4 cells used in cartilage repair and tissue engineering

... as a coordinate partner to insulin by reducing food intake, slowing gastric empty and inhibiting digestive secretion in short term Due to the release of insulin, C-peptide and amylin, -cells ... and Ins2 in rodents) and synthesis of insulin is achieved by bio-processing from its precursors, preproinsulin and proinsulin, which ensures the production of sufficient amounts of mature insulin ... of CNC and the small Maf proteins (87-90) In contrast to the small Mafs, the large Maf proteins including MafA, c-Maf, MafB and Nrl contain a transactivator domain in their amino terminus and activate...

Ngày tải lên: 11/09/2015, 09:58

... expression of insulin and proinsulin in stage IV cells Quantification of IHC staining shows FN and LAM increases the percentage of (B) proinsulin, and (C) insulin positive cells (mean ± S.D.; ** indicates ... insulin and Glut2 gene expressions, proinsulin and insulin protein levels, accumulation of cytoplasmic granules including a, b, and δ granules, and insulin release in response to elevated glucose concentration ... LC: Distinct distribution of laminin and its integrin receptors in the pancreas J Histochem Cytochem 2002, 50(12):1625-1632 Hulinsky I, Harrington J, Cooney S, Silink M: Insulin secretion and DNA...

Ngày tải lên: 10/08/2014, 05:21

... 6:42 line of defence against a pathogen attack and rapidly activate defence signalling pathways following initial infection TLRs are considered as playing a crucial role in the switch from innate ... situation prevailing in the presence of TLR2 ligand TLR7 and triggering resulted in little impact on IκBα, which is not surprising considering the reported low expression levels of TLR7 and in IM-MDDCs ... Actin Flagellin R837 15 30 Unmethylated DNA 15 30 15 30 I B -P I B Actin NF-κB is activated in IM-MDDCs following TLR2, and Figure triggering NF-κB is activated in IM-MDDCs following TLR2, and...

Ngày tải lên: 12/08/2014, 23:20

... venous blood and supplied by the portal vein that drains the blood from the intestinal system (including the pancreas and the spleen), rich in nutrients and poor in oxygen The remaining blood supply ... molecules or by mediating intracellular signal through interaction with integrins β1-integrin is a component of receptor for ECM proteins laminins and collagens The β1-integrin knockout embryonic ... foregut and invade as cords into the surrounding septum transversum mesenchyme (Douarin, 1975; Medlock and Haar, 1983) The hepatoblast cords intermingle with the vitelline veins, anastomosing into...

Ngày tải lên: 11/09/2015, 16:06

... protein Frequently, epitopes are masked because of the folding properties of a protein in vivo or because of pre-existing protein-protein interactions that may compete for antibody binding To ... activity and used for the infection of LuSIV indicator cells Relative virus infectivity was determined by comparing the Tat-dependent expression of luciferase in the target cells (Fig 7) Interestingly, ... initial findings and suggest that – instead of inducing APOBEC3G degradation – Vif induces conformational changes in APOBEC3G that affect the ability of antibodies to interact with the protein (manuscript...

Ngày tải lên: 13/08/2014, 13:20

... reviewed in (68) and compared in (69) Currently, there are a number of widely used insulinsecreting cell lines These includes RIN (70), HIT (71), BRIN-BD11 (72), MIN6 (73) and INS-1 cells (74) In 1992, ... involved in mediating the action of Rac1 on actin cytoskeleton remodeling In this thesis study, their potential roles in insulin secretion and cell survival were investigated in INS-1 cell line, ... differentiated insulinsecreting cell line, INS-1, from cells isolated from X-ray-induced rat insulinoma (74) INS-1 cells, containing the highest insulin content (around µg/106cells, corresponding to...

Ngày tải lên: 14/09/2015, 14:08

... HAP1 and insulin HAP1 is also expressed in INS-1 cell line, a commonly -used insulin-secreting cell line from rat insulinoma Western blotting further confirms that there are two HAP1 isoforms in INS-1 ... produce insulin by themselves, thus have to inject insulin substitute to maintain glucose homeostasis On the other hand, type patients result from relative insulin deficiency and insulin resistance, ... of insulin-secreting cell lines have been created by induced insulinomas, viral transformation, and transgenic mice [34] Among them, the INS-1 cell line from rat insulinoma is a most widelyused...

