analysis of CD4 and tetherin modulation in infected PBL...

Ngày tải lên: 12/08/2014, 23:22

Ngày tải lên: 21/08/2014, 10:51

... my two supervisors Prof Teoh Swee-Hin and A/Prof Jerry Chan I would like to thank them for their constant sharing of knowledge and ideas, their patience and understanding, and the many hours they ... providing the bulk of the tensile yield strength and plays an important in the storage of various ions The mineral salts of bone comprises of largely of calcium and phosphorus (Downey and Siegel 2006) ... first days of culture Figure 7-3 (A, C) Experimental work-flow of cellular-treatment before and during Matrigel assay and (B, D) quantification of quantification of branch points of vessel networks...

Ngày tải lên: 09/09/2015, 10:16

... Formation of CLS not follow scaffold fiber orientation LIST OF TABLES Table Summary of the in vitro and in vivo angiogenic and anti-fibrotic effects of PDCA, CPX, HDZ and VEGF xi LIST OF ABBREVIATIONS ... Effects of various concentrations of HDZ, CPX, PDCA and 10ng/ml VEGF on optimized sequential co-cultures of fibroblasts and HUVEC Figure 14 Suramin inhibits angiogenesis in sequential co-cultures and ... cytotoxicity of HDZ, CPX and PDCA in monocultures or mixed cultures of IMR90 fibroblasts and HUVECs Figure 32 PHi inhibition of fibroblast collagen production in vitro SDS-PAGE and desitometric...

Ngày tải lên: 12/09/2015, 08:18

... layer of the periosteum is a 3D layer comprising of highly fibrous tissues and vascular network, hence mimicry of this layer would require 3D integration of cells and scaffold Modifications of the ... pre-determined period of time resulted in tough yet flexible nanofiber sheets (Fig 4B, C) that could be handled for downstream applications Morphology and size distribution of PCL and PCL/Col nanofibers were ... group and was even more pronounced in PCL/Col-Hep After ten days of culture, cells were able to penetrate approximately half of the thickness of PCL/Col and throughout the structure of PCL/Col-Hep...

Ngày tải lên: 14/09/2015, 08:24

... degree of Doctor of Philosophy in the Graduate Program in Bioengineering at the National University of Singapore under the supervision of Professor Lee Chuen Neng, Professor Teoh Swee Hin and Dr ... years and has saved many lives, while having improved the quality of lives of thousands of other patients Paradoxically, the advent of reliable transplantation techniques created the issue of organ ... formation of a confluent EC layer in vivo and evidence of tissue ingrowth and vitalisation of the construct (Greisler 1985; Greisler 1987) However, the extent of tissue formation was insufficient, and...

Ngày tải lên: 14/09/2015, 08:49

... maintenance and substitution of damaged tissues and organs using principles of biology and engineering (Langer and Vacanti, 1993) BTE consists of an interplay of scaffold technology, growth factors and ... techniques, the understanding of the structure and cells which participate in bone repair is of utmost importance Bone is composed of around 70-90% of minerals with the rest in the form of proteins Within ... step of degradation When the molecular weight of polymer decreases to about 5000, second step of degradation will commence The rate of chain scission and weight of polymer decreases as a result of...

Ngày tải lên: 09/10/2015, 11:18

... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... harvesting grafts x LIST OF TABLES Table 3.1 Mw, Mn, and PDI of NaOH-treated and lipase-treated PCL-TCP Scaffolds 40 Table 4.1 Mw, Mn, and PDI of native, NaOH-treated, and lipase-treated PCL-TCP ... months of implantation of autografts and PCL-TCP scaffolds for individual micropigs 99 Figure 5.14 The average values of bone volume fraction detected after months of implantation of autografts and...

Ngày tải lên: 09/10/2015, 11:18