4 involvement of the joints in diseases of the spine

... functional illiterates The main implications of these findings are that in order to use PedsQL™ in our public health setting, availability of trained interviewers during questionnaire administration needs ... complete the instrument Steps to expand the use of the Argentinean Spanish PedsQL™ 4.0 include an alternative approach to scoring for the 2–4 year-olds, further understanding of how to increase ... provide initial evidence towards the reliability and validity of the Argentinean Spanish version of the PedsQL™ 4.0 Generic Core Scales in the public health research setting The Argentinean Spanish...

Ngày tải lên: 18/06/2014, 19:20

... contain the entire coding region Thus, use of alternative promoters in DNASE1 results in no generation of protein isoforms The 5¢-UTR of eukaryotic mRNA in uences the initiation step of protein ... the cycle number at which the fluorescence of the reporter dye crossed the threshold in log-linear range of PCR The copy numbers of the respective DNASE1 cDNA were quantified by interpolating the ... corresponding to the DNASE1 cDNAs starting from either exon 1a or exon 1, respectively, into COS-7 cells and then determined the levels of DNase I activity secreted into the medium of the cells...

Ngày tải lên: 19/02/2014, 06:20

... for the binding of CP12 The presence of this region at the interface of GAPDH and CP12 could also explain the kinetic changes observed for the binding of the substrate when the wild-type recombinant ... molecular modelling of all mutant GAPDHs indicate no changes of the global structure of the mutants To test whether the interactions of these mutants with the other partners of the GAPDHCP12PRK ... partners of this complex These mutageneses are a rst step toward the understanding of proteinprotein interactions in the GAPDHCP12PRK complex and the nature of the physico-chemical forces involved in...

Ngày tải lên: 19/02/2014, 16:20

... factors including Ets, GATA, and MyoD/E-box binding factors The introduction of mutation into one of the putative Etsbinding sequences suppressed the promoter activity In addition, the specific binding ... assay (EMSA) using the Ets consensus site at )133 As the involvement of the Ets consensus binding site at )133 in the promoter activity of the mouse a3 integrin gene was suggested by the luciferase ... triplicate, and the error bars indicate the standard deviation attempted to introduce mutations into these sequences As shown in Fig 8, the introduction of mutation into one of the Ets-binding sequences...

Ngày tải lên: 21/02/2014, 03:20

... Chí Minh Tài liệu tiếng Anh Appellbaum S., Garada J and Mishra J.K., 2002 Growth And Survival Of The White Leg Shrimp (Litopenaeus Vannamei) Reared Intensively In The Brackish Water Of The Israeli ... vannamei The World Aquaculture Siciety The Oceanic Institute Briggs, M., S Funge-Smith, R Subasinghe and Phillips, M 2004 Introduction and movement of Penaeus vannamei and Penaeus stylirostris in Asia ... Litopenaeus vannamei, to Inland, Low-Salinity Waters SRAC Publication No 2601 Dall W., Hill B.J., Rothlisberg P.C., Staples D.J., 1990 The Biology of the Penaeidae Advances in marine biology, vol 27...

Ngày tải lên: 07/03/2014, 01:33

... involved in the biosynthesis of natural polyamines (putrescine, spermidine and spermine) through conversion of l-arginine into l-ornithine [16] The latter is in turn used by ODC to produce putrescine, ... iodide (PI) staining of apoptotic cells was quantified in VSVinfected Jurkat T cells in the absence or presence of spermine As shown in Fig 7, the presence of spermine during VSV infection strongly ... mitochondrial enzyme involved in the polyamine synthesis pathway through the catalysis of l-ornithine production from l-arginine Of the natural polyamines, spermine and to a lesser extent spermidine, possess...

Ngày tải lên: 07/03/2014, 21:20

... fractionated close to the top of the gradient, suggesting the low-density nature of the AQP-2-containing vesicles On the other hand, P-LAP fractionated differently and was recovered mainly in the high-density ... These results indicate that the binding of the hormone to V2 receptors on the basolateral membrane of principal cells stimulates cAMP synthesis, which leads to the translocation of AQP-2 to the ... understand the role of P-LAP in the kidney, further studies are required to determine the actual movement of the vesicles containing P-LAP employing more distinctive renal cells such as the primary...

Ngày tải lên: 08/03/2014, 02:20

... hybridizes to the center of the input target RNA The asterisk indicates the input target RNA; the arrow indicates the digested product bands The amount of digested product obtained is indicated at the ... freezing or diluting of the extracts The extracts used in one experiment were always isolated at the same time and treated in the same way So within one experiment, the ratio of the extracts of ... only in in-gel assays, the class II activity of RNH(35) only in liquid assays, whereas the class II activity of RNH(70) was detected in neither assay In order to determine the contribution of the...

Ngày tải lên: 08/03/2014, 10:20

... things during the period Few minutes before finishing speaking lesson, they prefer doing something funny or individual instead of discussing again Write-it-up goes down from 82% to 70% while the ... doing in speaking lessons  Third, more information needed for the findings of the survey will be gathered from formal and informal interview with the surveyed teachers and students Design of the ... within the scope of the study is conducted in Minh Thanh 31 secondary school The main aim of the study is to collect and analyze data regarding the recognition and utilization of speaking The...