Ngày tải lên: 12/10/2015, 17:35

... follows: aprotinin, leupeptin, pepstatin A, AEBSF and proteinase inhibitor mixture that contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A from Wako Pure Chemical Industries (Osaka, ... (N-(R)(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-l3-(2¢-naphthyl)alaninyl-l-alanine 2-aminoethyl amide; TAPI-1) did not affect the processing A commercially available proteinase inhibitor mixture containing AEBSF, aprotinin and some other inhibitors inhibited ... [4,5,8] and stimulates cell growth in culture medium containing insulin or IGFs [9,10] The IGFBP-rP1 mRNA is expressed in a wide range of normal tissues including the heart, spleen, ovary, small intestine...

Ngày tải lên: 19/02/2014, 07:20

... with amino acids added) containing 2% D-glucose, and the cultures diluted to an D600 of 0.5 in synthetic medium containing 0.5% D-glucose and grown further with shaking at 30 °C Upon reaching an ... to a ®lter containing soluble protein extracts obtained from wild-type and mls1D cells that were propagated in rich medium supplemented with ethanol This resulted in a protein band with a molecular ... compartment in cells grown on glucose, Mls1p was selectively retained in the cytosol of cells propagated on ethanol Interestingly, the C-termini of both Mls1p and Aat2p contain acidic amino-acid...

Ngày tải lên: 22/02/2014, 04:20

... protease and protein phosphatase inhibitors, frozen within 10 s, and thawed in boiling water to minimize postincubation signalling modifications in the cells and protein modifications during immunoprecipitation ... serine ⁄ threonine phosphorylation of IRS-1 and IRS-2 inhibits their binding to the juxtamembrane region of the insulin receptor and impairs their ability to undergo insulin-induced tyrosine ... were incubated with indicated concentration of insulin for 10 Mean ± SE, n ¼ subjects In this and the following figures, the insulin-stimulated effect was obtained by setting the value with no insulin...

Ngày tải lên: 07/03/2014, 16:20

... IGF2R-binding site, while the B domain and the ®rst A domain helix contain the major determinants for IGFBP binding In this study we show, using IGF-I, IGF-II and 14 IGF analogues, that potency in ... stimulate DNA synthesis in L7 murine ®broblasts compared with IGF-I [14] Interestingly, strong correlations between insulin or insulin mutant binding to the insulin receptor and metabolic potencies ... rhIGF1R binding by IGF-I, IGF-II and 14 analogues using BIAcore analysis IGF1R binding by all of these analogues has not previously been compared in a single binding assay Analogues included in this...

Ngày tải lên: 08/03/2014, 22:20

... eumelanins, and the reddish to yellow, sulfurcontaining phaeomelanins [17] Although both types of melanins are found in different relative proportions in all skin types, the phaeomelanin/eumelanin ... loading and transfer were ascertained by cutting the lower portion of the membrane before blocking and staining for total protein with Amido Black RESULTS AND DISCUSSION Seven HMC lines and three ... the Gs protein In many GPCRs, the third intracellular loop and the cytoplasmic C-terminal extension are the main regions involved in coupling to the G proteins [2] It is clear that in the case...

Ngày tải lên: 17/03/2014, 10:20

... 30] and fixed in 3% (w/v) paraformaldehyde for 30 at room temperature After blocking in bovine serum albumin and gelatine membranes were incubated with rabbit antiIRS1, caveolin, or insulin receptor ... by centrifuging through dinonylphtalate and freezing in an aluminium block kept at )20 °C Non-specific uptake was determined in the presence of 25 lM cytochalasin B by terminating the incubation ... receptors After incubating cells with a submaximal concentration of insulin the insulin-induced increase in receptor autophosphorylation was comparable in the isolated caveolae fraction and in the total...

