34 attractive and repulsive signaling in nerve growth cone navigation
báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
Ngày tải lên :
19/06/2014, 22:20
... forebrain cholinergic neurons [57] and direct TNFα administration in the brain of mice results in an impairment of basal forebrain cholinergic function [58] However, whether induction of iNOS and ... absence blots detecting iNOS in total NGF in A: Western blots detecting iNOS in total protein extracts from PC12 cells treated for 24 hr with increasing concentrations of NGF in the presence or ... detecting iNOS in total protein extracts from PC12 cells simultaneously treated for 24 hr with 10 ng/ml NGF and 10 ng/ml TNFα in medium containing serum, in serum free medium (SF) or in defined...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
Ngày tải lên :
14/02/2014, 19:20
... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... the structural and functional determinants of the protein–protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module in MAP2) across ... in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within the KIND2 domain (amino acids 456–620), we analyzed five EGFP-fused KIND2 domain...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
Ngày tải lên :
23/03/2014, 06:20
... target proteins in vivo The attachment of SUMO may result in diverse effects on the substrate protein, including cellular and subnuclear localization [20,21], changes in stability [22] and transcriptional ... by phosphorylation following 4b-phorbol 12-myristate 13-acetate (PMA) induction [18], and serine residues 238 and 243 in the hinge region of human LRH-1 are important in this PMA-dependent transactivation ... synthesized in vitro and incubated with recombinant SUMO-1 protein, E1 (SAE1 ⁄ SAE2) and E2 (UBC9) enzymes Upon separation of the reaction products by SDS ⁄ PAGE, a slowermigrating band at approximately...
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báo cáo hóa học: " Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury" potx
Ngày tải lên :
19/06/2014, 22:20
... pathology in Gsn-L mice, only the gene expression of proinflammatory cytokines in the brains of Gsn-H mice was determined Significant reductions in brain levels of early cytokines, including IL-1b and ... PBS for 20 and were developed by incubating in 0.025% diamino-benzidine (DAB; Sigma-Aldrich) and 0.002% H2O2 in PBS The DAB reaction was halted using PBS, followed by three 10-min PBS rinses Quantification ... serially collected in PBS and finally stored in cryoprotectant solution at -30°C Some of the brain sections were mounted on lysine-coated slides and stained with hematoxylin and eosin (H&E) Quantitative...
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Báo cáo hóa học: " Resveratrol engages AMPK to attenuate ERK and mTOR signaling in sensory neurons and inhibits incision-induced acute and chronic pain" pptx
Ngày tải lên :
21/06/2014, 19:20
... 3A) and a reversible inhibition of both ERK (Figure 3B and C) and mTOR signaling (Figure 3D–H) Hence, resveratrol dose- and time-dependently activates AMPK and inhibits ERK and mTOR signaling in ... phosphorylation of ERK and eIF4E in TG cultures (Figure 5) These findings indicate that resveratrol blocks IL6-induced signaling in sensory neurons Figure Resveratrol blocks IL-6 induced signaling in sensory ... potently and efficaciously inhibits ERK and mTOR signaling in sensory neurons in vitro The mTOR [39-42] and ERK pathways [43] have been linked to pathology in multiple preclinical pain models and...
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Báo cáo y học: "Activation of WNT and BMP signaling in adult human articular cartilage following mechanical injury" potx
Ngày tải lên :
09/08/2014, 08:22
... time point in the presence of FBS and a significant difference (p < 0.05) at the hour and day time points in the absence of serum At the protein level, FRZB was present in both injured and uninjured ... staining for FRZB protein (red) in (c) uninjured and (d) injured explants at the day time point Haematoxylin was used as a nuclear counterstain (e,f) Larger magnifications of the boxed areas in ... immunohistochemistry (h) Haematoxylin-eosin and (i) safranin O stainings of an explant with a relatively high degree of osteoarthritis (modified Mankin score 5) (j-m) Immunostaining for β catenin in parallel, non-consecutive...
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Báo cáo khoa hoc:" VEGF receptors on PC12 cells mediate transient activation of ERK1/2 and Akt: comparison of nerve growth factor and vascular endothelial growth factor" doc
Ngày tải lên :
11/08/2014, 08:20
... useful model for understanding endostatin effects on cells Figure 164 only stained cells revealed binding of the APmESXVIII) affinity PC12 (c) staining of (a, d) proliferating and mVEGF (b) differentiated ... Results in BioMedicine 2006, 5:8 http://www.jnrbm.com/content/5/1/8 lines, PC12 and CPAE cells were incubated with AP fusion proteins The AP-mVEGF164 affinity probe strongly stained proliferating and ... plates, and AP staining was performed as described in [20] Staining was monitored with a Nikon Eclipse TE2000-U inverted microscope and documented using the Spot Insight QE Color imaging software...
