4 molecular biology of germ cell tumors and effect of targeted therapy

... higher cellular density and a notable presence of atypia and mitotic cells Grade IV tumors are the most malignant and also the most frequent gliomas and include glioblastoma and gliosarcoma These tumors ... Evolution of the Molecular Biology of Brain Tumors and the Therapeutic Implications nectin is almost absent but it is effective in the microenvironment of glioma cells, which coexpress large amounts of ... of communication to other cell cell junctions and to cellsubstratum junctions Cadherin cluster forms a transmembrane core of adherens junctions at sites of the cell cell contacts During tumor...

Ngày tải lên: 23/03/2014, 10:20

... al BMC Plant Biology 2011, 11:60 http://www.biomedcentral.com/1471-2229/11/60 Page of 14 Figure Characteristic features of organelle markers and subcellular location of proteins of flower-abundant ... filters were 458 nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710 ... represented in tandem clusters of or genes each In the tandemly duplicated clusters with genes, the expression patterns of of the genes were found almost identical However, in the tandemly duplicated...

Ngày tải lên: 11/08/2014, 11:21

... Expression of multiple cftr/Cftr isoforms in the gills of P annectens in freshwater…………………………………… 4.2.11 154 Cftr isoforms and polymorphism in lungs and gills of P annectens—a clue to the huge genome of ... variant or isoform…… 207 xii Lists of Figures Fig The cDNA nucleotide sequence and the translated amino acid sequence of the coding region of the predominant form (isoform 1) of cftr and Cftr, respectively, ... sequence of the predominant form (isoform 1) of Cftr from the lung of Protopterus annectens and those of other animal species obtained from Genbank Sequences are arranged in descending order of similarities...

Ngày tải lên: 09/09/2015, 17:55

... RT-PCR and cloning for gs partial fragments…………… 4.1.2 RACE PCR and cloning of RACE products……………… 4.1.3 Analyses of gs and the deduced Gs isoforms…………… 4.1.4 The phylogenetic analysis of Gs isoforms………………… ... of gs1, gs2 and gs3 in M albus……………… 4.2.3 Phylogeny and conservation of Gs isoforms in M albus… 4.2.4 The Gs isoforms, Gs1, Gs2 and Gs3 are cytosolic enzymes 4.2.5 Differential expressions of ... sequence and the corresponding deduced amino acid sequence of the complete CDS of glutamine synthetase (A) isoform (gs1) from the intestine, (B) isoform (gs2) and (C) isoform (gs3) from the liver of...

Ngày tải lên: 13/10/2015, 15:54

... contribution of this strategy to the development of plants with biofarming potential in the frame of a high demand of natural and ecologically accepted sources of renewable compounds and materials ... the evolution of vascular plants The case of Fld and Cyt c6 shows that reintroduction of these genes in the proper subcellular compartment of model and crop plants restored some of the selective ... extract and MeJA in cultured G glabra cells, whereas the mRNA levels of two SQSs and bAS were coordinately downregulated by yeast extract in these cells (Fig 5.8) The opposite effects of MeJA and...

Ngày tải lên: 25/10/2013, 05:20

... 243 11.1 Histology, etiology and clinical behavior of Wilms tumors 244 11.2 Genetics of Wilms tumors and the WT1 gene 246 11.3 Epigenetics of Wilms tumors and the ‘WT2’ locus 250 ... cancers consist of cells which resemble precursor cells of their tissue of origin and have not embarked on the normal course of differentiation, whereas others show properties of cells at intermediate ... steps and often substantial rebuilding of the tissue structure by the tumor cells, by other cells in the tissue responding to signals from the tumor cells, and by immune and inflammatory cells...

