3the electrospinning approach in cardiac tissue engineering

... (Figure 1) They include structural matrix proteins (collagens, laminin, fibronectin, fibrin, etc.) and molecules modulating cell migration (integrins), cell-cell and cell-substrate interactions ... and other defects in the lung tissues will increase along with the constantly rising number of people suffering from respiratory troubles Recent advances in airway tissue engineering provide a good ... cell; INK4a: cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); iPS: inducible pluripotent stem cell; Klf4: Krueppel-like factor 4, a key transcription factor in maintaining pluripotency;...

Ngày tải lên: 10/08/2014, 05:21

... AND CROSSLINKING OF COLLAGEN SCAFFOLDS AND THE POTENTIAL IN CORNEAL TISSUE ENGINEERING APPLICATION ZHONG SHAOPING (M.ENG INSTITUTE OF PROCESS ENGINEERING, CHINESE ACADEMIC SCIENCES, BEIJING, PRC) ... a high-risk investment, more than $3.5 billion has been invested in worldwide research and development in tissue engineering [32, 33] Tissue engineering firms have increased spending at a compound ... goals in tissue engineering fields 2.2 Scaffold Materials The first issue with regard to tissue engineering is the choice of suitable materials for scaffolding The materials for tissue engineering...

Ngày tải lên: 14/09/2015, 09:33

... the treatment of the infarcted myocardium, of which the tissue engineering approach is gaining much attention Cardiac tissue engineering promises to bring about a change in the treatment of patients ... infarcted myocardium 14 2.5 Nanofibers by electrospinning 2.5.1 Electrospinning – Basic principle Electrospinning refers to the technique of drawing very fine fibers on a nano scale by applying ... 2.4.2 Gelatin 12 2.4.3 Fibrinogen 14 2.5 Nanofibers by electrospinning 15 2.5.1 Electrospinning – Basic principle 15 2.5.2 Advantages of electrospun nanofibers for tissue engineering 17 Chapter...

Ngày tải lên: 05/10/2015, 19:04

... that restore, maintain or improve tissue function [3] By combining life sciences, engineering principles and innovation, tissue engineering holds enormous potential in confronting the supply crisis ... Introduction Scaffold-based tissue engineering Electrospinning and tissue engineering 12 Periosteum and bone healing 16 Chapter Three 19 Electrospinning ... proteins were retained in this manner indicating minimal processing damage Investigations into the performance of this novel hybrid mesh as a three-dimensional (3D), bio-functional tissue engineering...

Ngày tải lên: 14/09/2015, 08:24

... 1.2 Tissue- Engineering Scaffolds The main goal of tissue engineering is to produce new tissue where it is needed Therefore, knowledge of the structure and functional limits of the regenerated tissue ... progenitor cells in a derivatized hyaluronan-gelatin composite sponge Tissue Engineering 5, 545–554 45 Doser, M (1999) Criteria for the selection of biomaterials for tissue engineering, in Polymers ... Sambanis, A (1995) Tissue engineering: from biology to biological substitutes Tissue Engineering 1, 3–13 23 Shors, E C and Holmes, R E (1993) Porous hydroxyapatite, in An Introduction to Bioceramics...

Ngày tải lên: 10/04/2014, 11:11

... reported in Figure Discussion Cellular therapy is an emerging field in medicine; all the stem cell medicinal products must be in compliance with principles and guidelines of good manufacturing practice ... of the invasion assay as a potency testing for BM-MNC cells: we purpose to define as a minimal criteria to establish cell potency in cardiac regeneration, the obtainment of an invasion index not ... preliminary assay was performed with the aim to find the dilution where the spiked endotoxin can be detected without inhibiting or enhancing the test Once prepared, the cartridge was inserted in...

Ngày tải lên: 18/06/2014, 15:20

... both ‘cartilage’ and tissue engineering or ‘bone’ and tissue engineering were in 1991, accounting for 22% of all tissue engineering and ‘regenerative medicine’ entries Since that year, both ... characteristics and their tissue formation potential make MSCs appropriate cells for tissue engineering in AD Here, degenerated joint tissues will be substituted by MSCbased tissue engineering approaches described ... screening of antirheumatic drugs have been established [12] Joint tissue engineering: clinical applications The first entry for the key word tissue engineering , also termed ‘regenerative medicine’,...

