4 cryopreservation and storage of the cord blood unit
Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
Ngày tải lên :
12/08/2014, 11:23
... μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness of the right ear and the left ear, divided by the thickness of ... of iNOS and COX-2 in inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The ... μg of CII in the right ear and vehicle in the left ear Delayed-type hypersensitivity responses were measured as the percentage of swelling (100 × [(thickness of the right ear – thickness of the...
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IN VIVO EX VIVO OSTEOGENESIS OF HUMAN EMBRYONIC STEM CELLS
Ngày tải lên :
09/10/2015, 11:24
... extremities of mandible and the base of the skull First there is condensation of mesenchymal cells Chondrocytes differentiate from these condensed mesenchymal cells to form cartilage The cartilage then ... did not show the implanted cells within the scaffolds (Fig 7) The empty scaffolds contained few blood vessels within the pores of the scaffolds A possible explanation for the loss of the implanted ... between them and the bone surface Another feature of osteoclasts is a proton pump associated with the ruffled border that pumps hydrogen ions into the sealed compartment The sequence of resorption...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, the expression levels of let-7b and ... coefficients between the expression levels of hES upregulated miRNAs and the levels of their predicted targets The predicted targets for each miRNA were downloaded from miRNAMap2.0 [44] and their expression ... green I6, H9 and BG01v are names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in...
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báo cáo khoa học: " MicroRNAs involved in neoplastic transformation of liver cancer stem cells" pot
Ngày tải lên :
10/08/2014, 10:20
... Analysis of stem cell marker expression on the surfaces of SP and non-SP cells The number within each histogram represents the percentage of CD90.1 positive cells (I-K) Quantitative analysis of AFP and ... groups of SP cells was isolated using TRIZOL reagent (Invitrogen) according to the instructions of the supplier and was further purified using an RNeasy mini kit (Qiagen, Valencia, CA USA) The miRCURY ... results of the study, in order to verify the formation of HCC Cell isolation and primary culture Fetal liver cells were obtained from embryonic day 14 rat fetuses by the procedure of Nierhoff et...
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Báo cáo y học: " In-vivo kinetics of inhaled 5-Aminolevulinic acid-Induced Protoporphyrin IX fluorescence in bronchial tissue" pdf
Ngày tải lên :
12/08/2014, 15:20
... at 635 nm Within areas of positive PPIX-fluorescence the tip of the bronchoscope was directed towards the center of the lesion, and only the central spot was used for spectroscopic analysis Spectral ... application of scattered light at 840 nm, which is reflected from the bronchial tissue The quantity of porphyrin fluorescence can be calculated by the relation between the intensity of autofluorescence, ... wavelengths of 380–440 nm Spectra have been normalized to the remission peak at 840 nm The spectral quantities of PPIX fluorescence according to equation 1, the visual ratings and the corresponding...
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Báo cáo khoa học: "Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains" pptx
Ngày tải lên :
12/08/2014, 18:22
... animals 628 and 192, the lack of IgA detection could be due to the sensitivity of the ELISA test available In the case of animal 191, as discussed above, the reason could have been the lack of infection ... further analyze the cell-to-cell spread of the N569 and A663 strains, the infection plaque size assay was performed This assay takes into consideration the cells lysed by the virus and also the ... each other during the course of the study in order to avoid viral spread and contamination Animal care and experimental procedures were performed in accordance with the requirements of the National...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
Ngày tải lên :
23/04/2013, 21:38
... of PA/ EPI in the plasma was 15.854 h, 1.9-times that of free EPI Altogether, PA/EPI had the longer t1/2 and MRT, lower Vd and CL than the free drug in rats At the same time, the AUC of EPI and ... the stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and ... NMR, and then PANs with moderate size and low potential were prepared by the solvent diffusion method The storage stability of PANs was studied in the aqueous medium, and the acute toxicity of PANs...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the cell extract ... anti-GroEL Ig [27] The high yield of the UP12-GroEL complex isolation and the ATP-mediated dissociation of the complex strongly support the suggestion that the two stress proteins GroEL and UP12 interact ... acrylamide in the separating and stacking gels, respectively In order to estimate the ratio between the amounts of UP12 and the total amount of protein in the extracts, a series of samples containing...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
Ngày tải lên :
06/03/2014, 11:20
... in the presence of lm Ga-PP, and in parallel in the absence of any porphyrin compound and in the presence of lm Fe-PP, respectively, as controls The cytoplasmic fraction was isolated from the ... indicated (A) The Soret band region (380–470 nm) (B) The region from 500 to 700 nm (C) Spectra of catalases produced in the presence of Cu-PP, Mg-PP, and PP The intensities of these spectra have ... further characterize and analyse the amount of Fig Fluorescence emission spectra of PP, Mg-PP, Cu-PP, and Zn-PP, and of the porphyrins contained in isolated PP-KatA, Mg-PPKatA, Cu-PP-KatA, and...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
Ngày tải lên :
07/03/2014, 16:20