Ngày tải lên: 08/04/2014, 12:51

... consisting of the extracellular portions of TM3, TM4 and TM6 and the second and third extracellular loops of the receptor The middle region of hPTH(1-34) is sandwiched between the first extracellular ... Department of Research and Development BIOBASE GmbH Wolfenbüttel, Germany Armen H Tashjian, Jr Department of Cancer Cell Biology Harvard School of Public Health and Department of Biological Chemistry and ... consists of hydrophobic interactions (residues Leu24, Trp23 and Leu28 of hPTH(1-34), and Phe173, Leu174 of the receptor), and hydrophilic interactions between Arg20 of hPTH(1-34) and Glu180, Glu177 of...

Ngày tải lên: 08/04/2014, 12:52

... early studies of our group and others, the transcriptional regulatory sequences of the H19 and IGF2 genes emerged as candidates for cancer targeted therapy H19 and IGF2 (the human P3 and P4 promoters) ... negligible in all of the cell lines All experiments were done in triplicates and the results expressed as mean and standard error In vitro targeted therapy The cells were cotransfected with μg of the LucSV40 ... control of IGF2-P4 and H19 regulatory sequences To evaluate the possible use of IGF2-P4 and H19 regulatory sequences for targeted therapy of bladder cancer, we determined the expression of IGF2-P4 and...

Ngày tải lên: 18/06/2014, 16:20

... disappointing and most women will die as a result of their disease Success of traditional chemotherapy has been limited by drug resistance and lack of specificity to mechanisms of disease formation and ... normalization of luc activity In vitro activity and specificity of the regulatory sequences (cell killing assay) Cells were cotransfected using μg of the reporter vector Luc-4 and the indicated amounts of ... thirds of the cells and (+3) more than two thirds of cells (I-indicates the intensity of the signal, Q-indicates the quantity of signal) Some samples could not be analyzed due to lack of material...

Ngày tải lên: 18/06/2014, 15:20

... there are also reports of the presence of replicating virus in blood cells (peripheral blood mononuclear cells) and in the small and large intestine [11,16] Alternative modes of transmission, such ... Progress in cell biology of SARS-CoV: Signaling pathways Successful viral replication depends upon the ability of the virus to subvert cellular processes to their advantage and counteract cellular ... types and entry pathways, differ in their cellular exploitation mechanisms The mechanism of SARS virus pathogenesis in vivo may reflect both the effect of viral replication in target cells and...

Ngày tải lên: 19/06/2014, 08:20

... there are also reports of the presence of replicating virus in blood cells (peripheral blood mononuclear cells) and in the small and large intestine [11,16] Alternative modes of transmission, such ... Progress in cell biology of SARS-CoV: Signaling pathways Successful viral replication depends upon the ability of the virus to subvert cellular processes to their advantage and counteract cellular ... types and entry pathways, differ in their cellular exploitation mechanisms The mechanism of SARS virus pathogenesis in vivo may reflect both the effect of viral replication in target cells and...

Ngày tải lên: 20/06/2014, 04:20

... thin filaments of muscle and of the microfilaments found in practically all eukaryotic cells – it comprises 5–10% of the protein of such cells (see actin filament) In solutions of low ionic strength, ... those of The International Union of Pure and Applied Chemistry and The International Union of Biochemistry and Molecular Biology) as a means of preserving order and facilitating communication between ... member of a series of oligonucleotides of the general formula pa A[2′p5′A]n, where p and A are phosphoric and adenosine residues, respectively, and a and n are small integers (a = 1, 2, or and n...

Ngày tải lên: 23/01/2014, 07:20

... (Invitrogen) Briefly, a mixture of lg of the plasmid DNA and lL of Lipofectamine in mL of antibiotic- and serum-free DMEM was placed on cells at 30–40% confluence and incubated for h at 37 °C After ... total number of cells per wing and a reduction in the area of individual cells [8] If indeed ER is involved in the cell growth control a mutation in ER could add to the effects of an r mutation ... different cell lines, HeLa, human epithelial cells, and NIH ⁄ 3T3, murine fibroblasts Visualization of the fluorescent chimera proteins, ER, isoform ⁄ a of PDIP46 ⁄ SKAR or isoform ⁄ b of PDIP46...