Ngày tải lên: 23/03/2014, 12:20

... protease inhibitor cocktail for mammalian extracts (containing AEBSF, aprotinin, leupeptin, bestatin, pepstatin a and E-64) was obtained from Sigma (St Louis, MO) Plasmid construction and site-directed ... remain uncapped and therefore can be translated only by a cap-independent mechanism In the HCV IRES-containing constructs, initiation of transla- nt 342 ATG initiator in core ORF CMV del ATG initiator ... Frameshifting is mediated by slippage of ribosomes during translation elongation at core codons 8–11 and yields a core+1 chimeric protein containing the first 8–11 amino acids of core fused to amino...

Ngày tải lên: 30/03/2014, 03:20

... vascular stages during hepatic colon carcinoma metastasis Materials and methods Cell line and maintenance Murine colon carcinoma cell line (CT26) was obtained from American Tissue Culture Collection ... lipoprotein, ovalbumin); negative phagocytosis (1 μm latex particles) and CD45 antigen expression; positive lectin binding-site expression (wheat germ and viscum album agglutinins); and negative ... Eto S: CD44 stimulation induces integrin-mediated adhesion of colon cancer cell lines to endothelial cells by upregulation of integrins and c-Met and activation of integrins Cancer Res 1999, 59:4427-34...

Ngày tải lên: 18/06/2014, 15:20

... Scotlandi K: Inhibition of insulin-like growth factor I receptor increases the antitumor activity of doxorubicin and vincristine against Ewing’s sarcoma cells Clin Cancer Res 2001, 7:1790-7 Martins ... [50] IGF1R signaling was blocked in many other types of sarcomas to explore its role in cell proliferation and survival in vitro, and tumor growth, invasion and metastasis in vivo in animal models ... overcome KIT and PDGFRA-dependent imatinib resistance [55] Glycemic derangements related to insulin-like growth factors such as the pro-IGF-IIE and insulin-like growth factor-binding proteins have...

Ngày tải lên: 18/06/2014, 16:20

... protein kinase PKR in the inhibition of varicella-zoster virus replication by beta interferon and gamma interferon J Gen Virol 2005, 86(Pt 1):1-6 Sainz BJ, Halford WP: Alpha/Beta interferon and ... DA: Trafficking and signaling pathways of nuclear localizing protein ligands and their receptors Bioessays 2004, 26(9):993-1004 Lucin P, Jonjic S, Messerle M, Polic B, Hengel H, Koszinowski UH: ... KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 infection Likewise,...

Ngày tải lên: 19/06/2014, 08:20

... protein kinase PKR in the inhibition of varicella-zoster virus replication by beta interferon and gamma interferon J Gen Virol 2005, 86(Pt 1):1-6 Sainz BJ, Halford WP: Alpha/Beta interferon and ... DA: Trafficking and signaling pathways of nuclear localizing protein ligands and their receptors Bioessays 2004, 26(9):993-1004 Lucin P, Jonjic S, Messerle M, Polic B, Hengel H, Koszinowski UH: ... KOS-GFP strain [50] caused a slowly progressing infection in rag2-/- mice that was not lethal until 25 ± days p.i Thus, Stat plays an integral role in innate resistance to HSV-1 infection Likewise,...

Ngày tải lên: 20/06/2014, 04:20

... 1996, 39:968-978 Morales TI: The insulin-like growth factor binding proteins in uncultured human cartilage Increases in insulin-like growth factor binding protein during osteoarthritis Arthritis ... small-molecule inhibitor of the binding of insulin-like growth factor (IGF)-1 to IGF-binding proteins (IGFBPs), with 125Ilabeled IGF-1 binding to cartilage labeled IGF-1 binding to cartilage Explants ... washes of minutes each in 10 mM Tris-buffered saline, pH 7.4, containing 0.15 M NaCl (TBS), membranes were incubated for 30 minutes in TBS containing 3% NP40 and blocked in TBS containing 0.5%...

Ngày tải lên: 09/08/2014, 01:23