Ngày tải lên: 15/03/2014, 10:03

... Fig Fine mapping of the putative silencing element in the first intron of the hGnRH II gene The series of mutational constructs (A) were cotransfected (1 lg each) with 0.5 pSV-b-gal vector into ... the RAR family are involved in the DNA–protein Complex in TE671 cells Intriguingly, along with the abolition of Complex A formation by RAR-specific antibody, there was a concomitant increase in ... activation [20–22] In the context of the GnRH II promoter, there is a cluster of enhancing elements, including a functional CRE, within 200 bp upstream of the hGIISil site The enhancers have...

Ngày tải lên: 16/03/2014, 10:20

... form Initially, determination of the amino-terminal amino acid sequence of purified protein was attempted, but no information was obtained, probably due to the protein modification Therefore, the ... because of the attachment of the hexahistidine, the recombinant PpTGase protein appeared to be slightly larger These results indicate that the cDNA obtained covered the entire coding region Involvement ... terminus that is required for binding to the plasma membrane, thereby being involved in the formation of the corneum [47] Additional amino-terminal residues of TGase include the sequences that are post-translationally...

Ngày tải lên: 17/03/2014, 23:20

... be flavinylated either in the WTHA strain or in the flx1D-HA strain These experiments also showed that the availability and attachment of flavin cofactors are not involved in the regulation of Sdh1p ... 5¢-UTR of methionine synthetase mRNA [46] Sequence analysis of the 5¢-UTR of this mRNA also reveals the presence of two upstream ORFs involved in regulating the translational efficiency of the main ... for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 55 Laemmli UK (1970) Cleavage of structural proteins during the...

Ngày tải lên: 23/03/2014, 07:20

... mentioning that the involvement of PKCa in these experiments has been ruled out because of the lack of inhibition by Go6976, which is a ¨ specific inhibitor of Ca2+-dependent isoforms In our hands the ... data in the literature that indicate PKCe as the only protein kinase isoform involved in the signalling pathway downstream of muscarinic m3 receptors in SK-N-BE(C) neuroblastoma cells [15] The ... processing of APP in these cells In summary, the results indicate that PKCa and PKCe have differential roles in the regulation of APP processing and sAPPa release in SH-SY5Y cells – the former being...

Ngày tải lên: 23/03/2014, 13:20

... neurogenesis The list of these genes is reported in additional file These results support the hypothesis of a possible role of E6 and E7 in the induction of neuroendocrine differentiation of SCLC ... Evaluation of the results obtained in the two systems showed deregulation of the same pathways in human SCLC and in that induced experimentally by the E6/E7 oncoproteins of HPV16 To further highlight the ... release the E2F transcription factor, subsequently leading to progression of the cell into the S-phase [14] Furthermore, E7 binds to inhibitors of cyclin-dependent kinases (p16, p21), increasing the...

Ngày tải lên: 18/06/2014, 16:20

... http://www.virologyj.com/content/2/1/1 Inhibition of PI3K results in an increase in the speed and magnitude of RV-induced apoptosis To evaluate the role of PI3K-dependent signaling during RV infection, the effects of PI3K inhibitor ... inositol lipids at the 3' position of the inositol ring These 3'phosphoinsoitides recruit proteins such as Akt and phosphoinositide dependent kinases and (PDK1/2) to the plasma membrane via their ... 3A) This increase in speed and magnitude of RV-induced apoptosis is more strikingly observed in Fig 3B, which shows the number of dead floating cells by trypan exclusion staining in the culture...

Ngày tải lên: 18/06/2014, 22:20

... involved in the regulation of recovery rather than in the initiation of the disease In addition, CCL19 and CCL21 are expressed by endothelial cells of the BBB, and are involved in the strengthening ... of the murine model provide further insights in the characterization of CCL / CCR molecules in EAE In mice with the CCL2 transgene under the control of the lck (which directs the expression of ... significant part in the regulation of intercellular interactions in the peripheral lymphoid organs, at the BBB and in the CNS In addition to defining chemotaxis, CCL-CCR interactions are involved in TH1...

Ngày tải lên: 19/06/2014, 22:20

... impairment in order to understand the mechanism of action of betaine in the central nervous system In this study, we investigated the effects of betaine on LPS-induced memory impairment using the Y-maze ... the expression of proinflammatory molecules and increased oxidative stress in aged rat kidney [6, 7] This finding indicates that the mechanism behind the actions of betaine in the central nervous ... J, Iversen LL: Regional studies of catecholamines in the rat brain I The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain J Neurochem 1966, 13:655-669...

Ngày tải lên: 19/06/2014, 22:20

... in the sublining layer or scattered throughout the synovium up to the lining layer [20] Conversely, CCR5-positive cells were detected mainly in the lining layer and in the sublining zone of the ... extent, in the perivascular infiltrates of sublining layer and in the T and B cell aggregates These findings in synovial tissue are in line with the results of the phenotypic characterisation of SF ... aggregates and in the vascular endothelium of follicular structures In contrast, CCR5+ cells were detected mainly in the lining layer and in the sublining zone of the superficial subintima and, to...

Ngày tải lên: 09/08/2014, 06:22

... maintain chondrocyte viability in the face of catabolic changes in articular cartilage Many molecular aspects of HIF-1 signaling should be also studied to further clarify the role of HIF-1 in the ... obtained from knee joints of OA patients undergoing arthroplastic knee surgery (seven OA patients) after obtaining the patients' informed consent The characteristics of patients are summarized in ... chemical inducer of HIF-1, was added to the cells during the incubation time in normoxia or hypoxia [20] In other experiments, human chondrocytes were cultured in the presence or absence of a phosphatidylinositol...

Ngày tải lên: 09/08/2014, 06:23