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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt
Ngày tải lên :
14/08/2014, 21:20
... of insulin and wingless signaling pathways in Drosophila S2 cells Figure Activation of insulin and wingless signaling pathways in Drosophila S2 cells (a) Schematic representation of the insulin ... upon activation of the insulin and wingless pathways in S2 cells Binding of insulin-like peptides to the insulin receptor in Drosophila cells leads to the activation of dPI3 kinase, which Genome ... of uridine-rich small nuclear ribonucleoprotein particles and more than 100 additional proteins [21,22] Splicing regulatory factors, including members of the Serine and Arginine-rich (SR) and heterogeneous...
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Báo cáo khoa học: "Immunohistochemical Localization of Nerve Growth Factor,Glial Fibrillary Acidic Protein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon, and Spinal Cord of Developing Mongolian Gerbil" ppsx
Ngày tải lên :
07/08/2014, 15:20
... midbrain, NGF-IR was found in the internal geniculum of facial nerve after PND (Fig 4D), observed well fine at PNW2 (Fig 1C) and the nucleus of the spinal tract of the trigerminal nerve (Fig 1D) In ... points relevant to the whole CNS Observations concerning of the forebrain are contained in the first of our two papers (Park et al, 2002) NGF was expressed in the developing brainstem and spinal ... and CNTF- immunoreactivity in the developing forebrain of Mongolian gerbil Korea J Vet Bes 2002, 42:137-146 Richardson P.M., and Ebendal T Nerve growth activities in rat peripheral nerve Brain...
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Báo cáo khoa học: "In vitro and in vivo gene therapy with CMV vector-mediated presumed dog b-nerve growth factor in pyridoxine-induced neuropathy dogs" pot
Ngày tải lên :
07/08/2014, 23:22
... pyridoxine intoxication in dogs In high doses, pyridoxine causes a selective degeneration of large and small myelinated sensory axons in the central and peripheral nerves, resulting in numbness and ... of pyridoxine injection On the fourth day of pyridoxine injection, all dogs held their hindlimb stiffly when standing These conditions were maintained until the end of the pyridoxine injection ... pyridoxine HCl and clioquinol (5-chloro-7-iodo-8hydroxyquinoline) in beagle dogs II Pathology Vet Pathol 1981, 18, 757-768 14 Hopkins AP, Gilliatt RW Motor and sensory nerve conduction velocity in...
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Báo cáo y học: " Aging, osteoarthritis and transforming growth factor-β signaling in cartilage" pps
Ngày tải lên :
09/08/2014, 07:20
... proteins to body morphogenetic proteins Cytokine Growth Factor Rev 2005, 16: 249-250 Lories RJU, Luyten FP: Bone morphogenetic protein signaling in joint homeostasis and disease Cytokine Growth ... transforming growth factor-beta signaling in cartilage of old mice: role in impaired repair capacity Arthritis Res Ther 2005, 7:R1338-R 1347 β ten Dijike P, Hill CS: New insights into TGF-β–Smad signaling ... Therapy Vol No Reddi R-Smads and the C-Smad have a highly conserved Mad homology domains (MH in the amino terminus and MH in the carboxy terminus) References Binding of the TGF-β isoforms to...
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Báo cáo y học: "Nerve growth factor and receptor expression in rheumatoid arthritis and spondyloarthritsi" potx
Ngày tải lên :
09/08/2014, 14:21
... four neurotrophins (nerve growth factor (NGF), brain-derived growth factor (BDNF), and neurotrophin (NT)-3, NT-4) and their corresponding receptors (high-affinity receptors tyrosine kinase (trk)A, ... receptors as determined in the SF cells are depicted in Table and outlined in detail by scatter plots in Figure (ligands) and Figure (receptors) As for the transcripts encoding the ligands, BDNF revealed ... mitogen-activated protein kinase pathway involving the c-jun N-terminal, the p38, and the extracellular-regulated protein kinases (ERK1/2) all of which have been identified in arthritis as well Interestingly,...