Ngày tải lên: 09/08/2014, 01:22

... set up utilizing the human atrial cardiac tissue model for apoptosis studies inducing apoptotis just in accordance to our clinical routine with cardioplegia and reperfusion without induction of ... apoptosis (described in the following sections) Cardiac specimens were outside the body before being mounted and tested in the chamber system for a maximum of 45 min, but during the incubation time ... Japan) Only cardiac specimens consisting of ≥ 99% viable cardiomyocytes in the centre were included Due to the preparation the section margins (cutting edges) of the cardiac specimens contained 5-10%...

Ngày tải lên: 10/08/2014, 10:20

... References Anderer U, Libera J: In vitro engineering of human autogenous cartilage J Bone Miner Res 2002, 17(8):1420-1429 Hutmacher DW: Scaffolds in tissue engineering bone and cartilage Biomaterials ... are regarded as most promising in skeletal tissue engineering by some researchers A recently elaborated approach in extracorporal tissue engineering is therefore the avoidance of non-degradable ... 21(24):2529-2543 Lindenhayn K, Perka C, Spitzer R, Heilmann H, Pommerening K, Mennicke J, Sittinger M: Retention of hyaluronic acid in alginate beads: aspects for in vitro cartilage engineering J Biomed...

Ngày tải lên: 11/08/2014, 23:22

... micromass culture technology in tissue engineering Head & Face Medicine 2007:4 Springer IN, Fleiner B, Jepsen S, Acil Y: Culture of cells gained from temporomandibular joint cartilage of non-absorbable ... arthroscopic and tomographic findings Int J Oral Maxillofac Surg 1988, 17:128-133 Martin JA, Buckwalter JA: The role of chondrocyte-matrix interactions in maintaining and repairing articular cartilage ... skeletal tissue substitute is bone, cartilage plays the most prominent role in orthopaedics [10] Cartilage engineering therapies were mainly invented and tested in the orthopaedic field but are now introduced...

Ngày tải lên: 12/08/2014, 00:20

... 2.6 Bone Tissue Engineering 30 2.6.1 Four-Stage Bone Formation in Bone Tissue Engineering 30 2.6.2 Strategies in Bone Tissue Engineering .32 xi 2.6.2.1 Growth Factors Approach ... 1.4 Bone Tissue Engineering 1.4.1 Limitations in Bone Tissue Engineering 1.5 Importance of Vascularisation 1.5.1 Vascularisation in Bone Tissue Engineering 1.5.2 ... Bone Tissue Engineering, 2012 Bioreactor & Growth Environments for Tissue Engineering Training Course, 5-7 November 2012, Keele, United Kingdom FS Goh, Y Liu, SH Teoh Effect of Desferrioxamine...

Ngày tải lên: 09/09/2015, 10:16

... PHIs in tissue engineering As reviewed in chapter 1, clinical applications of engineered tissues are currently limited mainly to thin or avascular tissues due to a difficulty in stimulating adequate ... preventing the realization of this goal 1.1.2 Vascularization is a major obstacle in tissue engineering While the field of tissue engineering has come a long way since its original conception in ... fracture healing 28 2.4 Potential applications of PHIs in tissue engineering 31 Chapter Incorporation of a PHI into Scaffolds: A Vascularization Strategy for Tissue Engineering Applications...

Ngày tải lên: 09/09/2015, 11:11

... proline into the wound [78] Providing additional proline or glutamine in the diet to enhance collagen biosynthesis, however, does not result in increased collagen accumulation In contrast, arginine, ... proline and hydroxyproline As a non-essential amino acid, proline is synthesized from arginine/ornithine via the urea cycle and glutamate (directly or indirectly from glutamine via glutaminase) ... 71 Chapter Peri-implantational fibrosis: a bottleneck in tissue engineering 72 4.1 Biomaterials in tissue engineering 73 4.2 Foreign body response 73 4.3 Biocompatibility...