Ngày tải lên: 19/02/2014, 05:20

... A10.2-treated cells; lane 6, Glu-Pg- and mAb 34D3-treated cells Bands in lanes and correspond to forms I and II of plasmin(ogen) High molecular weight bands in lanes and correspond to mAb A10.2- and mAb ... cytospun cells (F) Cells incubated with Glu-Pg and 0.5 lM of mAb 34D3 No cells were recovered after cytospining the supernatant of control and Glu-Pg ⁄ mAb 34D3-treated cells *P < 0.0001 vs control cell- bound ... calculation of mAb concentrations that produce 50% of the inhibitory effect, IC50, on, respectively, cells and fibrin: A10.2 ¼ 9.2 nM and nM; 34D3 ¼ 13.1 nM and nM; mAb mix ¼ 11.8 nM and nM K4-LBS...

Ngày tải lên: 20/02/2014, 01:20

... characterization of ANKHD1 splice variant A B Fig Expression of VBARP isoforms in vitro and in vivo: (A) In vitro transcription ⁄ translation of VBARP-L and VBARP-S One microgram of VBARP-L, VBARP-S and vector ... distribution of VBARP is independent of the cycling stage of the cells Identification of biological function(s) of VBARP using a siRNA assay To examine the role of endogenous VBARP in the regulation of ... tested cell lines and this siRNA was used in subsequent assays Following transfection of VBARP siRNA into HeLa and NT2 cells, physical observation revealed that cell death occurred in a dose- and cell- dependent...

Ngày tải lên: 07/03/2014, 21:20

... fixation of nucleus and maintenance of cell morphology No expression of CK18 or CK8 was found in noncancerous squamous epithelium CK18 and CK8 were found of 42.9% and 40.5% positive 11 Molecular Biology ... Short survival and disappointing prognosis of EC is due to its resistance to many clinical therapies such as chemotherapy and radiotherapy 10 Esophageal Cancer – Cell and Molecular Biology, Biomarkers, ... methylation and loss of RARβ2 expression was found in ESCC DNA methylation of RARβ2 and tumor grade were correlated significantly in EC And the correlation of methylation and loss of RARβ2 expression...

Ngày tải lên: 08/03/2014, 00:20

... of cell shape and mobility  ancor for other cellular structures Presentation: DNA Cell Cycle and Cell Division Some Eukaryotic Cell- Cycle Times Cell Type Cell- Cycle Times Early frog embryo cells ... Lesson 1: Basics of molecular biology I The Cell s Organization II Cell Cycle and Cell Division III Cellular Molecules IV The Genetic Dogma The Cell s Organization EUKARYOTES PROKARYOTES ... Chloroplasts (in plant cells) ATP synthesis and carbon fixation by photosynthesis Peroxisomes oxidation of toxic molecules Compartimentation of the eukaryote cell: various organelles Cell nucleus Contains...

Ngày tải lên: 13/03/2014, 19:46

... properties and molecular biology of xylanases, genetics of microorganisms producing xylanases and applications Xylan, one of the major components of hemicelluloses found in plant cell wall is ... 112, 1995 49 Hazlewood, G P and Gilbert, H J., Molecular biology of hemicellulases, in Hemicelluloses and Hemicellulases, Coughlan, M P and Hazlewood, G P Eds., Portland Press, London, 1993, 103 ... (A and B) constituted mostly by antiparrellel β strands and one short α helix and resembles a partly closed right hand.85 The differences in catalytic activities of endoxylanases of family 10 and...

Ngày tải lên: 13/03/2014, 21:56

... changes in cell structure, decreased cell adhesion, and production of new enzymes These heritable changes allow the cell and its progeny to divide and grow, even in the presence of normal cells that ... the presence of some types of cancer Chemoimmunotherapy Chemotherapy combined with immunotherapy Chemotherapy uses different drugs to kill or slow the growth of cancer cells; immunotherapy uses ... enough cells to fill the gap This characteristic is lost in cancer cells, which continue to grow after they touch other cells, causing a large mass of cells to form • Normal cells age and die, and...

Ngày tải lên: 29/03/2014, 01:20