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Báo cáo y học: " Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc" pot
Ngày tải lên :
09/08/2014, 14:22
... were fixed in 4% paraformaldehyde/phosphate-buffered saline and processed into paraffin wax Five-micron sections from the tissue blocks were cut and stained with haematoxylin and eosin, and the ... [8-11] and more recently ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) families [12-16] In particular, we have shown that MMP-1, -3, -7, -9 and -13 are involved in matrix ... obtained following surgery for LBP This enabled us to investigate any differences in gene and protein expression between degenerate NP obtained from individuals who were asymptomatic and those individuals...
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Báo cáo y học: "Functional significance of nerve growth factor and its receptor (TrkA) in inflammatory arthritis" pps
Ngày tải lên :
12/08/2014, 14:21
... proliferating FLSs produce proteinases that degrade cartilage and underlying cortical bone [4] We noticed that proinflammatory cytokines upregulate NGF/TrkA in FLSs, NGF/TrkA is upregulated in FLSs of in ammatory ... in DMEM only, and stimulated cells were cultured in DMEM with cytokines tumor necrosis factor-alpha (TNF-α) (1 ng/mL) and interleukin-1-beta (IL-1β) (1 ng/mL) Data are expressed as mean ± standard ... the in ammatory and proliferative cascades of PsA and RA Abbreviations ELISA, enzyme-linked immunosorbent assay; FLS, fibroblast-like synoviocyte; NGF, nerve growth factor; NGF-R, nerve growth...
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Báo cáo y học: " Open Access Growth factor signaling in lung morphogenetic centers: automaticity, stereotypy and symmetry" doc
Ngày tải lên :
13/08/2014, 13:20
... activated TGFβ ligand is further regulated by soluble binding proteins such as Decorin, as well as by binding to matrix proteins such as Fibrillin Cognate receptor affinity for ligand binding may also ... by ligand binding include Ras-ERK, Rho-JNK, RhoA-p160RCCK, TAK1-p38MAPK and PP2A-S6 kinase Ligand binding also facilitates phosphorylation and activation of the TGFβ IR serine-threonine kinase ... Sprouty would inhibit the formation of specific signaling complexes downstream from tyrosine-kinase receptors resulting in modulation and co-ordination of cell growth and development during organogenesis...
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Tài liệu Báo cáo khoa học: Osmotic stress sensing and signaling in fishes doc
Ngày tải lên :
18/02/2014, 16:20
... place in the order of minutes to hours and involves many hormones, including arginine vasopressin, angiotensin II, natriuretic peptides, vasoactive intestinal peptide, urotensin II, insulin and ... (within h) induced by hyperosmotic stress, indicating a role of this mitogenactivated protein kinase kinase kinase interacting protein for osmosensory signal transduction in fish 14-3-3 proteins ... mitogen-activated protein kinase kinase kinase interacting protein (TAK binding protein ¼ TAB 2), as an IEG during hyperosmotic stress in tilapia gill epithelium [26] This gene is transiently and very rapidly...
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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx
Ngày tải lên :
18/02/2014, 16:20
... Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... Osmosensing and signaling in mammalian cell function F Schliess et al [7] On the other hand, hyperosmotic shrinkage prevents insulin-induced hepatocyte swelling and proteolysis inhibition, indicating ... [12–14] Integrin-inhibitory peptides exhibiting an arginine-glycine-aspartic acid (RGD) motif abolish hypoosmotic osmosignaling towards Src-type kinases, mitogen-activated protein kinases (MAPKs) and...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
Ngày tải lên :
18/02/2014, 16:20
... SO (1993) ADP- and thapsigargin-evoked Ca2+ entry and protein-tyrosine phosphorylation are inhibited by the tyrosine kinase inhibitors genistein and methyl-2,5-dihydroxycinnamate in fura-2-loaded ... work investigating the interaction of P2Y12 with P2Y1 signaling [25,40] We find that P2Y12 enhances the thrombin-induced Ca2+ response in a way not only involving adenylyl cyclase ⁄ PKA inhibition, ... response, which in turn Dual P2Y12 signaling in thrombin-stimulated platelets mediates thrombin generation, this indicates that P2Y12 signaling via PI3-Kb plays a regulating role in the positive...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
Ngày tải lên :
19/02/2014, 16:20
... barbed-end actin and determine the level and distribution of F-actin [34] Therefore, it is tempting to speculate that interactions between monomeric G-actin and actin-binding proteins are a potential ... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thrombin, dexamethasone, angiotensin II and prostaglandins stimulate ... for 30 and then fixed, permeabilized and stained for F-actin with rhodamine-conjugated phalloidin The effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the...
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