Ngày tải lên: 10/09/2015, 15:52

... Chapter 1: Introduction   Chapter 1: Introduction The design of biomaterials is important in the field of tissue engineering and regenerative medicine Since the development of tissue engineering scaffolds, ... and new tissue formation Hence, the properties of the scaffolding materials are crucial in determining the success of many tissue engineering applications The general tissue engineering paradigm ... of donor organs and tissues available This severe organ shortage led to the inception of Tissue Engineering Tissue Engineering is about developing new approaches to encourage tissue growth and...

Ngày tải lên: 11/09/2015, 09:10

... regenerative approach on treating the degenerating discs Thus, more emphasis 10 is being placed on tissue engineering approaches using cell -tissue based constructs that 11 can function clinically in tissue ... Buffered Saline BSA Bovine Serum Albumin DAPI 4',6-diamidino-2-phenylindole FBS Fetal Bovine Serum Abstract The aim of this study was to develop a tissue engineering approach in regenerating the ... replacement 20 Tissue Engineering Tissue engineering is based on the application of concepts in engineering and life sciences to develop biological substitutes that restore or improve tissue function...

Ngày tải lên: 11/09/2015, 09:57

... potential in promoting angiogenesis in multi-layer membrane tissue engineering Through the understanding of the challenges faced in the use of biodegradable PCL film for membrane tissue engineering, ... failure Tissue engineering has been heralded as the new wave to revolutionize the healthcare-biotechnology industry It is a multidisciplinary field and involves the integration of engineering principles, ... Significance and importance of tissue engineering 12 2.3 Poly(ε-caprolactone) thin films and matrices 16 2.4 Current progress of poly(ε-caprolactone) in biomedical engineering 17 2.5 Introduction to lasers...

Ngày tải lên: 14/09/2015, 08:36

... though much work has been done on it Tissue engineering 1.2.1 Definition Tissue engineering is an interdisciplinary field that applies principles of engineering and the life sciences toward the ... challenges in tissue engineering In recent years there has been remarkable progress in tissue engineering, not only in regeneration of nerve [31], liver, myocardium, pancreas, bone, cartilage, skin and ... In general, allogeneic MSC seeding is preferred 18 - Introduction 1.2.5 Materials for tissue engineering 1.2.5.1 Requirement for tissue engineering Tissue...

Ngày tải lên: 16/09/2015, 08:30

... look into the field of bone tissue engineering for an ideal bone substitute 1.1.3 Strategies in BTE Tissue engineering is the restoration, improvement, maintenance and substitution of damaged tissues ... a heparin binding site in N-terminal region of mature BMP-2 polypeptide In pioneering work by Ruppert in 1996, it was shown that BMP-2 activity was increased upon interactions with heparins present ... present in ECM In the presence of N terminus of BMP-2 and heparin, there is a five-fold increased in bioactivity (Ruppert et al., 1996) This lead to various studies on heparin effects on BMP-2 in mind...

Ngày tải lên: 09/10/2015, 11:18

... rather than just the repair of skeletal tissue, and this treatment strategy was later coined as “bone tissue engineering A key component in tissue engineering for bone regeneration is the scaffold ... respectively 1.1.1 Bone tissue engineering Loss of human tissues or organs is a devastating problem that can affect individuals of all ages Bone, a complex living tissue that provides internal support ... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of...

Ngày tải lên: 09/10/2015, 11:18

... the advantage of reduction in computational cost while maintaining the accuracy when applied to flow in a channel with linear or almost linear bottom/bed But indeed, in many cases, water flows ... the advantage of reduction in computational cost while maintaining the accuracy when applied to flow in a channel with linear or almost linear bottom/bed But indeed, in many cases, water flows ... to examine the effect of sidewall friction in analyzing a backwater profile in a straight rectangular channel 2.2 Depth-averaged Model in Generalized Curvilinear Coordinate System In solving these...

Ngày tải lên: 06/11/2012, 10:35