... as the binding constants and the extent of autoregulation The regulatory proteins may reside either in the nucleus or in the cytoplasm or in both, thus controlling the network The existence of ... indicates the number of binding sites and p refers to the protein Because Gal80p and Gal3p are also regulated by the GAL system, their total concentrations are dependent on the status of the switch ... into the importance of their roles in the overall performance of the network A similar experimental evaluation of the roles of individual elementary mechanisms in vivo is very difficult and tedious...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... from the administered [75Se]Der p2, indicate together with its own remaining intact levels (the 16 kDa band) that the clearance and further metabolism of the allergen was altered as a result of the ... airways of Der p 2-sensitized and nonsensitized mice The fate of the allergen could be followed in vivo both at the whole-body level and at the molecular level, through the application of a newly ... conclude that the inflammatory state of the lung influence on the rate of metabolism and clearance of Der p Thus, an allergic response may lead to prolonged retention of the allergen in the airways...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... topology where the loop is in the thylakoid lumen, and the N -and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal loop, like that of PSI-K, ... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the transformed knock-out line does not reach the level of the ... integration of the subunit into the PSI complex A protein band smaller that the tagged versions of PSI-G is present in the lanes with the loop-tagged PSI, but is also seen faintly in the lanes with the...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
Ngày tải lên :
08/03/2014, 08:20
... A-deficient rats In the liver of rats with hypervitaminosis the results showed a decrease in the amount of p53 and an increase of the c-Jun The figure shows that the expression of p53 is time course ... region of the c-Jun promoter that expands between the start transcription site and the )504 position Statistics In the experiment shown in the Table 1, a two-way ANOVA was performed; in the other ... performed The homogeneity of the variances was analyzed by the Levene test; in those cases in which the variances were unequal, the data were adequately transformed before the ANOVA The null hypothesis...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
Ngày tải lên :
08/03/2014, 22:20
... effects of EBI compounds on the plasma membrane H+-ATPase of U maydis Furthermore, the utilization of two different targets in the same biosynthetic route permitted us not only the confirmation of the ... reports, the joint use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the influence of direct effects, i.e sterol alteration, and indirect ... this case, the enzyme is inhibited by the absence and not by the presence of the activator (A) If KSs ¼ KSm and KAs ¼ KAm and ¼ k < k¢, we have noncompetitive activation, in which the observed...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
Ngày tải lên :
16/03/2014, 01:20
... help understand the developmental process, in the development of cancer biomarkers and to evaluate the therapeutic effects, in terms of cancer therapy, in medicine Here, we review the currently ... a better understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms ... pre-miRNA by Dicer The synthetic duplex form of premiRNAs carrying quencher and organic dye at each strand can be designed In the presence of the Dicer enzyme, the quencher molecule and fluorophore...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... Laboratories) The procedure was made quantitative by the use of known amounts of proteins submitted to SDS-PAGE and staining with the appropriate antibody The bands were then scanned, and the areas of the ... brain and aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform, ... completely prevented (Fig 3B) by the administration to the animals of synthetic calpain inhibitor-1 (C.I.1) [33,34] Digestion of HSP90 and NOS in brain and aorta of normotensive and hypertensive rats treated...
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báo cáo hóa học:" In vivo properties of the proangiogenic peptide QK" pdf
Ngày tải lên :
18/06/2014, 15:20
... samples of the ischemic and non-ischemic HL were collected and frozen with liquid nitrogen and then were homogenized and digested; the microspheres were collected and suspended in N,N-dimethylthioformamide ... published by the National Institutes of Health in the United States (NIH Publication No 85-23, revised 1996) and approved by the Ethics Committee for the Use of Animals in Research of "Federico ... http://www.translational-medicine.com/content/7/1/41 Figure Diagram 3of the kinetics of wound closure (A) Diagram of the kinetics of wound closure (A) VEGF165 and QK accelerate the closure of full thickness punch biopsy wounds...
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Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor" potx
Ngày tải lên :
18/06/2014, 16:20
... to the logarithm of the initial amount of the target DNA in the sample The relative concentration of one target with respect to another is reflected in the difference of the cycle number, the ... sample) The SD of ΔΔCt is the same as the SD of the ΔCt The ΔCt values of both the tumor and normal samples are standardized to HGPRT used as endogenous control The values defined as increase of expression ... point of view very attractive, the analysis of the correlation of the expression levels of the two forms L and I in the tumor samples not support it at present Decrease in the level of the L...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
Ngày tải lên :
20/06/2014, 01:20
... distances between the centroids of the digitized attachment areas on talus, calcaneus and fibula Once the position of the AJC was reproduced in the modeling software, the length of the ATFL and CFL was ... position of AJC motion There was no bony interference with the path of the ATFL and CFL The position of the AJC during MR scanning was defined as the neutral reference position The length of the ATFL ... in-vivo behavior of these two ligaments is still not well studied To the best of our knowledge, no data has been reported on the elongation of the ATFL and CFL in-vivo The objective of the current...
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